Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study

Autores
Andreoli, Valentina; Lopez, Sofia; Delgado, Santiago German; Perez, Sandra Elizabeth; Pereyra, Susana Beatriz; Gonzalez Altamiranda, Erika Analía; Romeo, Florencia; Grolli, Stefano; Verna, Andrea Elizabeth
Año de publicación
2025
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Reproductive viral diseases caused by Bovine alphaherpesvirus 1.1 (BoAHV-1.1), Bovine gam-maherpesvirus 4 (BoGHV-4), and Bovine viral diarrhoea virus (BVDV) impose a substantial economic burden on the cattle in dustry, primarily through infertility, abortion, and impaired reproductive performance. Owing to the limited efficacy of current antiviral strategies, this study evaluated the in vitro effects of platelet-rich plasma (PRP), at concentrations of 5 % and 10 %, on the replication kinetics of these viruses in Madin-Darby bovine kidney (MDBK) cells and primary bovine endometrial stromal cells (BESc). PRP modulated viral replication in a virus-, cell type-, and dose-related manner. In BoAHV-1.1-infected MDBK cells, 10 % PRP reduced extracellular titres but increases intracellular accumulation, suggesting interference with viral egress. In BESc, both intra- and extracellular titres decreased, consistent with a broader antiviral effect. For BoGHV-4 and BVDV, PRP induced variable and time-dependent responses across cell types. These results demonstrate that PRP can influence bovine viral replication dynamics in vitro and support further investigations into its mechanistic basis and in vivo therapeutic potential.
EEA Balcarce
Fil: Fil: Andreoli, Valentina. University of Parma. Department of Veterinary Medical Science; Italia
Fil: Lopez, Sofia. Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT); Argentina
Fil: Lopez, Sofia. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Delgado, Santiago German. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Perez, Sandra Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina
Fil: Perez, Sandra Elizabeth. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina
Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Gonzalez Altamiranda, Erika Analía. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Romeo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Grolli, Stefano. University of Parma. Department of Veterinary Medical Science; Italia
Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce, Argentina
Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fuente
Virus Research 361 : 199653 (November 2025)
Materia
Ganado Bovino
Virus
Herpes Virus Bovino
Plasma Sanguíneo
Inmunomodulación
Cattle
Viruses
Bovine Herpesvirus
Blood Plasma
Immunomodulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24998
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oai_identifier_str oai:localhost:20.500.12123/24998
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spelling Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept studyAndreoli, ValentinaLopez, SofiaDelgado, Santiago GermanPerez, Sandra ElizabethPereyra, Susana BeatrizGonzalez Altamiranda, Erika AnalíaRomeo, FlorenciaGrolli, StefanoVerna, Andrea ElizabethGanado BovinoVirusHerpes Virus BovinoPlasma SanguíneoInmunomodulaciónCattleVirusesBovine HerpesvirusBlood PlasmaImmunomodulationReproductive viral diseases caused by Bovine alphaherpesvirus 1.1 (BoAHV-1.1), Bovine gam-maherpesvirus 4 (BoGHV-4), and Bovine viral diarrhoea virus (BVDV) impose a substantial economic burden on the cattle in dustry, primarily through infertility, abortion, and impaired reproductive performance. Owing to the limited efficacy of current antiviral strategies, this study evaluated the in vitro effects of platelet-rich plasma (PRP), at concentrations of 5 % and 10 %, on the replication kinetics of these viruses in Madin-Darby bovine kidney (MDBK) cells and primary bovine endometrial stromal cells (BESc). PRP modulated viral replication in a virus-, cell type-, and dose-related manner. In BoAHV-1.1-infected MDBK cells, 10 % PRP reduced extracellular titres but increases intracellular accumulation, suggesting interference with viral egress. In BESc, both intra- and extracellular titres decreased, consistent with a broader antiviral effect. For BoGHV-4 and BVDV, PRP induced variable and time-dependent responses across cell types. These results demonstrate that PRP can influence bovine viral replication dynamics in vitro and support further investigations into its mechanistic basis and in vivo therapeutic potential.EEA BalcarceFil: Fil: Andreoli, Valentina. University of Parma. Department of Veterinary Medical Science; ItaliaFil: Lopez, Sofia. Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT); ArgentinaFil: Lopez, Sofia. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Delgado, Santiago German. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Perez, Sandra Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN); ArgentinaFil: Perez, Sandra Elizabeth. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Centro de Investigación Veterinaria de Tandil (CIVETAN); ArgentinaFil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Gonzalez Altamiranda, Erika Analía. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Romeo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Grolli, Stefano. University of Parma. Department of Veterinary Medical Science; ItaliaFil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce, ArgentinaFil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaElsevier2026-01-16T17:49:07Z2026-01-16T17:49:07Z2025-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/249980168-1702https://doi.org/10.1016/j.virusres.2025.199653Virus Research 361 : 199653 (November 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaspainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:47:41Zoai:localhost:20.500.12123/24998instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:47:41.441INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study
title Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study
spellingShingle Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study
Andreoli, Valentina
Ganado Bovino
Virus
Herpes Virus Bovino
Plasma Sanguíneo
Inmunomodulación
Cattle
Viruses
Bovine Herpesvirus
Blood Plasma
Immunomodulation
title_short Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study
title_full Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study
title_fullStr Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study
title_full_unstemmed Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study
title_sort Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study
dc.creator.none.fl_str_mv Andreoli, Valentina
Lopez, Sofia
Delgado, Santiago German
Perez, Sandra Elizabeth
Pereyra, Susana Beatriz
Gonzalez Altamiranda, Erika Analía
Romeo, Florencia
Grolli, Stefano
Verna, Andrea Elizabeth
author Andreoli, Valentina
author_facet Andreoli, Valentina
Lopez, Sofia
Delgado, Santiago German
Perez, Sandra Elizabeth
Pereyra, Susana Beatriz
Gonzalez Altamiranda, Erika Analía
Romeo, Florencia
Grolli, Stefano
Verna, Andrea Elizabeth
author_role author
author2 Lopez, Sofia
Delgado, Santiago German
Perez, Sandra Elizabeth
Pereyra, Susana Beatriz
Gonzalez Altamiranda, Erika Analía
Romeo, Florencia
Grolli, Stefano
Verna, Andrea Elizabeth
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Virus
Herpes Virus Bovino
Plasma Sanguíneo
Inmunomodulación
Cattle
Viruses
Bovine Herpesvirus
Blood Plasma
Immunomodulation
topic Ganado Bovino
Virus
Herpes Virus Bovino
Plasma Sanguíneo
Inmunomodulación
Cattle
Viruses
Bovine Herpesvirus
Blood Plasma
Immunomodulation
dc.description.none.fl_txt_mv Reproductive viral diseases caused by Bovine alphaherpesvirus 1.1 (BoAHV-1.1), Bovine gam-maherpesvirus 4 (BoGHV-4), and Bovine viral diarrhoea virus (BVDV) impose a substantial economic burden on the cattle in dustry, primarily through infertility, abortion, and impaired reproductive performance. Owing to the limited efficacy of current antiviral strategies, this study evaluated the in vitro effects of platelet-rich plasma (PRP), at concentrations of 5 % and 10 %, on the replication kinetics of these viruses in Madin-Darby bovine kidney (MDBK) cells and primary bovine endometrial stromal cells (BESc). PRP modulated viral replication in a virus-, cell type-, and dose-related manner. In BoAHV-1.1-infected MDBK cells, 10 % PRP reduced extracellular titres but increases intracellular accumulation, suggesting interference with viral egress. In BESc, both intra- and extracellular titres decreased, consistent with a broader antiviral effect. For BoGHV-4 and BVDV, PRP induced variable and time-dependent responses across cell types. These results demonstrate that PRP can influence bovine viral replication dynamics in vitro and support further investigations into its mechanistic basis and in vivo therapeutic potential.
EEA Balcarce
Fil: Fil: Andreoli, Valentina. University of Parma. Department of Veterinary Medical Science; Italia
Fil: Lopez, Sofia. Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT); Argentina
Fil: Lopez, Sofia. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Delgado, Santiago German. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Perez, Sandra Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina
Fil: Perez, Sandra Elizabeth. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina
Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Gonzalez Altamiranda, Erika Analía. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Romeo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Grolli, Stefano. University of Parma. Department of Veterinary Medical Science; Italia
Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce, Argentina
Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
description Reproductive viral diseases caused by Bovine alphaherpesvirus 1.1 (BoAHV-1.1), Bovine gam-maherpesvirus 4 (BoGHV-4), and Bovine viral diarrhoea virus (BVDV) impose a substantial economic burden on the cattle in dustry, primarily through infertility, abortion, and impaired reproductive performance. Owing to the limited efficacy of current antiviral strategies, this study evaluated the in vitro effects of platelet-rich plasma (PRP), at concentrations of 5 % and 10 %, on the replication kinetics of these viruses in Madin-Darby bovine kidney (MDBK) cells and primary bovine endometrial stromal cells (BESc). PRP modulated viral replication in a virus-, cell type-, and dose-related manner. In BoAHV-1.1-infected MDBK cells, 10 % PRP reduced extracellular titres but increases intracellular accumulation, suggesting interference with viral egress. In BESc, both intra- and extracellular titres decreased, consistent with a broader antiviral effect. For BoGHV-4 and BVDV, PRP induced variable and time-dependent responses across cell types. These results demonstrate that PRP can influence bovine viral replication dynamics in vitro and support further investigations into its mechanistic basis and in vivo therapeutic potential.
publishDate 2025
dc.date.none.fl_str_mv 2025-10
2026-01-16T17:49:07Z
2026-01-16T17:49:07Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24998
0168-1702
https://doi.org/10.1016/j.virusres.2025.199653
url http://hdl.handle.net/20.500.12123/24998
https://doi.org/10.1016/j.virusres.2025.199653
identifier_str_mv 0168-1702
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Virus Research 361 : 199653 (November 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.176822

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