Transcriptional regulation of the anthocyanin biosynthesis pathway in developing grapevine berries in cultivar ‘Malbec’ by putative R2R3 MYB negative regulators

Autores
Muñoz, Claudio Javier; Fanzone, Martín Leandro; Lijavetzky, Diego
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In grapevine, proanthocyanins (PAs) and anthocyanins are obtained via the phenylpropanoid pathway. These metabolites are synthesized in specific tissues of the berry and accumulate in different developmental stages. The transcriptional control of the route is affected by transcription factors (TFs), mainly R2R3- Myb type, which can act as activators or repressors. Here we studied the fine regulation of the pathway by analysing the expression profiles of nine TFs operating as activators of the route as well as two main structural genes. In addition, we analysed the profiles of six R2R3-Myb subgroup 4 TFs described as repressors of the route (VviMybC2-L1, VviMybC2-L2, VviMybC2-L3, VviMyb4a, VviMyb4b and VviMyb4-like). The analysis of gene expression was carried out in five different development stages of the berry and in two berry tissues (skin and flesh) of cultivar ‘Malbec’. On the other hand, the putative R2R3-Myb repressors were analysed and compared in the tenturier cultivar ‘Alicante Bouschet’, in the same tissues and stages of development as ‘Malbec’. Concerning the R2R3-Myb repressors, our results indicate that in the ‘Malbec’ skins, VviMyb4a and VviMyb4b would act on the early genes of the route while VviMybC2-L2 and VviMyb4-like are co-expressed towards the end of the anthocyanin biosynthesis. In addition, the VviMybC2-L1 and VviMybC2-L3 are induced at two stages of development, first towards the end of the green phase of the berry, indicating the possible synergistic interaction by repressing the synthesis of PAs, then they are induced again towards maturation to inhibit the synthesis of anthocyanins. On the other hand, our results showed that the expression profiles of Myb repressor genes may explain the presence of colour in the flesh of Alicante Bouschet variety.
EEA Mendoza
Fil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fanzone, Martín Leandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Aromas y Sustancias Naturales; Argentina
Fil: Lijavetzky, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fuente
Scientia horticulturae 257 : 108663. (17 November 2019)
Materia
Vitis Vinífera
Variedades
Antocianinas
Sustancias de Crecimiento Vegetal
Factores de Transcripción
Varieties
Anthocyanins
Plant Growth Substances
Transcription Factors
Malbec
Proanthocyanidins
Myb Transcription Factors
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5675
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5675
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Transcriptional regulation of the anthocyanin biosynthesis pathway in developing grapevine berries in cultivar ‘Malbec’ by putative R2R3 MYB negative regulatorsMuñoz, Claudio JavierFanzone, Martín LeandroLijavetzky, DiegoVitis ViníferaVariedadesAntocianinasSustancias de Crecimiento VegetalFactores de TranscripciónVarietiesAnthocyaninsPlant Growth SubstancesTranscription FactorsMalbecProanthocyanidinsMyb Transcription FactorsIn grapevine, proanthocyanins (PAs) and anthocyanins are obtained via the phenylpropanoid pathway. These metabolites are synthesized in specific tissues of the berry and accumulate in different developmental stages. The transcriptional control of the route is affected by transcription factors (TFs), mainly R2R3- Myb type, which can act as activators or repressors. Here we studied the fine regulation of the pathway by analysing the expression profiles of nine TFs operating as activators of the route as well as two main structural genes. In addition, we analysed the profiles of six R2R3-Myb subgroup 4 TFs described as repressors of the route (VviMybC2-L1, VviMybC2-L2, VviMybC2-L3, VviMyb4a, VviMyb4b and VviMyb4-like). The analysis of gene expression was carried out in five different development stages of the berry and in two berry tissues (skin and flesh) of cultivar ‘Malbec’. On the other hand, the putative R2R3-Myb repressors were analysed and compared in the tenturier cultivar ‘Alicante Bouschet’, in the same tissues and stages of development as ‘Malbec’. Concerning the R2R3-Myb repressors, our results indicate that in the ‘Malbec’ skins, VviMyb4a and VviMyb4b would act on the early genes of the route while VviMybC2-L2 and VviMyb4-like are co-expressed towards the end of the anthocyanin biosynthesis. In addition, the VviMybC2-L1 and VviMybC2-L3 are induced at two stages of development, first towards the end of the green phase of the berry, indicating the possible synergistic interaction by repressing the synthesis of PAs, then they are induced again towards maturation to inhibit the synthesis of anthocyanins. On the other hand, our results showed that the expression profiles of Myb repressor genes may explain the presence of colour in the flesh of Alicante Bouschet variety.EEA MendozaFil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Fanzone, Martín Leandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Aromas y Sustancias Naturales; ArgentinaFil: Lijavetzky, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaElsevier2019-08-23T12:09:08Z2019-08-23T12:09:08Z2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/5675https://www.sciencedirect.com/science/article/pii/S0304423819305497?via%3Dihub0304-4238https://doi.org/10.1016/j.scienta.2019.108663Scientia horticulturae 257 : 108663. (17 November 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:08Zoai:localhost:20.500.12123/5675instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:09.106INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Transcriptional regulation of the anthocyanin biosynthesis pathway in developing grapevine berries in cultivar ‘Malbec’ by putative R2R3 MYB negative regulators
title Transcriptional regulation of the anthocyanin biosynthesis pathway in developing grapevine berries in cultivar ‘Malbec’ by putative R2R3 MYB negative regulators
spellingShingle Transcriptional regulation of the anthocyanin biosynthesis pathway in developing grapevine berries in cultivar ‘Malbec’ by putative R2R3 MYB negative regulators
Muñoz, Claudio Javier
Vitis Vinífera
Variedades
Antocianinas
Sustancias de Crecimiento Vegetal
Factores de Transcripción
Varieties
Anthocyanins
Plant Growth Substances
Transcription Factors
Malbec
Proanthocyanidins
Myb Transcription Factors
title_short Transcriptional regulation of the anthocyanin biosynthesis pathway in developing grapevine berries in cultivar ‘Malbec’ by putative R2R3 MYB negative regulators
title_full Transcriptional regulation of the anthocyanin biosynthesis pathway in developing grapevine berries in cultivar ‘Malbec’ by putative R2R3 MYB negative regulators
title_fullStr Transcriptional regulation of the anthocyanin biosynthesis pathway in developing grapevine berries in cultivar ‘Malbec’ by putative R2R3 MYB negative regulators
title_full_unstemmed Transcriptional regulation of the anthocyanin biosynthesis pathway in developing grapevine berries in cultivar ‘Malbec’ by putative R2R3 MYB negative regulators
title_sort Transcriptional regulation of the anthocyanin biosynthesis pathway in developing grapevine berries in cultivar ‘Malbec’ by putative R2R3 MYB negative regulators
dc.creator.none.fl_str_mv Muñoz, Claudio Javier
Fanzone, Martín Leandro
Lijavetzky, Diego
author Muñoz, Claudio Javier
author_facet Muñoz, Claudio Javier
Fanzone, Martín Leandro
Lijavetzky, Diego
author_role author
author2 Fanzone, Martín Leandro
Lijavetzky, Diego
author2_role author
author
dc.subject.none.fl_str_mv Vitis Vinífera
Variedades
Antocianinas
Sustancias de Crecimiento Vegetal
Factores de Transcripción
Varieties
Anthocyanins
Plant Growth Substances
Transcription Factors
Malbec
Proanthocyanidins
Myb Transcription Factors
topic Vitis Vinífera
Variedades
Antocianinas
Sustancias de Crecimiento Vegetal
Factores de Transcripción
Varieties
Anthocyanins
Plant Growth Substances
Transcription Factors
Malbec
Proanthocyanidins
Myb Transcription Factors
dc.description.none.fl_txt_mv In grapevine, proanthocyanins (PAs) and anthocyanins are obtained via the phenylpropanoid pathway. These metabolites are synthesized in specific tissues of the berry and accumulate in different developmental stages. The transcriptional control of the route is affected by transcription factors (TFs), mainly R2R3- Myb type, which can act as activators or repressors. Here we studied the fine regulation of the pathway by analysing the expression profiles of nine TFs operating as activators of the route as well as two main structural genes. In addition, we analysed the profiles of six R2R3-Myb subgroup 4 TFs described as repressors of the route (VviMybC2-L1, VviMybC2-L2, VviMybC2-L3, VviMyb4a, VviMyb4b and VviMyb4-like). The analysis of gene expression was carried out in five different development stages of the berry and in two berry tissues (skin and flesh) of cultivar ‘Malbec’. On the other hand, the putative R2R3-Myb repressors were analysed and compared in the tenturier cultivar ‘Alicante Bouschet’, in the same tissues and stages of development as ‘Malbec’. Concerning the R2R3-Myb repressors, our results indicate that in the ‘Malbec’ skins, VviMyb4a and VviMyb4b would act on the early genes of the route while VviMybC2-L2 and VviMyb4-like are co-expressed towards the end of the anthocyanin biosynthesis. In addition, the VviMybC2-L1 and VviMybC2-L3 are induced at two stages of development, first towards the end of the green phase of the berry, indicating the possible synergistic interaction by repressing the synthesis of PAs, then they are induced again towards maturation to inhibit the synthesis of anthocyanins. On the other hand, our results showed that the expression profiles of Myb repressor genes may explain the presence of colour in the flesh of Alicante Bouschet variety.
EEA Mendoza
Fil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fanzone, Martín Leandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Aromas y Sustancias Naturales; Argentina
Fil: Lijavetzky, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description In grapevine, proanthocyanins (PAs) and anthocyanins are obtained via the phenylpropanoid pathway. These metabolites are synthesized in specific tissues of the berry and accumulate in different developmental stages. The transcriptional control of the route is affected by transcription factors (TFs), mainly R2R3- Myb type, which can act as activators or repressors. Here we studied the fine regulation of the pathway by analysing the expression profiles of nine TFs operating as activators of the route as well as two main structural genes. In addition, we analysed the profiles of six R2R3-Myb subgroup 4 TFs described as repressors of the route (VviMybC2-L1, VviMybC2-L2, VviMybC2-L3, VviMyb4a, VviMyb4b and VviMyb4-like). The analysis of gene expression was carried out in five different development stages of the berry and in two berry tissues (skin and flesh) of cultivar ‘Malbec’. On the other hand, the putative R2R3-Myb repressors were analysed and compared in the tenturier cultivar ‘Alicante Bouschet’, in the same tissues and stages of development as ‘Malbec’. Concerning the R2R3-Myb repressors, our results indicate that in the ‘Malbec’ skins, VviMyb4a and VviMyb4b would act on the early genes of the route while VviMybC2-L2 and VviMyb4-like are co-expressed towards the end of the anthocyanin biosynthesis. In addition, the VviMybC2-L1 and VviMybC2-L3 are induced at two stages of development, first towards the end of the green phase of the berry, indicating the possible synergistic interaction by repressing the synthesis of PAs, then they are induced again towards maturation to inhibit the synthesis of anthocyanins. On the other hand, our results showed that the expression profiles of Myb repressor genes may explain the presence of colour in the flesh of Alicante Bouschet variety.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-23T12:09:08Z
2019-08-23T12:09:08Z
2019-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/5675
https://www.sciencedirect.com/science/article/pii/S0304423819305497?via%3Dihub
0304-4238
https://doi.org/10.1016/j.scienta.2019.108663
url http://hdl.handle.net/20.500.12123/5675
https://www.sciencedirect.com/science/article/pii/S0304423819305497?via%3Dihub
https://doi.org/10.1016/j.scienta.2019.108663
identifier_str_mv 0304-4238
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Scientia horticulturae 257 : 108663. (17 November 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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