Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina

Autores
Lozano Calderon, Laura Camila; Cabanne, Gustavo Sebastián; Marcos, Andrea; Galdo Novo, Sabrina; Torres, Carolina; Perez, Andres; Pybus, Oliver G.; Konig, Guido Alberto
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Foot-and-mouth disease is a highly contagious disease affecting cloven-hoofed animals, resulting in considerable economic losses. Its causal agent is foot-and-mouth disease virus (FMDV), a picornavirus. Due to its error-prone replication and rapid evolution, the transmission and evolutionary dynamics of FMDV can be studied using genomic epidemiological approaches. To analyze FMDV evolution and identify possible transmission routes in an Argentinean region, field samples that tested positive for FMDV by PCR were obtained from 21 farms located in the Mar Chiquita district. Whole FMDV genome sequences were obtained by PCR amplification in seven fragments and sequencing using the Sanger technique. The genome sequences obtained from these samples were then analyzed using phylogenetic, phylogeographic, and evolutionary approaches. Three local transmission clusters were detected among the sampled viruses. The dataset was analyzed using Bayesian phylodynamic methods with appropriate coalescent and relaxed molecular clock models. The estimated mean viral evolutionary rate was 1.17 × 10− 2 substitutions/site/year. No significant differences in the rate of viral evolution were observed between farms with vaccinated animals and those with unvaccinated animals. The most recent common ancestor of the sampled sequences was dated to approximately one month before the first reported case in the outbreak. Virus transmission started in the south of the district and later dispersed to the west, and finally arrived in the east. Different transmission routes among the studied herds, such as non-replicating vectors and close contact contagion (i.e., aerosols), may be responsible for viral spread.
Instituto de Biotecnología
Fil: Lozano Calderon, Laura Camila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Lozano Calderon, Laura Camila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabanne, Gustavo Sebastián. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Cabanne, Gustavo Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marcos, Andrea. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Coordinación general de Epidemiología y Análisis de Riesgo; Argentina
Fil: Galdo Novo, Sabrina. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Dirección de Laboratorio Animal; Argentina
Fil: Torres, Carolina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM); Argentina
Fil: Perez, Andres. University of Minnesota. Department of Veterinary Population Medicine; Estados Unidos
Fil: Pybus, Oliver G. University of Oxford. Department of Biology; Reino Unido
Fil: Pybus, Oliver G. The Royal Veterinary College. Department of Pathobiology and Population Sciences; Reino Unido
Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Konig, Guido Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Archives of Virology 169 (5) : 101 (May 2024)
Materia
Aphthovirus
Phylogenetics
Phylogeography
Virus Fiebre Aftosa
Filogenética
Filogeografía
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/18362
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spelling Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, ArgentinaLozano Calderon, Laura CamilaCabanne, Gustavo SebastiánMarcos, AndreaGaldo Novo, SabrinaTorres, CarolinaPerez, AndresPybus, Oliver G.Konig, Guido AlbertoAphthovirusPhylogeneticsPhylogeographyVirus Fiebre AftosaFilogenéticaFilogeografíaFoot-and-mouth disease is a highly contagious disease affecting cloven-hoofed animals, resulting in considerable economic losses. Its causal agent is foot-and-mouth disease virus (FMDV), a picornavirus. Due to its error-prone replication and rapid evolution, the transmission and evolutionary dynamics of FMDV can be studied using genomic epidemiological approaches. To analyze FMDV evolution and identify possible transmission routes in an Argentinean region, field samples that tested positive for FMDV by PCR were obtained from 21 farms located in the Mar Chiquita district. Whole FMDV genome sequences were obtained by PCR amplification in seven fragments and sequencing using the Sanger technique. The genome sequences obtained from these samples were then analyzed using phylogenetic, phylogeographic, and evolutionary approaches. Three local transmission clusters were detected among the sampled viruses. The dataset was analyzed using Bayesian phylodynamic methods with appropriate coalescent and relaxed molecular clock models. The estimated mean viral evolutionary rate was 1.17 × 10− 2 substitutions/site/year. No significant differences in the rate of viral evolution were observed between farms with vaccinated animals and those with unvaccinated animals. The most recent common ancestor of the sampled sequences was dated to approximately one month before the first reported case in the outbreak. Virus transmission started in the south of the district and later dispersed to the west, and finally arrived in the east. Different transmission routes among the studied herds, such as non-replicating vectors and close contact contagion (i.e., aerosols), may be responsible for viral spread.Instituto de BiotecnologíaFil: Lozano Calderon, Laura Camila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Lozano Calderon, Laura Camila. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cabanne, Gustavo Sebastián. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Cabanne, Gustavo Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marcos, Andrea. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Coordinación general de Epidemiología y Análisis de Riesgo; ArgentinaFil: Galdo Novo, Sabrina. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Dirección de Laboratorio Animal; ArgentinaFil: Torres, Carolina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM); ArgentinaFil: Perez, Andres. University of Minnesota. Department of Veterinary Population Medicine; Estados UnidosFil: Pybus, Oliver G. University of Oxford. Department of Biology; Reino UnidoFil: Pybus, Oliver G. The Royal Veterinary College. Department of Pathobiology and Population Sciences; Reino UnidoFil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Konig, Guido Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2024-07-03T09:32:16Z2024-07-03T09:32:16Z2024-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18362https://link.springer.com/article/10.1007/s00705-024-06028-01432-8798https://doi.org/10.1007/s00705-024-06028-0Archives of Virology 169 (5) : 101 (May 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:37Zoai:localhost:20.500.12123/18362instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:38.129INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina
title Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina
spellingShingle Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina
Lozano Calderon, Laura Camila
Aphthovirus
Phylogenetics
Phylogeography
Virus Fiebre Aftosa
Filogenética
Filogeografía
title_short Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina
title_full Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina
title_fullStr Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina
title_full_unstemmed Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina
title_sort Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina
dc.creator.none.fl_str_mv Lozano Calderon, Laura Camila
Cabanne, Gustavo Sebastián
Marcos, Andrea
Galdo Novo, Sabrina
Torres, Carolina
Perez, Andres
Pybus, Oliver G.
Konig, Guido Alberto
author Lozano Calderon, Laura Camila
author_facet Lozano Calderon, Laura Camila
Cabanne, Gustavo Sebastián
Marcos, Andrea
Galdo Novo, Sabrina
Torres, Carolina
Perez, Andres
Pybus, Oliver G.
Konig, Guido Alberto
author_role author
author2 Cabanne, Gustavo Sebastián
Marcos, Andrea
Galdo Novo, Sabrina
Torres, Carolina
Perez, Andres
Pybus, Oliver G.
Konig, Guido Alberto
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Aphthovirus
Phylogenetics
Phylogeography
Virus Fiebre Aftosa
Filogenética
Filogeografía
topic Aphthovirus
Phylogenetics
Phylogeography
Virus Fiebre Aftosa
Filogenética
Filogeografía
dc.description.none.fl_txt_mv Foot-and-mouth disease is a highly contagious disease affecting cloven-hoofed animals, resulting in considerable economic losses. Its causal agent is foot-and-mouth disease virus (FMDV), a picornavirus. Due to its error-prone replication and rapid evolution, the transmission and evolutionary dynamics of FMDV can be studied using genomic epidemiological approaches. To analyze FMDV evolution and identify possible transmission routes in an Argentinean region, field samples that tested positive for FMDV by PCR were obtained from 21 farms located in the Mar Chiquita district. Whole FMDV genome sequences were obtained by PCR amplification in seven fragments and sequencing using the Sanger technique. The genome sequences obtained from these samples were then analyzed using phylogenetic, phylogeographic, and evolutionary approaches. Three local transmission clusters were detected among the sampled viruses. The dataset was analyzed using Bayesian phylodynamic methods with appropriate coalescent and relaxed molecular clock models. The estimated mean viral evolutionary rate was 1.17 × 10− 2 substitutions/site/year. No significant differences in the rate of viral evolution were observed between farms with vaccinated animals and those with unvaccinated animals. The most recent common ancestor of the sampled sequences was dated to approximately one month before the first reported case in the outbreak. Virus transmission started in the south of the district and later dispersed to the west, and finally arrived in the east. Different transmission routes among the studied herds, such as non-replicating vectors and close contact contagion (i.e., aerosols), may be responsible for viral spread.
Instituto de Biotecnología
Fil: Lozano Calderon, Laura Camila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Lozano Calderon, Laura Camila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabanne, Gustavo Sebastián. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Cabanne, Gustavo Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marcos, Andrea. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Coordinación general de Epidemiología y Análisis de Riesgo; Argentina
Fil: Galdo Novo, Sabrina. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Dirección de Laboratorio Animal; Argentina
Fil: Torres, Carolina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM); Argentina
Fil: Perez, Andres. University of Minnesota. Department of Veterinary Population Medicine; Estados Unidos
Fil: Pybus, Oliver G. University of Oxford. Department of Biology; Reino Unido
Fil: Pybus, Oliver G. The Royal Veterinary College. Department of Pathobiology and Population Sciences; Reino Unido
Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Konig, Guido Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Foot-and-mouth disease is a highly contagious disease affecting cloven-hoofed animals, resulting in considerable economic losses. Its causal agent is foot-and-mouth disease virus (FMDV), a picornavirus. Due to its error-prone replication and rapid evolution, the transmission and evolutionary dynamics of FMDV can be studied using genomic epidemiological approaches. To analyze FMDV evolution and identify possible transmission routes in an Argentinean region, field samples that tested positive for FMDV by PCR were obtained from 21 farms located in the Mar Chiquita district. Whole FMDV genome sequences were obtained by PCR amplification in seven fragments and sequencing using the Sanger technique. The genome sequences obtained from these samples were then analyzed using phylogenetic, phylogeographic, and evolutionary approaches. Three local transmission clusters were detected among the sampled viruses. The dataset was analyzed using Bayesian phylodynamic methods with appropriate coalescent and relaxed molecular clock models. The estimated mean viral evolutionary rate was 1.17 × 10− 2 substitutions/site/year. No significant differences in the rate of viral evolution were observed between farms with vaccinated animals and those with unvaccinated animals. The most recent common ancestor of the sampled sequences was dated to approximately one month before the first reported case in the outbreak. Virus transmission started in the south of the district and later dispersed to the west, and finally arrived in the east. Different transmission routes among the studied herds, such as non-replicating vectors and close contact contagion (i.e., aerosols), may be responsible for viral spread.
publishDate 2024
dc.date.none.fl_str_mv 2024-07-03T09:32:16Z
2024-07-03T09:32:16Z
2024-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18362
https://link.springer.com/article/10.1007/s00705-024-06028-0
1432-8798
https://doi.org/10.1007/s00705-024-06028-0
url http://hdl.handle.net/20.500.12123/18362
https://link.springer.com/article/10.1007/s00705-024-06028-0
https://doi.org/10.1007/s00705-024-06028-0
identifier_str_mv 1432-8798
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Archives of Virology 169 (5) : 101 (May 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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