Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina

Autores
Kin, Marta Susana; Fort, Marcelo Cristian; Torioni, Susana Marta; Casanave, Emma Beatriz
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Brucellosis is a zoonotic disease transmitted from an animal reservoir to humans. Both, wildlife and domestic animals, contribute to the spreading of these zoonosis. The surveillance of the animal health status is strictly regulated for domestic animals, whereas disease monitoring in wildlife does not exist. The aim of the present study was to provide data on the prevalence of anti-Brucella antibodies in Chaetophractus villosus from a region of La Pampa, Argentina to assess public health risks. The C. villosus is endemic to South America, and in Argentina it represents a food resource for human consumption. A total of 150 sera of armadillos bleeding between 2007 and 2010 were tested using buffered plate antigen test (BPAT), serum agglutination test (SAT), 2-mercaptoethanol (2-ME) and complement fixation test (CFT), for the detection of anti-Brucella antibodies. Antibodies to Brucella sp. were found in 16% (24:150) of the armadillos tested using the BPAT test. All 24 positive samples were confirmed by the SAT, 2-ME and CFT tests. Strain isolation was attempted from liver and spleen samples of two animals with positive serology. Isolates were characterized by conventional biotyping and identification of specific DNA using polymerase chain reaction (PCR). A total of 2 isolates were recovered from spleen and liver. Both of them were identified as Brucella suis biovar 1. This preliminary study provides the first report on the seroprevalence of brucellosis and describes the first isolate of B. suis biovar 1 in C. villosus in Argentina.
EEA Anguil
Fil: Kin, Marta Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fort, Marcelo Cristian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Torioni, Susana Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Casanave, Emma Beatriz. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico Bahia Blanca. Instituto de Ciencias Biologicas y Biomedicas del Sur; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fuente
Veterinary microbiology 170 (3–4) : 442-445. (4 June 2014)
Materia
Brucella Suis
Armadillo
Xenarthra
zoonosis
Anticuerpos
Zoonoses
Antibodies
Chaetophractus Villosus
La Pampa
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3847
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3847
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network_name_str INTA Digital (INTA)
spelling Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, ArgentinaKin, Marta SusanaFort, Marcelo CristianTorioni, Susana MartaCasanave, Emma BeatrizBrucella SuisArmadilloXenarthrazoonosisAnticuerposZoonosesAntibodiesChaetophractus VillosusLa PampaBrucellosis is a zoonotic disease transmitted from an animal reservoir to humans. Both, wildlife and domestic animals, contribute to the spreading of these zoonosis. The surveillance of the animal health status is strictly regulated for domestic animals, whereas disease monitoring in wildlife does not exist. The aim of the present study was to provide data on the prevalence of anti-Brucella antibodies in Chaetophractus villosus from a region of La Pampa, Argentina to assess public health risks. The C. villosus is endemic to South America, and in Argentina it represents a food resource for human consumption. A total of 150 sera of armadillos bleeding between 2007 and 2010 were tested using buffered plate antigen test (BPAT), serum agglutination test (SAT), 2-mercaptoethanol (2-ME) and complement fixation test (CFT), for the detection of anti-Brucella antibodies. Antibodies to Brucella sp. were found in 16% (24:150) of the armadillos tested using the BPAT test. All 24 positive samples were confirmed by the SAT, 2-ME and CFT tests. Strain isolation was attempted from liver and spleen samples of two animals with positive serology. Isolates were characterized by conventional biotyping and identification of specific DNA using polymerase chain reaction (PCR). A total of 2 isolates were recovered from spleen and liver. Both of them were identified as Brucella suis biovar 1. This preliminary study provides the first report on the seroprevalence of brucellosis and describes the first isolate of B. suis biovar 1 in C. villosus in Argentina.EEA AnguilFil: Kin, Marta Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fort, Marcelo Cristian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; ArgentinaFil: Torioni, Susana Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Casanave, Emma Beatriz. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico Bahia Blanca. Instituto de Ciencias Biologicas y Biomedicas del Sur; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaElsevier2018-11-09T13:47:03Z2018-11-09T13:47:03Z2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3847https://www.sciencedirect.com/science/article/pii/S0378113514000741?via%3Dihub0378-1135https://doi.org/10.1016/j.vetmic.2014.01.039Veterinary microbiology 170 (3–4) : 442-445. (4 June 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengLa Pampa (province)info:eu-repo/semantics/restrictedAccess2025-09-29T13:44:29Zoai:localhost:20.500.12123/3847instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:30.061INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina
title Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina
spellingShingle Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina
Kin, Marta Susana
Brucella Suis
Armadillo
Xenarthra
zoonosis
Anticuerpos
Zoonoses
Antibodies
Chaetophractus Villosus
La Pampa
title_short Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina
title_full Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina
title_fullStr Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina
title_full_unstemmed Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina
title_sort Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina
dc.creator.none.fl_str_mv Kin, Marta Susana
Fort, Marcelo Cristian
Torioni, Susana Marta
Casanave, Emma Beatriz
author Kin, Marta Susana
author_facet Kin, Marta Susana
Fort, Marcelo Cristian
Torioni, Susana Marta
Casanave, Emma Beatriz
author_role author
author2 Fort, Marcelo Cristian
Torioni, Susana Marta
Casanave, Emma Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv Brucella Suis
Armadillo
Xenarthra
zoonosis
Anticuerpos
Zoonoses
Antibodies
Chaetophractus Villosus
La Pampa
topic Brucella Suis
Armadillo
Xenarthra
zoonosis
Anticuerpos
Zoonoses
Antibodies
Chaetophractus Villosus
La Pampa
dc.description.none.fl_txt_mv Brucellosis is a zoonotic disease transmitted from an animal reservoir to humans. Both, wildlife and domestic animals, contribute to the spreading of these zoonosis. The surveillance of the animal health status is strictly regulated for domestic animals, whereas disease monitoring in wildlife does not exist. The aim of the present study was to provide data on the prevalence of anti-Brucella antibodies in Chaetophractus villosus from a region of La Pampa, Argentina to assess public health risks. The C. villosus is endemic to South America, and in Argentina it represents a food resource for human consumption. A total of 150 sera of armadillos bleeding between 2007 and 2010 were tested using buffered plate antigen test (BPAT), serum agglutination test (SAT), 2-mercaptoethanol (2-ME) and complement fixation test (CFT), for the detection of anti-Brucella antibodies. Antibodies to Brucella sp. were found in 16% (24:150) of the armadillos tested using the BPAT test. All 24 positive samples were confirmed by the SAT, 2-ME and CFT tests. Strain isolation was attempted from liver and spleen samples of two animals with positive serology. Isolates were characterized by conventional biotyping and identification of specific DNA using polymerase chain reaction (PCR). A total of 2 isolates were recovered from spleen and liver. Both of them were identified as Brucella suis biovar 1. This preliminary study provides the first report on the seroprevalence of brucellosis and describes the first isolate of B. suis biovar 1 in C. villosus in Argentina.
EEA Anguil
Fil: Kin, Marta Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Fort, Marcelo Cristian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Torioni, Susana Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Casanave, Emma Beatriz. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico Bahia Blanca. Instituto de Ciencias Biologicas y Biomedicas del Sur; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
description Brucellosis is a zoonotic disease transmitted from an animal reservoir to humans. Both, wildlife and domestic animals, contribute to the spreading of these zoonosis. The surveillance of the animal health status is strictly regulated for domestic animals, whereas disease monitoring in wildlife does not exist. The aim of the present study was to provide data on the prevalence of anti-Brucella antibodies in Chaetophractus villosus from a region of La Pampa, Argentina to assess public health risks. The C. villosus is endemic to South America, and in Argentina it represents a food resource for human consumption. A total of 150 sera of armadillos bleeding between 2007 and 2010 were tested using buffered plate antigen test (BPAT), serum agglutination test (SAT), 2-mercaptoethanol (2-ME) and complement fixation test (CFT), for the detection of anti-Brucella antibodies. Antibodies to Brucella sp. were found in 16% (24:150) of the armadillos tested using the BPAT test. All 24 positive samples were confirmed by the SAT, 2-ME and CFT tests. Strain isolation was attempted from liver and spleen samples of two animals with positive serology. Isolates were characterized by conventional biotyping and identification of specific DNA using polymerase chain reaction (PCR). A total of 2 isolates were recovered from spleen and liver. Both of them were identified as Brucella suis biovar 1. This preliminary study provides the first report on the seroprevalence of brucellosis and describes the first isolate of B. suis biovar 1 in C. villosus in Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
2018-11-09T13:47:03Z
2018-11-09T13:47:03Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3847
https://www.sciencedirect.com/science/article/pii/S0378113514000741?via%3Dihub
0378-1135
https://doi.org/10.1016/j.vetmic.2014.01.039
url http://hdl.handle.net/20.500.12123/3847
https://www.sciencedirect.com/science/article/pii/S0378113514000741?via%3Dihub
https://doi.org/10.1016/j.vetmic.2014.01.039
identifier_str_mv 0378-1135
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv La Pampa (province)
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary microbiology 170 (3–4) : 442-445. (4 June 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619128090918912
score 12.891075

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