Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay

Autores
Félix, María L.; Muñoz-Leal, Sebastian; Carvalho, Luis A.; Queirolo, Diego; Remesar Alonso, Susana; Nava, Santiago; Armúa-Fernández, María T.; Venzal, José Manuel
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Ehrlichia are small intracellular Gram-negative bacteria transmitted by ticks. These microorganisms cause ehrlichiosis, a complex of life-threatening emerging zoonoses and diseases of global veterinary relevance. The aim of this study was to investigate the presence of Ehrlichia in free-living Ixodes auritulus collected in Uruguay. Ticks were collected from vegetation in five localities from the southeast and northeast of the country between 2014 and 2017. Detection of Ehrlichia DNA was performed in pools of adults or nymphs grouped according to the collection site and date. A total of 1,548 I. auritulus ticks were collected in four of the five locations sampled. Fragments of three loci (16S rRNA, dsb and groEL) were obtained by PCR, and phylogenies inferred using Bayesian inference analysis for each gene independently. DNA of Ehrlichia spp. was found in 15 out of 42 tick pools. Based on the topology of the phylogenetic trees, our sequences represent two novel genotypes for the genus named as Ehrlichia sp. Serrana and Ehrlichia sp. Laguna Negra. Both genotypes were closely related to Ehrlichia sp. Magellanica, a species detected in Ixodes uriae and Magellanic penguins. Considering that all stages of I. auritulus and I. uriae are parasites of birds, their phylogenetic relationships, and common eco-epidemiological profiles, it is reasonable to state that these genotypes of Ehrlichia spp. may represent a natural group likely associated with birds. Our results constitute the first characterization of Ehrlichia spp. in Uruguay. Future studies on birds reported as hosts for I. auritulus are needed to further understand the epidemiological cycles of both Ehrlichia genotypes in the country. Finally, I. auritulus does not feed on humans, so the two Ehrlichia species reported herein might have no implications in human health.
EEA Rafaela
Fil: Félix, María L. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
Fil: Muñoz-Leal, Sebastian. Universidad de Concepción. Facultad de Ciencias Veterinarias. Departamento de Patología y Medicina Preventiva; Chile
Fil: Carvalho, Luis A. AgResearch. Grasslands Research Centre; Nueva Zelanda
Fil: Queirolo, Diego. Universidad de La República. CENUR Noreste; Uruguay
Fil: Remesar Alonso, Susana. Universidade de Santiago de Compostela. Faculty of Veterinary Sciences. Department of Animal Pathology (INVESAGA Group); España
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Armúa-Fernández, María T. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
Fil: Venzal, José Manuel. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
Fuente
Current Research in Parasitology & Vector-Borne Diseases : 100022 (Available online 13 April 2021)
Materia
Ehrlichia
Genotipos
Ixodes
Uruguay
Marcadores Genéticos
Genotypes
Genetic Markers
Garrapatas
Ixodes auritulus
Marcadores Moleculares
Ticks
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9108
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9108
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from UruguayFélix, María L.Muñoz-Leal, SebastianCarvalho, Luis A.Queirolo, DiegoRemesar Alonso, SusanaNava, SantiagoArmúa-Fernández, María T.Venzal, José ManuelEhrlichiaGenotiposIxodesUruguayMarcadores GenéticosGenotypesGenetic MarkersGarrapatasIxodes auritulusMarcadores MolecularesTicksEhrlichia are small intracellular Gram-negative bacteria transmitted by ticks. These microorganisms cause ehrlichiosis, a complex of life-threatening emerging zoonoses and diseases of global veterinary relevance. The aim of this study was to investigate the presence of Ehrlichia in free-living Ixodes auritulus collected in Uruguay. Ticks were collected from vegetation in five localities from the southeast and northeast of the country between 2014 and 2017. Detection of Ehrlichia DNA was performed in pools of adults or nymphs grouped according to the collection site and date. A total of 1,548 I. auritulus ticks were collected in four of the five locations sampled. Fragments of three loci (16S rRNA, dsb and groEL) were obtained by PCR, and phylogenies inferred using Bayesian inference analysis for each gene independently. DNA of Ehrlichia spp. was found in 15 out of 42 tick pools. Based on the topology of the phylogenetic trees, our sequences represent two novel genotypes for the genus named as Ehrlichia sp. Serrana and Ehrlichia sp. Laguna Negra. Both genotypes were closely related to Ehrlichia sp. Magellanica, a species detected in Ixodes uriae and Magellanic penguins. Considering that all stages of I. auritulus and I. uriae are parasites of birds, their phylogenetic relationships, and common eco-epidemiological profiles, it is reasonable to state that these genotypes of Ehrlichia spp. may represent a natural group likely associated with birds. Our results constitute the first characterization of Ehrlichia spp. in Uruguay. Future studies on birds reported as hosts for I. auritulus are needed to further understand the epidemiological cycles of both Ehrlichia genotypes in the country. Finally, I. auritulus does not feed on humans, so the two Ehrlichia species reported herein might have no implications in human health.EEA RafaelaFil: Félix, María L. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; UruguayFil: Muñoz-Leal, Sebastian. Universidad de Concepción. Facultad de Ciencias Veterinarias. Departamento de Patología y Medicina Preventiva; ChileFil: Carvalho, Luis A. AgResearch. Grasslands Research Centre; Nueva ZelandaFil: Queirolo, Diego. Universidad de La República. CENUR Noreste; UruguayFil: Remesar Alonso, Susana. Universidade de Santiago de Compostela. Faculty of Veterinary Sciences. Department of Animal Pathology (INVESAGA Group); EspañaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Armúa-Fernández, María T. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; UruguayFil: Venzal, José Manuel. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; UruguayElsevier2021-04-16T13:33:04Z2021-04-16T13:33:04Z2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9108https://www.sciencedirect.com/science/article/pii/S2667114X210001692667-114Xhttps://doi.org/10.1016/j.crpvbd.2021.100022Current Research in Parasitology & Vector-Borne Diseases : 100022 (Available online 13 April 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:11Zoai:localhost:20.500.12123/9108instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:11.768INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
title Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
spellingShingle Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
Félix, María L.
Ehrlichia
Genotipos
Ixodes
Uruguay
Marcadores Genéticos
Genotypes
Genetic Markers
Garrapatas
Ixodes auritulus
Marcadores Moleculares
Ticks
title_short Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
title_full Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
title_fullStr Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
title_full_unstemmed Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
title_sort Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
dc.creator.none.fl_str_mv Félix, María L.
Muñoz-Leal, Sebastian
Carvalho, Luis A.
Queirolo, Diego
Remesar Alonso, Susana
Nava, Santiago
Armúa-Fernández, María T.
Venzal, José Manuel
author Félix, María L.
author_facet Félix, María L.
Muñoz-Leal, Sebastian
Carvalho, Luis A.
Queirolo, Diego
Remesar Alonso, Susana
Nava, Santiago
Armúa-Fernández, María T.
Venzal, José Manuel
author_role author
author2 Muñoz-Leal, Sebastian
Carvalho, Luis A.
Queirolo, Diego
Remesar Alonso, Susana
Nava, Santiago
Armúa-Fernández, María T.
Venzal, José Manuel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ehrlichia
Genotipos
Ixodes
Uruguay
Marcadores Genéticos
Genotypes
Genetic Markers
Garrapatas
Ixodes auritulus
Marcadores Moleculares
Ticks
topic Ehrlichia
Genotipos
Ixodes
Uruguay
Marcadores Genéticos
Genotypes
Genetic Markers
Garrapatas
Ixodes auritulus
Marcadores Moleculares
Ticks
dc.description.none.fl_txt_mv Ehrlichia are small intracellular Gram-negative bacteria transmitted by ticks. These microorganisms cause ehrlichiosis, a complex of life-threatening emerging zoonoses and diseases of global veterinary relevance. The aim of this study was to investigate the presence of Ehrlichia in free-living Ixodes auritulus collected in Uruguay. Ticks were collected from vegetation in five localities from the southeast and northeast of the country between 2014 and 2017. Detection of Ehrlichia DNA was performed in pools of adults or nymphs grouped according to the collection site and date. A total of 1,548 I. auritulus ticks were collected in four of the five locations sampled. Fragments of three loci (16S rRNA, dsb and groEL) were obtained by PCR, and phylogenies inferred using Bayesian inference analysis for each gene independently. DNA of Ehrlichia spp. was found in 15 out of 42 tick pools. Based on the topology of the phylogenetic trees, our sequences represent two novel genotypes for the genus named as Ehrlichia sp. Serrana and Ehrlichia sp. Laguna Negra. Both genotypes were closely related to Ehrlichia sp. Magellanica, a species detected in Ixodes uriae and Magellanic penguins. Considering that all stages of I. auritulus and I. uriae are parasites of birds, their phylogenetic relationships, and common eco-epidemiological profiles, it is reasonable to state that these genotypes of Ehrlichia spp. may represent a natural group likely associated with birds. Our results constitute the first characterization of Ehrlichia spp. in Uruguay. Future studies on birds reported as hosts for I. auritulus are needed to further understand the epidemiological cycles of both Ehrlichia genotypes in the country. Finally, I. auritulus does not feed on humans, so the two Ehrlichia species reported herein might have no implications in human health.
EEA Rafaela
Fil: Félix, María L. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
Fil: Muñoz-Leal, Sebastian. Universidad de Concepción. Facultad de Ciencias Veterinarias. Departamento de Patología y Medicina Preventiva; Chile
Fil: Carvalho, Luis A. AgResearch. Grasslands Research Centre; Nueva Zelanda
Fil: Queirolo, Diego. Universidad de La República. CENUR Noreste; Uruguay
Fil: Remesar Alonso, Susana. Universidade de Santiago de Compostela. Faculty of Veterinary Sciences. Department of Animal Pathology (INVESAGA Group); España
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Armúa-Fernández, María T. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
Fil: Venzal, José Manuel. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
description Ehrlichia are small intracellular Gram-negative bacteria transmitted by ticks. These microorganisms cause ehrlichiosis, a complex of life-threatening emerging zoonoses and diseases of global veterinary relevance. The aim of this study was to investigate the presence of Ehrlichia in free-living Ixodes auritulus collected in Uruguay. Ticks were collected from vegetation in five localities from the southeast and northeast of the country between 2014 and 2017. Detection of Ehrlichia DNA was performed in pools of adults or nymphs grouped according to the collection site and date. A total of 1,548 I. auritulus ticks were collected in four of the five locations sampled. Fragments of three loci (16S rRNA, dsb and groEL) were obtained by PCR, and phylogenies inferred using Bayesian inference analysis for each gene independently. DNA of Ehrlichia spp. was found in 15 out of 42 tick pools. Based on the topology of the phylogenetic trees, our sequences represent two novel genotypes for the genus named as Ehrlichia sp. Serrana and Ehrlichia sp. Laguna Negra. Both genotypes were closely related to Ehrlichia sp. Magellanica, a species detected in Ixodes uriae and Magellanic penguins. Considering that all stages of I. auritulus and I. uriae are parasites of birds, their phylogenetic relationships, and common eco-epidemiological profiles, it is reasonable to state that these genotypes of Ehrlichia spp. may represent a natural group likely associated with birds. Our results constitute the first characterization of Ehrlichia spp. in Uruguay. Future studies on birds reported as hosts for I. auritulus are needed to further understand the epidemiological cycles of both Ehrlichia genotypes in the country. Finally, I. auritulus does not feed on humans, so the two Ehrlichia species reported herein might have no implications in human health.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-16T13:33:04Z
2021-04-16T13:33:04Z
2021-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9108
https://www.sciencedirect.com/science/article/pii/S2667114X21000169
2667-114X
https://doi.org/10.1016/j.crpvbd.2021.100022
url http://hdl.handle.net/20.500.12123/9108
https://www.sciencedirect.com/science/article/pii/S2667114X21000169
https://doi.org/10.1016/j.crpvbd.2021.100022
identifier_str_mv 2667-114X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Current Research in Parasitology & Vector-Borne Diseases : 100022 (Available online 13 April 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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