The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
- Autores
- Darqui, Flavia Soledad; Hopp, Horacio Esteban; Lopez Bilbao, Marisa Gisela
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce herbicide-resistant agronomic crops as well as to direct many genome editing studies. Therefore, the ALS gene has become a model sequence target to improve our technological skills for more precise CRISPR/Cas nucleotide base substitution in plants, which is essential for modulation/modification of gene function as opposed to the more general gene knock out obtained by indels in conventional genome editing studies. This review summarizes the main knowledge and experiences attained from the use of the ALS gene as a target in CRISPR/Cas studies.
Instituto de Biotecnología
Fil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Lopez Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Modern Concepts & Developments in Agronomy 7 (2) : 700-711 (Agosto 2020)
- Materia
-
Mutagenesis
Amino Acids
Resistance to Herbicides
Mutagénesis
Aminoácidos
Resistencia a los Herbicidas
Genes
Acetolactate Synthase
Acetolactato Sintasa - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/7913
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The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?Darqui, Flavia SoledadHopp, Horacio EstebanLopez Bilbao, Marisa GiselaMutagenesisAmino AcidsResistance to HerbicidesMutagénesisAminoácidosResistencia a los HerbicidasGenesAcetolactate SynthaseAcetolactato SintasaSpecific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce herbicide-resistant agronomic crops as well as to direct many genome editing studies. Therefore, the ALS gene has become a model sequence target to improve our technological skills for more precise CRISPR/Cas nucleotide base substitution in plants, which is essential for modulation/modification of gene function as opposed to the more general gene knock out obtained by indels in conventional genome editing studies. This review summarizes the main knowledge and experiences attained from the use of the ALS gene as a target in CRISPR/Cas studies.Instituto de BiotecnologíaFil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Lopez Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaCrimson Publishers2020-09-16T17:47:50Z2020-09-16T17:47:50Z2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7913https://crimsonpublishers.com/mcda/fulltext/MCDA.000656.php2637-7659http://doi.org/10.31031/MCDA.2020.07.000656Modern Concepts & Developments in Agronomy 7 (2) : 700-711 (Agosto 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:53Zoai:localhost:20.500.12123/7913instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:54.025INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? |
title |
The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? |
spellingShingle |
The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? Darqui, Flavia Soledad Mutagenesis Amino Acids Resistance to Herbicides Mutagénesis Aminoácidos Resistencia a los Herbicidas Genes Acetolactate Synthase Acetolactato Sintasa |
title_short |
The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? |
title_full |
The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? |
title_fullStr |
The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? |
title_full_unstemmed |
The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? |
title_sort |
The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? |
dc.creator.none.fl_str_mv |
Darqui, Flavia Soledad Hopp, Horacio Esteban Lopez Bilbao, Marisa Gisela |
author |
Darqui, Flavia Soledad |
author_facet |
Darqui, Flavia Soledad Hopp, Horacio Esteban Lopez Bilbao, Marisa Gisela |
author_role |
author |
author2 |
Hopp, Horacio Esteban Lopez Bilbao, Marisa Gisela |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Mutagenesis Amino Acids Resistance to Herbicides Mutagénesis Aminoácidos Resistencia a los Herbicidas Genes Acetolactate Synthase Acetolactato Sintasa |
topic |
Mutagenesis Amino Acids Resistance to Herbicides Mutagénesis Aminoácidos Resistencia a los Herbicidas Genes Acetolactate Synthase Acetolactato Sintasa |
dc.description.none.fl_txt_mv |
Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce herbicide-resistant agronomic crops as well as to direct many genome editing studies. Therefore, the ALS gene has become a model sequence target to improve our technological skills for more precise CRISPR/Cas nucleotide base substitution in plants, which is essential for modulation/modification of gene function as opposed to the more general gene knock out obtained by indels in conventional genome editing studies. This review summarizes the main knowledge and experiences attained from the use of the ALS gene as a target in CRISPR/Cas studies. Instituto de Biotecnología Fil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina Fil: Lopez Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce herbicide-resistant agronomic crops as well as to direct many genome editing studies. Therefore, the ALS gene has become a model sequence target to improve our technological skills for more precise CRISPR/Cas nucleotide base substitution in plants, which is essential for modulation/modification of gene function as opposed to the more general gene knock out obtained by indels in conventional genome editing studies. This review summarizes the main knowledge and experiences attained from the use of the ALS gene as a target in CRISPR/Cas studies. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-16T17:47:50Z 2020-09-16T17:47:50Z 2020-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/7913 https://crimsonpublishers.com/mcda/fulltext/MCDA.000656.php 2637-7659 http://doi.org/10.31031/MCDA.2020.07.000656 |
url |
http://hdl.handle.net/20.500.12123/7913 https://crimsonpublishers.com/mcda/fulltext/MCDA.000656.php http://doi.org/10.31031/MCDA.2020.07.000656 |
identifier_str_mv |
2637-7659 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Crimson Publishers |
publisher.none.fl_str_mv |
Crimson Publishers |
dc.source.none.fl_str_mv |
Modern Concepts & Developments in Agronomy 7 (2) : 700-711 (Agosto 2020) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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