Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report.

Autores
Gual, Ignacio; Bartley, Paul; Katzer, Frank; Innes, Elisabeth; Canton, German Jose; Moore, Prando Dadin
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The work describes a case of Sarcocystis gigantea infection in a 3-years-old Corriedale ewe from Buenos Aires Province, Argentina. The ewe was found dead with a poor body condition. Pathological and molecular studies were carried out in order to try and confirm the causative agent of the infection. At necropsy, approx. 100 whitish elliptic (3–5 mm to 5–8 mm) macrocysts with a hard consistency were observed along the esophageal and pharyngeal muscular layers. Microscopically, the macrocysts consisted of an eosinophilic wall, internal septa originated from the eosinophilic wall and basophilic parasitic cells were located among the septa. The sarcocysts were identified molecularly through PCR amplification and sequencing of a short segment of the 18S rRNA gene. Sequence analysis of the amplified DNA demonstrated 100% identity to S. gigantea sequences previously published. To our knowledge this is the first molecular confirmation of S. gigantea infection in sheep in the Americas.
EEA Balcarce
Fil: Gual, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bartley, Paul. Moredum Research Institute Pentlands Science Park, Bush Loren, Penicuik, Edinburgh; Reino Unido
Fil: Katzer, Frank. Moredum Research Institute Pentlands Science Park, Bush Loren, Penicuik, Edinburgh; Reino Unido
Fil: Innes, Elisabeth. Moredum Research Institute Pentlands Science Park, Bush Loren, Penicuik, Edinburgh; Reino Unido
Fil: Cantón, Germán. INTA, Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Moore, Dadín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Veterinary parasitology 248 : 25-27. (15 December 2017)
Materia
Sarcocystis
Oveja
PCR
Argentina
Ewes
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5601
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oai_identifier_str oai:localhost:20.500.12123/5601
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network_name_str INTA Digital (INTA)
spelling Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report.Gual, IgnacioBartley, PaulKatzer, FrankInnes, ElisabethCanton, German JoseMoore, Prando DadinSarcocystisOvejaPCRArgentinaEwesThe work describes a case of Sarcocystis gigantea infection in a 3-years-old Corriedale ewe from Buenos Aires Province, Argentina. The ewe was found dead with a poor body condition. Pathological and molecular studies were carried out in order to try and confirm the causative agent of the infection. At necropsy, approx. 100 whitish elliptic (3–5 mm to 5–8 mm) macrocysts with a hard consistency were observed along the esophageal and pharyngeal muscular layers. Microscopically, the macrocysts consisted of an eosinophilic wall, internal septa originated from the eosinophilic wall and basophilic parasitic cells were located among the septa. The sarcocysts were identified molecularly through PCR amplification and sequencing of a short segment of the 18S rRNA gene. Sequence analysis of the amplified DNA demonstrated 100% identity to S. gigantea sequences previously published. To our knowledge this is the first molecular confirmation of S. gigantea infection in sheep in the Americas.EEA BalcarceFil: Gual, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bartley, Paul. Moredum Research Institute Pentlands Science Park, Bush Loren, Penicuik, Edinburgh; Reino UnidoFil: Katzer, Frank. Moredum Research Institute Pentlands Science Park, Bush Loren, Penicuik, Edinburgh; Reino UnidoFil: Innes, Elisabeth. Moredum Research Institute Pentlands Science Park, Bush Loren, Penicuik, Edinburgh; Reino UnidoFil: Cantón, Germán. INTA, Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Moore, Dadín. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-08-08T14:23:42Z2019-08-08T14:23:42Z2017-12-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0304401717304648?via%3Dihub#!http://hdl.handle.net/20.500.12123/56010304-4017https://doi.org/10.1016/j.vetpar.2017.10.017Veterinary parasitology 248 : 25-27. (15 December 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:43Zoai:localhost:20.500.12123/5601instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:44.228INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report.
title Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report.
spellingShingle Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report.
Gual, Ignacio
Sarcocystis
Oveja
PCR
Argentina
Ewes
title_short Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report.
title_full Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report.
title_fullStr Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report.
title_full_unstemmed Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report.
title_sort Molecular confirmation of sarcocystis gigantean in a naturally infected sheep in Argentina: a case report.
dc.creator.none.fl_str_mv Gual, Ignacio
Bartley, Paul
Katzer, Frank
Innes, Elisabeth
Canton, German Jose
Moore, Prando Dadin
author Gual, Ignacio
author_facet Gual, Ignacio
Bartley, Paul
Katzer, Frank
Innes, Elisabeth
Canton, German Jose
Moore, Prando Dadin
author_role author
author2 Bartley, Paul
Katzer, Frank
Innes, Elisabeth
Canton, German Jose
Moore, Prando Dadin
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Sarcocystis
Oveja
PCR
Argentina
Ewes
topic Sarcocystis
Oveja
PCR
Argentina
Ewes
dc.description.none.fl_txt_mv The work describes a case of Sarcocystis gigantea infection in a 3-years-old Corriedale ewe from Buenos Aires Province, Argentina. The ewe was found dead with a poor body condition. Pathological and molecular studies were carried out in order to try and confirm the causative agent of the infection. At necropsy, approx. 100 whitish elliptic (3–5 mm to 5–8 mm) macrocysts with a hard consistency were observed along the esophageal and pharyngeal muscular layers. Microscopically, the macrocysts consisted of an eosinophilic wall, internal septa originated from the eosinophilic wall and basophilic parasitic cells were located among the septa. The sarcocysts were identified molecularly through PCR amplification and sequencing of a short segment of the 18S rRNA gene. Sequence analysis of the amplified DNA demonstrated 100% identity to S. gigantea sequences previously published. To our knowledge this is the first molecular confirmation of S. gigantea infection in sheep in the Americas.
EEA Balcarce
Fil: Gual, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bartley, Paul. Moredum Research Institute Pentlands Science Park, Bush Loren, Penicuik, Edinburgh; Reino Unido
Fil: Katzer, Frank. Moredum Research Institute Pentlands Science Park, Bush Loren, Penicuik, Edinburgh; Reino Unido
Fil: Innes, Elisabeth. Moredum Research Institute Pentlands Science Park, Bush Loren, Penicuik, Edinburgh; Reino Unido
Fil: Cantón, Germán. INTA, Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Moore, Dadín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The work describes a case of Sarcocystis gigantea infection in a 3-years-old Corriedale ewe from Buenos Aires Province, Argentina. The ewe was found dead with a poor body condition. Pathological and molecular studies were carried out in order to try and confirm the causative agent of the infection. At necropsy, approx. 100 whitish elliptic (3–5 mm to 5–8 mm) macrocysts with a hard consistency were observed along the esophageal and pharyngeal muscular layers. Microscopically, the macrocysts consisted of an eosinophilic wall, internal septa originated from the eosinophilic wall and basophilic parasitic cells were located among the septa. The sarcocysts were identified molecularly through PCR amplification and sequencing of a short segment of the 18S rRNA gene. Sequence analysis of the amplified DNA demonstrated 100% identity to S. gigantea sequences previously published. To our knowledge this is the first molecular confirmation of S. gigantea infection in sheep in the Americas.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-15
2019-08-08T14:23:42Z
2019-08-08T14:23:42Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0304401717304648?via%3Dihub#!
http://hdl.handle.net/20.500.12123/5601
0304-4017
https://doi.org/10.1016/j.vetpar.2017.10.017
url https://www.sciencedirect.com/science/article/pii/S0304401717304648?via%3Dihub#!
http://hdl.handle.net/20.500.12123/5601
https://doi.org/10.1016/j.vetpar.2017.10.017
identifier_str_mv 0304-4017
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary parasitology 248 : 25-27. (15 December 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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