Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy

Autores
Sánchez-Martín, María Jesús; Gaggiotti, Monica Del Carmen; Simonelli, Laura; Marini, Carlo; Marini, Federico; Boada, Roberto; Llugany, Mercè; Valiente, Manuel; Céccoli, Gabriel; Stoffel, María Micaela; Viltres-Portales, Marcia; Picco, Juan Manuel; Guevara, María Gabriela; Muñoz, Fernando Felipe
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Selenium (Se) is an essential micronutrient, yet its deficiency remains a global concern. This study investigates the biofortification of alfalfa (Medicago sativa cv. ProINTA Super Monarca GR9) via foliar Se application to enhance Se accumulation and transformation into bioavailable organic forms. A controlled environment experiment in a plant growth chamber and a one-season open-field trial (January 2023, Argentina) were conducted. Treatments included sodium selenate (Se(VI)), sodium selenite (Se(IV)), and a 1:1 mixture, applied at 45 and 90 g Se ha−1, with and without the biostimulant BIOFORGE®. Treated plants exhibited increased Se content, correlating with the applied doses. X-ray absorption spectroscopy (XAS) confirmed that most inorganic Se was transformed into organic Se forms, with Se(IV) treatments yielding the highest concentrations of organic Se species such as selenocysteine (SeCys) and selenomethionine (SeMet). Open-field trials showed a complete conversion of Se, though total Se accumulation was lower than in controlled conditions. Se treatments did not affect forage quality or biomass production. The biostimulant slightly reduced Se uptake but did not compromise biofortification. These results highlight Se(IV) as the optimal treatment for alfalfa biofortification, presenting a sustainable strategy to enhance dietary Se intake through functional foods.
EEA Rafaela
Fil: Sánchez-Martín, María Jesús. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; España
Fil: Gaggiotti, Monica Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Gaggiotti, Monica Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Simonelli, Laura. ALBA Synchrotron; España
Fil: Marini, Carlo. ALBA Synchrotron; España
Fil: Marini, Federico. Sapienza University of Rome. Department of Chemistry; Italia
Fil: Boada, Roberto. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; España
Fil: Llugany, Mercè. Universitat Autonòma de Barcelona. Faculty of Biosciences. Plant Physiology Group (BABVE); España
Fil: Valiente, Manuel. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; España
Fil: Céccoli, Gabriel. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Céccoli, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro); Argentina
Fil: Céccoli, Gabriel. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro); Argentina
Fil: Stoffel, María Micaela. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Viltres-Portales, Marcia. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; España
Fil: Picco, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Guevara, María Gabriela. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas (IIB); Argentina
Fil: Guevara, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas (IIB); Argentina
Fil: Muñoz, Fernando Felipe. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Muñoz, Fernando Felipe. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas (IIB); Argentina
Fil: Muñoz, Fernando Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas (IIB); Argentina
Fuente
Agronomy 15 (3) : 580. (February 2025)
Materia
Selenio
Experimentación en Laboratorio
Experimentación en Campo
Espectros Copia de Rayos X
Espectroscopia
Selenium
Medicago sativa
Laboratory Experimentation
Field Experimentation
X-ray Spectroscopy
Spectroscopy
Alfalfa
Lucerne
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21517
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21517
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network_name_str INTA Digital (INTA)
spelling Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray SpectroscopySánchez-Martín, María JesúsGaggiotti, Monica Del CarmenSimonelli, LauraMarini, CarloMarini, FedericoBoada, RobertoLlugany, MercèValiente, ManuelCéccoli, GabrielStoffel, María MicaelaViltres-Portales, MarciaPicco, Juan ManuelGuevara, María GabrielaMuñoz, Fernando FelipeSelenioExperimentación en LaboratorioExperimentación en CampoEspectros Copia de Rayos XEspectroscopiaSeleniumMedicago sativaLaboratory ExperimentationField ExperimentationX-ray SpectroscopySpectroscopyAlfalfaLucerneSelenium (Se) is an essential micronutrient, yet its deficiency remains a global concern. This study investigates the biofortification of alfalfa (Medicago sativa cv. ProINTA Super Monarca GR9) via foliar Se application to enhance Se accumulation and transformation into bioavailable organic forms. A controlled environment experiment in a plant growth chamber and a one-season open-field trial (January 2023, Argentina) were conducted. Treatments included sodium selenate (Se(VI)), sodium selenite (Se(IV)), and a 1:1 mixture, applied at 45 and 90 g Se ha−1, with and without the biostimulant BIOFORGE®. Treated plants exhibited increased Se content, correlating with the applied doses. X-ray absorption spectroscopy (XAS) confirmed that most inorganic Se was transformed into organic Se forms, with Se(IV) treatments yielding the highest concentrations of organic Se species such as selenocysteine (SeCys) and selenomethionine (SeMet). Open-field trials showed a complete conversion of Se, though total Se accumulation was lower than in controlled conditions. Se treatments did not affect forage quality or biomass production. The biostimulant slightly reduced Se uptake but did not compromise biofortification. These results highlight Se(IV) as the optimal treatment for alfalfa biofortification, presenting a sustainable strategy to enhance dietary Se intake through functional foods.EEA RafaelaFil: Sánchez-Martín, María Jesús. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; EspañaFil: Gaggiotti, Monica Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Gaggiotti, Monica Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Simonelli, Laura. ALBA Synchrotron; EspañaFil: Marini, Carlo. ALBA Synchrotron; EspañaFil: Marini, Federico. Sapienza University of Rome. Department of Chemistry; ItaliaFil: Boada, Roberto. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; EspañaFil: Llugany, Mercè. Universitat Autonòma de Barcelona. Faculty of Biosciences. Plant Physiology Group (BABVE); EspañaFil: Valiente, Manuel. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; EspañaFil: Céccoli, Gabriel. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; ArgentinaFil: Céccoli, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro); ArgentinaFil: Céccoli, Gabriel. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro); ArgentinaFil: Stoffel, María Micaela. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; ArgentinaFil: Viltres-Portales, Marcia. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; EspañaFil: Picco, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Guevara, María Gabriela. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas (IIB); ArgentinaFil: Guevara, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas (IIB); ArgentinaFil: Muñoz, Fernando Felipe. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; ArgentinaFil: Muñoz, Fernando Felipe. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas (IIB); ArgentinaFil: Muñoz, Fernando Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas (IIB); ArgentinaMDPI2025-02-28T13:48:58Z2025-02-28T13:48:58Z2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21517https://www.mdpi.com/2073-4395/15/3/5802073-4395https://doi.org/10.3390/agronomy15030580Agronomy 15 (3) : 580. (February 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:19:25Zoai:localhost:20.500.12123/21517instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:19:25.565INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy
title Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy
spellingShingle Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy
Sánchez-Martín, María Jesús
Selenio
Experimentación en Laboratorio
Experimentación en Campo
Espectros Copia de Rayos X
Espectroscopia
Selenium
Medicago sativa
Laboratory Experimentation
Field Experimentation
X-ray Spectroscopy
Spectroscopy
Alfalfa
Lucerne
title_short Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy
title_full Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy
title_fullStr Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy
title_full_unstemmed Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy
title_sort Enhancing Organic Selenium Forms in Alfalfa Forage Through Inorganic Selenium Foliar Application: Insights from Laboratory and Field Studies Using X-Ray Spectroscopy
dc.creator.none.fl_str_mv Sánchez-Martín, María Jesús
Gaggiotti, Monica Del Carmen
Simonelli, Laura
Marini, Carlo
Marini, Federico
Boada, Roberto
Llugany, Mercè
Valiente, Manuel
Céccoli, Gabriel
Stoffel, María Micaela
Viltres-Portales, Marcia
Picco, Juan Manuel
Guevara, María Gabriela
Muñoz, Fernando Felipe
author Sánchez-Martín, María Jesús
author_facet Sánchez-Martín, María Jesús
Gaggiotti, Monica Del Carmen
Simonelli, Laura
Marini, Carlo
Marini, Federico
Boada, Roberto
Llugany, Mercè
Valiente, Manuel
Céccoli, Gabriel
Stoffel, María Micaela
Viltres-Portales, Marcia
Picco, Juan Manuel
Guevara, María Gabriela
Muñoz, Fernando Felipe
author_role author
author2 Gaggiotti, Monica Del Carmen
Simonelli, Laura
Marini, Carlo
Marini, Federico
Boada, Roberto
Llugany, Mercè
Valiente, Manuel
Céccoli, Gabriel
Stoffel, María Micaela
Viltres-Portales, Marcia
Picco, Juan Manuel
Guevara, María Gabriela
Muñoz, Fernando Felipe
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Selenio
Experimentación en Laboratorio
Experimentación en Campo
Espectros Copia de Rayos X
Espectroscopia
Selenium
Medicago sativa
Laboratory Experimentation
Field Experimentation
X-ray Spectroscopy
Spectroscopy
Alfalfa
Lucerne
topic Selenio
Experimentación en Laboratorio
Experimentación en Campo
Espectros Copia de Rayos X
Espectroscopia
Selenium
Medicago sativa
Laboratory Experimentation
Field Experimentation
X-ray Spectroscopy
Spectroscopy
Alfalfa
Lucerne
dc.description.none.fl_txt_mv Selenium (Se) is an essential micronutrient, yet its deficiency remains a global concern. This study investigates the biofortification of alfalfa (Medicago sativa cv. ProINTA Super Monarca GR9) via foliar Se application to enhance Se accumulation and transformation into bioavailable organic forms. A controlled environment experiment in a plant growth chamber and a one-season open-field trial (January 2023, Argentina) were conducted. Treatments included sodium selenate (Se(VI)), sodium selenite (Se(IV)), and a 1:1 mixture, applied at 45 and 90 g Se ha−1, with and without the biostimulant BIOFORGE®. Treated plants exhibited increased Se content, correlating with the applied doses. X-ray absorption spectroscopy (XAS) confirmed that most inorganic Se was transformed into organic Se forms, with Se(IV) treatments yielding the highest concentrations of organic Se species such as selenocysteine (SeCys) and selenomethionine (SeMet). Open-field trials showed a complete conversion of Se, though total Se accumulation was lower than in controlled conditions. Se treatments did not affect forage quality or biomass production. The biostimulant slightly reduced Se uptake but did not compromise biofortification. These results highlight Se(IV) as the optimal treatment for alfalfa biofortification, presenting a sustainable strategy to enhance dietary Se intake through functional foods.
EEA Rafaela
Fil: Sánchez-Martín, María Jesús. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; España
Fil: Gaggiotti, Monica Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Gaggiotti, Monica Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Simonelli, Laura. ALBA Synchrotron; España
Fil: Marini, Carlo. ALBA Synchrotron; España
Fil: Marini, Federico. Sapienza University of Rome. Department of Chemistry; Italia
Fil: Boada, Roberto. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; España
Fil: Llugany, Mercè. Universitat Autonòma de Barcelona. Faculty of Biosciences. Plant Physiology Group (BABVE); España
Fil: Valiente, Manuel. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; España
Fil: Céccoli, Gabriel. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Céccoli, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro); Argentina
Fil: Céccoli, Gabriel. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Instituto de Ciencias Agropecuarias del Litoral (ICiAgro); Argentina
Fil: Stoffel, María Micaela. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Viltres-Portales, Marcia. Universitat Autònoma de Barcelona. Faculty of Science. Department of Chemistry. GTS Research Group; España
Fil: Picco, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Guevara, María Gabriela. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas (IIB); Argentina
Fil: Guevara, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas (IIB); Argentina
Fil: Muñoz, Fernando Felipe. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Muñoz, Fernando Felipe. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Biológicas (IIB); Argentina
Fil: Muñoz, Fernando Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas (IIB); Argentina
description Selenium (Se) is an essential micronutrient, yet its deficiency remains a global concern. This study investigates the biofortification of alfalfa (Medicago sativa cv. ProINTA Super Monarca GR9) via foliar Se application to enhance Se accumulation and transformation into bioavailable organic forms. A controlled environment experiment in a plant growth chamber and a one-season open-field trial (January 2023, Argentina) were conducted. Treatments included sodium selenate (Se(VI)), sodium selenite (Se(IV)), and a 1:1 mixture, applied at 45 and 90 g Se ha−1, with and without the biostimulant BIOFORGE®. Treated plants exhibited increased Se content, correlating with the applied doses. X-ray absorption spectroscopy (XAS) confirmed that most inorganic Se was transformed into organic Se forms, with Se(IV) treatments yielding the highest concentrations of organic Se species such as selenocysteine (SeCys) and selenomethionine (SeMet). Open-field trials showed a complete conversion of Se, though total Se accumulation was lower than in controlled conditions. Se treatments did not affect forage quality or biomass production. The biostimulant slightly reduced Se uptake but did not compromise biofortification. These results highlight Se(IV) as the optimal treatment for alfalfa biofortification, presenting a sustainable strategy to enhance dietary Se intake through functional foods.
publishDate 2025
dc.date.none.fl_str_mv 2025-02-28T13:48:58Z
2025-02-28T13:48:58Z
2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21517
https://www.mdpi.com/2073-4395/15/3/580
2073-4395
https://doi.org/10.3390/agronomy15030580
url http://hdl.handle.net/20.500.12123/21517
https://www.mdpi.com/2073-4395/15/3/580
https://doi.org/10.3390/agronomy15030580
identifier_str_mv 2073-4395
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Agronomy 15 (3) : 580. (February 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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