Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress

Autores
Melchiorre, Mariana; Quero, Gastón E.; Parola, Rodrigo; Racca, Roberto Walter; Trippi, Victorio; Lascano, Hernan Ramiro
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genus Lotus comprises a heterogeneous group of annual and perennial species. Lotus japonicus (with MG20 and Gifu ecotypes) has been adopted as one of the model legumes in genetic and genomic studies. Other Lotus species, such us Lotus burttii and Lotus filicaulis, have also been used in genetic and genomic studies because of their capacity to produce fertile progenies in crosses with L. japonicus. In the present work, physiological responses to salt stress in four Lotus genotypes were evaluated on the basis of growth and associated parameters, such as photosynthesis, ions, relative water content, oxidative damage and antioxidant system responses, using two NaCl levels applied by acclimation for up to 28 and 60 d. Growth responses varied with plant developmental stage in the four Lotus genotypes. L. japonicus MG20 was found to be a salt-tolerant genotype, mainly when exposed to salt stress at the young plant stage. The capacity of Lj MG20 to sustain growth under salt stress was correlated with enhancement of Superoxide dismutase and Glutathione reductase activities, as well as with increases in total and reduced glutathione content and lower Na+ accumulation in leaves. These results suggest that enhancement of antioxidant responses in Lj MG20 contributed to improve salt stress tolerance at early stages. On the other hand, after long-term high NaCl stress treatment, L. filicaulis exhibited lower biomass reduction, lower oxidative damage and Na+ accumulation in leaves than the control treatment; hence, this genotype was considered salt-tolerant. These apparently ambiguous results remark that salt tolerance, as a development-related process, was differentially expressed among the Lotus genotypes and depended on stress duration and plant phenological stage.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Quero, Gastón E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Quero, Gastón E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Parola, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Trippi, Victorio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trippi, Victorio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fuente
Plant Science 177 (6) : 618-628 (December 2009)
Materia
Ions
Salinity
Osmotic Stress
Lotus
Estrés Osmótico
Iones
Salinidad
Antioxidant System
Phenotyping
Estrés Salino
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10979
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10979
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stressMelchiorre, MarianaQuero, Gastón E.Parola, RodrigoRacca, Roberto WalterTrippi, VictorioLascano, Hernan RamiroIonsSalinityOsmotic StressLotusEstrés OsmóticoIonesSalinidadAntioxidant SystemPhenotypingEstrés SalinoThe genus Lotus comprises a heterogeneous group of annual and perennial species. Lotus japonicus (with MG20 and Gifu ecotypes) has been adopted as one of the model legumes in genetic and genomic studies. Other Lotus species, such us Lotus burttii and Lotus filicaulis, have also been used in genetic and genomic studies because of their capacity to produce fertile progenies in crosses with L. japonicus. In the present work, physiological responses to salt stress in four Lotus genotypes were evaluated on the basis of growth and associated parameters, such as photosynthesis, ions, relative water content, oxidative damage and antioxidant system responses, using two NaCl levels applied by acclimation for up to 28 and 60 d. Growth responses varied with plant developmental stage in the four Lotus genotypes. L. japonicus MG20 was found to be a salt-tolerant genotype, mainly when exposed to salt stress at the young plant stage. The capacity of Lj MG20 to sustain growth under salt stress was correlated with enhancement of Superoxide dismutase and Glutathione reductase activities, as well as with increases in total and reduced glutathione content and lower Na+ accumulation in leaves. These results suggest that enhancement of antioxidant responses in Lj MG20 contributed to improve salt stress tolerance at early stages. On the other hand, after long-term high NaCl stress treatment, L. filicaulis exhibited lower biomass reduction, lower oxidative damage and Na+ accumulation in leaves than the control treatment; hence, this genotype was considered salt-tolerant. These apparently ambiguous results remark that salt tolerance, as a development-related process, was differentially expressed among the Lotus genotypes and depended on stress duration and plant phenological stage.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Quero, Gastón E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Quero, Gastón E. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Parola, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Trippi, Victorio. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trippi, Victorio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; ArgentinaElsevier2021-12-23T11:08:05Z2021-12-23T11:08:05Z2009-09-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10979https://www.sciencedirect.com/science/article/pii/S01689452090025810168-9452https://doi.org/10.1016/j.plantsci.2009.09.010Plant Science 177 (6) : 618-628 (December 2009)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:13Zoai:localhost:20.500.12123/10979instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:14.288INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress
title Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress
spellingShingle Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress
Melchiorre, Mariana
Ions
Salinity
Osmotic Stress
Lotus
Estrés Osmótico
Iones
Salinidad
Antioxidant System
Phenotyping
Estrés Salino
title_short Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress
title_full Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress
title_fullStr Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress
title_full_unstemmed Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress
title_sort Physiological characterization of four model Lotus diploid genotypes: L. japonicus (MG20 and Gifu), L. filicaulis, and L. burttii under salt stress
dc.creator.none.fl_str_mv Melchiorre, Mariana
Quero, Gastón E.
Parola, Rodrigo
Racca, Roberto Walter
Trippi, Victorio
Lascano, Hernan Ramiro
author Melchiorre, Mariana
author_facet Melchiorre, Mariana
Quero, Gastón E.
Parola, Rodrigo
Racca, Roberto Walter
Trippi, Victorio
Lascano, Hernan Ramiro
author_role author
author2 Quero, Gastón E.
Parola, Rodrigo
Racca, Roberto Walter
Trippi, Victorio
Lascano, Hernan Ramiro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ions
Salinity
Osmotic Stress
Lotus
Estrés Osmótico
Iones
Salinidad
Antioxidant System
Phenotyping
Estrés Salino
topic Ions
Salinity
Osmotic Stress
Lotus
Estrés Osmótico
Iones
Salinidad
Antioxidant System
Phenotyping
Estrés Salino
dc.description.none.fl_txt_mv The genus Lotus comprises a heterogeneous group of annual and perennial species. Lotus japonicus (with MG20 and Gifu ecotypes) has been adopted as one of the model legumes in genetic and genomic studies. Other Lotus species, such us Lotus burttii and Lotus filicaulis, have also been used in genetic and genomic studies because of their capacity to produce fertile progenies in crosses with L. japonicus. In the present work, physiological responses to salt stress in four Lotus genotypes were evaluated on the basis of growth and associated parameters, such as photosynthesis, ions, relative water content, oxidative damage and antioxidant system responses, using two NaCl levels applied by acclimation for up to 28 and 60 d. Growth responses varied with plant developmental stage in the four Lotus genotypes. L. japonicus MG20 was found to be a salt-tolerant genotype, mainly when exposed to salt stress at the young plant stage. The capacity of Lj MG20 to sustain growth under salt stress was correlated with enhancement of Superoxide dismutase and Glutathione reductase activities, as well as with increases in total and reduced glutathione content and lower Na+ accumulation in leaves. These results suggest that enhancement of antioxidant responses in Lj MG20 contributed to improve salt stress tolerance at early stages. On the other hand, after long-term high NaCl stress treatment, L. filicaulis exhibited lower biomass reduction, lower oxidative damage and Na+ accumulation in leaves than the control treatment; hence, this genotype was considered salt-tolerant. These apparently ambiguous results remark that salt tolerance, as a development-related process, was differentially expressed among the Lotus genotypes and depended on stress duration and plant phenological stage.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Quero, Gastón E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Quero, Gastón E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Parola, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Trippi, Victorio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trippi, Victorio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
description The genus Lotus comprises a heterogeneous group of annual and perennial species. Lotus japonicus (with MG20 and Gifu ecotypes) has been adopted as one of the model legumes in genetic and genomic studies. Other Lotus species, such us Lotus burttii and Lotus filicaulis, have also been used in genetic and genomic studies because of their capacity to produce fertile progenies in crosses with L. japonicus. In the present work, physiological responses to salt stress in four Lotus genotypes were evaluated on the basis of growth and associated parameters, such as photosynthesis, ions, relative water content, oxidative damage and antioxidant system responses, using two NaCl levels applied by acclimation for up to 28 and 60 d. Growth responses varied with plant developmental stage in the four Lotus genotypes. L. japonicus MG20 was found to be a salt-tolerant genotype, mainly when exposed to salt stress at the young plant stage. The capacity of Lj MG20 to sustain growth under salt stress was correlated with enhancement of Superoxide dismutase and Glutathione reductase activities, as well as with increases in total and reduced glutathione content and lower Na+ accumulation in leaves. These results suggest that enhancement of antioxidant responses in Lj MG20 contributed to improve salt stress tolerance at early stages. On the other hand, after long-term high NaCl stress treatment, L. filicaulis exhibited lower biomass reduction, lower oxidative damage and Na+ accumulation in leaves than the control treatment; hence, this genotype was considered salt-tolerant. These apparently ambiguous results remark that salt tolerance, as a development-related process, was differentially expressed among the Lotus genotypes and depended on stress duration and plant phenological stage.
publishDate 2009
dc.date.none.fl_str_mv 2009-09-25
2021-12-23T11:08:05Z
2021-12-23T11:08:05Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10979
https://www.sciencedirect.com/science/article/pii/S0168945209002581
0168-9452
https://doi.org/10.1016/j.plantsci.2009.09.010
url http://hdl.handle.net/20.500.12123/10979
https://www.sciencedirect.com/science/article/pii/S0168945209002581
https://doi.org/10.1016/j.plantsci.2009.09.010
identifier_str_mv 0168-9452
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Plant Science 177 (6) : 618-628 (December 2009)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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