Variability in spectrophotometric pyruvate analyses for predicting onion pungency and nutraceutical value
- Autores
- Beretta, Hebe Vanesa; Bannoud, Florencia; Insani, Ester Marina; Galmarini, Claudio Romulo; Cavagnaro, Pablo
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión aceptada
- Descripción
- Onion pyruvate concentration is used as a predictor of flavor intensity and nutraceutical value. The protocol of Schwimmer and Weston (SW) (1961) is the most widespread methodology for estimating onion pyruvate. Anthon and Barret (AB) (2003) proposed modifications to this procedure. Here, we compared these spectrophotometry-based procedures for pyruvate analysis using a diverse collection of onion cultivars. The SW method always led to over-estimation of pyruvate levels in colored, but not in white onions, by up to 65%. Identification of light-absorbance interfering compounds was performed by spectrophotometry and HPLC analysis. Interference by quercetin and anthocyanins, jointly, accounted for more than 90% of the over-estimation of pyruvate. Pyruvate determinations according to AB significantly reduced absorbance interference from compounds other than pyruvate. This study provides evidence about the mechanistic basis underlying differences between the SW and AB methods for indirect assessment of onion flavor and nutraceutical value.
Inst. de Biotecnología
Fil: Beretta, Hebe Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bannoud, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Insani, Ester Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Galmarini, Claudio Romulo. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Cavagnaro, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina - Fuente
- Food chemistry 224 : 201-206. (June 2017)
- Materia
-
Cebolla
Piruvatos
Espectrofotometria
Valor Nutritivo
Onions
Pyruvates
Spectrophotometry
Nutritive Value
Pungencia - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/1349
Ver los metadatos del registro completo
id |
INTADig_170dda67d7edeb0f84c6c7c29c221709 |
---|---|
oai_identifier_str |
oai:localhost:20.500.12123/1349 |
network_acronym_str |
INTADig |
repository_id_str |
l |
network_name_str |
INTA Digital (INTA) |
spelling |
Variability in spectrophotometric pyruvate analyses for predicting onion pungency and nutraceutical valueBeretta, Hebe VanesaBannoud, FlorenciaInsani, Ester MarinaGalmarini, Claudio RomuloCavagnaro, PabloCebollaPiruvatosEspectrofotometriaValor NutritivoOnionsPyruvatesSpectrophotometryNutritive ValuePungenciaOnion pyruvate concentration is used as a predictor of flavor intensity and nutraceutical value. The protocol of Schwimmer and Weston (SW) (1961) is the most widespread methodology for estimating onion pyruvate. Anthon and Barret (AB) (2003) proposed modifications to this procedure. Here, we compared these spectrophotometry-based procedures for pyruvate analysis using a diverse collection of onion cultivars. The SW method always led to over-estimation of pyruvate levels in colored, but not in white onions, by up to 65%. Identification of light-absorbance interfering compounds was performed by spectrophotometry and HPLC analysis. Interference by quercetin and anthocyanins, jointly, accounted for more than 90% of the over-estimation of pyruvate. Pyruvate determinations according to AB significantly reduced absorbance interference from compounds other than pyruvate. This study provides evidence about the mechanistic basis underlying differences between the SW and AB methods for indirect assessment of onion flavor and nutraceutical value.Inst. de BiotecnologíaFil: Beretta, Hebe Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bannoud, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Insani, Ester Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Galmarini, Claudio Romulo. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Cavagnaro, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina2017-09-28T14:42:18Z2017-09-28T14:42:18Z2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1349http://www.sciencedirect.com/science/article/pii/S03088146163204040308-8146https://doi.org/10.1016/j.foodchem.2016.12.031Food chemistry 224 : 201-206. (June 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:11Zoai:localhost:20.500.12123/1349instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:11.728INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Variability in spectrophotometric pyruvate analyses for predicting onion pungency and nutraceutical value |
title |
Variability in spectrophotometric pyruvate analyses for predicting onion pungency and nutraceutical value |
spellingShingle |
Variability in spectrophotometric pyruvate analyses for predicting onion pungency and nutraceutical value Beretta, Hebe Vanesa Cebolla Piruvatos Espectrofotometria Valor Nutritivo Onions Pyruvates Spectrophotometry Nutritive Value Pungencia |
title_short |
Variability in spectrophotometric pyruvate analyses for predicting onion pungency and nutraceutical value |
title_full |
Variability in spectrophotometric pyruvate analyses for predicting onion pungency and nutraceutical value |
title_fullStr |
Variability in spectrophotometric pyruvate analyses for predicting onion pungency and nutraceutical value |
title_full_unstemmed |
Variability in spectrophotometric pyruvate analyses for predicting onion pungency and nutraceutical value |
title_sort |
Variability in spectrophotometric pyruvate analyses for predicting onion pungency and nutraceutical value |
dc.creator.none.fl_str_mv |
Beretta, Hebe Vanesa Bannoud, Florencia Insani, Ester Marina Galmarini, Claudio Romulo Cavagnaro, Pablo |
author |
Beretta, Hebe Vanesa |
author_facet |
Beretta, Hebe Vanesa Bannoud, Florencia Insani, Ester Marina Galmarini, Claudio Romulo Cavagnaro, Pablo |
author_role |
author |
author2 |
Bannoud, Florencia Insani, Ester Marina Galmarini, Claudio Romulo Cavagnaro, Pablo |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Cebolla Piruvatos Espectrofotometria Valor Nutritivo Onions Pyruvates Spectrophotometry Nutritive Value Pungencia |
topic |
Cebolla Piruvatos Espectrofotometria Valor Nutritivo Onions Pyruvates Spectrophotometry Nutritive Value Pungencia |
dc.description.none.fl_txt_mv |
Onion pyruvate concentration is used as a predictor of flavor intensity and nutraceutical value. The protocol of Schwimmer and Weston (SW) (1961) is the most widespread methodology for estimating onion pyruvate. Anthon and Barret (AB) (2003) proposed modifications to this procedure. Here, we compared these spectrophotometry-based procedures for pyruvate analysis using a diverse collection of onion cultivars. The SW method always led to over-estimation of pyruvate levels in colored, but not in white onions, by up to 65%. Identification of light-absorbance interfering compounds was performed by spectrophotometry and HPLC analysis. Interference by quercetin and anthocyanins, jointly, accounted for more than 90% of the over-estimation of pyruvate. Pyruvate determinations according to AB significantly reduced absorbance interference from compounds other than pyruvate. This study provides evidence about the mechanistic basis underlying differences between the SW and AB methods for indirect assessment of onion flavor and nutraceutical value. Inst. de Biotecnología Fil: Beretta, Hebe Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bannoud, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Insani, Ester Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Galmarini, Claudio Romulo. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: Cavagnaro, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina |
description |
Onion pyruvate concentration is used as a predictor of flavor intensity and nutraceutical value. The protocol of Schwimmer and Weston (SW) (1961) is the most widespread methodology for estimating onion pyruvate. Anthon and Barret (AB) (2003) proposed modifications to this procedure. Here, we compared these spectrophotometry-based procedures for pyruvate analysis using a diverse collection of onion cultivars. The SW method always led to over-estimation of pyruvate levels in colored, but not in white onions, by up to 65%. Identification of light-absorbance interfering compounds was performed by spectrophotometry and HPLC analysis. Interference by quercetin and anthocyanins, jointly, accounted for more than 90% of the over-estimation of pyruvate. Pyruvate determinations according to AB significantly reduced absorbance interference from compounds other than pyruvate. This study provides evidence about the mechanistic basis underlying differences between the SW and AB methods for indirect assessment of onion flavor and nutraceutical value. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-28T14:42:18Z 2017-09-28T14:42:18Z 2017-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
acceptedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/1349 http://www.sciencedirect.com/science/article/pii/S0308814616320404 0308-8146 https://doi.org/10.1016/j.foodchem.2016.12.031 |
url |
http://hdl.handle.net/20.500.12123/1349 http://www.sciencedirect.com/science/article/pii/S0308814616320404 https://doi.org/10.1016/j.foodchem.2016.12.031 |
identifier_str_mv |
0308-8146 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Food chemistry 224 : 201-206. (June 2017) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
_version_ |
1844619117727842304 |
score |
12.559606 |