Reactive oxygen species favors Varicellovirus bovinealpha 5 (BoAHV-5) replication in neural cells

Autores
Rosales, Juan José; Brunner, María Belén; Rodríguez, Marcelo; Marin, Maia Solange; Maldonado, Eduardo Néstor
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Varicellovirus bovinealpha (BoAHV) 1 and 5 are closely related neurotropic alphaherpesviruses with distinct neuropathogenic potential. BoAHV-5 causes meningoencephalitis in calves whereas encephalitis by BoAHV-1 infection is sporadic. the mechanisms underlying the differences in tropism and clinical outcomes of the infections are not yet completely understood. Here, we used neuroblastoma SH-SY5Y cells as non-differentiated in comparison with the SH-SY5Y neuronal-like cells obtained after exposing SH-SY5Y undifferentiated cells to trans-retinoic acid. We aimed to establish whether there was a relationship between the production of reactive oxygen species (ROS) and the kinetics of virus replication. We demonstrated that ROS production after BoAHV infection was higher in differentiated cells. Generation of ROS was also dependent on the infecting BoAHV strain. Higher ROS levels were produced during BoAHV-5 infection concomitantly with enhanced viral replication. We propose that increased ROS production mechanistically contributes to the tissue damage and neuroinflammation induced by BoAHV-5 infection. Future studies will determine specific targets of ROS that mediate the effects on viral replication.
EEA Balcarce
Fil: Rosales, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Brunner, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Rodríguez, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Marin, Maia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Maldonado, Eduardo Néstor. University of South Carolina, Estados Unidos
Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil; Argentina
Fuente
Mitochondrion 81 : 102005 (March 2025)
Materia
Virus
Especie de Oxígeno Reactivo
Células
Ganado Bovino
Viruses
Reactive Oxygen Species
Cells
Cattle
Varicellovirus bovinealpha
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22287
Acceder
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oai_identifier_str oai:localhost:20.500.12123/22287
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Reactive oxygen species favors Varicellovirus bovinealpha 5 (BoAHV-5) replication in neural cellsRosales, Juan JoséBrunner, María BelénRodríguez, MarceloMarin, Maia SolangeMaldonado, Eduardo NéstorVirusEspecie de Oxígeno ReactivoCélulasGanado BovinoVirusesReactive Oxygen SpeciesCellsCattleVaricellovirus bovinealphaVaricellovirus bovinealpha (BoAHV) 1 and 5 are closely related neurotropic alphaherpesviruses with distinct neuropathogenic potential. BoAHV-5 causes meningoencephalitis in calves whereas encephalitis by BoAHV-1 infection is sporadic. the mechanisms underlying the differences in tropism and clinical outcomes of the infections are not yet completely understood. Here, we used neuroblastoma SH-SY5Y cells as non-differentiated in comparison with the SH-SY5Y neuronal-like cells obtained after exposing SH-SY5Y undifferentiated cells to trans-retinoic acid. We aimed to establish whether there was a relationship between the production of reactive oxygen species (ROS) and the kinetics of virus replication. We demonstrated that ROS production after BoAHV infection was higher in differentiated cells. Generation of ROS was also dependent on the infecting BoAHV strain. Higher ROS levels were produced during BoAHV-5 infection concomitantly with enhanced viral replication. We propose that increased ROS production mechanistically contributes to the tissue damage and neuroinflammation induced by BoAHV-5 infection. Future studies will determine specific targets of ROS that mediate the effects on viral replication.EEA BalcarceFil: Rosales, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Brunner, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Rodríguez, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Marin, Maia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaFil: Maldonado, Eduardo Néstor. University of South Carolina, Estados UnidosFil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil; ArgentinaElsevierinfo:eu-repo/date/embargoEnd/2026-03-012025-05-15T10:33:35Z2025-05-15T10:33:35Z2025-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22287https://www.sciencedirect.com/science/article/abs/pii/S15677249250000291872-8278 (Online)1567-7249 (Print)https://doi.org/10.1016/j.mito.2025.102005Mitochondrion 81 : 102005 (March 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:51:03Zoai:localhost:20.500.12123/22287instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:51:04.072INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Reactive oxygen species favors Varicellovirus bovinealpha 5 (BoAHV-5) replication in neural cells
title Reactive oxygen species favors Varicellovirus bovinealpha 5 (BoAHV-5) replication in neural cells
spellingShingle Reactive oxygen species favors Varicellovirus bovinealpha 5 (BoAHV-5) replication in neural cells
Rosales, Juan José
Virus
Especie de Oxígeno Reactivo
Células
Ganado Bovino
Viruses
Reactive Oxygen Species
Cells
Cattle
Varicellovirus bovinealpha
title_short Reactive oxygen species favors Varicellovirus bovinealpha 5 (BoAHV-5) replication in neural cells
title_full Reactive oxygen species favors Varicellovirus bovinealpha 5 (BoAHV-5) replication in neural cells
title_fullStr Reactive oxygen species favors Varicellovirus bovinealpha 5 (BoAHV-5) replication in neural cells
title_full_unstemmed Reactive oxygen species favors Varicellovirus bovinealpha 5 (BoAHV-5) replication in neural cells
title_sort Reactive oxygen species favors Varicellovirus bovinealpha 5 (BoAHV-5) replication in neural cells
dc.creator.none.fl_str_mv Rosales, Juan José
Brunner, María Belén
Rodríguez, Marcelo
Marin, Maia Solange
Maldonado, Eduardo Néstor
author Rosales, Juan José
author_facet Rosales, Juan José
Brunner, María Belén
Rodríguez, Marcelo
Marin, Maia Solange
Maldonado, Eduardo Néstor
author_role author
author2 Brunner, María Belén
Rodríguez, Marcelo
Marin, Maia Solange
Maldonado, Eduardo Néstor
author2_role author
author
author
author
dc.subject.none.fl_str_mv Virus
Especie de Oxígeno Reactivo
Células
Ganado Bovino
Viruses
Reactive Oxygen Species
Cells
Cattle
Varicellovirus bovinealpha
topic Virus
Especie de Oxígeno Reactivo
Células
Ganado Bovino
Viruses
Reactive Oxygen Species
Cells
Cattle
Varicellovirus bovinealpha
dc.description.none.fl_txt_mv Varicellovirus bovinealpha (BoAHV) 1 and 5 are closely related neurotropic alphaherpesviruses with distinct neuropathogenic potential. BoAHV-5 causes meningoencephalitis in calves whereas encephalitis by BoAHV-1 infection is sporadic. the mechanisms underlying the differences in tropism and clinical outcomes of the infections are not yet completely understood. Here, we used neuroblastoma SH-SY5Y cells as non-differentiated in comparison with the SH-SY5Y neuronal-like cells obtained after exposing SH-SY5Y undifferentiated cells to trans-retinoic acid. We aimed to establish whether there was a relationship between the production of reactive oxygen species (ROS) and the kinetics of virus replication. We demonstrated that ROS production after BoAHV infection was higher in differentiated cells. Generation of ROS was also dependent on the infecting BoAHV strain. Higher ROS levels were produced during BoAHV-5 infection concomitantly with enhanced viral replication. We propose that increased ROS production mechanistically contributes to the tissue damage and neuroinflammation induced by BoAHV-5 infection. Future studies will determine specific targets of ROS that mediate the effects on viral replication.
EEA Balcarce
Fil: Rosales, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Brunner, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Rodríguez, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Marin, Maia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Maldonado, Eduardo Néstor. University of South Carolina, Estados Unidos
Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil; Argentina
description Varicellovirus bovinealpha (BoAHV) 1 and 5 are closely related neurotropic alphaherpesviruses with distinct neuropathogenic potential. BoAHV-5 causes meningoencephalitis in calves whereas encephalitis by BoAHV-1 infection is sporadic. the mechanisms underlying the differences in tropism and clinical outcomes of the infections are not yet completely understood. Here, we used neuroblastoma SH-SY5Y cells as non-differentiated in comparison with the SH-SY5Y neuronal-like cells obtained after exposing SH-SY5Y undifferentiated cells to trans-retinoic acid. We aimed to establish whether there was a relationship between the production of reactive oxygen species (ROS) and the kinetics of virus replication. We demonstrated that ROS production after BoAHV infection was higher in differentiated cells. Generation of ROS was also dependent on the infecting BoAHV strain. Higher ROS levels were produced during BoAHV-5 infection concomitantly with enhanced viral replication. We propose that increased ROS production mechanistically contributes to the tissue damage and neuroinflammation induced by BoAHV-5 infection. Future studies will determine specific targets of ROS that mediate the effects on viral replication.
publishDate 2025
dc.date.none.fl_str_mv 2025-05-15T10:33:35Z
2025-05-15T10:33:35Z
2025-03
info:eu-repo/date/embargoEnd/2026-03-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22287
https://www.sciencedirect.com/science/article/abs/pii/S1567724925000029
1872-8278 (Online)
1567-7249 (Print)
https://doi.org/10.1016/j.mito.2025.102005
url http://hdl.handle.net/20.500.12123/22287
https://www.sciencedirect.com/science/article/abs/pii/S1567724925000029
https://doi.org/10.1016/j.mito.2025.102005
identifier_str_mv 1872-8278 (Online)
1567-7249 (Print)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Mitochondrion 81 : 102005 (March 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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