Over time variation in microsatellite (ssr) patterns in a natural wild potato population from northwest Argentina.

Autores
Leofanti, Gabriela; Camadro, Elsa Lucila; Erazzu, Luis Ernesto
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
The common potato, Solanum tuberosum L. (2n = 4x = 48), has 100–200 related wild taxonomic species endemic to the Americas, which constitute invaluable sources of genetic diversity. They form a polyploid series with 2n = 2x‐6x (x = 12), and can reproduce asexually by tubers and stolons and/or sexually by seeds. Information is scarce on their preponderant mode of reproduction, and its consequences on the genetic population structure over time. In previous work, a morphologically variable wild potato population from NW Argentina was sampled in two consecutive years. Two populations were ex situ regenerated from the sampled propagules (seeds in 2013 and tubers in 2014) for morphological and reproductive studies; these populations exhibited differences in reproductive behavior that could not be related to morphological phenotypes. To ascertain if the observed differences could be related to the captured genetic diversity in each year, a molecular analysis was carried out with seven microsatellite primer pairs located in seven chromosomes of the basic set. The captured genetic diversity, based on the proportion of individuals with a unique pattern of amplified fragments, varied significantly (χ2 α = 0.05) between sampling years. This could be possibly due to changes in the preponderant mode of reproduction, differential genotype fitness resulting from GxE interactions, or both. Wild potato accessions at germplasm banks are one‐time collection samples, thus, they may not be representative of the genetic diversity of the sampled population. To properly ex situ conserve the available genetic diversity, it is advisable to resample the sites whenever possible, and to conform each accession with the successive samples.
EEA Balcarce
Fil: Leofanti, Gabriela Agustina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Leofanti, Gabriela Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Leofanti, Gabriela Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Camadro, Elsa Lucila. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Camadro, Elsa Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Erazzú, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.
Fil: Erazzú, Luis Ernesto. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina.
Fuente
Crop Science (First published: 26 February 2021)
Materia
Solanum Tuberosum
Papa
Variación Genética
Germoplasma
Microsatélites
Argentina
Potatoes
Genetic Variation
Germplasm
Microsatellites
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8889
Acceder
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spelling Over time variation in microsatellite (ssr) patterns in a natural wild potato population from northwest Argentina.Leofanti, GabrielaCamadro, Elsa LucilaErazzu, Luis ErnestoSolanum TuberosumPapaVariación GenéticaGermoplasmaMicrosatélitesArgentinaPotatoesGenetic VariationGermplasmMicrosatellitesThe common potato, Solanum tuberosum L. (2n = 4x = 48), has 100–200 related wild taxonomic species endemic to the Americas, which constitute invaluable sources of genetic diversity. They form a polyploid series with 2n = 2x‐6x (x = 12), and can reproduce asexually by tubers and stolons and/or sexually by seeds. Information is scarce on their preponderant mode of reproduction, and its consequences on the genetic population structure over time. In previous work, a morphologically variable wild potato population from NW Argentina was sampled in two consecutive years. Two populations were ex situ regenerated from the sampled propagules (seeds in 2013 and tubers in 2014) for morphological and reproductive studies; these populations exhibited differences in reproductive behavior that could not be related to morphological phenotypes. To ascertain if the observed differences could be related to the captured genetic diversity in each year, a molecular analysis was carried out with seven microsatellite primer pairs located in seven chromosomes of the basic set. The captured genetic diversity, based on the proportion of individuals with a unique pattern of amplified fragments, varied significantly (χ2 α = 0.05) between sampling years. This could be possibly due to changes in the preponderant mode of reproduction, differential genotype fitness resulting from GxE interactions, or both. Wild potato accessions at germplasm banks are one‐time collection samples, thus, they may not be representative of the genetic diversity of the sampled population. To properly ex situ conserve the available genetic diversity, it is advisable to resample the sites whenever possible, and to conform each accession with the successive samples.EEA BalcarceFil: Leofanti, Gabriela Agustina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Leofanti, Gabriela Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Leofanti, Gabriela Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Camadro, Elsa Lucila. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Camadro, Elsa Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Erazzú, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.Fil: Erazzú, Luis Ernesto. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina.Crop Science Society of Americainfo:eu-repo/date/embargoEnd/2022-03-152021-03-15T11:38:22Z2021-03-15T11:38:22Z2021-02-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8889https://acsess.onlinelibrary.wiley.com/doi/10.1002/csc2.204981435-06530011-183Xhttps://doi.org/10.1002/csc2.20498Crop Science (First published: 26 February 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:48Zoai:localhost:20.500.12123/8889instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:48.776INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Over time variation in microsatellite (ssr) patterns in a natural wild potato population from northwest Argentina.
title Over time variation in microsatellite (ssr) patterns in a natural wild potato population from northwest Argentina.
spellingShingle Over time variation in microsatellite (ssr) patterns in a natural wild potato population from northwest Argentina.
Leofanti, Gabriela
Solanum Tuberosum
Papa
Variación Genética
Germoplasma
Microsatélites
Argentina
Potatoes
Genetic Variation
Germplasm
Microsatellites
title_short Over time variation in microsatellite (ssr) patterns in a natural wild potato population from northwest Argentina.
title_full Over time variation in microsatellite (ssr) patterns in a natural wild potato population from northwest Argentina.
title_fullStr Over time variation in microsatellite (ssr) patterns in a natural wild potato population from northwest Argentina.
title_full_unstemmed Over time variation in microsatellite (ssr) patterns in a natural wild potato population from northwest Argentina.
title_sort Over time variation in microsatellite (ssr) patterns in a natural wild potato population from northwest Argentina.
dc.creator.none.fl_str_mv Leofanti, Gabriela
Camadro, Elsa Lucila
Erazzu, Luis Ernesto
author Leofanti, Gabriela
author_facet Leofanti, Gabriela
Camadro, Elsa Lucila
Erazzu, Luis Ernesto
author_role author
author2 Camadro, Elsa Lucila
Erazzu, Luis Ernesto
author2_role author
author
dc.subject.none.fl_str_mv Solanum Tuberosum
Papa
Variación Genética
Germoplasma
Microsatélites
Argentina
Potatoes
Genetic Variation
Germplasm
Microsatellites
topic Solanum Tuberosum
Papa
Variación Genética
Germoplasma
Microsatélites
Argentina
Potatoes
Genetic Variation
Germplasm
Microsatellites
dc.description.none.fl_txt_mv The common potato, Solanum tuberosum L. (2n = 4x = 48), has 100–200 related wild taxonomic species endemic to the Americas, which constitute invaluable sources of genetic diversity. They form a polyploid series with 2n = 2x‐6x (x = 12), and can reproduce asexually by tubers and stolons and/or sexually by seeds. Information is scarce on their preponderant mode of reproduction, and its consequences on the genetic population structure over time. In previous work, a morphologically variable wild potato population from NW Argentina was sampled in two consecutive years. Two populations were ex situ regenerated from the sampled propagules (seeds in 2013 and tubers in 2014) for morphological and reproductive studies; these populations exhibited differences in reproductive behavior that could not be related to morphological phenotypes. To ascertain if the observed differences could be related to the captured genetic diversity in each year, a molecular analysis was carried out with seven microsatellite primer pairs located in seven chromosomes of the basic set. The captured genetic diversity, based on the proportion of individuals with a unique pattern of amplified fragments, varied significantly (χ2 α = 0.05) between sampling years. This could be possibly due to changes in the preponderant mode of reproduction, differential genotype fitness resulting from GxE interactions, or both. Wild potato accessions at germplasm banks are one‐time collection samples, thus, they may not be representative of the genetic diversity of the sampled population. To properly ex situ conserve the available genetic diversity, it is advisable to resample the sites whenever possible, and to conform each accession with the successive samples.
EEA Balcarce
Fil: Leofanti, Gabriela Agustina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Leofanti, Gabriela Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Leofanti, Gabriela Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Camadro, Elsa Lucila. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Camadro, Elsa Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Erazzú, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.
Fil: Erazzú, Luis Ernesto. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina.
description The common potato, Solanum tuberosum L. (2n = 4x = 48), has 100–200 related wild taxonomic species endemic to the Americas, which constitute invaluable sources of genetic diversity. They form a polyploid series with 2n = 2x‐6x (x = 12), and can reproduce asexually by tubers and stolons and/or sexually by seeds. Information is scarce on their preponderant mode of reproduction, and its consequences on the genetic population structure over time. In previous work, a morphologically variable wild potato population from NW Argentina was sampled in two consecutive years. Two populations were ex situ regenerated from the sampled propagules (seeds in 2013 and tubers in 2014) for morphological and reproductive studies; these populations exhibited differences in reproductive behavior that could not be related to morphological phenotypes. To ascertain if the observed differences could be related to the captured genetic diversity in each year, a molecular analysis was carried out with seven microsatellite primer pairs located in seven chromosomes of the basic set. The captured genetic diversity, based on the proportion of individuals with a unique pattern of amplified fragments, varied significantly (χ2 α = 0.05) between sampling years. This could be possibly due to changes in the preponderant mode of reproduction, differential genotype fitness resulting from GxE interactions, or both. Wild potato accessions at germplasm banks are one‐time collection samples, thus, they may not be representative of the genetic diversity of the sampled population. To properly ex situ conserve the available genetic diversity, it is advisable to resample the sites whenever possible, and to conform each accession with the successive samples.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-15T11:38:22Z
2021-03-15T11:38:22Z
2021-02-26
info:eu-repo/date/embargoEnd/2022-03-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8889
https://acsess.onlinelibrary.wiley.com/doi/10.1002/csc2.20498
1435-0653
0011-183X
https://doi.org/10.1002/csc2.20498
url http://hdl.handle.net/20.500.12123/8889
https://acsess.onlinelibrary.wiley.com/doi/10.1002/csc2.20498
https://doi.org/10.1002/csc2.20498
identifier_str_mv 1435-0653
0011-183X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Crop Science Society of America
publisher.none.fl_str_mv Crop Science Society of America
dc.source.none.fl_str_mv Crop Science (First published: 26 February 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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