Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina

Autores
Micheloud, Juan Francisco; Aguirre Castro, Laura Sabrina; Sandoval, Gabriela Virginia; Avellaneda Caceres, Agustin; Diodati, Julian Angel; Peralta, Andrea Verónica
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine papular stomatitis virus (BPSV) is a parapoxvirus associated with papular and erosive lesions on the muzzle, lips, and oral mucosa of cattle. BPSV infection occurs worldwide; however, it has still not been unequivocally diagnosed. The present report describes an outbreak of BPSV infection affecting dairy calves in northwestern Argentina and provides the first molecular characterization of this virus in the country. The disease was detected in a dairy farm, affecting 33 calves between 2 and 20 days of age. The signs included reddish papules, ulcers, and scabby proliferative lesions on muzzle, lips, and oral mucosa. The affected calves resisted to being fed due to severe local pain. Two necropsies were performed; papulas and ulcers were observed in ruminal and omasal mucosa. Histologically, the affected areas of the skin showed acanthosis, spongiosis, and parakeratotic hyperkeratosis with adjacent focally extensive ulcers and multifocal inflammatory infiltrate in the epidermis. Eosinophilic inclusion bodies were detected in the cytoplasm of epithelial cells. DNA extracted from scab samples was analyzed by PCR using pan-parapoxvirus primers for the B2L gene. The sequence analysis revealed 99%, 85%, and 84% similarity with BPSV, Pseudocowpox virus, and Orf virus, respectively. A phylogenetic tree constructed using the B2L sequence showed that the virus clustered with BPSV isolates. Although clinical cases compatible with BSPV infection have been frequently described in Argentina, the present report is the first to identify the agent associated with cattle disease in the country.
Fil: Micheloud, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Aguirre Castro, Laura Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Salta; Argentina
Fil: Sandoval, Gabriela Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Salta; Argentina
Fil: Avellaneda Caceres, Agustin. Universidad Católica de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diodati, Julian Angel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Peralta, Andrea Verónica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Materia
BOVINE PAPULAR STOMATITIS VIRUS
CATTLE
CUTANEOUS DISEASE
PARAPOXVIRUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/147927
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/147927
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in ArgentinaMicheloud, Juan FranciscoAguirre Castro, Laura SabrinaSandoval, Gabriela VirginiaAvellaneda Caceres, AgustinDiodati, Julian AngelPeralta, Andrea VerónicaBOVINE PAPULAR STOMATITIS VIRUSCATTLECUTANEOUS DISEASEPARAPOXVIRUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bovine papular stomatitis virus (BPSV) is a parapoxvirus associated with papular and erosive lesions on the muzzle, lips, and oral mucosa of cattle. BPSV infection occurs worldwide; however, it has still not been unequivocally diagnosed. The present report describes an outbreak of BPSV infection affecting dairy calves in northwestern Argentina and provides the first molecular characterization of this virus in the country. The disease was detected in a dairy farm, affecting 33 calves between 2 and 20 days of age. The signs included reddish papules, ulcers, and scabby proliferative lesions on muzzle, lips, and oral mucosa. The affected calves resisted to being fed due to severe local pain. Two necropsies were performed; papulas and ulcers were observed in ruminal and omasal mucosa. Histologically, the affected areas of the skin showed acanthosis, spongiosis, and parakeratotic hyperkeratosis with adjacent focally extensive ulcers and multifocal inflammatory infiltrate in the epidermis. Eosinophilic inclusion bodies were detected in the cytoplasm of epithelial cells. DNA extracted from scab samples was analyzed by PCR using pan-parapoxvirus primers for the B2L gene. The sequence analysis revealed 99%, 85%, and 84% similarity with BPSV, Pseudocowpox virus, and Orf virus, respectively. A phylogenetic tree constructed using the B2L sequence showed that the virus clustered with BPSV isolates. Although clinical cases compatible with BSPV infection have been frequently described in Argentina, the present report is the first to identify the agent associated with cattle disease in the country.Fil: Micheloud, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Aguirre Castro, Laura Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Salta; ArgentinaFil: Sandoval, Gabriela Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Salta; ArgentinaFil: Avellaneda Caceres, Agustin. Universidad Católica de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Diodati, Julian Angel. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Peralta, Andrea Verónica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaSpringer2019-07-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/147927Micheloud, Juan Francisco; Aguirre Castro, Laura Sabrina; Sandoval, Gabriela Virginia; Avellaneda Caceres, Agustin; Diodati, Julian Angel; et al.; Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina; Springer; Tropical Animal Health And Production; 52; 1; 29-7-2019; 453-4590049-4747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11250-019-02006-winfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11250-019-02006-winfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:26:07Zoai:ri.conicet.gov.ar:11336/147927instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:26:08.132CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
title Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
spellingShingle Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
Micheloud, Juan Francisco
BOVINE PAPULAR STOMATITIS VIRUS
CATTLE
CUTANEOUS DISEASE
PARAPOXVIRUS
title_short Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
title_full Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
title_fullStr Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
title_full_unstemmed Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
title_sort Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina
dc.creator.none.fl_str_mv Micheloud, Juan Francisco
Aguirre Castro, Laura Sabrina
Sandoval, Gabriela Virginia
Avellaneda Caceres, Agustin
Diodati, Julian Angel
Peralta, Andrea Verónica
author Micheloud, Juan Francisco
author_facet Micheloud, Juan Francisco
Aguirre Castro, Laura Sabrina
Sandoval, Gabriela Virginia
Avellaneda Caceres, Agustin
Diodati, Julian Angel
Peralta, Andrea Verónica
author_role author
author2 Aguirre Castro, Laura Sabrina
Sandoval, Gabriela Virginia
Avellaneda Caceres, Agustin
Diodati, Julian Angel
Peralta, Andrea Verónica
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BOVINE PAPULAR STOMATITIS VIRUS
CATTLE
CUTANEOUS DISEASE
PARAPOXVIRUS
topic BOVINE PAPULAR STOMATITIS VIRUS
CATTLE
CUTANEOUS DISEASE
PARAPOXVIRUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bovine papular stomatitis virus (BPSV) is a parapoxvirus associated with papular and erosive lesions on the muzzle, lips, and oral mucosa of cattle. BPSV infection occurs worldwide; however, it has still not been unequivocally diagnosed. The present report describes an outbreak of BPSV infection affecting dairy calves in northwestern Argentina and provides the first molecular characterization of this virus in the country. The disease was detected in a dairy farm, affecting 33 calves between 2 and 20 days of age. The signs included reddish papules, ulcers, and scabby proliferative lesions on muzzle, lips, and oral mucosa. The affected calves resisted to being fed due to severe local pain. Two necropsies were performed; papulas and ulcers were observed in ruminal and omasal mucosa. Histologically, the affected areas of the skin showed acanthosis, spongiosis, and parakeratotic hyperkeratosis with adjacent focally extensive ulcers and multifocal inflammatory infiltrate in the epidermis. Eosinophilic inclusion bodies were detected in the cytoplasm of epithelial cells. DNA extracted from scab samples was analyzed by PCR using pan-parapoxvirus primers for the B2L gene. The sequence analysis revealed 99%, 85%, and 84% similarity with BPSV, Pseudocowpox virus, and Orf virus, respectively. A phylogenetic tree constructed using the B2L sequence showed that the virus clustered with BPSV isolates. Although clinical cases compatible with BSPV infection have been frequently described in Argentina, the present report is the first to identify the agent associated with cattle disease in the country.
Fil: Micheloud, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Aguirre Castro, Laura Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Salta; Argentina
Fil: Sandoval, Gabriela Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Salta; Argentina
Fil: Avellaneda Caceres, Agustin. Universidad Católica de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diodati, Julian Angel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Peralta, Andrea Verónica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
description Bovine papular stomatitis virus (BPSV) is a parapoxvirus associated with papular and erosive lesions on the muzzle, lips, and oral mucosa of cattle. BPSV infection occurs worldwide; however, it has still not been unequivocally diagnosed. The present report describes an outbreak of BPSV infection affecting dairy calves in northwestern Argentina and provides the first molecular characterization of this virus in the country. The disease was detected in a dairy farm, affecting 33 calves between 2 and 20 days of age. The signs included reddish papules, ulcers, and scabby proliferative lesions on muzzle, lips, and oral mucosa. The affected calves resisted to being fed due to severe local pain. Two necropsies were performed; papulas and ulcers were observed in ruminal and omasal mucosa. Histologically, the affected areas of the skin showed acanthosis, spongiosis, and parakeratotic hyperkeratosis with adjacent focally extensive ulcers and multifocal inflammatory infiltrate in the epidermis. Eosinophilic inclusion bodies were detected in the cytoplasm of epithelial cells. DNA extracted from scab samples was analyzed by PCR using pan-parapoxvirus primers for the B2L gene. The sequence analysis revealed 99%, 85%, and 84% similarity with BPSV, Pseudocowpox virus, and Orf virus, respectively. A phylogenetic tree constructed using the B2L sequence showed that the virus clustered with BPSV isolates. Although clinical cases compatible with BSPV infection have been frequently described in Argentina, the present report is the first to identify the agent associated with cattle disease in the country.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/147927
Micheloud, Juan Francisco; Aguirre Castro, Laura Sabrina; Sandoval, Gabriela Virginia; Avellaneda Caceres, Agustin; Diodati, Julian Angel; et al.; Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina; Springer; Tropical Animal Health And Production; 52; 1; 29-7-2019; 453-459
0049-4747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/147927
identifier_str_mv Micheloud, Juan Francisco; Aguirre Castro, Laura Sabrina; Sandoval, Gabriela Virginia; Avellaneda Caceres, Agustin; Diodati, Julian Angel; et al.; Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina; Springer; Tropical Animal Health And Production; 52; 1; 29-7-2019; 453-459
0049-4747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11250-019-02006-w
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11250-019-02006-w
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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