Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population

Autores
Quero, Gastón; Gutíerrez, Lucía; Lascano, Hernan Ramiro; Monza, Jorge; Sandal, Niels; Borsani, Omar
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genus Lotus includes a group of forage legume species including genotypes of agronomic interest and model species. In this work, an experimental hydroponic growth system allowed the discrimination of growth responses to ionic–osmotic stress in a population of recombinant inbred lines (RILs) developed from L. japonicus × L. burttii and the identification of the associated quantitative trait loci (QTLs). The analyses led to the identification of eight QTLs: three for shoot growth localised on chromosome 3, 5 and 6; one for root growth on chromosome 1; three for total growth on chromosome 1, 4 and 5; and one associated with shoot/root ratio on chromosome 3. An interaction of QTL × stress condition was established and the effect of the environment quantified. In summary, it was established that the allele from L. burttii explained most responses to osmotic stress, while the alleles of L. japonicus explained the responses related to ionic stress conditions. Of 49 markers linked to all QTLs identified, 41 expressed superiority of the L. burttii parental allele in the osmotic stress condition, but when an iso-osmotic concentration of NaCl was applied, L. burttii lost superiority in 21 of these markers. This shows the superiority of the L. japonicus parental allele in ionic stress conditions. This study is the first report in which a RIL population of lotus is analysed with the aim of providing molecular markers associated with plant responses to ionic or osmotic stress.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Quero, Gastón. Universidad de la República. Facultad de Agronomía. Laboratorio de Bioquímica; Uruguay
Fil: Gutíerrez, Lucía. Universidad de la República. Facultad de Agronomía. Departamento de Biometría, Estadística y Computación; Uruguay
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Monza, Jorge. Universidad de la República. Facultad de Agronomía. Laboratorio de Bioquímica; Uruguay
Fil: Sandal, Niels. Aarhus University. Centre for Carbohydrate Recognition and Signalling. Department of Molecular Biology and Genetics; Dinamarca
Fil: Borsani, Omar. Universidad de la República. Facultad de Agronomía. Laboratorio de Bioquímica; Uruguay
Fuente
Crop and Pasture Science 65 (2) : 139-149 (2014)
Materia
Hydroponics
Osmotic Stress
Estrés Osmótico
Cultivo Hidropónico
Hydroponic Growth
PEG Stress
Salt Stress
Lotus japonicus
Lotus burttii
Estrés Salino
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10936
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10936
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network_name_str INTA Digital (INTA)
spelling Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL populationQuero, GastónGutíerrez, LucíaLascano, Hernan RamiroMonza, JorgeSandal, NielsBorsani, OmarHydroponicsOsmotic StressEstrés OsmóticoCultivo HidropónicoHydroponic GrowthPEG StressSalt StressLotus japonicusLotus burttiiEstrés SalinoThe genus Lotus includes a group of forage legume species including genotypes of agronomic interest and model species. In this work, an experimental hydroponic growth system allowed the discrimination of growth responses to ionic–osmotic stress in a population of recombinant inbred lines (RILs) developed from L. japonicus × L. burttii and the identification of the associated quantitative trait loci (QTLs). The analyses led to the identification of eight QTLs: three for shoot growth localised on chromosome 3, 5 and 6; one for root growth on chromosome 1; three for total growth on chromosome 1, 4 and 5; and one associated with shoot/root ratio on chromosome 3. An interaction of QTL × stress condition was established and the effect of the environment quantified. In summary, it was established that the allele from L. burttii explained most responses to osmotic stress, while the alleles of L. japonicus explained the responses related to ionic stress conditions. Of 49 markers linked to all QTLs identified, 41 expressed superiority of the L. burttii parental allele in the osmotic stress condition, but when an iso-osmotic concentration of NaCl was applied, L. burttii lost superiority in 21 of these markers. This shows the superiority of the L. japonicus parental allele in ionic stress conditions. This study is the first report in which a RIL population of lotus is analysed with the aim of providing molecular markers associated with plant responses to ionic or osmotic stress.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Quero, Gastón. Universidad de la República. Facultad de Agronomía. Laboratorio de Bioquímica; UruguayFil: Gutíerrez, Lucía. Universidad de la República. Facultad de Agronomía. Departamento de Biometría, Estadística y Computación; UruguayFil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Monza, Jorge. Universidad de la República. Facultad de Agronomía. Laboratorio de Bioquímica; UruguayFil: Sandal, Niels. Aarhus University. Centre for Carbohydrate Recognition and Signalling. Department of Molecular Biology and Genetics; DinamarcaFil: Borsani, Omar. Universidad de la República. Facultad de Agronomía. Laboratorio de Bioquímica; UruguayCSIRO Publishing2021-12-17T19:58:38Z2021-12-17T19:58:38Z2014-02-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10936https://www.publish.csiro.au/cp/CP132221836-09471836-5795 (online)https://doi.org/10.1071/CP13222Crop and Pasture Science 65 (2) : 139-149 (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:12Zoai:localhost:20.500.12123/10936instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:13.069INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population
title Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population
spellingShingle Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population
Quero, Gastón
Hydroponics
Osmotic Stress
Estrés Osmótico
Cultivo Hidropónico
Hydroponic Growth
PEG Stress
Salt Stress
Lotus japonicus
Lotus burttii
Estrés Salino
title_short Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population
title_full Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population
title_fullStr Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population
title_full_unstemmed Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population
title_sort Identification of QTLs for shoot and root growth under ionic–osmotic stress in Lotus, using a RIL population
dc.creator.none.fl_str_mv Quero, Gastón
Gutíerrez, Lucía
Lascano, Hernan Ramiro
Monza, Jorge
Sandal, Niels
Borsani, Omar
author Quero, Gastón
author_facet Quero, Gastón
Gutíerrez, Lucía
Lascano, Hernan Ramiro
Monza, Jorge
Sandal, Niels
Borsani, Omar
author_role author
author2 Gutíerrez, Lucía
Lascano, Hernan Ramiro
Monza, Jorge
Sandal, Niels
Borsani, Omar
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Hydroponics
Osmotic Stress
Estrés Osmótico
Cultivo Hidropónico
Hydroponic Growth
PEG Stress
Salt Stress
Lotus japonicus
Lotus burttii
Estrés Salino
topic Hydroponics
Osmotic Stress
Estrés Osmótico
Cultivo Hidropónico
Hydroponic Growth
PEG Stress
Salt Stress
Lotus japonicus
Lotus burttii
Estrés Salino
dc.description.none.fl_txt_mv The genus Lotus includes a group of forage legume species including genotypes of agronomic interest and model species. In this work, an experimental hydroponic growth system allowed the discrimination of growth responses to ionic–osmotic stress in a population of recombinant inbred lines (RILs) developed from L. japonicus × L. burttii and the identification of the associated quantitative trait loci (QTLs). The analyses led to the identification of eight QTLs: three for shoot growth localised on chromosome 3, 5 and 6; one for root growth on chromosome 1; three for total growth on chromosome 1, 4 and 5; and one associated with shoot/root ratio on chromosome 3. An interaction of QTL × stress condition was established and the effect of the environment quantified. In summary, it was established that the allele from L. burttii explained most responses to osmotic stress, while the alleles of L. japonicus explained the responses related to ionic stress conditions. Of 49 markers linked to all QTLs identified, 41 expressed superiority of the L. burttii parental allele in the osmotic stress condition, but when an iso-osmotic concentration of NaCl was applied, L. burttii lost superiority in 21 of these markers. This shows the superiority of the L. japonicus parental allele in ionic stress conditions. This study is the first report in which a RIL population of lotus is analysed with the aim of providing molecular markers associated with plant responses to ionic or osmotic stress.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Quero, Gastón. Universidad de la República. Facultad de Agronomía. Laboratorio de Bioquímica; Uruguay
Fil: Gutíerrez, Lucía. Universidad de la República. Facultad de Agronomía. Departamento de Biometría, Estadística y Computación; Uruguay
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Monza, Jorge. Universidad de la República. Facultad de Agronomía. Laboratorio de Bioquímica; Uruguay
Fil: Sandal, Niels. Aarhus University. Centre for Carbohydrate Recognition and Signalling. Department of Molecular Biology and Genetics; Dinamarca
Fil: Borsani, Omar. Universidad de la República. Facultad de Agronomía. Laboratorio de Bioquímica; Uruguay
description The genus Lotus includes a group of forage legume species including genotypes of agronomic interest and model species. In this work, an experimental hydroponic growth system allowed the discrimination of growth responses to ionic–osmotic stress in a population of recombinant inbred lines (RILs) developed from L. japonicus × L. burttii and the identification of the associated quantitative trait loci (QTLs). The analyses led to the identification of eight QTLs: three for shoot growth localised on chromosome 3, 5 and 6; one for root growth on chromosome 1; three for total growth on chromosome 1, 4 and 5; and one associated with shoot/root ratio on chromosome 3. An interaction of QTL × stress condition was established and the effect of the environment quantified. In summary, it was established that the allele from L. burttii explained most responses to osmotic stress, while the alleles of L. japonicus explained the responses related to ionic stress conditions. Of 49 markers linked to all QTLs identified, 41 expressed superiority of the L. burttii parental allele in the osmotic stress condition, but when an iso-osmotic concentration of NaCl was applied, L. burttii lost superiority in 21 of these markers. This shows the superiority of the L. japonicus parental allele in ionic stress conditions. This study is the first report in which a RIL population of lotus is analysed with the aim of providing molecular markers associated with plant responses to ionic or osmotic stress.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-20
2021-12-17T19:58:38Z
2021-12-17T19:58:38Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10936
https://www.publish.csiro.au/cp/CP13222
1836-0947
1836-5795 (online)
https://doi.org/10.1071/CP13222
url http://hdl.handle.net/20.500.12123/10936
https://www.publish.csiro.au/cp/CP13222
https://doi.org/10.1071/CP13222
identifier_str_mv 1836-0947
1836-5795 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv CSIRO Publishing
publisher.none.fl_str_mv CSIRO Publishing
dc.source.none.fl_str_mv Crop and Pasture Science 65 (2) : 139-149 (2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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