The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?

Autores
Darqui, Flavia Soledad; Hopp, Horacio Esteban; Lopez Bilbao, Marisa Gisela
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce herbicide-resistant agronomic crops as well as to direct many genome editing studies. Therefore, the ALS gene has become a model sequence target to improve our technological skills for more precise CRISPR/Cas nucleotide base substitution in plants, which is essential for modulation/modification of gene function as opposed to the more general gene knock out obtained by indels in conventional genome editing studies. This review summarizes the main knowledge and experiences attained from the use of the ALS gene as a target in CRISPR/Cas studies.
Instituto de Biotecnología
Fil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Lopez Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Modern Concepts & Developments in Agronomy 7 (2) : 700-711 (Agosto 2020)
Materia
Mutagenesis
Amino Acids
Resistance to Herbicides
Mutagénesis
Aminoácidos
Resistencia a los Herbicidas
Genes
Acetolactate Synthase
Acetolactato Sintasa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7913

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spelling The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?Darqui, Flavia SoledadHopp, Horacio EstebanLopez Bilbao, Marisa GiselaMutagenesisAmino AcidsResistance to HerbicidesMutagénesisAminoácidosResistencia a los HerbicidasGenesAcetolactate SynthaseAcetolactato SintasaSpecific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce herbicide-resistant agronomic crops as well as to direct many genome editing studies. Therefore, the ALS gene has become a model sequence target to improve our technological skills for more precise CRISPR/Cas nucleotide base substitution in plants, which is essential for modulation/modification of gene function as opposed to the more general gene knock out obtained by indels in conventional genome editing studies. This review summarizes the main knowledge and experiences attained from the use of the ALS gene as a target in CRISPR/Cas studies.Instituto de BiotecnologíaFil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Lopez Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaCrimson Publishers2020-09-16T17:47:50Z2020-09-16T17:47:50Z2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7913https://crimsonpublishers.com/mcda/fulltext/MCDA.000656.php2637-7659http://doi.org/10.31031/MCDA.2020.07.000656Modern Concepts & Developments in Agronomy 7 (2) : 700-711 (Agosto 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:53Zoai:localhost:20.500.12123/7913instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:54.025INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
title The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
spellingShingle The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
Darqui, Flavia Soledad
Mutagenesis
Amino Acids
Resistance to Herbicides
Mutagénesis
Aminoácidos
Resistencia a los Herbicidas
Genes
Acetolactate Synthase
Acetolactato Sintasa
title_short The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
title_full The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
title_fullStr The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
title_full_unstemmed The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
title_sort The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
dc.creator.none.fl_str_mv Darqui, Flavia Soledad
Hopp, Horacio Esteban
Lopez Bilbao, Marisa Gisela
author Darqui, Flavia Soledad
author_facet Darqui, Flavia Soledad
Hopp, Horacio Esteban
Lopez Bilbao, Marisa Gisela
author_role author
author2 Hopp, Horacio Esteban
Lopez Bilbao, Marisa Gisela
author2_role author
author
dc.subject.none.fl_str_mv Mutagenesis
Amino Acids
Resistance to Herbicides
Mutagénesis
Aminoácidos
Resistencia a los Herbicidas
Genes
Acetolactate Synthase
Acetolactato Sintasa
topic Mutagenesis
Amino Acids
Resistance to Herbicides
Mutagénesis
Aminoácidos
Resistencia a los Herbicidas
Genes
Acetolactate Synthase
Acetolactato Sintasa
dc.description.none.fl_txt_mv Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce herbicide-resistant agronomic crops as well as to direct many genome editing studies. Therefore, the ALS gene has become a model sequence target to improve our technological skills for more precise CRISPR/Cas nucleotide base substitution in plants, which is essential for modulation/modification of gene function as opposed to the more general gene knock out obtained by indels in conventional genome editing studies. This review summarizes the main knowledge and experiences attained from the use of the ALS gene as a target in CRISPR/Cas studies.
Instituto de Biotecnología
Fil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Lopez Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce herbicide-resistant agronomic crops as well as to direct many genome editing studies. Therefore, the ALS gene has become a model sequence target to improve our technological skills for more precise CRISPR/Cas nucleotide base substitution in plants, which is essential for modulation/modification of gene function as opposed to the more general gene knock out obtained by indels in conventional genome editing studies. This review summarizes the main knowledge and experiences attained from the use of the ALS gene as a target in CRISPR/Cas studies.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-16T17:47:50Z
2020-09-16T17:47:50Z
2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7913
https://crimsonpublishers.com/mcda/fulltext/MCDA.000656.php
2637-7659
http://doi.org/10.31031/MCDA.2020.07.000656
url http://hdl.handle.net/20.500.12123/7913
https://crimsonpublishers.com/mcda/fulltext/MCDA.000656.php
http://doi.org/10.31031/MCDA.2020.07.000656
identifier_str_mv 2637-7659
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Crimson Publishers
publisher.none.fl_str_mv Crimson Publishers
dc.source.none.fl_str_mv Modern Concepts & Developments in Agronomy 7 (2) : 700-711 (Agosto 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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