Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay

Autores
Félix, María L.; Muñoz Leal, Sebastián; Carvalho, Luis A.; Queirolo, Diego; Remesar Alonso, Susana; Nava, Santiago; Armúa Fernández, María T.; Venzal, José M.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ehrlichia are small intracellular Gram-negative bacteria transmitted by ticks. These microorganisms cause ehrlichiosis, a complex of life-threatening emerging zoonoses and diseases of global veterinary relevance. The aim of this study was to investigate the presence of Ehrlichia in free-living Ixodes auritulus collected in Uruguay. Ticks were collected from vegetation in five localities from the southeast and northeast of the country between 2014 and 2017. Detection of Ehrlichia DNA was performed in pools of adults or nymphs grouped according to the collection site and date. A total of 1,548 I. auritulus ticks were collected in four of the five locations sampled. Fragments of three loci (16S rRNA, dsb and groEL) were obtained by PCR, and phylogenies inferred using Bayesian inference analysis for each gene independently. DNA of Ehrlichia spp. was found in 15 out of 42 tick pools. Based on the topology of the phylogenetic trees, our sequences represent two novel genotypes for the genus named as Ehrlichia sp. Serrana and Ehrlichia sp. Laguna Negra. Both genotypes were closely related to Ehrlichia sp. Magellanica, a species detected in Ixodes uriae and Magellanic penguins. Considering that all stages of I. auritulus and I. uriae are parasites of birds, their phylogenetic relationships, and common eco–epidemiological profiles, it is reasonable to state that these genotypes of Ehrlichia spp. may represent a natural group likely associated with birds. Our results constitute the first characterization of Ehrlichia spp. in Uruguay. Future studies on birds reported as hosts for I. auritulus are needed to further understand the epidemiological cycles of both Ehrlichia genotypes in the country. Finally, I. auritulus does not feed on humans, so the two Ehrlichia species reported herein might have no implications in human health.
Fil: Félix, María L.. Universidad de la Republica; Uruguay
Fil: Muñoz Leal, Sebastián. Universidad de Concepción; Chile
Fil: Carvalho, Luis A.. Grasslands Research Centre; Nueva Zelanda
Fil: Queirolo, Diego. Universidad de la Republica; Uruguay
Fil: Remesar Alonso, Susana. Universidad de Santiago de Compostela; España
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Armúa Fernández, María T.. Universidad de la Republica; Uruguay
Fil: Venzal, José M.. Universidad de la Republica; Uruguay
Materia
ANAPLASMATACEAE
BIRDS
EHRLICHIA
IXODES AURITULUS
NOVEL GENOTYPES
PHYLOGENY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184817
Acceder
id CONICETDig_328e3fd736eac3251c349bb0f5ec8643
oai_identifier_str oai:ri.conicet.gov.ar:11336/184817
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from UruguayFélix, María L.Muñoz Leal, SebastiánCarvalho, Luis A.Queirolo, DiegoRemesar Alonso, SusanaNava, SantiagoArmúa Fernández, María T.Venzal, José M.ANAPLASMATACEAEBIRDSEHRLICHIAIXODES AURITULUSNOVEL GENOTYPESPHYLOGENYhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Ehrlichia are small intracellular Gram-negative bacteria transmitted by ticks. These microorganisms cause ehrlichiosis, a complex of life-threatening emerging zoonoses and diseases of global veterinary relevance. The aim of this study was to investigate the presence of Ehrlichia in free-living Ixodes auritulus collected in Uruguay. Ticks were collected from vegetation in five localities from the southeast and northeast of the country between 2014 and 2017. Detection of Ehrlichia DNA was performed in pools of adults or nymphs grouped according to the collection site and date. A total of 1,548 I. auritulus ticks were collected in four of the five locations sampled. Fragments of three loci (16S rRNA, dsb and groEL) were obtained by PCR, and phylogenies inferred using Bayesian inference analysis for each gene independently. DNA of Ehrlichia spp. was found in 15 out of 42 tick pools. Based on the topology of the phylogenetic trees, our sequences represent two novel genotypes for the genus named as Ehrlichia sp. Serrana and Ehrlichia sp. Laguna Negra. Both genotypes were closely related to Ehrlichia sp. Magellanica, a species detected in Ixodes uriae and Magellanic penguins. Considering that all stages of I. auritulus and I. uriae are parasites of birds, their phylogenetic relationships, and common eco–epidemiological profiles, it is reasonable to state that these genotypes of Ehrlichia spp. may represent a natural group likely associated with birds. Our results constitute the first characterization of Ehrlichia spp. in Uruguay. Future studies on birds reported as hosts for I. auritulus are needed to further understand the epidemiological cycles of both Ehrlichia genotypes in the country. Finally, I. auritulus does not feed on humans, so the two Ehrlichia species reported herein might have no implications in human health.Fil: Félix, María L.. Universidad de la Republica; UruguayFil: Muñoz Leal, Sebastián. Universidad de Concepción; ChileFil: Carvalho, Luis A.. Grasslands Research Centre; Nueva ZelandaFil: Queirolo, Diego. Universidad de la Republica; UruguayFil: Remesar Alonso, Susana. Universidad de Santiago de Compostela; EspañaFil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Armúa Fernández, María T.. Universidad de la Republica; UruguayFil: Venzal, José M.. Universidad de la Republica; UruguayElsevier2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184817Félix, María L.; Muñoz Leal, Sebastián; Carvalho, Luis A.; Queirolo, Diego; Remesar Alonso, Susana; et al.; Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay; Elsevier; Current Research in Parasitology and Vector-Borne Diseases; 1; 1-2021; 1-72667-114XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2667114X21000169info:eu-repo/semantics/altIdentifier/doi/10.1016/j.crpvbd.2021.100022info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:27Zoai:ri.conicet.gov.ar:11336/184817instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:28.088CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
title Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
spellingShingle Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
Félix, María L.
ANAPLASMATACEAE
BIRDS
EHRLICHIA
IXODES AURITULUS
NOVEL GENOTYPES
PHYLOGENY
title_short Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
title_full Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
title_fullStr Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
title_full_unstemmed Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
title_sort Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
dc.creator.none.fl_str_mv Félix, María L.
Muñoz Leal, Sebastián
Carvalho, Luis A.
Queirolo, Diego
Remesar Alonso, Susana
Nava, Santiago
Armúa Fernández, María T.
Venzal, José M.
author Félix, María L.
author_facet Félix, María L.
Muñoz Leal, Sebastián
Carvalho, Luis A.
Queirolo, Diego
Remesar Alonso, Susana
Nava, Santiago
Armúa Fernández, María T.
Venzal, José M.
author_role author
author2 Muñoz Leal, Sebastián
Carvalho, Luis A.
Queirolo, Diego
Remesar Alonso, Susana
Nava, Santiago
Armúa Fernández, María T.
Venzal, José M.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANAPLASMATACEAE
BIRDS
EHRLICHIA
IXODES AURITULUS
NOVEL GENOTYPES
PHYLOGENY
topic ANAPLASMATACEAE
BIRDS
EHRLICHIA
IXODES AURITULUS
NOVEL GENOTYPES
PHYLOGENY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Ehrlichia are small intracellular Gram-negative bacteria transmitted by ticks. These microorganisms cause ehrlichiosis, a complex of life-threatening emerging zoonoses and diseases of global veterinary relevance. The aim of this study was to investigate the presence of Ehrlichia in free-living Ixodes auritulus collected in Uruguay. Ticks were collected from vegetation in five localities from the southeast and northeast of the country between 2014 and 2017. Detection of Ehrlichia DNA was performed in pools of adults or nymphs grouped according to the collection site and date. A total of 1,548 I. auritulus ticks were collected in four of the five locations sampled. Fragments of three loci (16S rRNA, dsb and groEL) were obtained by PCR, and phylogenies inferred using Bayesian inference analysis for each gene independently. DNA of Ehrlichia spp. was found in 15 out of 42 tick pools. Based on the topology of the phylogenetic trees, our sequences represent two novel genotypes for the genus named as Ehrlichia sp. Serrana and Ehrlichia sp. Laguna Negra. Both genotypes were closely related to Ehrlichia sp. Magellanica, a species detected in Ixodes uriae and Magellanic penguins. Considering that all stages of I. auritulus and I. uriae are parasites of birds, their phylogenetic relationships, and common eco–epidemiological profiles, it is reasonable to state that these genotypes of Ehrlichia spp. may represent a natural group likely associated with birds. Our results constitute the first characterization of Ehrlichia spp. in Uruguay. Future studies on birds reported as hosts for I. auritulus are needed to further understand the epidemiological cycles of both Ehrlichia genotypes in the country. Finally, I. auritulus does not feed on humans, so the two Ehrlichia species reported herein might have no implications in human health.
Fil: Félix, María L.. Universidad de la Republica; Uruguay
Fil: Muñoz Leal, Sebastián. Universidad de Concepción; Chile
Fil: Carvalho, Luis A.. Grasslands Research Centre; Nueva Zelanda
Fil: Queirolo, Diego. Universidad de la Republica; Uruguay
Fil: Remesar Alonso, Susana. Universidad de Santiago de Compostela; España
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Armúa Fernández, María T.. Universidad de la Republica; Uruguay
Fil: Venzal, José M.. Universidad de la Republica; Uruguay
description Ehrlichia are small intracellular Gram-negative bacteria transmitted by ticks. These microorganisms cause ehrlichiosis, a complex of life-threatening emerging zoonoses and diseases of global veterinary relevance. The aim of this study was to investigate the presence of Ehrlichia in free-living Ixodes auritulus collected in Uruguay. Ticks were collected from vegetation in five localities from the southeast and northeast of the country between 2014 and 2017. Detection of Ehrlichia DNA was performed in pools of adults or nymphs grouped according to the collection site and date. A total of 1,548 I. auritulus ticks were collected in four of the five locations sampled. Fragments of three loci (16S rRNA, dsb and groEL) were obtained by PCR, and phylogenies inferred using Bayesian inference analysis for each gene independently. DNA of Ehrlichia spp. was found in 15 out of 42 tick pools. Based on the topology of the phylogenetic trees, our sequences represent two novel genotypes for the genus named as Ehrlichia sp. Serrana and Ehrlichia sp. Laguna Negra. Both genotypes were closely related to Ehrlichia sp. Magellanica, a species detected in Ixodes uriae and Magellanic penguins. Considering that all stages of I. auritulus and I. uriae are parasites of birds, their phylogenetic relationships, and common eco–epidemiological profiles, it is reasonable to state that these genotypes of Ehrlichia spp. may represent a natural group likely associated with birds. Our results constitute the first characterization of Ehrlichia spp. in Uruguay. Future studies on birds reported as hosts for I. auritulus are needed to further understand the epidemiological cycles of both Ehrlichia genotypes in the country. Finally, I. auritulus does not feed on humans, so the two Ehrlichia species reported herein might have no implications in human health.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184817
Félix, María L.; Muñoz Leal, Sebastián; Carvalho, Luis A.; Queirolo, Diego; Remesar Alonso, Susana; et al.; Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay; Elsevier; Current Research in Parasitology and Vector-Borne Diseases; 1; 1-2021; 1-7
2667-114X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184817
identifier_str_mv Félix, María L.; Muñoz Leal, Sebastián; Carvalho, Luis A.; Queirolo, Diego; Remesar Alonso, Susana; et al.; Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay; Elsevier; Current Research in Parasitology and Vector-Borne Diseases; 1; 1-2021; 1-7
2667-114X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2667114X21000169
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.crpvbd.2021.100022
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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