Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata
- Autores
- Wulff, Juan Pedro; Traverso, Lucila M.; Latorre Estivalis, Jose M.; Segura, Diego Fernando; Lanzavecchia, Silvia Beatriz
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Diachasmimorpha longicaudata is a hymenopteran fruit fly endoparasitoid. Females of this species find their hosts for oviposition by using complex sensorial mechanisms in response to physical and chemical stimuli associated with the host and host habitat. Ecological and behavioral aspects related to host-seeking behavior for oviposition have been extensively studied in D. longicaudata, including the identification of volatile organic compounds acting as attractants to females. In this sense, molecular mechanisms of chemoreception have been explored in this species, including a preliminary characterization of odorant-binding proteins (OBPs), chemosensory proteins (CSPs) and odorant receptors (ORs), among other proteins. Functional assays on OBP and CSP have been conducted as a first approach to identify molecular mechanisms associated with the female host-seeking behavior for oviposition. The aims of the present study were to identify the D. longicaudata sensory gene repertoire expressed in the antenna of sexually mature and mated individuals of both sexes, and subsequently, characterize transcripts differentially expressed in the antennae of females to identify candidate genes associated with the female host-seeking behavior for oviposition. Results: A total of 33,745 predicted protein-coding sequences were obtained from a de novo antennal transcriptome assembly. Ten sensory-related gene families were annotated as follows: 222 ORs, 44 ionotropic receptors (IRs), 25 gustatory receptors (GRs), 9 CSPs, 13 OBPs, 2 ammonium transporters (AMTs), 8 pickpocket (PPKs) receptors, 16 transient receptor potential (TRP) channels, 12 CD36/SNMPs and 3 Niemann-Pick type C2 like proteins (NPC2-like). The differential expression analysis revealed 237 and 151 transcripts up- and downregulated, respectively, between the female and male antennae. Ninety-seven differentially expressed transcripts corresponded to sensory-related genes including 88 transcripts being upregulated (87 ORs and one TRP) and nine downregulated (six ORs, two CSPs and one OBP) in females compared to males. Conclusions: The sensory gene repertoire of D. longicaudata was similar to that of other taxonomically related parasitoid wasps. We identified a high number of ORs upregulated in the female antenna. These results may indicate that this gene family has a central role in the chemoreception of sexually mature females during the search for hosts and host habitats for reproductive purposes.
Instituto de Genética
Fil: Wulff, Juan Pedro. North Carolina State University. Entomology and Plant Pathology; Estados Unidos
Fil: Traverso, Lucila M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro Regional de Estudios Genómicos. Laboratorio de Neurobiología de Insectos; Argentina
Fil: Traverso, Lucila M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Latorre Estivalis, Jose M. Universidad de Buenos Aires. Instituto de Fisiología. Laboratorio de Insectos Sociales, Biología Molecular y Neurociencias; Argentina
Fil: Latorre Estivalis, Jose M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina - Fuente
- BMC Genomics 25 (1) : 147 (Febrero 2024)
- Materia
-
Candidate Genes
Gene Expression
Receptors
Parasitoids
Tephritidae
Genes Candidatos
Expresión Génica
Receptor
Parasitoides
Biosteres longicaudatus
Secuencia de ARN
RNA Sequence
Diachasmimorpha longicaudata - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/17219
Ver los metadatos del registro completo
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Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudataWulff, Juan PedroTraverso, Lucila M.Latorre Estivalis, Jose M.Segura, Diego FernandoLanzavecchia, Silvia BeatrizCandidate GenesGene ExpressionReceptorsParasitoidsTephritidaeGenes CandidatosExpresión GénicaReceptorParasitoidesBiosteres longicaudatusSecuencia de ARNRNA SequenceDiachasmimorpha longicaudataBackground: Diachasmimorpha longicaudata is a hymenopteran fruit fly endoparasitoid. Females of this species find their hosts for oviposition by using complex sensorial mechanisms in response to physical and chemical stimuli associated with the host and host habitat. Ecological and behavioral aspects related to host-seeking behavior for oviposition have been extensively studied in D. longicaudata, including the identification of volatile organic compounds acting as attractants to females. In this sense, molecular mechanisms of chemoreception have been explored in this species, including a preliminary characterization of odorant-binding proteins (OBPs), chemosensory proteins (CSPs) and odorant receptors (ORs), among other proteins. Functional assays on OBP and CSP have been conducted as a first approach to identify molecular mechanisms associated with the female host-seeking behavior for oviposition. The aims of the present study were to identify the D. longicaudata sensory gene repertoire expressed in the antenna of sexually mature and mated individuals of both sexes, and subsequently, characterize transcripts differentially expressed in the antennae of females to identify candidate genes associated with the female host-seeking behavior for oviposition. Results: A total of 33,745 predicted protein-coding sequences were obtained from a de novo antennal transcriptome assembly. Ten sensory-related gene families were annotated as follows: 222 ORs, 44 ionotropic receptors (IRs), 25 gustatory receptors (GRs), 9 CSPs, 13 OBPs, 2 ammonium transporters (AMTs), 8 pickpocket (PPKs) receptors, 16 transient receptor potential (TRP) channels, 12 CD36/SNMPs and 3 Niemann-Pick type C2 like proteins (NPC2-like). The differential expression analysis revealed 237 and 151 transcripts up- and downregulated, respectively, between the female and male antennae. Ninety-seven differentially expressed transcripts corresponded to sensory-related genes including 88 transcripts being upregulated (87 ORs and one TRP) and nine downregulated (six ORs, two CSPs and one OBP) in females compared to males. Conclusions: The sensory gene repertoire of D. longicaudata was similar to that of other taxonomically related parasitoid wasps. We identified a high number of ORs upregulated in the female antenna. These results may indicate that this gene family has a central role in the chemoreception of sexually mature females during the search for hosts and host habitats for reproductive purposes.Instituto de GenéticaFil: Wulff, Juan Pedro. North Carolina State University. Entomology and Plant Pathology; Estados UnidosFil: Traverso, Lucila M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro Regional de Estudios Genómicos. Laboratorio de Neurobiología de Insectos; ArgentinaFil: Traverso, Lucila M. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Latorre Estivalis, Jose M. Universidad de Buenos Aires. Instituto de Fisiología. Laboratorio de Insectos Sociales, Biología Molecular y Neurociencias; ArgentinaFil: Latorre Estivalis, Jose M. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; ArgentinaBioMed Central2024-03-26T14:27:26Z2024-03-26T14:27:26Z2024-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/17219https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10034-61471-2164https://doi.org/10.1186/s12864-024-10034-6BMC Genomics 25 (1) : 147 (Febrero 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:26Zoai:localhost:20.500.12123/17219instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:26.544INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata |
title |
Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata |
spellingShingle |
Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata Wulff, Juan Pedro Candidate Genes Gene Expression Receptors Parasitoids Tephritidae Genes Candidatos Expresión Génica Receptor Parasitoides Biosteres longicaudatus Secuencia de ARN RNA Sequence Diachasmimorpha longicaudata |
title_short |
Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata |
title_full |
Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata |
title_fullStr |
Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata |
title_full_unstemmed |
Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata |
title_sort |
Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata |
dc.creator.none.fl_str_mv |
Wulff, Juan Pedro Traverso, Lucila M. Latorre Estivalis, Jose M. Segura, Diego Fernando Lanzavecchia, Silvia Beatriz |
author |
Wulff, Juan Pedro |
author_facet |
Wulff, Juan Pedro Traverso, Lucila M. Latorre Estivalis, Jose M. Segura, Diego Fernando Lanzavecchia, Silvia Beatriz |
author_role |
author |
author2 |
Traverso, Lucila M. Latorre Estivalis, Jose M. Segura, Diego Fernando Lanzavecchia, Silvia Beatriz |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Candidate Genes Gene Expression Receptors Parasitoids Tephritidae Genes Candidatos Expresión Génica Receptor Parasitoides Biosteres longicaudatus Secuencia de ARN RNA Sequence Diachasmimorpha longicaudata |
topic |
Candidate Genes Gene Expression Receptors Parasitoids Tephritidae Genes Candidatos Expresión Génica Receptor Parasitoides Biosteres longicaudatus Secuencia de ARN RNA Sequence Diachasmimorpha longicaudata |
dc.description.none.fl_txt_mv |
Background: Diachasmimorpha longicaudata is a hymenopteran fruit fly endoparasitoid. Females of this species find their hosts for oviposition by using complex sensorial mechanisms in response to physical and chemical stimuli associated with the host and host habitat. Ecological and behavioral aspects related to host-seeking behavior for oviposition have been extensively studied in D. longicaudata, including the identification of volatile organic compounds acting as attractants to females. In this sense, molecular mechanisms of chemoreception have been explored in this species, including a preliminary characterization of odorant-binding proteins (OBPs), chemosensory proteins (CSPs) and odorant receptors (ORs), among other proteins. Functional assays on OBP and CSP have been conducted as a first approach to identify molecular mechanisms associated with the female host-seeking behavior for oviposition. The aims of the present study were to identify the D. longicaudata sensory gene repertoire expressed in the antenna of sexually mature and mated individuals of both sexes, and subsequently, characterize transcripts differentially expressed in the antennae of females to identify candidate genes associated with the female host-seeking behavior for oviposition. Results: A total of 33,745 predicted protein-coding sequences were obtained from a de novo antennal transcriptome assembly. Ten sensory-related gene families were annotated as follows: 222 ORs, 44 ionotropic receptors (IRs), 25 gustatory receptors (GRs), 9 CSPs, 13 OBPs, 2 ammonium transporters (AMTs), 8 pickpocket (PPKs) receptors, 16 transient receptor potential (TRP) channels, 12 CD36/SNMPs and 3 Niemann-Pick type C2 like proteins (NPC2-like). The differential expression analysis revealed 237 and 151 transcripts up- and downregulated, respectively, between the female and male antennae. Ninety-seven differentially expressed transcripts corresponded to sensory-related genes including 88 transcripts being upregulated (87 ORs and one TRP) and nine downregulated (six ORs, two CSPs and one OBP) in females compared to males. Conclusions: The sensory gene repertoire of D. longicaudata was similar to that of other taxonomically related parasitoid wasps. We identified a high number of ORs upregulated in the female antenna. These results may indicate that this gene family has a central role in the chemoreception of sexually mature females during the search for hosts and host habitats for reproductive purposes. Instituto de Genética Fil: Wulff, Juan Pedro. North Carolina State University. Entomology and Plant Pathology; Estados Unidos Fil: Traverso, Lucila M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro Regional de Estudios Genómicos. Laboratorio de Neurobiología de Insectos; Argentina Fil: Traverso, Lucila M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Latorre Estivalis, Jose M. Universidad de Buenos Aires. Instituto de Fisiología. Laboratorio de Insectos Sociales, Biología Molecular y Neurociencias; Argentina Fil: Latorre Estivalis, Jose M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina |
description |
Background: Diachasmimorpha longicaudata is a hymenopteran fruit fly endoparasitoid. Females of this species find their hosts for oviposition by using complex sensorial mechanisms in response to physical and chemical stimuli associated with the host and host habitat. Ecological and behavioral aspects related to host-seeking behavior for oviposition have been extensively studied in D. longicaudata, including the identification of volatile organic compounds acting as attractants to females. In this sense, molecular mechanisms of chemoreception have been explored in this species, including a preliminary characterization of odorant-binding proteins (OBPs), chemosensory proteins (CSPs) and odorant receptors (ORs), among other proteins. Functional assays on OBP and CSP have been conducted as a first approach to identify molecular mechanisms associated with the female host-seeking behavior for oviposition. The aims of the present study were to identify the D. longicaudata sensory gene repertoire expressed in the antenna of sexually mature and mated individuals of both sexes, and subsequently, characterize transcripts differentially expressed in the antennae of females to identify candidate genes associated with the female host-seeking behavior for oviposition. Results: A total of 33,745 predicted protein-coding sequences were obtained from a de novo antennal transcriptome assembly. Ten sensory-related gene families were annotated as follows: 222 ORs, 44 ionotropic receptors (IRs), 25 gustatory receptors (GRs), 9 CSPs, 13 OBPs, 2 ammonium transporters (AMTs), 8 pickpocket (PPKs) receptors, 16 transient receptor potential (TRP) channels, 12 CD36/SNMPs and 3 Niemann-Pick type C2 like proteins (NPC2-like). The differential expression analysis revealed 237 and 151 transcripts up- and downregulated, respectively, between the female and male antennae. Ninety-seven differentially expressed transcripts corresponded to sensory-related genes including 88 transcripts being upregulated (87 ORs and one TRP) and nine downregulated (six ORs, two CSPs and one OBP) in females compared to males. Conclusions: The sensory gene repertoire of D. longicaudata was similar to that of other taxonomically related parasitoid wasps. We identified a high number of ORs upregulated in the female antenna. These results may indicate that this gene family has a central role in the chemoreception of sexually mature females during the search for hosts and host habitats for reproductive purposes. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-03-26T14:27:26Z 2024-03-26T14:27:26Z 2024-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/17219 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10034-6 1471-2164 https://doi.org/10.1186/s12864-024-10034-6 |
url |
http://hdl.handle.net/20.500.12123/17219 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10034-6 https://doi.org/10.1186/s12864-024-10034-6 |
identifier_str_mv |
1471-2164 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central |
publisher.none.fl_str_mv |
BioMed Central |
dc.source.none.fl_str_mv |
BMC Genomics 25 (1) : 147 (Febrero 2024) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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