Tomato Abscisic Acid Stress ripening (ASR) gene family revisited

Autores
Golan, Ido; Dominguez, Pia Guadalupe; Konrad, Zvia; Shkolnik Inbar, Doron; Carrari, Fernando; Bar Zvi, Dudy
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tomato ABSCISIC ACID RIPENING 1 (ASR1) was the first cloned plant ASR gene. ASR orthologs were then cloned from a large number of monocot, dicot and gymnosperm plants, where they are mostly involved in response to abiotic (drought and salinity) stress and fruit ripening. The tomato genome encodes five ASR genes: ASR1, 2, 3 and 5 encode low-molecular-weight proteins (ca. 110 amino acid residues each), whereas ASR4 encodes a 297-residue polypeptide. Information on the expression of the tomato ASR gene family is scarce. We used quantitative RT-PCR to assay the expression of this gene family in plant development and in response to salt and osmotic stresses. ASR1 and ASR4 were the main expressed genes in all tested organs and conditions, whereas ASR2 and ASR3/5 expression was two to three orders of magnitude lower (with the exception of cotyledons). ASR1 is expressed in all plant tissues tested whereas ASR4 expression is limited to photosynthetic organs and stamens. Essentially, ASR1 accounted for most of ASR gene expression in roots, stems and fruits at all developmental stages, whereas ASR4 was the major gene expressed in cotyledons and young and fully developed leaves. Both ASR1 and ASR4 were expressed in flower organs, with ASR1 expression dominating in stamens and pistils, ASR4 in sepals and petals. Steady-state levels of ASR1 and ASR4 were upregulated in plant vegetative organs following exposure to salt stress, osmotic stress or the plant abiotic stress hormone abscisic acid (ABA). Tomato plants overexpressing ASR1 displayed enhanced survival rates under conditions of water stress, whereas ASR1-antisense plants displayed marginal hypersensitivity to water withholding.
Instituto de Biotecnología
Fil: Golan, Ido. Ben-Gurion University of the Negev. Doris and Bertie Black Center for Bioenergetics in Life Sciences. Department of Life Sciences; Israel
Fil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Konrad, Zvia. Ben-Gurion University of the Negev. Doris and Bertie Black Center for Bioenergetics in Life Sciences. Department of Life Sciences; Israel
Fil: Shkolnik Inbar, Doron. Ben-Gurion University of the Negev. Doris and Bertie Black Center for Bioenergetics in Life Sciences. Department of Life Sciences; Israel
Fuente
PLoS ONE 9 (10) : e107117. (October 13, 2014)
Materia
Tomate
Solanum Lycopersicum
Hojas
Fruto
Expresión Génica
Maduración
Estrés Abiótico
ABA
Estrés Osmótico
Plantas Transgénicas
Resistencia Fisiológica al Estrés
Tomatoes
Leaves
Fruit
Gene Expression
Maturation
Abiotic Stress
Osmotic Stress
Transgenic Plants
Physiological Stress Resistance
Ácido Abscísico
Abscisic Acid
Asr1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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Acceder
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oai_identifier_str oai:localhost:20.500.12123/4759
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Tomato Abscisic Acid Stress ripening (ASR) gene family revisitedGolan, IdoDominguez, Pia GuadalupeKonrad, ZviaShkolnik Inbar, DoronCarrari, FernandoBar Zvi, DudyTomateSolanum LycopersicumHojasFrutoExpresión GénicaMaduraciónEstrés AbióticoABAEstrés OsmóticoPlantas TransgénicasResistencia Fisiológica al EstrésTomatoesLeavesFruitGene ExpressionMaturationAbiotic StressOsmotic StressTransgenic PlantsPhysiological Stress ResistanceÁcido AbscísicoAbscisic AcidAsr1Tomato ABSCISIC ACID RIPENING 1 (ASR1) was the first cloned plant ASR gene. ASR orthologs were then cloned from a large number of monocot, dicot and gymnosperm plants, where they are mostly involved in response to abiotic (drought and salinity) stress and fruit ripening. The tomato genome encodes five ASR genes: ASR1, 2, 3 and 5 encode low-molecular-weight proteins (ca. 110 amino acid residues each), whereas ASR4 encodes a 297-residue polypeptide. Information on the expression of the tomato ASR gene family is scarce. We used quantitative RT-PCR to assay the expression of this gene family in plant development and in response to salt and osmotic stresses. ASR1 and ASR4 were the main expressed genes in all tested organs and conditions, whereas ASR2 and ASR3/5 expression was two to three orders of magnitude lower (with the exception of cotyledons). ASR1 is expressed in all plant tissues tested whereas ASR4 expression is limited to photosynthetic organs and stamens. Essentially, ASR1 accounted for most of ASR gene expression in roots, stems and fruits at all developmental stages, whereas ASR4 was the major gene expressed in cotyledons and young and fully developed leaves. Both ASR1 and ASR4 were expressed in flower organs, with ASR1 expression dominating in stamens and pistils, ASR4 in sepals and petals. Steady-state levels of ASR1 and ASR4 were upregulated in plant vegetative organs following exposure to salt stress, osmotic stress or the plant abiotic stress hormone abscisic acid (ABA). Tomato plants overexpressing ASR1 displayed enhanced survival rates under conditions of water stress, whereas ASR1-antisense plants displayed marginal hypersensitivity to water withholding.Instituto de BiotecnologíaFil: Golan, Ido. Ben-Gurion University of the Negev. Doris and Bertie Black Center for Bioenergetics in Life Sciences. Department of Life Sciences; IsraelFil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Konrad, Zvia. Ben-Gurion University of the Negev. Doris and Bertie Black Center for Bioenergetics in Life Sciences. Department of Life Sciences; IsraelFil: Shkolnik Inbar, Doron. Ben-Gurion University of the Negev. Doris and Bertie Black Center for Bioenergetics in Life Sciences. Department of Life Sciences; IsraelPLOS2019-03-27T14:45:25Z2019-03-27T14:45:25Z2014-10-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4759https://journals.plos.org/plosone/article?id=10.1371/journal.pone.01071171932-6203https://doi.org/10.1371/journal.pone.0107117PLoS ONE 9 (10) : e107117. (October 13, 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:54Zoai:localhost:20.500.12123/4759instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:54.431INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Tomato Abscisic Acid Stress ripening (ASR) gene family revisited
title Tomato Abscisic Acid Stress ripening (ASR) gene family revisited
spellingShingle Tomato Abscisic Acid Stress ripening (ASR) gene family revisited
Golan, Ido
Tomate
Solanum Lycopersicum
Hojas
Fruto
Expresión Génica
Maduración
Estrés Abiótico
ABA
Estrés Osmótico
Plantas Transgénicas
Resistencia Fisiológica al Estrés
Tomatoes
Leaves
Fruit
Gene Expression
Maturation
Abiotic Stress
Osmotic Stress
Transgenic Plants
Physiological Stress Resistance
Ácido Abscísico
Abscisic Acid
Asr1
title_short Tomato Abscisic Acid Stress ripening (ASR) gene family revisited
title_full Tomato Abscisic Acid Stress ripening (ASR) gene family revisited
title_fullStr Tomato Abscisic Acid Stress ripening (ASR) gene family revisited
title_full_unstemmed Tomato Abscisic Acid Stress ripening (ASR) gene family revisited
title_sort Tomato Abscisic Acid Stress ripening (ASR) gene family revisited
dc.creator.none.fl_str_mv Golan, Ido
Dominguez, Pia Guadalupe
Konrad, Zvia
Shkolnik Inbar, Doron
Carrari, Fernando
Bar Zvi, Dudy
author Golan, Ido
author_facet Golan, Ido
Dominguez, Pia Guadalupe
Konrad, Zvia
Shkolnik Inbar, Doron
Carrari, Fernando
Bar Zvi, Dudy
author_role author
author2 Dominguez, Pia Guadalupe
Konrad, Zvia
Shkolnik Inbar, Doron
Carrari, Fernando
Bar Zvi, Dudy
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Tomate
Solanum Lycopersicum
Hojas
Fruto
Expresión Génica
Maduración
Estrés Abiótico
ABA
Estrés Osmótico
Plantas Transgénicas
Resistencia Fisiológica al Estrés
Tomatoes
Leaves
Fruit
Gene Expression
Maturation
Abiotic Stress
Osmotic Stress
Transgenic Plants
Physiological Stress Resistance
Ácido Abscísico
Abscisic Acid
Asr1
topic Tomate
Solanum Lycopersicum
Hojas
Fruto
Expresión Génica
Maduración
Estrés Abiótico
ABA
Estrés Osmótico
Plantas Transgénicas
Resistencia Fisiológica al Estrés
Tomatoes
Leaves
Fruit
Gene Expression
Maturation
Abiotic Stress
Osmotic Stress
Transgenic Plants
Physiological Stress Resistance
Ácido Abscísico
Abscisic Acid
Asr1
dc.description.none.fl_txt_mv Tomato ABSCISIC ACID RIPENING 1 (ASR1) was the first cloned plant ASR gene. ASR orthologs were then cloned from a large number of monocot, dicot and gymnosperm plants, where they are mostly involved in response to abiotic (drought and salinity) stress and fruit ripening. The tomato genome encodes five ASR genes: ASR1, 2, 3 and 5 encode low-molecular-weight proteins (ca. 110 amino acid residues each), whereas ASR4 encodes a 297-residue polypeptide. Information on the expression of the tomato ASR gene family is scarce. We used quantitative RT-PCR to assay the expression of this gene family in plant development and in response to salt and osmotic stresses. ASR1 and ASR4 were the main expressed genes in all tested organs and conditions, whereas ASR2 and ASR3/5 expression was two to three orders of magnitude lower (with the exception of cotyledons). ASR1 is expressed in all plant tissues tested whereas ASR4 expression is limited to photosynthetic organs and stamens. Essentially, ASR1 accounted for most of ASR gene expression in roots, stems and fruits at all developmental stages, whereas ASR4 was the major gene expressed in cotyledons and young and fully developed leaves. Both ASR1 and ASR4 were expressed in flower organs, with ASR1 expression dominating in stamens and pistils, ASR4 in sepals and petals. Steady-state levels of ASR1 and ASR4 were upregulated in plant vegetative organs following exposure to salt stress, osmotic stress or the plant abiotic stress hormone abscisic acid (ABA). Tomato plants overexpressing ASR1 displayed enhanced survival rates under conditions of water stress, whereas ASR1-antisense plants displayed marginal hypersensitivity to water withholding.
Instituto de Biotecnología
Fil: Golan, Ido. Ben-Gurion University of the Negev. Doris and Bertie Black Center for Bioenergetics in Life Sciences. Department of Life Sciences; Israel
Fil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Konrad, Zvia. Ben-Gurion University of the Negev. Doris and Bertie Black Center for Bioenergetics in Life Sciences. Department of Life Sciences; Israel
Fil: Shkolnik Inbar, Doron. Ben-Gurion University of the Negev. Doris and Bertie Black Center for Bioenergetics in Life Sciences. Department of Life Sciences; Israel
description Tomato ABSCISIC ACID RIPENING 1 (ASR1) was the first cloned plant ASR gene. ASR orthologs were then cloned from a large number of monocot, dicot and gymnosperm plants, where they are mostly involved in response to abiotic (drought and salinity) stress and fruit ripening. The tomato genome encodes five ASR genes: ASR1, 2, 3 and 5 encode low-molecular-weight proteins (ca. 110 amino acid residues each), whereas ASR4 encodes a 297-residue polypeptide. Information on the expression of the tomato ASR gene family is scarce. We used quantitative RT-PCR to assay the expression of this gene family in plant development and in response to salt and osmotic stresses. ASR1 and ASR4 were the main expressed genes in all tested organs and conditions, whereas ASR2 and ASR3/5 expression was two to three orders of magnitude lower (with the exception of cotyledons). ASR1 is expressed in all plant tissues tested whereas ASR4 expression is limited to photosynthetic organs and stamens. Essentially, ASR1 accounted for most of ASR gene expression in roots, stems and fruits at all developmental stages, whereas ASR4 was the major gene expressed in cotyledons and young and fully developed leaves. Both ASR1 and ASR4 were expressed in flower organs, with ASR1 expression dominating in stamens and pistils, ASR4 in sepals and petals. Steady-state levels of ASR1 and ASR4 were upregulated in plant vegetative organs following exposure to salt stress, osmotic stress or the plant abiotic stress hormone abscisic acid (ABA). Tomato plants overexpressing ASR1 displayed enhanced survival rates under conditions of water stress, whereas ASR1-antisense plants displayed marginal hypersensitivity to water withholding.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-13
2019-03-27T14:45:25Z
2019-03-27T14:45:25Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4759
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0107117
1932-6203
https://doi.org/10.1371/journal.pone.0107117
url http://hdl.handle.net/20.500.12123/4759
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0107117
https://doi.org/10.1371/journal.pone.0107117
identifier_str_mv 1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
dc.source.none.fl_str_mv PLoS ONE 9 (10) : e107117. (October 13, 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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