First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina

Autores
Sebastian, Patrick; Vaschalde, Paula Josefina; Pelosi, María Celeste; Flores, Fernando Sebastián; Yedro, Julio; Tarragona, Evelina Luisa
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In November 2022, a male specimen of Subulo gouazoubira was found with signs of obnubilation and recumbency without the ability to maintain sternal or ambulatory posture. Based on the symptoms, serological, biochemical and molecular analyses were performed. The results of the biochemical blood analysis and the clinical signs were compatible with theileriosis. DNA of Theileria were detected in the blood sample by PCR analysis. The phylogenetic analysis of the obtained partial sequences of the 18S rDNA gen resulted in the identification of the detected strain as a Theileria genospecies closely related to Theileria spp. detected in other deer species. Further, a genospecies of Anaplasma was detected in the blood sample. This genospecies is located phylogenetically near to Anaplasma phagocytophilum. The results of this study demonstrate co-infection with two novel genospecies of Anaplasma and Theileria in a clinical case of a free-living S. gouazoubira for the first time in Argentina. However, the pathogenicity of these strains and specific role of S. gouazoubira in their enzootic transmission cycles remains unclear. To improve epidemiological understanding, assess risks and develop targeted control strategies, future studies should prioritize the isolation, characterization and cultivation of these genospecies and the assessment of vector competence.
EEA Rafaela
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Vaschalde, Paula J. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina
Fil: Vaschalde, Paula J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina
Fil: Pelosi, María Celeste. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Zoología, Diversidad y Ambiente; Argentina
Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba (CIEC); Argentina
Fil: Flores, Fernando Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Yedro, Julio. Gobierno de la Provincia de Entre Ríos. Dirección de Recursos Naturales; Argentina
Fil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fuente
Wild 2 (3) : 35. (September 2025)
Materia
Theileria
Anaplasma
Cervidae
Ticks
Identification
Entre Rios
Garrapata
Identificación
Subulo gouazoubira
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24056
Acceder
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oai_identifier_str oai:localhost:20.500.12123/24056
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, ArgentinaSebastian, PatrickVaschalde, Paula JosefinaPelosi, María CelesteFlores, Fernando SebastiánYedro, JulioTarragona, Evelina LuisaTheileriaAnaplasmaCervidaeTicksIdentificationEntre RiosGarrapataIdentificaciónSubulo gouazoubiraIn November 2022, a male specimen of Subulo gouazoubira was found with signs of obnubilation and recumbency without the ability to maintain sternal or ambulatory posture. Based on the symptoms, serological, biochemical and molecular analyses were performed. The results of the biochemical blood analysis and the clinical signs were compatible with theileriosis. DNA of Theileria were detected in the blood sample by PCR analysis. The phylogenetic analysis of the obtained partial sequences of the 18S rDNA gen resulted in the identification of the detected strain as a Theileria genospecies closely related to Theileria spp. detected in other deer species. Further, a genospecies of Anaplasma was detected in the blood sample. This genospecies is located phylogenetically near to Anaplasma phagocytophilum. The results of this study demonstrate co-infection with two novel genospecies of Anaplasma and Theileria in a clinical case of a free-living S. gouazoubira for the first time in Argentina. However, the pathogenicity of these strains and specific role of S. gouazoubira in their enzootic transmission cycles remains unclear. To improve epidemiological understanding, assess risks and develop targeted control strategies, future studies should prioritize the isolation, characterization and cultivation of these genospecies and the assessment of vector competence.EEA RafaelaFil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Vaschalde, Paula J. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; ArgentinaFil: Vaschalde, Paula J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; ArgentinaFil: Pelosi, María Celeste. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Zoología, Diversidad y Ambiente; ArgentinaFil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba (CIEC); ArgentinaFil: Flores, Fernando Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Yedro, Julio. Gobierno de la Provincia de Entre Ríos. Dirección de Recursos Naturales; ArgentinaFil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaMDPI2025-10-03T12:03:18Z2025-10-03T12:03:18Z2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24056https://www.mdpi.com/3042-4526/2/3/353042-4526https://doi.org/10.3390/wild2030035Wild 2 (3) : 35. (September 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PE-L01-I044, Enfermedades subtropicales con impacto sobre la producción, salud pública y sustentabilidad en la ganadería del norte de Argentinainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:32:37Zoai:localhost:20.500.12123/24056instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:32:38.171INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
title First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
spellingShingle First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
Sebastian, Patrick
Theileria
Anaplasma
Cervidae
Ticks
Identification
Entre Rios
Garrapata
Identificación
Subulo gouazoubira
title_short First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
title_full First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
title_fullStr First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
title_full_unstemmed First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
title_sort First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
dc.creator.none.fl_str_mv Sebastian, Patrick
Vaschalde, Paula Josefina
Pelosi, María Celeste
Flores, Fernando Sebastián
Yedro, Julio
Tarragona, Evelina Luisa
author Sebastian, Patrick
author_facet Sebastian, Patrick
Vaschalde, Paula Josefina
Pelosi, María Celeste
Flores, Fernando Sebastián
Yedro, Julio
Tarragona, Evelina Luisa
author_role author
author2 Vaschalde, Paula Josefina
Pelosi, María Celeste
Flores, Fernando Sebastián
Yedro, Julio
Tarragona, Evelina Luisa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Theileria
Anaplasma
Cervidae
Ticks
Identification
Entre Rios
Garrapata
Identificación
Subulo gouazoubira
topic Theileria
Anaplasma
Cervidae
Ticks
Identification
Entre Rios
Garrapata
Identificación
Subulo gouazoubira
dc.description.none.fl_txt_mv In November 2022, a male specimen of Subulo gouazoubira was found with signs of obnubilation and recumbency without the ability to maintain sternal or ambulatory posture. Based on the symptoms, serological, biochemical and molecular analyses were performed. The results of the biochemical blood analysis and the clinical signs were compatible with theileriosis. DNA of Theileria were detected in the blood sample by PCR analysis. The phylogenetic analysis of the obtained partial sequences of the 18S rDNA gen resulted in the identification of the detected strain as a Theileria genospecies closely related to Theileria spp. detected in other deer species. Further, a genospecies of Anaplasma was detected in the blood sample. This genospecies is located phylogenetically near to Anaplasma phagocytophilum. The results of this study demonstrate co-infection with two novel genospecies of Anaplasma and Theileria in a clinical case of a free-living S. gouazoubira for the first time in Argentina. However, the pathogenicity of these strains and specific role of S. gouazoubira in their enzootic transmission cycles remains unclear. To improve epidemiological understanding, assess risks and develop targeted control strategies, future studies should prioritize the isolation, characterization and cultivation of these genospecies and the assessment of vector competence.
EEA Rafaela
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Vaschalde, Paula J. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina
Fil: Vaschalde, Paula J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina
Fil: Pelosi, María Celeste. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Zoología, Diversidad y Ambiente; Argentina
Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba (CIEC); Argentina
Fil: Flores, Fernando Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Yedro, Julio. Gobierno de la Provincia de Entre Ríos. Dirección de Recursos Naturales; Argentina
Fil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
description In November 2022, a male specimen of Subulo gouazoubira was found with signs of obnubilation and recumbency without the ability to maintain sternal or ambulatory posture. Based on the symptoms, serological, biochemical and molecular analyses were performed. The results of the biochemical blood analysis and the clinical signs were compatible with theileriosis. DNA of Theileria were detected in the blood sample by PCR analysis. The phylogenetic analysis of the obtained partial sequences of the 18S rDNA gen resulted in the identification of the detected strain as a Theileria genospecies closely related to Theileria spp. detected in other deer species. Further, a genospecies of Anaplasma was detected in the blood sample. This genospecies is located phylogenetically near to Anaplasma phagocytophilum. The results of this study demonstrate co-infection with two novel genospecies of Anaplasma and Theileria in a clinical case of a free-living S. gouazoubira for the first time in Argentina. However, the pathogenicity of these strains and specific role of S. gouazoubira in their enzootic transmission cycles remains unclear. To improve epidemiological understanding, assess risks and develop targeted control strategies, future studies should prioritize the isolation, characterization and cultivation of these genospecies and the assessment of vector competence.
publishDate 2025
dc.date.none.fl_str_mv 2025-10-03T12:03:18Z
2025-10-03T12:03:18Z
2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24056
https://www.mdpi.com/3042-4526/2/3/35
3042-4526
https://doi.org/10.3390/wild2030035
url http://hdl.handle.net/20.500.12123/24056
https://www.mdpi.com/3042-4526/2/3/35
https://doi.org/10.3390/wild2030035
identifier_str_mv 3042-4526
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PE-L01-I044, Enfermedades subtropicales con impacto sobre la producción, salud pública y sustentabilidad en la ganadería del norte de Argentina
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Wild 2 (3) : 35. (September 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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