Interaction between natural magnetite sub-micrometric particles and the Fasciola hepatica egg: the role of the exposed surface area

Autores
Raineri, Mariana; Lima Jr., Enio; Larroza, Marcela Patricia; Moreno, Mario Sergio; Vásquez Mansilla, Marcelo; Pappalardo, Juan Sebastián; Zysler, Roberto Daniel
Año de publicación
2019
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fasciolosis is a zoonotic world widely distributed disease caused by the liver fluke Fasciola hepatica, which affects animals and occasionally humans. On the other hand, natural iron oxide particles like magnetite are commonly found in soils where they participate in a wide range of environmental processes like organic matter decomposition, the adsorption of ions and molecules, and chemical reactions that involve the participation of soil living microorganisms. Since Fasciola eggs become soil components after being released with the infected animal faeces, this study focused on the characterization of the natural interaction between natural sub-micrometric magnetite particles and F. hepatica eggs. Our results indicate that particle binding to the F. hepatica egg depends on the particle size and it is also related to the exposed surface area since any condition that favors particle agglomeration leads to the reduction of the particle-eggshell binding intensity. Interestingly, this binding was avoided when proteins or phosphate were incorporated to the incubation solution, but not after formaldehyde fixation of eggs. Finally, when eggs were exposed to an external magnet after being incubated with magnetite particles, they were attracted to it without particles being detached, indicating a strong type of bonding between them. Therefore, the results presented here give new insights in order to improve the possibility of harvesting F. hepatica eggs by using magnetic materials.
Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo de Salud Animal
Fil: Raineri, Mariana. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lima Jr., Enio. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Larroza, Marcela Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; Argentina
Fil: Moreno, Mario Sergio. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vásquez Mansilla, Marcelo. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; Argentina
Fil: Zysler, Roberto Daniel. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Experimental Parasitology 199 : 59–66 (2019)
Materia
Fasciola Hepática
Enfermedades Bacterianas
Enfermedades de los Animales
Bacterial Diseases
Animal Diseases
Fasciolosis
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4754
Acceder
id INTADig_03febf6a7cefa851c82726269505e7e4
oai_identifier_str oai:localhost:20.500.12123/4754
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Interaction between natural magnetite sub-micrometric particles and the Fasciola hepatica egg: the role of the exposed surface areaRaineri, MarianaLima Jr., EnioLarroza, Marcela PatriciaMoreno, Mario SergioVásquez Mansilla, MarceloPappalardo, Juan SebastiánZysler, Roberto DanielFasciola HepáticaEnfermedades BacterianasEnfermedades de los AnimalesBacterial DiseasesAnimal DiseasesFasciolosisFasciolosis is a zoonotic world widely distributed disease caused by the liver fluke Fasciola hepatica, which affects animals and occasionally humans. On the other hand, natural iron oxide particles like magnetite are commonly found in soils where they participate in a wide range of environmental processes like organic matter decomposition, the adsorption of ions and molecules, and chemical reactions that involve the participation of soil living microorganisms. Since Fasciola eggs become soil components after being released with the infected animal faeces, this study focused on the characterization of the natural interaction between natural sub-micrometric magnetite particles and F. hepatica eggs. Our results indicate that particle binding to the F. hepatica egg depends on the particle size and it is also related to the exposed surface area since any condition that favors particle agglomeration leads to the reduction of the particle-eggshell binding intensity. Interestingly, this binding was avoided when proteins or phosphate were incorporated to the incubation solution, but not after formaldehyde fixation of eggs. Finally, when eggs were exposed to an external magnet after being incubated with magnetite particles, they were attracted to it without particles being detached, indicating a strong type of bonding between them. Therefore, the results presented here give new insights in order to improve the possibility of harvesting F. hepatica eggs by using magnetic materials.Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo de Salud AnimalFil: Raineri, Mariana. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lima Jr., Enio. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Larroza, Marcela Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; ArgentinaFil: Moreno, Mario Sergio. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vásquez Mansilla, Marcelo. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; ArgentinaFil: Zysler, Roberto Daniel. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-03-27T12:21:46Z2019-03-27T12:21:46Z2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4754https://www.sciencedirect.com/science/article/pii/S00144894183042470014-4894https://doi.org/10.1016/j.exppara.2019.02.006Experimental Parasitology 199 : 59–66 (2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaspainfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:54Zoai:localhost:20.500.12123/4754instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:54.371INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Interaction between natural magnetite sub-micrometric particles and the Fasciola hepatica egg: the role of the exposed surface area
title Interaction between natural magnetite sub-micrometric particles and the Fasciola hepatica egg: the role of the exposed surface area
spellingShingle Interaction between natural magnetite sub-micrometric particles and the Fasciola hepatica egg: the role of the exposed surface area
Raineri, Mariana
Fasciola Hepática
Enfermedades Bacterianas
Enfermedades de los Animales
Bacterial Diseases
Animal Diseases
Fasciolosis
title_short Interaction between natural magnetite sub-micrometric particles and the Fasciola hepatica egg: the role of the exposed surface area
title_full Interaction between natural magnetite sub-micrometric particles and the Fasciola hepatica egg: the role of the exposed surface area
title_fullStr Interaction between natural magnetite sub-micrometric particles and the Fasciola hepatica egg: the role of the exposed surface area
title_full_unstemmed Interaction between natural magnetite sub-micrometric particles and the Fasciola hepatica egg: the role of the exposed surface area
title_sort Interaction between natural magnetite sub-micrometric particles and the Fasciola hepatica egg: the role of the exposed surface area
dc.creator.none.fl_str_mv Raineri, Mariana
Lima Jr., Enio
Larroza, Marcela Patricia
Moreno, Mario Sergio
Vásquez Mansilla, Marcelo
Pappalardo, Juan Sebastián
Zysler, Roberto Daniel
author Raineri, Mariana
author_facet Raineri, Mariana
Lima Jr., Enio
Larroza, Marcela Patricia
Moreno, Mario Sergio
Vásquez Mansilla, Marcelo
Pappalardo, Juan Sebastián
Zysler, Roberto Daniel
author_role author
author2 Lima Jr., Enio
Larroza, Marcela Patricia
Moreno, Mario Sergio
Vásquez Mansilla, Marcelo
Pappalardo, Juan Sebastián
Zysler, Roberto Daniel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Fasciola Hepática
Enfermedades Bacterianas
Enfermedades de los Animales
Bacterial Diseases
Animal Diseases
Fasciolosis
topic Fasciola Hepática
Enfermedades Bacterianas
Enfermedades de los Animales
Bacterial Diseases
Animal Diseases
Fasciolosis
dc.description.none.fl_txt_mv Fasciolosis is a zoonotic world widely distributed disease caused by the liver fluke Fasciola hepatica, which affects animals and occasionally humans. On the other hand, natural iron oxide particles like magnetite are commonly found in soils where they participate in a wide range of environmental processes like organic matter decomposition, the adsorption of ions and molecules, and chemical reactions that involve the participation of soil living microorganisms. Since Fasciola eggs become soil components after being released with the infected animal faeces, this study focused on the characterization of the natural interaction between natural sub-micrometric magnetite particles and F. hepatica eggs. Our results indicate that particle binding to the F. hepatica egg depends on the particle size and it is also related to the exposed surface area since any condition that favors particle agglomeration leads to the reduction of the particle-eggshell binding intensity. Interestingly, this binding was avoided when proteins or phosphate were incorporated to the incubation solution, but not after formaldehyde fixation of eggs. Finally, when eggs were exposed to an external magnet after being incubated with magnetite particles, they were attracted to it without particles being detached, indicating a strong type of bonding between them. Therefore, the results presented here give new insights in order to improve the possibility of harvesting F. hepatica eggs by using magnetic materials.
Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo de Salud Animal
Fil: Raineri, Mariana. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lima Jr., Enio. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Larroza, Marcela Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; Argentina
Fil: Moreno, Mario Sergio. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vásquez Mansilla, Marcelo. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; Argentina
Fil: Zysler, Roberto Daniel. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Instituto de Nanociencia y Nanotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Fasciolosis is a zoonotic world widely distributed disease caused by the liver fluke Fasciola hepatica, which affects animals and occasionally humans. On the other hand, natural iron oxide particles like magnetite are commonly found in soils where they participate in a wide range of environmental processes like organic matter decomposition, the adsorption of ions and molecules, and chemical reactions that involve the participation of soil living microorganisms. Since Fasciola eggs become soil components after being released with the infected animal faeces, this study focused on the characterization of the natural interaction between natural sub-micrometric magnetite particles and F. hepatica eggs. Our results indicate that particle binding to the F. hepatica egg depends on the particle size and it is also related to the exposed surface area since any condition that favors particle agglomeration leads to the reduction of the particle-eggshell binding intensity. Interestingly, this binding was avoided when proteins or phosphate were incorporated to the incubation solution, but not after formaldehyde fixation of eggs. Finally, when eggs were exposed to an external magnet after being incubated with magnetite particles, they were attracted to it without particles being detached, indicating a strong type of bonding between them. Therefore, the results presented here give new insights in order to improve the possibility of harvesting F. hepatica eggs by using magnetic materials.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-27T12:21:46Z
2019-03-27T12:21:46Z
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4754
https://www.sciencedirect.com/science/article/pii/S0014489418304247
0014-4894
https://doi.org/10.1016/j.exppara.2019.02.006
url http://hdl.handle.net/20.500.12123/4754
https://www.sciencedirect.com/science/article/pii/S0014489418304247
https://doi.org/10.1016/j.exppara.2019.02.006
identifier_str_mv 0014-4894
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Experimental Parasitology 199 : 59–66 (2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341364537229312
score 12.623145

Publicaciones similares