Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants

Autores
Raschia, Maria Agustina; Caffaro, María Eugenia; Rossi, Ursula Amaranta; Poli, Mario Andres
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A growing interest in the production and commercialization of A2 cow's milk has been observed in many countries in the last few years due to the beneficial properties for human health attributed to A2 β-casein variant. Methods of varying complexity and different equipment requirements have been proposed for the determination of the β-casein genotype of individual cows. We proposed herein a modification of a previously patented method based on an amplification-created restriction site PCR followed by restriction fragment length polymorphism analysis. This method allows to identify and differentiate A2-like from A1-like β-casein variants, after differential endonuclease cleavage flanking the nucleotide that determines the amino acid at position 67 of β-casein. The advantages of this method are that it: • enables to unequivocally score A2-like as well as A1-like β-casein variants, • can be performed at low cost in simply equipped molecular biology laboratories, and • can be scaled up to analyze hundreds of samples per day. For these reasons, and based on the results obtained from the analysis carried out in this work, it showed to be a reliable method for the screening of herds to selective breeding of homozygous cows and bulls for A2 or A2-like alleles.
Instituto de Genética
Fil: Raschia, Maria Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Rossi, Ursula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Rossi, Ursula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fuente
MethodsX 10 : 102183. (April 2023)
Materia
Ganado Bovino
Métodos
Caseína
Beta-caseína
Leche de Vaca
Cattle
Methods
Casein
Beta-casein
Cow Milk
PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14567
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oai_identifier_str oai:localhost:20.500.12123/14567
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spelling Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variantsRaschia, Maria AgustinaCaffaro, María EugeniaRossi, Ursula AmarantaPoli, Mario AndresGanado BovinoMétodosCaseínaBeta-caseínaLeche de VacaCattleMethodsCaseinBeta-caseinCow MilkPCRA growing interest in the production and commercialization of A2 cow's milk has been observed in many countries in the last few years due to the beneficial properties for human health attributed to A2 β-casein variant. Methods of varying complexity and different equipment requirements have been proposed for the determination of the β-casein genotype of individual cows. We proposed herein a modification of a previously patented method based on an amplification-created restriction site PCR followed by restriction fragment length polymorphism analysis. This method allows to identify and differentiate A2-like from A1-like β-casein variants, after differential endonuclease cleavage flanking the nucleotide that determines the amino acid at position 67 of β-casein. The advantages of this method are that it: • enables to unequivocally score A2-like as well as A1-like β-casein variants, • can be performed at low cost in simply equipped molecular biology laboratories, and • can be scaled up to analyze hundreds of samples per day. For these reasons, and based on the results obtained from the analysis carried out in this work, it showed to be a reliable method for the screening of herds to selective breeding of homozygous cows and bulls for A2 or A2-like alleles.Instituto de GenéticaFil: Raschia, Maria Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Rossi, Ursula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Rossi, Ursula. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaElsevier2023-04-24T12:19:25Z2023-04-24T12:19:25Z2023-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14567https://www.sciencedirect.com/science/article/pii/S22150161230018382215-0161https://doi.org/10.1016/j.mex.2023.102183MethodsX 10 : 102183. (April 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E1-I018-001/2019-PE-E1-I018-001/AR./Crecimiento del rodeo lecheroinfo:eu-repograntAgreement/INTA/2019-PE-E6-I114-001/2019-PE-E6-I114-001/AR./Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.info:eu-repograntAgreement/INTA/2019-PE-E6-I145-001/2019-PE-E6-I145-001/AR./Mejora genética objetiva para aumentar la eficiencia de los sistemas de producción animal.info:eu-repograntAgreement/INTA/2019-PT-E6-I513-001/2019-PT-E6-I513-001/AR./Plataforma de mejoramiento animalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:58Zoai:localhost:20.500.12123/14567instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:58.882INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants
title Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants
spellingShingle Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants
Raschia, Maria Agustina
Ganado Bovino
Métodos
Caseína
Beta-caseína
Leche de Vaca
Cattle
Methods
Casein
Beta-casein
Cow Milk
PCR
title_short Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants
title_full Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants
title_fullStr Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants
title_full_unstemmed Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants
title_sort Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants
dc.creator.none.fl_str_mv Raschia, Maria Agustina
Caffaro, María Eugenia
Rossi, Ursula Amaranta
Poli, Mario Andres
author Raschia, Maria Agustina
author_facet Raschia, Maria Agustina
Caffaro, María Eugenia
Rossi, Ursula Amaranta
Poli, Mario Andres
author_role author
author2 Caffaro, María Eugenia
Rossi, Ursula Amaranta
Poli, Mario Andres
author2_role author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Métodos
Caseína
Beta-caseína
Leche de Vaca
Cattle
Methods
Casein
Beta-casein
Cow Milk
PCR
topic Ganado Bovino
Métodos
Caseína
Beta-caseína
Leche de Vaca
Cattle
Methods
Casein
Beta-casein
Cow Milk
PCR
dc.description.none.fl_txt_mv A growing interest in the production and commercialization of A2 cow's milk has been observed in many countries in the last few years due to the beneficial properties for human health attributed to A2 β-casein variant. Methods of varying complexity and different equipment requirements have been proposed for the determination of the β-casein genotype of individual cows. We proposed herein a modification of a previously patented method based on an amplification-created restriction site PCR followed by restriction fragment length polymorphism analysis. This method allows to identify and differentiate A2-like from A1-like β-casein variants, after differential endonuclease cleavage flanking the nucleotide that determines the amino acid at position 67 of β-casein. The advantages of this method are that it: • enables to unequivocally score A2-like as well as A1-like β-casein variants, • can be performed at low cost in simply equipped molecular biology laboratories, and • can be scaled up to analyze hundreds of samples per day. For these reasons, and based on the results obtained from the analysis carried out in this work, it showed to be a reliable method for the screening of herds to selective breeding of homozygous cows and bulls for A2 or A2-like alleles.
Instituto de Genética
Fil: Raschia, Maria Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Rossi, Ursula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Rossi, Ursula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
description A growing interest in the production and commercialization of A2 cow's milk has been observed in many countries in the last few years due to the beneficial properties for human health attributed to A2 β-casein variant. Methods of varying complexity and different equipment requirements have been proposed for the determination of the β-casein genotype of individual cows. We proposed herein a modification of a previously patented method based on an amplification-created restriction site PCR followed by restriction fragment length polymorphism analysis. This method allows to identify and differentiate A2-like from A1-like β-casein variants, after differential endonuclease cleavage flanking the nucleotide that determines the amino acid at position 67 of β-casein. The advantages of this method are that it: • enables to unequivocally score A2-like as well as A1-like β-casein variants, • can be performed at low cost in simply equipped molecular biology laboratories, and • can be scaled up to analyze hundreds of samples per day. For these reasons, and based on the results obtained from the analysis carried out in this work, it showed to be a reliable method for the screening of herds to selective breeding of homozygous cows and bulls for A2 or A2-like alleles.
publishDate 2023
dc.date.none.fl_str_mv 2023-04-24T12:19:25Z
2023-04-24T12:19:25Z
2023-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14567
https://www.sciencedirect.com/science/article/pii/S2215016123001838
2215-0161
https://doi.org/10.1016/j.mex.2023.102183
url http://hdl.handle.net/20.500.12123/14567
https://www.sciencedirect.com/science/article/pii/S2215016123001838
https://doi.org/10.1016/j.mex.2023.102183
identifier_str_mv 2215-0161
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E1-I018-001/2019-PE-E1-I018-001/AR./Crecimiento del rodeo lechero
info:eu-repograntAgreement/INTA/2019-PE-E6-I114-001/2019-PE-E6-I114-001/AR./Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.
info:eu-repograntAgreement/INTA/2019-PE-E6-I145-001/2019-PE-E6-I145-001/AR./Mejora genética objetiva para aumentar la eficiencia de los sistemas de producción animal.
info:eu-repograntAgreement/INTA/2019-PT-E6-I513-001/2019-PT-E6-I513-001/AR./Plataforma de mejoramiento animal
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv MethodsX 10 : 102183. (April 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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