Diversity structure of the microbial communities in the guts of four neotropical termite species

Autores
Vikram, Surendra; Arneodo Larochette, Joel Demián; Calcagno, Javier Ángel; Ortiz, Maximiliano Fabian; Mon, Maria Laura; Etcheverry, Clara; Cowan, Donald Arthur; Talia, Paola Monica
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The termite gut microbiome is dominated by lignocellulose degrading microorganisms. This study describes the intestinal microbiota of four Argentinian higher termite species with different feeding habits: Microcerotermes strunckii (hardwood), Nasutitermes corniger (softwood), Termes riograndensis (soil organic matter/grass) and Cornitermes cumulans (grass) by deep sequencing of amplified 16S rRNA and ITS genes. In addition, we have performed a taxonomic and gut community structure comparison incorporating into the analysis the previously reported microbiomes of additional termite species with varied diets. The bacterial phylum Spirochaetes was dominant in the guts of M. strunckii, N. corniger and C. cumulans, whereas Firmicutes predominated in the T. riograndensis gut microbiome. A single bacterial genus, Treponema (Spirochaetes), was dominant in all termite species, except for T. riograndensis. Both in our own sequenced samples and in the broader comparison, prokaryotic a-diversity was higher in the soil/grass feeders than in the wood feeders. Meanwhile, the β-diversity of prokaryotes and fungi was highly dissimilar among strict wood-feeders, whereas that of soil- and grass-feeders grouped more closely. Ascomycota and Basidiomycota were the only fungal phyla that could be identified in all gut samples, because of the lack of reference sequences in public databases. In summary, higher microbial diversity was recorded in termites with more versatile feeding sources, providing further evidence that diet, along with other factors (e.g., host taxonomy), influences the microbial community assembly in the termite gut.
Fil: Vikram, Surendra. University of Pretoria; Sudáfrica
Fil: Arneodo Larochette, Joel Demián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Calcagno, Javier Ángel. Universidad Maimonides. Centro de Ciencias Naturales, Ambientales y Antropologicas. Departamento de Ecologia y Ciencias Ambientales.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ortiz, Maximiliano Fabian. University of Pretoria; Sudáfrica
Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Etcheverry, Clara. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Biología. Cátedra Biología de los Invertebrados; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cowan, Donald Arthur. University of Pretoria; Sudáfrica
Fil: Talia, Paola Monica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Materia
GUT MICROBIOTA
ILLUMINA AMPLICON SEQUENCING
PROKARYOTIC AND FUNGAL DIVERSITY
TERMITE SPECIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/166363
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diversity structure of the microbial communities in the guts of four neotropical termite speciesVikram, SurendraArneodo Larochette, Joel DemiánCalcagno, Javier ÁngelOrtiz, Maximiliano FabianMon, Maria LauraEtcheverry, ClaraCowan, Donald ArthurTalia, Paola MonicaGUT MICROBIOTAILLUMINA AMPLICON SEQUENCINGPROKARYOTIC AND FUNGAL DIVERSITYTERMITE SPECIEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The termite gut microbiome is dominated by lignocellulose degrading microorganisms. This study describes the intestinal microbiota of four Argentinian higher termite species with different feeding habits: Microcerotermes strunckii (hardwood), Nasutitermes corniger (softwood), Termes riograndensis (soil organic matter/grass) and Cornitermes cumulans (grass) by deep sequencing of amplified 16S rRNA and ITS genes. In addition, we have performed a taxonomic and gut community structure comparison incorporating into the analysis the previously reported microbiomes of additional termite species with varied diets. The bacterial phylum Spirochaetes was dominant in the guts of M. strunckii, N. corniger and C. cumulans, whereas Firmicutes predominated in the T. riograndensis gut microbiome. A single bacterial genus, Treponema (Spirochaetes), was dominant in all termite species, except for T. riograndensis. Both in our own sequenced samples and in the broader comparison, prokaryotic a-diversity was higher in the soil/grass feeders than in the wood feeders. Meanwhile, the β-diversity of prokaryotes and fungi was highly dissimilar among strict wood-feeders, whereas that of soil- and grass-feeders grouped more closely. Ascomycota and Basidiomycota were the only fungal phyla that could be identified in all gut samples, because of the lack of reference sequences in public databases. In summary, higher microbial diversity was recorded in termites with more versatile feeding sources, providing further evidence that diet, along with other factors (e.g., host taxonomy), influences the microbial community assembly in the termite gut.Fil: Vikram, Surendra. University of Pretoria; SudáfricaFil: Arneodo Larochette, Joel Demián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Calcagno, Javier Ángel. Universidad Maimonides. Centro de Ciencias Naturales, Ambientales y Antropologicas. Departamento de Ecologia y Ciencias Ambientales.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ortiz, Maximiliano Fabian. University of Pretoria; SudáfricaFil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Etcheverry, Clara. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Biología. Cátedra Biología de los Invertebrados; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cowan, Donald Arthur. University of Pretoria; SudáfricaFil: Talia, Paola Monica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaPeerJ Inc.2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/166363Vikram, Surendra; Arneodo Larochette, Joel Demián; Calcagno, Javier Ángel; Ortiz, Maximiliano Fabian; Mon, Maria Laura; et al.; Diversity structure of the microbial communities in the guts of four neotropical termite species; PeerJ Inc.; PeerJ; 9; e10959; 4-2021; 1-232167-8359CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://peerj.com/articles/10959info:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.10959info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:36:09Zoai:ri.conicet.gov.ar:11336/166363instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:36:09.406CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diversity structure of the microbial communities in the guts of four neotropical termite species
title Diversity structure of the microbial communities in the guts of four neotropical termite species
spellingShingle Diversity structure of the microbial communities in the guts of four neotropical termite species
Vikram, Surendra
GUT MICROBIOTA
ILLUMINA AMPLICON SEQUENCING
PROKARYOTIC AND FUNGAL DIVERSITY
TERMITE SPECIES
title_short Diversity structure of the microbial communities in the guts of four neotropical termite species
title_full Diversity structure of the microbial communities in the guts of four neotropical termite species
title_fullStr Diversity structure of the microbial communities in the guts of four neotropical termite species
title_full_unstemmed Diversity structure of the microbial communities in the guts of four neotropical termite species
title_sort Diversity structure of the microbial communities in the guts of four neotropical termite species
dc.creator.none.fl_str_mv Vikram, Surendra
Arneodo Larochette, Joel Demián
Calcagno, Javier Ángel
Ortiz, Maximiliano Fabian
Mon, Maria Laura
Etcheverry, Clara
Cowan, Donald Arthur
Talia, Paola Monica
author Vikram, Surendra
author_facet Vikram, Surendra
Arneodo Larochette, Joel Demián
Calcagno, Javier Ángel
Ortiz, Maximiliano Fabian
Mon, Maria Laura
Etcheverry, Clara
Cowan, Donald Arthur
Talia, Paola Monica
author_role author
author2 Arneodo Larochette, Joel Demián
Calcagno, Javier Ángel
Ortiz, Maximiliano Fabian
Mon, Maria Laura
Etcheverry, Clara
Cowan, Donald Arthur
Talia, Paola Monica
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GUT MICROBIOTA
ILLUMINA AMPLICON SEQUENCING
PROKARYOTIC AND FUNGAL DIVERSITY
TERMITE SPECIES
topic GUT MICROBIOTA
ILLUMINA AMPLICON SEQUENCING
PROKARYOTIC AND FUNGAL DIVERSITY
TERMITE SPECIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The termite gut microbiome is dominated by lignocellulose degrading microorganisms. This study describes the intestinal microbiota of four Argentinian higher termite species with different feeding habits: Microcerotermes strunckii (hardwood), Nasutitermes corniger (softwood), Termes riograndensis (soil organic matter/grass) and Cornitermes cumulans (grass) by deep sequencing of amplified 16S rRNA and ITS genes. In addition, we have performed a taxonomic and gut community structure comparison incorporating into the analysis the previously reported microbiomes of additional termite species with varied diets. The bacterial phylum Spirochaetes was dominant in the guts of M. strunckii, N. corniger and C. cumulans, whereas Firmicutes predominated in the T. riograndensis gut microbiome. A single bacterial genus, Treponema (Spirochaetes), was dominant in all termite species, except for T. riograndensis. Both in our own sequenced samples and in the broader comparison, prokaryotic a-diversity was higher in the soil/grass feeders than in the wood feeders. Meanwhile, the β-diversity of prokaryotes and fungi was highly dissimilar among strict wood-feeders, whereas that of soil- and grass-feeders grouped more closely. Ascomycota and Basidiomycota were the only fungal phyla that could be identified in all gut samples, because of the lack of reference sequences in public databases. In summary, higher microbial diversity was recorded in termites with more versatile feeding sources, providing further evidence that diet, along with other factors (e.g., host taxonomy), influences the microbial community assembly in the termite gut.
Fil: Vikram, Surendra. University of Pretoria; Sudáfrica
Fil: Arneodo Larochette, Joel Demián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Calcagno, Javier Ángel. Universidad Maimonides. Centro de Ciencias Naturales, Ambientales y Antropologicas. Departamento de Ecologia y Ciencias Ambientales.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ortiz, Maximiliano Fabian. University of Pretoria; Sudáfrica
Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Etcheverry, Clara. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Biología. Cátedra Biología de los Invertebrados; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cowan, Donald Arthur. University of Pretoria; Sudáfrica
Fil: Talia, Paola Monica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
description The termite gut microbiome is dominated by lignocellulose degrading microorganisms. This study describes the intestinal microbiota of four Argentinian higher termite species with different feeding habits: Microcerotermes strunckii (hardwood), Nasutitermes corniger (softwood), Termes riograndensis (soil organic matter/grass) and Cornitermes cumulans (grass) by deep sequencing of amplified 16S rRNA and ITS genes. In addition, we have performed a taxonomic and gut community structure comparison incorporating into the analysis the previously reported microbiomes of additional termite species with varied diets. The bacterial phylum Spirochaetes was dominant in the guts of M. strunckii, N. corniger and C. cumulans, whereas Firmicutes predominated in the T. riograndensis gut microbiome. A single bacterial genus, Treponema (Spirochaetes), was dominant in all termite species, except for T. riograndensis. Both in our own sequenced samples and in the broader comparison, prokaryotic a-diversity was higher in the soil/grass feeders than in the wood feeders. Meanwhile, the β-diversity of prokaryotes and fungi was highly dissimilar among strict wood-feeders, whereas that of soil- and grass-feeders grouped more closely. Ascomycota and Basidiomycota were the only fungal phyla that could be identified in all gut samples, because of the lack of reference sequences in public databases. In summary, higher microbial diversity was recorded in termites with more versatile feeding sources, providing further evidence that diet, along with other factors (e.g., host taxonomy), influences the microbial community assembly in the termite gut.
publishDate 2021
dc.date.none.fl_str_mv 2021-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/166363
Vikram, Surendra; Arneodo Larochette, Joel Demián; Calcagno, Javier Ángel; Ortiz, Maximiliano Fabian; Mon, Maria Laura; et al.; Diversity structure of the microbial communities in the guts of four neotropical termite species; PeerJ Inc.; PeerJ; 9; e10959; 4-2021; 1-23
2167-8359
CONICET Digital
CONICET
url http://hdl.handle.net/11336/166363
identifier_str_mv Vikram, Surendra; Arneodo Larochette, Joel Demián; Calcagno, Javier Ángel; Ortiz, Maximiliano Fabian; Mon, Maria Laura; et al.; Diversity structure of the microbial communities in the guts of four neotropical termite species; PeerJ Inc.; PeerJ; 9; e10959; 4-2021; 1-23
2167-8359
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://peerj.com/articles/10959
info:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.10959
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv PeerJ Inc.
publisher.none.fl_str_mv PeerJ Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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