Vitrified embryo transfer in Merino sheep under extensive conditions

Autores
Gibbons, Alejandro Eduardo; Bruno Galarraga, Maria Macarena; Fernandez, Jimena; Gonzalez Bulnes, Antonio; Cueto, Marcela Isabel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim was to evaluate pregnancy success after transfer of embryos vitrified in micropipette tips in Merino sheep under extensive conditions. A second objective was to evaluate the influence of embryo stage in such pregnancy rate. One hundred and twenty-seven embryos were rewarmed and transferred into recipient ewes. On rewarming, the embryos were placed into three-step cryoprotectant dilutions. Finally, prior to transfer to recipient females, embryos were maintained in Basic Medium for 5 min at 25ºC and were re-evaluated by morphological criteria; all degenerated embryos were eliminated. Recipient ewes (n = 150) were treated for estrus with sponges placed for 14 days and 300 IU of eCG. At embryo transfer, three experimental groups were defined: morulae transferred on Day 7, blastocysts transferred on Day 7 and blastocysts transferred on Day 8 after sponge removal. In all groups, semi-laparoscopic transfer of one rewarmed embryo per recipient was performed. Pregnancy was diagnosed by ultrasonography on day 28 after embryo transfer. The embryo selection rate after rewarming was higher for blastocysts (89.3% - 67/75) compared to morulae (65.9% - 60/91) (P < 0.05). Pregnancy diagnosis showed a 38.3% (23/60) of success after morula transfer on Day 7 post progestagen removal. The day of transfer showed a significant influence on pregnancy rate after blastocyst transfer (Day 8, 55.9% - 19/34 vs Day 7, 21.2% - 7/33) (P < 0.05). Blastocysts transfer on Day 8 showed the highest global efficiency (pregnancies/total embryos after rewarming) (47.5% - 19/40) (P < 0.05). In conclusion, reproductive efficiency obtained by vitrified embryo transfer allows its recommendation for embryo transfer programs under extensive conditions. The importance of considering the synchrony between the embryo age and the recipient uterus stage is emphasized.
Estación Experimental Agropecuaria Bariloche
Fil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Laboratorio de Reproducción de Rumiantes Menores; Argentina
Fil: Bruno Galarraga, María Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Laboratorio de Reproducción de Rumiantes Menores; Argentina
Fil: Fernández, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Laboratorio de Reproducción de Rumiantes Menores; Argentina
Fil: Gonzalez Bulnes, Antonio. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Departamento de Reproducción Animal; España
Fil: Cueto, Marcela Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Laboratorio de Reproducción de Rumiantes Menores; Argentina
Fuente
Animal Reproduction 16 (2) : 297-301 (Junio 2019)
Materia
Inseminación Artificial
Inseminación in Vitro
Reproducción Dirigida
Transferencia de Embriones
Artificial Insemination
In Vitro Insemination
Reproduction Control
Embryo Transfer
Sheep
Ovinos
Raza Merino
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5229
Acceder
id INTADig_090c7e78897de0286963eb0434268811
oai_identifier_str oai:localhost:20.500.12123/5229
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Vitrified embryo transfer in Merino sheep under extensive conditionsGibbons, Alejandro EduardoBruno Galarraga, Maria MacarenaFernandez, JimenaGonzalez Bulnes, AntonioCueto, Marcela IsabelInseminación ArtificialInseminación in VitroReproducción DirigidaTransferencia de EmbrionesArtificial InseminationIn Vitro InseminationReproduction ControlEmbryo TransferSheepOvinosRaza MerinoThe aim was to evaluate pregnancy success after transfer of embryos vitrified in micropipette tips in Merino sheep under extensive conditions. A second objective was to evaluate the influence of embryo stage in such pregnancy rate. One hundred and twenty-seven embryos were rewarmed and transferred into recipient ewes. On rewarming, the embryos were placed into three-step cryoprotectant dilutions. Finally, prior to transfer to recipient females, embryos were maintained in Basic Medium for 5 min at 25ºC and were re-evaluated by morphological criteria; all degenerated embryos were eliminated. Recipient ewes (n = 150) were treated for estrus with sponges placed for 14 days and 300 IU of eCG. At embryo transfer, three experimental groups were defined: morulae transferred on Day 7, blastocysts transferred on Day 7 and blastocysts transferred on Day 8 after sponge removal. In all groups, semi-laparoscopic transfer of one rewarmed embryo per recipient was performed. Pregnancy was diagnosed by ultrasonography on day 28 after embryo transfer. The embryo selection rate after rewarming was higher for blastocysts (89.3% - 67/75) compared to morulae (65.9% - 60/91) (P < 0.05). Pregnancy diagnosis showed a 38.3% (23/60) of success after morula transfer on Day 7 post progestagen removal. The day of transfer showed a significant influence on pregnancy rate after blastocyst transfer (Day 8, 55.9% - 19/34 vs Day 7, 21.2% - 7/33) (P < 0.05). Blastocysts transfer on Day 8 showed the highest global efficiency (pregnancies/total embryos after rewarming) (47.5% - 19/40) (P < 0.05). In conclusion, reproductive efficiency obtained by vitrified embryo transfer allows its recommendation for embryo transfer programs under extensive conditions. The importance of considering the synchrony between the embryo age and the recipient uterus stage is emphasized.Estación Experimental Agropecuaria BarilocheFil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Laboratorio de Reproducción de Rumiantes Menores; ArgentinaFil: Bruno Galarraga, María Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Laboratorio de Reproducción de Rumiantes Menores; ArgentinaFil: Fernández, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Laboratorio de Reproducción de Rumiantes Menores; ArgentinaFil: Gonzalez Bulnes, Antonio. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Departamento de Reproducción Animal; EspañaFil: Cueto, Marcela Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Laboratorio de Reproducción de Rumiantes Menores; ArgentinaBrazilian College of Animal Reproduction2019-06-03T12:34:40Z2019-06-03T12:34:40Z2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/5229http://www.animal-reproduction.org/article/doi/10.21451/1984-3143-AR2018-01081984-3143http://dx.doi.org/10.21451/1984-3143-AR2018-0108Animal Reproduction 16 (2) : 297-301 (Junio 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:58Zoai:localhost:20.500.12123/5229instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:59.529INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Vitrified embryo transfer in Merino sheep under extensive conditions
title Vitrified embryo transfer in Merino sheep under extensive conditions
spellingShingle Vitrified embryo transfer in Merino sheep under extensive conditions
Gibbons, Alejandro Eduardo
Inseminación Artificial
Inseminación in Vitro
Reproducción Dirigida
Transferencia de Embriones
Artificial Insemination
In Vitro Insemination
Reproduction Control
Embryo Transfer
Sheep
Ovinos
Raza Merino
title_short Vitrified embryo transfer in Merino sheep under extensive conditions
title_full Vitrified embryo transfer in Merino sheep under extensive conditions
title_fullStr Vitrified embryo transfer in Merino sheep under extensive conditions
title_full_unstemmed Vitrified embryo transfer in Merino sheep under extensive conditions
title_sort Vitrified embryo transfer in Merino sheep under extensive conditions
dc.creator.none.fl_str_mv Gibbons, Alejandro Eduardo
Bruno Galarraga, Maria Macarena
Fernandez, Jimena
Gonzalez Bulnes, Antonio
Cueto, Marcela Isabel
author Gibbons, Alejandro Eduardo
author_facet Gibbons, Alejandro Eduardo
Bruno Galarraga, Maria Macarena
Fernandez, Jimena
Gonzalez Bulnes, Antonio
Cueto, Marcela Isabel
author_role author
author2 Bruno Galarraga, Maria Macarena
Fernandez, Jimena
Gonzalez Bulnes, Antonio
Cueto, Marcela Isabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Inseminación Artificial
Inseminación in Vitro
Reproducción Dirigida
Transferencia de Embriones
Artificial Insemination
In Vitro Insemination
Reproduction Control
Embryo Transfer
Sheep
Ovinos
Raza Merino
topic Inseminación Artificial
Inseminación in Vitro
Reproducción Dirigida
Transferencia de Embriones
Artificial Insemination
In Vitro Insemination
Reproduction Control
Embryo Transfer
Sheep
Ovinos
Raza Merino
dc.description.none.fl_txt_mv The aim was to evaluate pregnancy success after transfer of embryos vitrified in micropipette tips in Merino sheep under extensive conditions. A second objective was to evaluate the influence of embryo stage in such pregnancy rate. One hundred and twenty-seven embryos were rewarmed and transferred into recipient ewes. On rewarming, the embryos were placed into three-step cryoprotectant dilutions. Finally, prior to transfer to recipient females, embryos were maintained in Basic Medium for 5 min at 25ºC and were re-evaluated by morphological criteria; all degenerated embryos were eliminated. Recipient ewes (n = 150) were treated for estrus with sponges placed for 14 days and 300 IU of eCG. At embryo transfer, three experimental groups were defined: morulae transferred on Day 7, blastocysts transferred on Day 7 and blastocysts transferred on Day 8 after sponge removal. In all groups, semi-laparoscopic transfer of one rewarmed embryo per recipient was performed. Pregnancy was diagnosed by ultrasonography on day 28 after embryo transfer. The embryo selection rate after rewarming was higher for blastocysts (89.3% - 67/75) compared to morulae (65.9% - 60/91) (P < 0.05). Pregnancy diagnosis showed a 38.3% (23/60) of success after morula transfer on Day 7 post progestagen removal. The day of transfer showed a significant influence on pregnancy rate after blastocyst transfer (Day 8, 55.9% - 19/34 vs Day 7, 21.2% - 7/33) (P < 0.05). Blastocysts transfer on Day 8 showed the highest global efficiency (pregnancies/total embryos after rewarming) (47.5% - 19/40) (P < 0.05). In conclusion, reproductive efficiency obtained by vitrified embryo transfer allows its recommendation for embryo transfer programs under extensive conditions. The importance of considering the synchrony between the embryo age and the recipient uterus stage is emphasized.
Estación Experimental Agropecuaria Bariloche
Fil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Laboratorio de Reproducción de Rumiantes Menores; Argentina
Fil: Bruno Galarraga, María Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Laboratorio de Reproducción de Rumiantes Menores; Argentina
Fil: Fernández, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Laboratorio de Reproducción de Rumiantes Menores; Argentina
Fil: Gonzalez Bulnes, Antonio. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Departamento de Reproducción Animal; España
Fil: Cueto, Marcela Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Laboratorio de Reproducción de Rumiantes Menores; Argentina
description The aim was to evaluate pregnancy success after transfer of embryos vitrified in micropipette tips in Merino sheep under extensive conditions. A second objective was to evaluate the influence of embryo stage in such pregnancy rate. One hundred and twenty-seven embryos were rewarmed and transferred into recipient ewes. On rewarming, the embryos were placed into three-step cryoprotectant dilutions. Finally, prior to transfer to recipient females, embryos were maintained in Basic Medium for 5 min at 25ºC and were re-evaluated by morphological criteria; all degenerated embryos were eliminated. Recipient ewes (n = 150) were treated for estrus with sponges placed for 14 days and 300 IU of eCG. At embryo transfer, three experimental groups were defined: morulae transferred on Day 7, blastocysts transferred on Day 7 and blastocysts transferred on Day 8 after sponge removal. In all groups, semi-laparoscopic transfer of one rewarmed embryo per recipient was performed. Pregnancy was diagnosed by ultrasonography on day 28 after embryo transfer. The embryo selection rate after rewarming was higher for blastocysts (89.3% - 67/75) compared to morulae (65.9% - 60/91) (P < 0.05). Pregnancy diagnosis showed a 38.3% (23/60) of success after morula transfer on Day 7 post progestagen removal. The day of transfer showed a significant influence on pregnancy rate after blastocyst transfer (Day 8, 55.9% - 19/34 vs Day 7, 21.2% - 7/33) (P < 0.05). Blastocysts transfer on Day 8 showed the highest global efficiency (pregnancies/total embryos after rewarming) (47.5% - 19/40) (P < 0.05). In conclusion, reproductive efficiency obtained by vitrified embryo transfer allows its recommendation for embryo transfer programs under extensive conditions. The importance of considering the synchrony between the embryo age and the recipient uterus stage is emphasized.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-03T12:34:40Z
2019-06-03T12:34:40Z
2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/5229
http://www.animal-reproduction.org/article/doi/10.21451/1984-3143-AR2018-0108
1984-3143
http://dx.doi.org/10.21451/1984-3143-AR2018-0108
url http://hdl.handle.net/20.500.12123/5229
http://www.animal-reproduction.org/article/doi/10.21451/1984-3143-AR2018-0108
http://dx.doi.org/10.21451/1984-3143-AR2018-0108
identifier_str_mv 1984-3143
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian College of Animal Reproduction
publisher.none.fl_str_mv Brazilian College of Animal Reproduction
dc.source.none.fl_str_mv Animal Reproduction 16 (2) : 297-301 (Junio 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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