A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content
- Autores
- Soria, Mario; Soria, Maria Cecilia; Bueno, Dante Javier
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The present work compared 2 culture methods and a PCR assay applied with 2 enrichment methods for the detection of motile and nonmotile Salmonella strains using artificially contaminated egg content. The specificity (Sp) was 1 in all methods. The sensitivity (Se), accuracy (Ac), positive predictive value (PPV), and negative predictive value (NPV) were 1 in both culture methods for motile and nonmotile strains. In reference to the PCR methods, Se and PPV were between 0 and 1, whereas Ac and NPV were between 0.14 and 1. The detection level of motile and nonmotile strains was 5 to 54 cfu per 25 mL for both culture methods, but some strains could not be detected by the PCR methods. Extending incubation time of the enrichment medium to 5 d in the tetrathionate broth (TT), and Muller-Kauffmann tetrathionate-novobiocin broth (MKTTn) methods did not improve the isolation rates. All selective plating media did not show any statistical differences in the parameters of performance studied. Kappa coefficients showed that there was an excellent agreement between the bacteriological methods for all Salmonella strains. The agreement was very good and good between the PCR methods, for motile and nonmotile strains, respectively. However, there was a poor agreement when the PCR and bacteriological methods were compared for motile and nonmotile Salmonella strains. The TT and MKTTn methods are similar in terms of Ac, Se, Sp, PPV, and NPV for different Salmonella strains in egg content. The use of the PCR method cannot improve the same parameters, described before, in this matrix. So, further studies are needed to improve the performance parameters and limit of detection in egg content for the PCR methods, so that test can be used in poultry and food industry.
EEA Concepción del Uruguay
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina - Fuente
- Poultry Science 91 (10) : 2668–2676 (October 2012)
- Materia
-
Aves de Corral
Enfermedades de los Animales
Salmonella
PCR
Técnicas de Cultivo
Huevos
Poultry
Animal Diseases
Culture Techniques
Eggs - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/6467
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A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg contentSoria, MarioSoria, Maria CeciliaBueno, Dante JavierAves de CorralEnfermedades de los AnimalesSalmonellaPCRTécnicas de CultivoHuevosPoultryAnimal DiseasesCulture TechniquesEggsThe present work compared 2 culture methods and a PCR assay applied with 2 enrichment methods for the detection of motile and nonmotile Salmonella strains using artificially contaminated egg content. The specificity (Sp) was 1 in all methods. The sensitivity (Se), accuracy (Ac), positive predictive value (PPV), and negative predictive value (NPV) were 1 in both culture methods for motile and nonmotile strains. In reference to the PCR methods, Se and PPV were between 0 and 1, whereas Ac and NPV were between 0.14 and 1. The detection level of motile and nonmotile strains was 5 to 54 cfu per 25 mL for both culture methods, but some strains could not be detected by the PCR methods. Extending incubation time of the enrichment medium to 5 d in the tetrathionate broth (TT), and Muller-Kauffmann tetrathionate-novobiocin broth (MKTTn) methods did not improve the isolation rates. All selective plating media did not show any statistical differences in the parameters of performance studied. Kappa coefficients showed that there was an excellent agreement between the bacteriological methods for all Salmonella strains. The agreement was very good and good between the PCR methods, for motile and nonmotile strains, respectively. However, there was a poor agreement when the PCR and bacteriological methods were compared for motile and nonmotile Salmonella strains. The TT and MKTTn methods are similar in terms of Ac, Se, Sp, PPV, and NPV for different Salmonella strains in egg content. The use of the PCR method cannot improve the same parameters, described before, in this matrix. So, further studies are needed to improve the performance parameters and limit of detection in egg content for the PCR methods, so that test can be used in poultry and food industry.EEA Concepción del UruguayFil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaOxford Academic Press2019-12-06T14:32:44Z2019-12-06T14:32:44Z2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://academic.oup.com/ps/article/91/10/2668/1561306http://hdl.handle.net/20.500.12123/64670032-57911525-3171https://doi.org/10.3382/ps.2012-02253Poultry Science 91 (10) : 2668–2676 (October 2012)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:50Zoai:localhost:20.500.12123/6467instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:50.839INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content |
title |
A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content |
spellingShingle |
A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content Soria, Mario Aves de Corral Enfermedades de los Animales Salmonella PCR Técnicas de Cultivo Huevos Poultry Animal Diseases Culture Techniques Eggs |
title_short |
A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content |
title_full |
A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content |
title_fullStr |
A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content |
title_full_unstemmed |
A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content |
title_sort |
A comparative study of culture methods and polymerase chain reaction for Salmonella detection in egg content |
dc.creator.none.fl_str_mv |
Soria, Mario Soria, Maria Cecilia Bueno, Dante Javier |
author |
Soria, Mario |
author_facet |
Soria, Mario Soria, Maria Cecilia Bueno, Dante Javier |
author_role |
author |
author2 |
Soria, Maria Cecilia Bueno, Dante Javier |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Aves de Corral Enfermedades de los Animales Salmonella PCR Técnicas de Cultivo Huevos Poultry Animal Diseases Culture Techniques Eggs |
topic |
Aves de Corral Enfermedades de los Animales Salmonella PCR Técnicas de Cultivo Huevos Poultry Animal Diseases Culture Techniques Eggs |
dc.description.none.fl_txt_mv |
The present work compared 2 culture methods and a PCR assay applied with 2 enrichment methods for the detection of motile and nonmotile Salmonella strains using artificially contaminated egg content. The specificity (Sp) was 1 in all methods. The sensitivity (Se), accuracy (Ac), positive predictive value (PPV), and negative predictive value (NPV) were 1 in both culture methods for motile and nonmotile strains. In reference to the PCR methods, Se and PPV were between 0 and 1, whereas Ac and NPV were between 0.14 and 1. The detection level of motile and nonmotile strains was 5 to 54 cfu per 25 mL for both culture methods, but some strains could not be detected by the PCR methods. Extending incubation time of the enrichment medium to 5 d in the tetrathionate broth (TT), and Muller-Kauffmann tetrathionate-novobiocin broth (MKTTn) methods did not improve the isolation rates. All selective plating media did not show any statistical differences in the parameters of performance studied. Kappa coefficients showed that there was an excellent agreement between the bacteriological methods for all Salmonella strains. The agreement was very good and good between the PCR methods, for motile and nonmotile strains, respectively. However, there was a poor agreement when the PCR and bacteriological methods were compared for motile and nonmotile Salmonella strains. The TT and MKTTn methods are similar in terms of Ac, Se, Sp, PPV, and NPV for different Salmonella strains in egg content. The use of the PCR method cannot improve the same parameters, described before, in this matrix. So, further studies are needed to improve the performance parameters and limit of detection in egg content for the PCR methods, so that test can be used in poultry and food industry. EEA Concepción del Uruguay Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina |
description |
The present work compared 2 culture methods and a PCR assay applied with 2 enrichment methods for the detection of motile and nonmotile Salmonella strains using artificially contaminated egg content. The specificity (Sp) was 1 in all methods. The sensitivity (Se), accuracy (Ac), positive predictive value (PPV), and negative predictive value (NPV) were 1 in both culture methods for motile and nonmotile strains. In reference to the PCR methods, Se and PPV were between 0 and 1, whereas Ac and NPV were between 0.14 and 1. The detection level of motile and nonmotile strains was 5 to 54 cfu per 25 mL for both culture methods, but some strains could not be detected by the PCR methods. Extending incubation time of the enrichment medium to 5 d in the tetrathionate broth (TT), and Muller-Kauffmann tetrathionate-novobiocin broth (MKTTn) methods did not improve the isolation rates. All selective plating media did not show any statistical differences in the parameters of performance studied. Kappa coefficients showed that there was an excellent agreement between the bacteriological methods for all Salmonella strains. The agreement was very good and good between the PCR methods, for motile and nonmotile strains, respectively. However, there was a poor agreement when the PCR and bacteriological methods were compared for motile and nonmotile Salmonella strains. The TT and MKTTn methods are similar in terms of Ac, Se, Sp, PPV, and NPV for different Salmonella strains in egg content. The use of the PCR method cannot improve the same parameters, described before, in this matrix. So, further studies are needed to improve the performance parameters and limit of detection in egg content for the PCR methods, so that test can be used in poultry and food industry. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10 2019-12-06T14:32:44Z 2019-12-06T14:32:44Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://academic.oup.com/ps/article/91/10/2668/1561306 http://hdl.handle.net/20.500.12123/6467 0032-5791 1525-3171 https://doi.org/10.3382/ps.2012-02253 |
url |
https://academic.oup.com/ps/article/91/10/2668/1561306 http://hdl.handle.net/20.500.12123/6467 https://doi.org/10.3382/ps.2012-02253 |
identifier_str_mv |
0032-5791 1525-3171 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Oxford Academic Press |
publisher.none.fl_str_mv |
Oxford Academic Press |
dc.source.none.fl_str_mv |
Poultry Science 91 (10) : 2668–2676 (October 2012) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1844619140425318400 |
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12.559606 |