Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina

Autores
Bernanrdi Lima, Nelson; Conforto, Erica Cinthia; Serri, Dannae Lilia; Campilongo Mancilla, Ernesto Javier; Stenglein, Sebastian; Vargas Gil, Silvina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Alfalfa is an important forage crop in Argentina, with a large area dedicated to its cultivation. However, plant diseases are a common cause of loss because they limit productivity and reduce forage quality. Anthracnose, caused by the Colletotrichum species, is one of the most important diseases affecting alfalfa worldwide. In July 2018, alfalfa plants with anthracnose symptoms were collected in commercial fields of Cordoba province, Argentina. Initially, infected plants showed small, pale gray lesions on stems, which turned oval and developed irregular dark brown/black borders as they enlarged. Three fungal isolates were obtained from the necrotic tissues of stems and single-spore purified. Based on the morphology and multilocus phylogenetic analysis, combined sequences of the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H3 (HIS3) and actin (ACT) genes, the three isolates were identified as Colletotrichum americae-borealis. Pathogenicity testing showed these isolates were pathogenic to alfalfa and Koch's postulates were verified by re-isolating the pathogen.
Instituto de Patología Vegetal
Fil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bernardi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conforto, Erica Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Serri, Dannae Lilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Serri, Dannae Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Campilongo Mancilla, Ernesto Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Campilongo Mancilla, Ernesto Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Stenglein, Sebastian. Laboratorio de Biología Functional y Biotecnología (BIOLAB). Unidad Ejecutora Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC); Argentina
Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fuente
Crop Protection 148 : 105732 ( 2021)
Materia
Medicago Sativa
Colletotrichum
Argentina
Anthracnosis
Phylogeny
Filogenia
Antracnosis
Molecular Phylogeny
Plant Pathogen
Alfalfa
Lucerne
Córdoba, Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9785
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9785
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, ArgentinaBernanrdi Lima, NelsonConforto, Erica CinthiaSerri, Dannae LiliaCampilongo Mancilla, Ernesto JavierStenglein, SebastianVargas Gil, SilvinaMedicago SativaColletotrichumArgentinaAnthracnosisPhylogenyFilogeniaAntracnosisMolecular PhylogenyPlant PathogenAlfalfaLucerneCórdoba, ArgentinaAlfalfa is an important forage crop in Argentina, with a large area dedicated to its cultivation. However, plant diseases are a common cause of loss because they limit productivity and reduce forage quality. Anthracnose, caused by the Colletotrichum species, is one of the most important diseases affecting alfalfa worldwide. In July 2018, alfalfa plants with anthracnose symptoms were collected in commercial fields of Cordoba province, Argentina. Initially, infected plants showed small, pale gray lesions on stems, which turned oval and developed irregular dark brown/black borders as they enlarged. Three fungal isolates were obtained from the necrotic tissues of stems and single-spore purified. Based on the morphology and multilocus phylogenetic analysis, combined sequences of the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H3 (HIS3) and actin (ACT) genes, the three isolates were identified as Colletotrichum americae-borealis. Pathogenicity testing showed these isolates were pathogenic to alfalfa and Koch's postulates were verified by re-isolating the pathogen.Instituto de Patología VegetalFil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Bernardi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Conforto, Erica Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Serri, Dannae Lilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Serri, Dannae Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Campilongo Mancilla, Ernesto Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Campilongo Mancilla, Ernesto Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Stenglein, Sebastian. Laboratorio de Biología Functional y Biotecnología (BIOLAB). Unidad Ejecutora Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC); ArgentinaFil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaElsevier2021-07-12T16:43:02Z2021-07-12T16:43:02Z2021-06-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9785https://www.sciencedirect.com/science/article/pii/S02612194210020270261-2194https://doi.org/10.1016/j.cropro.2021.105732Crop Protection 148 : 105732 ( 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I090-001/2019-PD-E4-I090-001/AR./Análisis de patosistemas en cultivos agrícolas y especies forestales. Caracterización de sus componentesCórdoba .......... (province) (World, South America, Argentina)1001169info:eu-repo/semantics/restrictedAccess2025-10-16T09:30:09Zoai:localhost:20.500.12123/9785instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:09.591INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
title Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
spellingShingle Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
Bernanrdi Lima, Nelson
Medicago Sativa
Colletotrichum
Argentina
Anthracnosis
Phylogeny
Filogenia
Antracnosis
Molecular Phylogeny
Plant Pathogen
Alfalfa
Lucerne
Córdoba, Argentina
title_short Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
title_full Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
title_fullStr Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
title_full_unstemmed Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
title_sort Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
dc.creator.none.fl_str_mv Bernanrdi Lima, Nelson
Conforto, Erica Cinthia
Serri, Dannae Lilia
Campilongo Mancilla, Ernesto Javier
Stenglein, Sebastian
Vargas Gil, Silvina
author Bernanrdi Lima, Nelson
author_facet Bernanrdi Lima, Nelson
Conforto, Erica Cinthia
Serri, Dannae Lilia
Campilongo Mancilla, Ernesto Javier
Stenglein, Sebastian
Vargas Gil, Silvina
author_role author
author2 Conforto, Erica Cinthia
Serri, Dannae Lilia
Campilongo Mancilla, Ernesto Javier
Stenglein, Sebastian
Vargas Gil, Silvina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Medicago Sativa
Colletotrichum
Argentina
Anthracnosis
Phylogeny
Filogenia
Antracnosis
Molecular Phylogeny
Plant Pathogen
Alfalfa
Lucerne
Córdoba, Argentina
topic Medicago Sativa
Colletotrichum
Argentina
Anthracnosis
Phylogeny
Filogenia
Antracnosis
Molecular Phylogeny
Plant Pathogen
Alfalfa
Lucerne
Córdoba, Argentina
dc.description.none.fl_txt_mv Alfalfa is an important forage crop in Argentina, with a large area dedicated to its cultivation. However, plant diseases are a common cause of loss because they limit productivity and reduce forage quality. Anthracnose, caused by the Colletotrichum species, is one of the most important diseases affecting alfalfa worldwide. In July 2018, alfalfa plants with anthracnose symptoms were collected in commercial fields of Cordoba province, Argentina. Initially, infected plants showed small, pale gray lesions on stems, which turned oval and developed irregular dark brown/black borders as they enlarged. Three fungal isolates were obtained from the necrotic tissues of stems and single-spore purified. Based on the morphology and multilocus phylogenetic analysis, combined sequences of the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H3 (HIS3) and actin (ACT) genes, the three isolates were identified as Colletotrichum americae-borealis. Pathogenicity testing showed these isolates were pathogenic to alfalfa and Koch's postulates were verified by re-isolating the pathogen.
Instituto de Patología Vegetal
Fil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bernardi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conforto, Erica Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Serri, Dannae Lilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Serri, Dannae Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Campilongo Mancilla, Ernesto Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Campilongo Mancilla, Ernesto Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Stenglein, Sebastian. Laboratorio de Biología Functional y Biotecnología (BIOLAB). Unidad Ejecutora Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC); Argentina
Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
description Alfalfa is an important forage crop in Argentina, with a large area dedicated to its cultivation. However, plant diseases are a common cause of loss because they limit productivity and reduce forage quality. Anthracnose, caused by the Colletotrichum species, is one of the most important diseases affecting alfalfa worldwide. In July 2018, alfalfa plants with anthracnose symptoms were collected in commercial fields of Cordoba province, Argentina. Initially, infected plants showed small, pale gray lesions on stems, which turned oval and developed irregular dark brown/black borders as they enlarged. Three fungal isolates were obtained from the necrotic tissues of stems and single-spore purified. Based on the morphology and multilocus phylogenetic analysis, combined sequences of the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H3 (HIS3) and actin (ACT) genes, the three isolates were identified as Colletotrichum americae-borealis. Pathogenicity testing showed these isolates were pathogenic to alfalfa and Koch's postulates were verified by re-isolating the pathogen.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-12T16:43:02Z
2021-07-12T16:43:02Z
2021-06-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9785
https://www.sciencedirect.com/science/article/pii/S0261219421002027
0261-2194
https://doi.org/10.1016/j.cropro.2021.105732
url http://hdl.handle.net/20.500.12123/9785
https://www.sciencedirect.com/science/article/pii/S0261219421002027
https://doi.org/10.1016/j.cropro.2021.105732
identifier_str_mv 0261-2194
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E4-I090-001/2019-PD-E4-I090-001/AR./Análisis de patosistemas en cultivos agrícolas y especies forestales. Caracterización de sus componentes
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Córdoba .......... (province) (World, South America, Argentina)
1001169
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Crop Protection 148 : 105732 ( 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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