Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment

Autores
Lifschitz, Adrian Luis; Nava, Santiago; Mangold, Atilio Jose; Imperiale, Fernanda Andrea; Ballent, Mariana; Canevari, Jose Tobias; Lanusse, Carlos Edmundo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Eprinomectin (EPM) is a macrocyclic lactone used against endo-ectoparasites without withdrawal time in milk and meat after its pour-on administration at 0.5 mg/kg. Previous experiments evaluated the efficacy of EPM against Rhipicephalus (Boophilus) microplus in cattle. This study assessed EPM efficacy against R. (B.) microplus after topical administration at two dose rates and investigated the relationship between EPM systemic exposure in the host and drug concentrations accumulated in ticks recovered from treated animals. A standardized pharmaco-parasitological study was performed in two phases. In phase 1 eighteen Braford cattle naturally infected with R. (B.) microplus were divided into three experimental groups with a similar level of infestation (Kruskal–Wallis test, P > 0.05): control group and treated groups with EPM pour-on (1 and 1.5 mg/kg). Samples of heparinized blood and ticks at different life stages were taken between 0 and 21 days (d) post-administration to measure EPM concentrations by HPLC. The efficacy trial (phase 2) included eighteen Braford calves naturally infected with R. (B.) microplus divided into control group and 1 mg/kg and 1.5 mg/kg EPM treated groups. Female ticks (4.5–8 mm) on cattle were counted between 1 and 23 days post-treatment to evaluate the efficacy of EPM. The reproductive efficiency index (REI) and the fertility efficiency index (FEI) were evaluated. Plasma concentrations of EPM showed a linear relationship with the level of dose rate administered. Peak plasma concentrations were within a range between 13.8 and 90 ng/ml, which guarantee milk drug concentrations below the maximum residues level. High EPM concentrations were detected in ticks. EPM concentrations in R. (B.) microplus were correlated to plasma concentrations between 1.25 days and 21 days post-administration (r 0.84; P < 0.05). EPM efficacy calculated using the Henderson–Tilton formula was 98.9% and 99.1% (7 days post-administration) and 100% (23 days post-administration) after EPM treatment at 1 and 1.5 mg/kg, respectively. EPM administered at 1.5 mg/kg also showed a significantly higher deleterious effect on tick fertility as measured by FEI (P < 0.01). Therefore, treatment with EPM may be useful for controlling ticks in cattle, particularly in dairy production systems.
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Imperiale, F. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina
Fil: Ballent, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Canevari, Jose Tobias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigación Veterinaria de Tandil; Argentina
Fuente
Veterinary parasitology 215 : 11-16. (January 2016)
Materia
Rhipicephalus
Ganado Bovino
Cattle
Pharmacokinetic
Farmacocinética
Rhipicephalus (Boophilus) Microplus
Garrapatas
Eprinomectina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1446
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1446
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessmentLifschitz, Adrian LuisNava, SantiagoMangold, Atilio JoseImperiale, Fernanda AndreaBallent, MarianaCanevari, Jose TobiasLanusse, Carlos EdmundoRhipicephalusGanado BovinoCattlePharmacokineticFarmacocinéticaRhipicephalus (Boophilus) MicroplusGarrapatasEprinomectinaEprinomectin (EPM) is a macrocyclic lactone used against endo-ectoparasites without withdrawal time in milk and meat after its pour-on administration at 0.5 mg/kg. Previous experiments evaluated the efficacy of EPM against Rhipicephalus (Boophilus) microplus in cattle. This study assessed EPM efficacy against R. (B.) microplus after topical administration at two dose rates and investigated the relationship between EPM systemic exposure in the host and drug concentrations accumulated in ticks recovered from treated animals. A standardized pharmaco-parasitological study was performed in two phases. In phase 1 eighteen Braford cattle naturally infected with R. (B.) microplus were divided into three experimental groups with a similar level of infestation (Kruskal–Wallis test, P > 0.05): control group and treated groups with EPM pour-on (1 and 1.5 mg/kg). Samples of heparinized blood and ticks at different life stages were taken between 0 and 21 days (d) post-administration to measure EPM concentrations by HPLC. The efficacy trial (phase 2) included eighteen Braford calves naturally infected with R. (B.) microplus divided into control group and 1 mg/kg and 1.5 mg/kg EPM treated groups. Female ticks (4.5–8 mm) on cattle were counted between 1 and 23 days post-treatment to evaluate the efficacy of EPM. The reproductive efficiency index (REI) and the fertility efficiency index (FEI) were evaluated. Plasma concentrations of EPM showed a linear relationship with the level of dose rate administered. Peak plasma concentrations were within a range between 13.8 and 90 ng/ml, which guarantee milk drug concentrations below the maximum residues level. High EPM concentrations were detected in ticks. EPM concentrations in R. (B.) microplus were correlated to plasma concentrations between 1.25 days and 21 days post-administration (r 0.84; P < 0.05). EPM efficacy calculated using the Henderson–Tilton formula was 98.9% and 99.1% (7 days post-administration) and 100% (23 days post-administration) after EPM treatment at 1 and 1.5 mg/kg, respectively. EPM administered at 1.5 mg/kg also showed a significantly higher deleterious effect on tick fertility as measured by FEI (P < 0.01). Therefore, treatment with EPM may be useful for controlling ticks in cattle, particularly in dairy production systems.Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Imperiale, F. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; ArgentinaFil: Ballent, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Canevari, Jose Tobias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigación Veterinaria de Tandil; Argentina2017-10-10T13:01:05Z2017-10-10T13:01:05Z2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1446http://www.sciencedirect.com/science/article/pii/S03044017153007280304-4017https://doi.org/10.1016/j.vetpar.2015.11.005Veterinary parasitology 215 : 11-16. (January 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:05Zoai:localhost:20.500.12123/1446instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:06.284INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment
title Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment
spellingShingle Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment
Lifschitz, Adrian Luis
Rhipicephalus
Ganado Bovino
Cattle
Pharmacokinetic
Farmacocinética
Rhipicephalus (Boophilus) Microplus
Garrapatas
Eprinomectina
title_short Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment
title_full Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment
title_fullStr Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment
title_full_unstemmed Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment
title_sort Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus : pharmacokinetic and efficacy assessment
dc.creator.none.fl_str_mv Lifschitz, Adrian Luis
Nava, Santiago
Mangold, Atilio Jose
Imperiale, Fernanda Andrea
Ballent, Mariana
Canevari, Jose Tobias
Lanusse, Carlos Edmundo
author Lifschitz, Adrian Luis
author_facet Lifschitz, Adrian Luis
Nava, Santiago
Mangold, Atilio Jose
Imperiale, Fernanda Andrea
Ballent, Mariana
Canevari, Jose Tobias
Lanusse, Carlos Edmundo
author_role author
author2 Nava, Santiago
Mangold, Atilio Jose
Imperiale, Fernanda Andrea
Ballent, Mariana
Canevari, Jose Tobias
Lanusse, Carlos Edmundo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Rhipicephalus
Ganado Bovino
Cattle
Pharmacokinetic
Farmacocinética
Rhipicephalus (Boophilus) Microplus
Garrapatas
Eprinomectina
topic Rhipicephalus
Ganado Bovino
Cattle
Pharmacokinetic
Farmacocinética
Rhipicephalus (Boophilus) Microplus
Garrapatas
Eprinomectina
dc.description.none.fl_txt_mv Eprinomectin (EPM) is a macrocyclic lactone used against endo-ectoparasites without withdrawal time in milk and meat after its pour-on administration at 0.5 mg/kg. Previous experiments evaluated the efficacy of EPM against Rhipicephalus (Boophilus) microplus in cattle. This study assessed EPM efficacy against R. (B.) microplus after topical administration at two dose rates and investigated the relationship between EPM systemic exposure in the host and drug concentrations accumulated in ticks recovered from treated animals. A standardized pharmaco-parasitological study was performed in two phases. In phase 1 eighteen Braford cattle naturally infected with R. (B.) microplus were divided into three experimental groups with a similar level of infestation (Kruskal–Wallis test, P > 0.05): control group and treated groups with EPM pour-on (1 and 1.5 mg/kg). Samples of heparinized blood and ticks at different life stages were taken between 0 and 21 days (d) post-administration to measure EPM concentrations by HPLC. The efficacy trial (phase 2) included eighteen Braford calves naturally infected with R. (B.) microplus divided into control group and 1 mg/kg and 1.5 mg/kg EPM treated groups. Female ticks (4.5–8 mm) on cattle were counted between 1 and 23 days post-treatment to evaluate the efficacy of EPM. The reproductive efficiency index (REI) and the fertility efficiency index (FEI) were evaluated. Plasma concentrations of EPM showed a linear relationship with the level of dose rate administered. Peak plasma concentrations were within a range between 13.8 and 90 ng/ml, which guarantee milk drug concentrations below the maximum residues level. High EPM concentrations were detected in ticks. EPM concentrations in R. (B.) microplus were correlated to plasma concentrations between 1.25 days and 21 days post-administration (r 0.84; P < 0.05). EPM efficacy calculated using the Henderson–Tilton formula was 98.9% and 99.1% (7 days post-administration) and 100% (23 days post-administration) after EPM treatment at 1 and 1.5 mg/kg, respectively. EPM administered at 1.5 mg/kg also showed a significantly higher deleterious effect on tick fertility as measured by FEI (P < 0.01). Therefore, treatment with EPM may be useful for controlling ticks in cattle, particularly in dairy production systems.
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Imperiale, F. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires.. Facultad de Ciencias Veterinarias; Argentina
Fil: Ballent, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET- Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Canevari, Jose Tobias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigación Veterinaria de Tandil; Argentina
description Eprinomectin (EPM) is a macrocyclic lactone used against endo-ectoparasites without withdrawal time in milk and meat after its pour-on administration at 0.5 mg/kg. Previous experiments evaluated the efficacy of EPM against Rhipicephalus (Boophilus) microplus in cattle. This study assessed EPM efficacy against R. (B.) microplus after topical administration at two dose rates and investigated the relationship between EPM systemic exposure in the host and drug concentrations accumulated in ticks recovered from treated animals. A standardized pharmaco-parasitological study was performed in two phases. In phase 1 eighteen Braford cattle naturally infected with R. (B.) microplus were divided into three experimental groups with a similar level of infestation (Kruskal–Wallis test, P > 0.05): control group and treated groups with EPM pour-on (1 and 1.5 mg/kg). Samples of heparinized blood and ticks at different life stages were taken between 0 and 21 days (d) post-administration to measure EPM concentrations by HPLC. The efficacy trial (phase 2) included eighteen Braford calves naturally infected with R. (B.) microplus divided into control group and 1 mg/kg and 1.5 mg/kg EPM treated groups. Female ticks (4.5–8 mm) on cattle were counted between 1 and 23 days post-treatment to evaluate the efficacy of EPM. The reproductive efficiency index (REI) and the fertility efficiency index (FEI) were evaluated. Plasma concentrations of EPM showed a linear relationship with the level of dose rate administered. Peak plasma concentrations were within a range between 13.8 and 90 ng/ml, which guarantee milk drug concentrations below the maximum residues level. High EPM concentrations were detected in ticks. EPM concentrations in R. (B.) microplus were correlated to plasma concentrations between 1.25 days and 21 days post-administration (r 0.84; P < 0.05). EPM efficacy calculated using the Henderson–Tilton formula was 98.9% and 99.1% (7 days post-administration) and 100% (23 days post-administration) after EPM treatment at 1 and 1.5 mg/kg, respectively. EPM administered at 1.5 mg/kg also showed a significantly higher deleterious effect on tick fertility as measured by FEI (P < 0.01). Therefore, treatment with EPM may be useful for controlling ticks in cattle, particularly in dairy production systems.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
2017-10-10T13:01:05Z
2017-10-10T13:01:05Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1446
http://www.sciencedirect.com/science/article/pii/S0304401715300728
0304-4017
https://doi.org/10.1016/j.vetpar.2015.11.005
url http://hdl.handle.net/20.500.12123/1446
http://www.sciencedirect.com/science/article/pii/S0304401715300728
https://doi.org/10.1016/j.vetpar.2015.11.005
identifier_str_mv 0304-4017
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Veterinary parasitology 215 : 11-16. (January 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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