Crop-specific response of soil prokaryotic community to long-term intensification management: the importance of crop phase at sampling

Autores
Frene, Juan Pablo; Bacigaluppo, Silvina; Maury, Mariana; Ortiz, Jimena; Rivarola, Maximo Lisandro; Salvagiotti, Fernando; Faggioli, Valeria Soledad
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: The ecosystem services provided by soil microbial communities are critical for the resilience of agroecosystems, ensuring environmental conservation and food security. Long-term experiments comparing contrasting crop rotations are valuable tools for monitoring microbial responses, but they rarely include all crop phases within a single year. Therefore, the long-term agronomic impact may be masked by the immediate effect of the crop evaluated. In this study, we compared different crop rotations based on the Intensification Sequence Index (ISI), which considers the soil occupation time, and analyzed the impact of cover crops and two nitrogen fertilization strategies. Material and methods: We used an experiment initiated in 2006 with the following crop rotations: Soybean-Soybean, Maize-Wheat/Soybean, and Maize-Soybean-Wheat/Soybean. Soil samples were taken after the harvest of each summer crop phase (i.e., Soybean, Maize, Wheat/Soybean), and the soil prokaryotic community was monitored using 16S rRNA gene sequencing. Results and discussion: We observed that ISI and crop phase were the main predictors of microbial community composition, explaining 14.7% and 13.0% of the variation, respectively. Nitrogen fertilization had a minor effect (3.12%) and was detected only after maize sampling; cover crops had no significant effect. However, the presence of cover crops showed higher alpha diversity and an increased abundance of Proteobacteria. Maize enriched the abundance of certain taxa of Planctomycetes and Verrucomicrobia, while Soybean increased the abundance of Bacteroidetes and Proteobacteria. Comparatively, Soybean enriched Mucilaginibacter and Geobacter, while Wheat/Soybean enriched Brevundimonas and Roseimicrobium. Conclusion: Our results demonstrate that crop phase is as important as the long-term legacy of crop rotations in shaping the microbial community and that specific taxa responses are highly dependent on the crop phase surveyed.
EEA Marcos Juárez
Fil: Frene, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Bacigaluppo, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Oliveros; Argentina
Fil: Maury, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Ortiz, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rivarola, Maximo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salvagiotti, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Oliveros; Argentina
Fil: Faggioli, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fuente
Frontiers in Agronomy 6 : 1446404. (December 2024)
Materia
Cultivos
Suelo
Aplicación de Abonos
Abonos Nitrogenados
Plantas de Cobertura
Intensificación
Crops
Soil
Fertilizer Application
Nitrogen Fertilizers
Cover Plants
Intensification
Fertilización Nitrogenada
Cultivos de Cobertura
Nitrogen Fertilization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22681
Acceder
id INTADig_239198c9816a224055f29dadc54b398b
oai_identifier_str oai:localhost:20.500.12123/22681
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Crop-specific response of soil prokaryotic community to long-term intensification management: the importance of crop phase at samplingFrene, Juan PabloBacigaluppo, SilvinaMaury, MarianaOrtiz, JimenaRivarola, Maximo LisandroSalvagiotti, FernandoFaggioli, Valeria SoledadCultivosSueloAplicación de AbonosAbonos NitrogenadosPlantas de CoberturaIntensificaciónCropsSoilFertilizer ApplicationNitrogen FertilizersCover PlantsIntensificationFertilización NitrogenadaCultivos de CoberturaNitrogen FertilizationIntroduction: The ecosystem services provided by soil microbial communities are critical for the resilience of agroecosystems, ensuring environmental conservation and food security. Long-term experiments comparing contrasting crop rotations are valuable tools for monitoring microbial responses, but they rarely include all crop phases within a single year. Therefore, the long-term agronomic impact may be masked by the immediate effect of the crop evaluated. In this study, we compared different crop rotations based on the Intensification Sequence Index (ISI), which considers the soil occupation time, and analyzed the impact of cover crops and two nitrogen fertilization strategies. Material and methods: We used an experiment initiated in 2006 with the following crop rotations: Soybean-Soybean, Maize-Wheat/Soybean, and Maize-Soybean-Wheat/Soybean. Soil samples were taken after the harvest of each summer crop phase (i.e., Soybean, Maize, Wheat/Soybean), and the soil prokaryotic community was monitored using 16S rRNA gene sequencing. Results and discussion: We observed that ISI and crop phase were the main predictors of microbial community composition, explaining 14.7% and 13.0% of the variation, respectively. Nitrogen fertilization had a minor effect (3.12%) and was detected only after maize sampling; cover crops had no significant effect. However, the presence of cover crops showed higher alpha diversity and an increased abundance of Proteobacteria. Maize enriched the abundance of certain taxa of Planctomycetes and Verrucomicrobia, while Soybean increased the abundance of Bacteroidetes and Proteobacteria. Comparatively, Soybean enriched Mucilaginibacter and Geobacter, while Wheat/Soybean enriched Brevundimonas and Roseimicrobium. Conclusion: Our results demonstrate that crop phase is as important as the long-term legacy of crop rotations in shaping the microbial community and that specific taxa responses are highly dependent on the crop phase surveyed.EEA Marcos JuárezFil: Frene, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Bacigaluppo, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Oliveros; ArgentinaFil: Maury, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Ortiz, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Rivarola, Maximo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Salvagiotti, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Oliveros; ArgentinaFil: Faggioli, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFrontiers Media2025-06-13T13:08:34Z2025-06-13T13:08:34Z2024-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22681https://www.frontiersin.org/journals/agronomy/articles/10.3389/fagro.2024.1446404/full2673-3218https://doi.org/10.3389/fagro.2024.1446404Frontiers in Agronomy 6 : 1446404. (December 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-18T10:10:06Zoai:localhost:20.500.12123/22681instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:10:07.221INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Crop-specific response of soil prokaryotic community to long-term intensification management: the importance of crop phase at sampling
title Crop-specific response of soil prokaryotic community to long-term intensification management: the importance of crop phase at sampling
spellingShingle Crop-specific response of soil prokaryotic community to long-term intensification management: the importance of crop phase at sampling
Frene, Juan Pablo
Cultivos
Suelo
Aplicación de Abonos
Abonos Nitrogenados
Plantas de Cobertura
Intensificación
Crops
Soil
Fertilizer Application
Nitrogen Fertilizers
Cover Plants
Intensification
Fertilización Nitrogenada
Cultivos de Cobertura
Nitrogen Fertilization
title_short Crop-specific response of soil prokaryotic community to long-term intensification management: the importance of crop phase at sampling
title_full Crop-specific response of soil prokaryotic community to long-term intensification management: the importance of crop phase at sampling
title_fullStr Crop-specific response of soil prokaryotic community to long-term intensification management: the importance of crop phase at sampling
title_full_unstemmed Crop-specific response of soil prokaryotic community to long-term intensification management: the importance of crop phase at sampling
title_sort Crop-specific response of soil prokaryotic community to long-term intensification management: the importance of crop phase at sampling
dc.creator.none.fl_str_mv Frene, Juan Pablo
Bacigaluppo, Silvina
Maury, Mariana
Ortiz, Jimena
Rivarola, Maximo Lisandro
Salvagiotti, Fernando
Faggioli, Valeria Soledad
author Frene, Juan Pablo
author_facet Frene, Juan Pablo
Bacigaluppo, Silvina
Maury, Mariana
Ortiz, Jimena
Rivarola, Maximo Lisandro
Salvagiotti, Fernando
Faggioli, Valeria Soledad
author_role author
author2 Bacigaluppo, Silvina
Maury, Mariana
Ortiz, Jimena
Rivarola, Maximo Lisandro
Salvagiotti, Fernando
Faggioli, Valeria Soledad
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cultivos
Suelo
Aplicación de Abonos
Abonos Nitrogenados
Plantas de Cobertura
Intensificación
Crops
Soil
Fertilizer Application
Nitrogen Fertilizers
Cover Plants
Intensification
Fertilización Nitrogenada
Cultivos de Cobertura
Nitrogen Fertilization
topic Cultivos
Suelo
Aplicación de Abonos
Abonos Nitrogenados
Plantas de Cobertura
Intensificación
Crops
Soil
Fertilizer Application
Nitrogen Fertilizers
Cover Plants
Intensification
Fertilización Nitrogenada
Cultivos de Cobertura
Nitrogen Fertilization
dc.description.none.fl_txt_mv Introduction: The ecosystem services provided by soil microbial communities are critical for the resilience of agroecosystems, ensuring environmental conservation and food security. Long-term experiments comparing contrasting crop rotations are valuable tools for monitoring microbial responses, but they rarely include all crop phases within a single year. Therefore, the long-term agronomic impact may be masked by the immediate effect of the crop evaluated. In this study, we compared different crop rotations based on the Intensification Sequence Index (ISI), which considers the soil occupation time, and analyzed the impact of cover crops and two nitrogen fertilization strategies. Material and methods: We used an experiment initiated in 2006 with the following crop rotations: Soybean-Soybean, Maize-Wheat/Soybean, and Maize-Soybean-Wheat/Soybean. Soil samples were taken after the harvest of each summer crop phase (i.e., Soybean, Maize, Wheat/Soybean), and the soil prokaryotic community was monitored using 16S rRNA gene sequencing. Results and discussion: We observed that ISI and crop phase were the main predictors of microbial community composition, explaining 14.7% and 13.0% of the variation, respectively. Nitrogen fertilization had a minor effect (3.12%) and was detected only after maize sampling; cover crops had no significant effect. However, the presence of cover crops showed higher alpha diversity and an increased abundance of Proteobacteria. Maize enriched the abundance of certain taxa of Planctomycetes and Verrucomicrobia, while Soybean increased the abundance of Bacteroidetes and Proteobacteria. Comparatively, Soybean enriched Mucilaginibacter and Geobacter, while Wheat/Soybean enriched Brevundimonas and Roseimicrobium. Conclusion: Our results demonstrate that crop phase is as important as the long-term legacy of crop rotations in shaping the microbial community and that specific taxa responses are highly dependent on the crop phase surveyed.
EEA Marcos Juárez
Fil: Frene, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Bacigaluppo, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Oliveros; Argentina
Fil: Maury, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Ortiz, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rivarola, Maximo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salvagiotti, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Oliveros; Argentina
Fil: Faggioli, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
description Introduction: The ecosystem services provided by soil microbial communities are critical for the resilience of agroecosystems, ensuring environmental conservation and food security. Long-term experiments comparing contrasting crop rotations are valuable tools for monitoring microbial responses, but they rarely include all crop phases within a single year. Therefore, the long-term agronomic impact may be masked by the immediate effect of the crop evaluated. In this study, we compared different crop rotations based on the Intensification Sequence Index (ISI), which considers the soil occupation time, and analyzed the impact of cover crops and two nitrogen fertilization strategies. Material and methods: We used an experiment initiated in 2006 with the following crop rotations: Soybean-Soybean, Maize-Wheat/Soybean, and Maize-Soybean-Wheat/Soybean. Soil samples were taken after the harvest of each summer crop phase (i.e., Soybean, Maize, Wheat/Soybean), and the soil prokaryotic community was monitored using 16S rRNA gene sequencing. Results and discussion: We observed that ISI and crop phase were the main predictors of microbial community composition, explaining 14.7% and 13.0% of the variation, respectively. Nitrogen fertilization had a minor effect (3.12%) and was detected only after maize sampling; cover crops had no significant effect. However, the presence of cover crops showed higher alpha diversity and an increased abundance of Proteobacteria. Maize enriched the abundance of certain taxa of Planctomycetes and Verrucomicrobia, while Soybean increased the abundance of Bacteroidetes and Proteobacteria. Comparatively, Soybean enriched Mucilaginibacter and Geobacter, while Wheat/Soybean enriched Brevundimonas and Roseimicrobium. Conclusion: Our results demonstrate that crop phase is as important as the long-term legacy of crop rotations in shaping the microbial community and that specific taxa responses are highly dependent on the crop phase surveyed.
publishDate 2024
dc.date.none.fl_str_mv 2024-12
2025-06-13T13:08:34Z
2025-06-13T13:08:34Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22681
https://www.frontiersin.org/journals/agronomy/articles/10.3389/fagro.2024.1446404/full
2673-3218
https://doi.org/10.3389/fagro.2024.1446404
url http://hdl.handle.net/20.500.12123/22681
https://www.frontiersin.org/journals/agronomy/articles/10.3389/fagro.2024.1446404/full
https://doi.org/10.3389/fagro.2024.1446404
identifier_str_mv 2673-3218
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Frontiers in Agronomy 6 : 1446404. (December 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.000565

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