Cattle excreta on Lotus tenuis-promoted grasslands: Exploring soil microbiome and nitrous oxide emissions in the Salado River Basin, Argentina

Autores
Nieva, Amira Susana del Valle; Perez, Mónica Gabriela; Antonelli, Cristian Javier; Costantini, Alejandro Oscar; Ruiz, Oscar Adolfo
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cattle production generates inputs such as urine and dung that increase soil microbial diversity by adding organic matter and nitrogen. Legume pastures, such as Lotus tenuis improve soil quality by incorporating nitrogen, influencing microbial communities, and contributing to nitrous oxide (N2O) emissions. This study aimed to investigate the impact of livestock and pasture systems on soil microbiomes and N2O emissions within grassland ecosystems. We analysed microbial communities in soils of natural and L. tenuis-promoted grasslands, using Next Generation Sequencing (NGS) and simulated urine and dung depositions in field mesocosms. The results revealed significant differences in microbial diversity between natural and L. tenuis-promoted grasslands across cattle input treatments. Biomarker analysis identified distinct phyla in each type of excreta and grassland system. In natural grasslands, Proteobacteria and Actinobacteriota were prevalent with urine, whereas Acidobacteriota and Verrucomicrobiota characterised the soils promoted by L. tenuis. The indicators of the dung-treated soils were Pseudonocardia, Flavobacterium, NP-4, Adhaeribacter, Pseudoxanthomonas, and Novosphingobium, while Brevundimonas, Defluviicoccus, Sphingomonas, Sphingomicrobium, REEP01, Chthoniobacter, Hyphomicrobium, and PSRF01 were microbial indicators of the urine-treated soils. The N2O emissions were significantly lower in fields with L. tenuis and urine addition compared to natural grasslands under similar conditions, with genera like Brevundimonas, Hyphomicrobium, and Nitrosocosmicus positively correlated with emissions. These findings underscore the role of pasture composition in shaping soil microbiomes and highlight the benefits of legumes, such as L. tenuis, in reducing N2O emissions, providing an alternative for more sustainable livestock management practices.
Instituto de Suelos
Fil: Nieva, Amira Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Energía y Ambiente para el Desarrollo Sustentable; Argentina. Universidad Nacional de Catamarca. Centro Regional de Energía y Ambiente para el Desarrollo Sustentable; Argentina
Fil: Pérez, Mónica Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina
Fil: Antonelli, Cristian J. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de La Plata. Instituto de Fisiología Vegetal; Argentina
Fil: Costantini, Alejandro Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Catedra de Edafología; Argentina
Fil: Ruiz, Oscar. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnología; Argentina.
Fuente
European Journal of Soil Biology 128 : 103805. (March 2026)
Materia
Microbiomes
Greenhouse Gases
Urine
Livestock Manure
Cattle
Nitrous Oxide
Microbioma
Gases de Efecto Invernadero
Orina
Abono Animal
Lotus tenuis
Ganado Bovino
Óxido Nitroso
Río Salado, Buenos Aires
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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network_name_str INTA Digital (INTA)
spelling Cattle excreta on Lotus tenuis-promoted grasslands: Exploring soil microbiome and nitrous oxide emissions in the Salado River Basin, ArgentinaNieva, Amira Susana del VallePerez, Mónica GabrielaAntonelli, Cristian JavierCostantini, Alejandro OscarRuiz, Oscar AdolfoMicrobiomesGreenhouse GasesUrineLivestock ManureCattleNitrous OxideMicrobiomaGases de Efecto InvernaderoOrinaAbono AnimalLotus tenuisGanado BovinoÓxido NitrosoRío Salado, Buenos AiresCattle production generates inputs such as urine and dung that increase soil microbial diversity by adding organic matter and nitrogen. Legume pastures, such as Lotus tenuis improve soil quality by incorporating nitrogen, influencing microbial communities, and contributing to nitrous oxide (N2O) emissions. This study aimed to investigate the impact of livestock and pasture systems on soil microbiomes and N2O emissions within grassland ecosystems. We analysed microbial communities in soils of natural and L. tenuis-promoted grasslands, using Next Generation Sequencing (NGS) and simulated urine and dung depositions in field mesocosms. The results revealed significant differences in microbial diversity between natural and L. tenuis-promoted grasslands across cattle input treatments. Biomarker analysis identified distinct phyla in each type of excreta and grassland system. In natural grasslands, Proteobacteria and Actinobacteriota were prevalent with urine, whereas Acidobacteriota and Verrucomicrobiota characterised the soils promoted by L. tenuis. The indicators of the dung-treated soils were Pseudonocardia, Flavobacterium, NP-4, Adhaeribacter, Pseudoxanthomonas, and Novosphingobium, while Brevundimonas, Defluviicoccus, Sphingomonas, Sphingomicrobium, REEP01, Chthoniobacter, Hyphomicrobium, and PSRF01 were microbial indicators of the urine-treated soils. The N2O emissions were significantly lower in fields with L. tenuis and urine addition compared to natural grasslands under similar conditions, with genera like Brevundimonas, Hyphomicrobium, and Nitrosocosmicus positively correlated with emissions. These findings underscore the role of pasture composition in shaping soil microbiomes and highlight the benefits of legumes, such as L. tenuis, in reducing N2O emissions, providing an alternative for more sustainable livestock management practices.Instituto de SuelosFil: Nieva, Amira Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Energía y Ambiente para el Desarrollo Sustentable; Argentina. Universidad Nacional de Catamarca. Centro Regional de Energía y Ambiente para el Desarrollo Sustentable; ArgentinaFil: Pérez, Mónica Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; ArgentinaFil: Antonelli, Cristian J. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de La Plata. Instituto de Fisiología Vegetal; ArgentinaFil: Costantini, Alejandro Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Catedra de Edafología; ArgentinaFil: Ruiz, Oscar. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnología; Argentina.Elsevier2026-04-07T09:58:48Z2026-04-07T09:58:48Z2026-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25686https://www.sciencedirect.com/science/article/abs/pii/S11645563260000381164-55631778-3615https://doi.org/10.1016/j.ejsobi.2026.103805European Journal of Soil Biology 128 : 103805. (March 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-04-16T09:53:30Zoai:localhost:20.500.12123/25686instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-04-16 09:53:31.213INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Cattle excreta on Lotus tenuis-promoted grasslands: Exploring soil microbiome and nitrous oxide emissions in the Salado River Basin, Argentina
title Cattle excreta on Lotus tenuis-promoted grasslands: Exploring soil microbiome and nitrous oxide emissions in the Salado River Basin, Argentina
spellingShingle Cattle excreta on Lotus tenuis-promoted grasslands: Exploring soil microbiome and nitrous oxide emissions in the Salado River Basin, Argentina
Nieva, Amira Susana del Valle
Microbiomes
Greenhouse Gases
Urine
Livestock Manure
Cattle
Nitrous Oxide
Microbioma
Gases de Efecto Invernadero
Orina
Abono Animal
Lotus tenuis
Ganado Bovino
Óxido Nitroso
Río Salado, Buenos Aires
title_short Cattle excreta on Lotus tenuis-promoted grasslands: Exploring soil microbiome and nitrous oxide emissions in the Salado River Basin, Argentina
title_full Cattle excreta on Lotus tenuis-promoted grasslands: Exploring soil microbiome and nitrous oxide emissions in the Salado River Basin, Argentina
title_fullStr Cattle excreta on Lotus tenuis-promoted grasslands: Exploring soil microbiome and nitrous oxide emissions in the Salado River Basin, Argentina
title_full_unstemmed Cattle excreta on Lotus tenuis-promoted grasslands: Exploring soil microbiome and nitrous oxide emissions in the Salado River Basin, Argentina
title_sort Cattle excreta on Lotus tenuis-promoted grasslands: Exploring soil microbiome and nitrous oxide emissions in the Salado River Basin, Argentina
dc.creator.none.fl_str_mv Nieva, Amira Susana del Valle
Perez, Mónica Gabriela
Antonelli, Cristian Javier
Costantini, Alejandro Oscar
Ruiz, Oscar Adolfo
author Nieva, Amira Susana del Valle
author_facet Nieva, Amira Susana del Valle
Perez, Mónica Gabriela
Antonelli, Cristian Javier
Costantini, Alejandro Oscar
Ruiz, Oscar Adolfo
author_role author
author2 Perez, Mónica Gabriela
Antonelli, Cristian Javier
Costantini, Alejandro Oscar
Ruiz, Oscar Adolfo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Microbiomes
Greenhouse Gases
Urine
Livestock Manure
Cattle
Nitrous Oxide
Microbioma
Gases de Efecto Invernadero
Orina
Abono Animal
Lotus tenuis
Ganado Bovino
Óxido Nitroso
Río Salado, Buenos Aires
topic Microbiomes
Greenhouse Gases
Urine
Livestock Manure
Cattle
Nitrous Oxide
Microbioma
Gases de Efecto Invernadero
Orina
Abono Animal
Lotus tenuis
Ganado Bovino
Óxido Nitroso
Río Salado, Buenos Aires
dc.description.none.fl_txt_mv Cattle production generates inputs such as urine and dung that increase soil microbial diversity by adding organic matter and nitrogen. Legume pastures, such as Lotus tenuis improve soil quality by incorporating nitrogen, influencing microbial communities, and contributing to nitrous oxide (N2O) emissions. This study aimed to investigate the impact of livestock and pasture systems on soil microbiomes and N2O emissions within grassland ecosystems. We analysed microbial communities in soils of natural and L. tenuis-promoted grasslands, using Next Generation Sequencing (NGS) and simulated urine and dung depositions in field mesocosms. The results revealed significant differences in microbial diversity between natural and L. tenuis-promoted grasslands across cattle input treatments. Biomarker analysis identified distinct phyla in each type of excreta and grassland system. In natural grasslands, Proteobacteria and Actinobacteriota were prevalent with urine, whereas Acidobacteriota and Verrucomicrobiota characterised the soils promoted by L. tenuis. The indicators of the dung-treated soils were Pseudonocardia, Flavobacterium, NP-4, Adhaeribacter, Pseudoxanthomonas, and Novosphingobium, while Brevundimonas, Defluviicoccus, Sphingomonas, Sphingomicrobium, REEP01, Chthoniobacter, Hyphomicrobium, and PSRF01 were microbial indicators of the urine-treated soils. The N2O emissions were significantly lower in fields with L. tenuis and urine addition compared to natural grasslands under similar conditions, with genera like Brevundimonas, Hyphomicrobium, and Nitrosocosmicus positively correlated with emissions. These findings underscore the role of pasture composition in shaping soil microbiomes and highlight the benefits of legumes, such as L. tenuis, in reducing N2O emissions, providing an alternative for more sustainable livestock management practices.
Instituto de Suelos
Fil: Nieva, Amira Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Energía y Ambiente para el Desarrollo Sustentable; Argentina. Universidad Nacional de Catamarca. Centro Regional de Energía y Ambiente para el Desarrollo Sustentable; Argentina
Fil: Pérez, Mónica Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina
Fil: Antonelli, Cristian J. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de La Plata. Instituto de Fisiología Vegetal; Argentina
Fil: Costantini, Alejandro Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Catedra de Edafología; Argentina
Fil: Ruiz, Oscar. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnología; Argentina.
description Cattle production generates inputs such as urine and dung that increase soil microbial diversity by adding organic matter and nitrogen. Legume pastures, such as Lotus tenuis improve soil quality by incorporating nitrogen, influencing microbial communities, and contributing to nitrous oxide (N2O) emissions. This study aimed to investigate the impact of livestock and pasture systems on soil microbiomes and N2O emissions within grassland ecosystems. We analysed microbial communities in soils of natural and L. tenuis-promoted grasslands, using Next Generation Sequencing (NGS) and simulated urine and dung depositions in field mesocosms. The results revealed significant differences in microbial diversity between natural and L. tenuis-promoted grasslands across cattle input treatments. Biomarker analysis identified distinct phyla in each type of excreta and grassland system. In natural grasslands, Proteobacteria and Actinobacteriota were prevalent with urine, whereas Acidobacteriota and Verrucomicrobiota characterised the soils promoted by L. tenuis. The indicators of the dung-treated soils were Pseudonocardia, Flavobacterium, NP-4, Adhaeribacter, Pseudoxanthomonas, and Novosphingobium, while Brevundimonas, Defluviicoccus, Sphingomonas, Sphingomicrobium, REEP01, Chthoniobacter, Hyphomicrobium, and PSRF01 were microbial indicators of the urine-treated soils. The N2O emissions were significantly lower in fields with L. tenuis and urine addition compared to natural grasslands under similar conditions, with genera like Brevundimonas, Hyphomicrobium, and Nitrosocosmicus positively correlated with emissions. These findings underscore the role of pasture composition in shaping soil microbiomes and highlight the benefits of legumes, such as L. tenuis, in reducing N2O emissions, providing an alternative for more sustainable livestock management practices.
publishDate 2026
dc.date.none.fl_str_mv 2026-04-07T09:58:48Z
2026-04-07T09:58:48Z
2026-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25686
https://www.sciencedirect.com/science/article/abs/pii/S1164556326000038
1164-5563
1778-3615
https://doi.org/10.1016/j.ejsobi.2026.103805
url http://hdl.handle.net/20.500.12123/25686
https://www.sciencedirect.com/science/article/abs/pii/S1164556326000038
https://doi.org/10.1016/j.ejsobi.2026.103805
identifier_str_mv 1164-5563
1778-3615
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv European Journal of Soil Biology 128 : 103805. (March 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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