Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina

Autores
Micheloud, Juan Francisco; Aguirre, Laura S.; Sandoval, Gabriela V.; Avellaneda Cáceres, Agustín; Diodati, Julian Angel; Peralta, Andrea Veronica
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine papular stomatitis virus (BPSV) is a parapoxvirus associated with papular and erosive lesions on the muzzle, lips, and oral mucosa of cattle. BPSV infection occurs worldwide; however, it has still not been unequivocally diagnosed. The present report describes an outbreak of BPSV infection affecting dairy calves in northwestern Argentina and provides the first molecular characterization of this virus in the country. The disease was detected in a dairy farm, affecting 33 calves between 2 and 20 days of age. The signs included reddish papules, ulcers, and scabby proliferative lesions on muzzle, lips, and oral mucosa. The affected calves resisted to being fed due to severe local pain. Two necropsies were performed; papulas and ulcers were observed in ruminal and omasal mucosa. Histologically, the affected areas of the skin showed acanthosis, spongiosis, and parakeratotic hyperkeratosis with adjacent focally extensive ulcers and multifocal inflammatory infiltrate in the epidermis. Eosinophilic inclusion bodies were detected in the cytoplasm of epithelial cells. DNA extracted from scab samples was analyzed by PCR using pan-parapoxvirus primers for the B2L gene. The sequence analysis revealed 99%, 85%, and 84% similarity with BPSV, Pseudocowpox virus, and Orf virus, respectively. A phylogenetic tree constructed using the B2L sequence showed that the virus clustered with BPSV isolates. Although clinical cases compatible with BSPV infection have been frequently described in Argentina, the present report is the first to identify the agent associated with cattle disease in the country.
Instituto de Investigación Animal Chaco Semiarido
Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal Chaco Semiarido. Grupo de Patología, Epidemiología e Investigación Diagnóstica-Área de Sanidad Animal; Argentina. Universidad Católica de Salta. Facultad de Ciencias Veterinarias. Cátedra Práctica Hospitalaria de Grandes Animales; Argentina
Fil: Aguirre, Laura S. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias. Cátedra Práctica Hospitalaria de Grandes Animales; Argentina
Fil: Sandoval, Gabriela V. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias. Cátedra Práctica Hospitalaria de Grandes Animales; Argentina
Fil: Avellaneda-Cáceres, Agustín. Universidad Católica de Salta. Cátedra de Práctica Hospitalaria de Grandes Animales; Argentina
Fil: Diodati, Julian Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina
Fil: Peralta, Andrea Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de investigaciones Científicas y Tecnológicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fuente
Tropical animal health and production : 1–7. (29 July 2019)
Materia
Cattle
Skin Diseases
Bovine Papular Stomatitis
Calves
Ganado Bovino
Parapoxvirus
Enfermedades de la Piel
Estomatitis Pustular Bovina
Argentina
Ternero
Bovine Papular Stomatitis Virus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5817
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5817
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network_name_str INTA Digital (INTA)
spelling Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in ArgentinaMicheloud, Juan FranciscoAguirre, Laura S.Sandoval, Gabriela V.Avellaneda Cáceres, AgustínDiodati, Julian AngelPeralta, Andrea VeronicaCattleSkin DiseasesBovine Papular StomatitisCalvesGanado BovinoParapoxvirusEnfermedades de la PielEstomatitis Pustular BovinaArgentinaTerneroBovine Papular Stomatitis VirusBovine papular stomatitis virus (BPSV) is a parapoxvirus associated with papular and erosive lesions on the muzzle, lips, and oral mucosa of cattle. BPSV infection occurs worldwide; however, it has still not been unequivocally diagnosed. The present report describes an outbreak of BPSV infection affecting dairy calves in northwestern Argentina and provides the first molecular characterization of this virus in the country. The disease was detected in a dairy farm, affecting 33 calves between 2 and 20 days of age. The signs included reddish papules, ulcers, and scabby proliferative lesions on muzzle, lips, and oral mucosa. The affected calves resisted to being fed due to severe local pain. Two necropsies were performed; papulas and ulcers were observed in ruminal and omasal mucosa. Histologically, the affected areas of the skin showed acanthosis, spongiosis, and parakeratotic hyperkeratosis with adjacent focally extensive ulcers and multifocal inflammatory infiltrate in the epidermis. Eosinophilic inclusion bodies were detected in the cytoplasm of epithelial cells. DNA extracted from scab samples was analyzed by PCR using pan-parapoxvirus primers for the B2L gene. The sequence analysis revealed 99%, 85%, and 84% similarity with BPSV, Pseudocowpox virus, and Orf virus, respectively. A phylogenetic tree constructed using the B2L sequence showed that the virus clustered with BPSV isolates. Although clinical cases compatible with BSPV infection have been frequently described in Argentina, the present report is the first to identify the agent associated with cattle disease in the country.Instituto de Investigación Animal Chaco SemiaridoFil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal Chaco Semiarido. Grupo de Patología, Epidemiología e Investigación Diagnóstica-Área de Sanidad Animal; Argentina. Universidad Católica de Salta. Facultad de Ciencias Veterinarias. Cátedra Práctica Hospitalaria de Grandes Animales; ArgentinaFil: Aguirre, Laura S. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias. Cátedra Práctica Hospitalaria de Grandes Animales; ArgentinaFil: Sandoval, Gabriela V. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias. Cátedra Práctica Hospitalaria de Grandes Animales; ArgentinaFil: Avellaneda-Cáceres, Agustín. Universidad Católica de Salta. Cátedra de Práctica Hospitalaria de Grandes Animales; ArgentinaFil: Diodati, Julian Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; ArgentinaFil: Peralta, Andrea Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de investigaciones Científicas y Tecnológicas. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaSpringer2019-09-09T12:28:43Z2019-09-09T12:28:43Z2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/5817https://link.springer.com/article/10.1007%2Fs11250-019-02006-w#citeas0049-47471573-7438 (Online)https://doi.org/10.1007/s11250-019-02006-wTropical animal health and production : 1–7. (29 July 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:17:03Zoai:localhost:20.500.12123/5817instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:03.802INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
title Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
spellingShingle Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
Micheloud, Juan Francisco
Cattle
Skin Diseases
Bovine Papular Stomatitis
Calves
Ganado Bovino
Parapoxvirus
Enfermedades de la Piel
Estomatitis Pustular Bovina
Argentina
Ternero
Bovine Papular Stomatitis Virus
title_short Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
title_full Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
title_fullStr Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
title_full_unstemmed Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
title_sort Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
dc.creator.none.fl_str_mv Micheloud, Juan Francisco
Aguirre, Laura S.
Sandoval, Gabriela V.
Avellaneda Cáceres, Agustín
Diodati, Julian Angel
Peralta, Andrea Veronica
author Micheloud, Juan Francisco
author_facet Micheloud, Juan Francisco
Aguirre, Laura S.
Sandoval, Gabriela V.
Avellaneda Cáceres, Agustín
Diodati, Julian Angel
Peralta, Andrea Veronica
author_role author
author2 Aguirre, Laura S.
Sandoval, Gabriela V.
Avellaneda Cáceres, Agustín
Diodati, Julian Angel
Peralta, Andrea Veronica
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cattle
Skin Diseases
Bovine Papular Stomatitis
Calves
Ganado Bovino
Parapoxvirus
Enfermedades de la Piel
Estomatitis Pustular Bovina
Argentina
Ternero
Bovine Papular Stomatitis Virus
topic Cattle
Skin Diseases
Bovine Papular Stomatitis
Calves
Ganado Bovino
Parapoxvirus
Enfermedades de la Piel
Estomatitis Pustular Bovina
Argentina
Ternero
Bovine Papular Stomatitis Virus
dc.description.none.fl_txt_mv Bovine papular stomatitis virus (BPSV) is a parapoxvirus associated with papular and erosive lesions on the muzzle, lips, and oral mucosa of cattle. BPSV infection occurs worldwide; however, it has still not been unequivocally diagnosed. The present report describes an outbreak of BPSV infection affecting dairy calves in northwestern Argentina and provides the first molecular characterization of this virus in the country. The disease was detected in a dairy farm, affecting 33 calves between 2 and 20 days of age. The signs included reddish papules, ulcers, and scabby proliferative lesions on muzzle, lips, and oral mucosa. The affected calves resisted to being fed due to severe local pain. Two necropsies were performed; papulas and ulcers were observed in ruminal and omasal mucosa. Histologically, the affected areas of the skin showed acanthosis, spongiosis, and parakeratotic hyperkeratosis with adjacent focally extensive ulcers and multifocal inflammatory infiltrate in the epidermis. Eosinophilic inclusion bodies were detected in the cytoplasm of epithelial cells. DNA extracted from scab samples was analyzed by PCR using pan-parapoxvirus primers for the B2L gene. The sequence analysis revealed 99%, 85%, and 84% similarity with BPSV, Pseudocowpox virus, and Orf virus, respectively. A phylogenetic tree constructed using the B2L sequence showed that the virus clustered with BPSV isolates. Although clinical cases compatible with BSPV infection have been frequently described in Argentina, the present report is the first to identify the agent associated with cattle disease in the country.
Instituto de Investigación Animal Chaco Semiarido
Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal Chaco Semiarido. Grupo de Patología, Epidemiología e Investigación Diagnóstica-Área de Sanidad Animal; Argentina. Universidad Católica de Salta. Facultad de Ciencias Veterinarias. Cátedra Práctica Hospitalaria de Grandes Animales; Argentina
Fil: Aguirre, Laura S. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias. Cátedra Práctica Hospitalaria de Grandes Animales; Argentina
Fil: Sandoval, Gabriela V. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias. Cátedra Práctica Hospitalaria de Grandes Animales; Argentina
Fil: Avellaneda-Cáceres, Agustín. Universidad Católica de Salta. Cátedra de Práctica Hospitalaria de Grandes Animales; Argentina
Fil: Diodati, Julian Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina
Fil: Peralta, Andrea Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de investigaciones Científicas y Tecnológicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina
description Bovine papular stomatitis virus (BPSV) is a parapoxvirus associated with papular and erosive lesions on the muzzle, lips, and oral mucosa of cattle. BPSV infection occurs worldwide; however, it has still not been unequivocally diagnosed. The present report describes an outbreak of BPSV infection affecting dairy calves in northwestern Argentina and provides the first molecular characterization of this virus in the country. The disease was detected in a dairy farm, affecting 33 calves between 2 and 20 days of age. The signs included reddish papules, ulcers, and scabby proliferative lesions on muzzle, lips, and oral mucosa. The affected calves resisted to being fed due to severe local pain. Two necropsies were performed; papulas and ulcers were observed in ruminal and omasal mucosa. Histologically, the affected areas of the skin showed acanthosis, spongiosis, and parakeratotic hyperkeratosis with adjacent focally extensive ulcers and multifocal inflammatory infiltrate in the epidermis. Eosinophilic inclusion bodies were detected in the cytoplasm of epithelial cells. DNA extracted from scab samples was analyzed by PCR using pan-parapoxvirus primers for the B2L gene. The sequence analysis revealed 99%, 85%, and 84% similarity with BPSV, Pseudocowpox virus, and Orf virus, respectively. A phylogenetic tree constructed using the B2L sequence showed that the virus clustered with BPSV isolates. Although clinical cases compatible with BSPV infection have been frequently described in Argentina, the present report is the first to identify the agent associated with cattle disease in the country.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-09T12:28:43Z
2019-09-09T12:28:43Z
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/5817
https://link.springer.com/article/10.1007%2Fs11250-019-02006-w#citeas
0049-4747
1573-7438 (Online)
https://doi.org/10.1007/s11250-019-02006-w
url http://hdl.handle.net/20.500.12123/5817
https://link.springer.com/article/10.1007%2Fs11250-019-02006-w#citeas
https://doi.org/10.1007/s11250-019-02006-w
identifier_str_mv 0049-4747
1573-7438 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Tropical animal health and production : 1–7. (29 July 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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