Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution

Autores
Dallard, Bibiana Elisabet; Pujato, Silvina A.; Baravalle, Celina; Pereyra, Elizabet Amanda Lorena; Rey, Florencia; Renna, Maria Sol; Calvinho, Luis Fernando
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng extract (GS) on insulin-like growth factors (IGF) in bovine mammary gland during early involution. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of ginseng extract solution (3 mg/mL), six quarters were treated with 10 mL of placebo (vehicle alone) and six quarters were maintained as uninoculated controls. Milking was interrupted after infusion. Concentrations of IGF1 in mammary secretions were higher in GS-treated quarters than in placebo and uninoculated control quarters at 24, 48 and 72 h post-treatment (p < 0.05). Treatment with GS did not affect mammary secretion of IGF2 (p = 0.942). At 7 d of post-lactational involution, a decrease of immunostained area and mRNA expression for IGF1 was observed in mammary tissue of GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p < 0.05). The IGF2 immunostained area and mRNA expression for this growth factor were not affected by GS treatment (p = 0.216 and p = 0.785, respectively). An increase in protein levels and mRNA expression in mammary tissue of IGFBP3, IGFBP4 and IGFBP5 was observed in GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p < 0.05). These results provide evidence that intramammary inoculation of GS extract at cessation of milking may promote early mammary involution through the inhibition of IGF1 local production and bioavailability.
EEA Rafaela
Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pujato, Silvina A.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Baravalle, Celina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pereyra, Elizabet Amanda Lorena. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rey, Florencia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Renna, Maria Sol. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fuente
Research in Veterinary Science 94 (3) : 462-470 (June 2013)
Materia
Panax pseudoginseng
Extractos
Glándulas Mamarias
Ganado Bovino
Ordeño
Factor de Crecimiento Igf
Extracts
Mammary Glands
Cattle
Milking
Insulin-like Growth Factor
Panax ginseng
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3074
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3074
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involutionDallard, Bibiana ElisabetPujato, Silvina A.Baravalle, CelinaPereyra, Elizabet Amanda LorenaRey, FlorenciaRenna, Maria SolCalvinho, Luis FernandoPanax pseudoginsengExtractosGlándulas MamariasGanado BovinoOrdeñoFactor de Crecimiento IgfExtractsMammary GlandsCattleMilkingInsulin-like Growth FactorPanax ginsengThe objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng extract (GS) on insulin-like growth factors (IGF) in bovine mammary gland during early involution. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of ginseng extract solution (3 mg/mL), six quarters were treated with 10 mL of placebo (vehicle alone) and six quarters were maintained as uninoculated controls. Milking was interrupted after infusion. Concentrations of IGF1 in mammary secretions were higher in GS-treated quarters than in placebo and uninoculated control quarters at 24, 48 and 72 h post-treatment (p < 0.05). Treatment with GS did not affect mammary secretion of IGF2 (p = 0.942). At 7 d of post-lactational involution, a decrease of immunostained area and mRNA expression for IGF1 was observed in mammary tissue of GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p < 0.05). The IGF2 immunostained area and mRNA expression for this growth factor were not affected by GS treatment (p = 0.216 and p = 0.785, respectively). An increase in protein levels and mRNA expression in mammary tissue of IGFBP3, IGFBP4 and IGFBP5 was observed in GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p < 0.05). These results provide evidence that intramammary inoculation of GS extract at cessation of milking may promote early mammary involution through the inhibition of IGF1 local production and bioavailability.EEA RafaelaFil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pujato, Silvina A.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Baravalle, Celina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pereyra, Elizabet Amanda Lorena. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rey, Florencia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Renna, Maria Sol. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina2018-08-15T12:10:58Z2018-08-15T12:10:58Z2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0034528813000118http://hdl.handle.net/20.500.12123/30740034-5288https://doi.org/10.1016/j.rvsc.2013.01.009Research in Veterinary Science 94 (3) : 462-470 (June 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-18T10:07:16Zoai:localhost:20.500.12123/3074instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:07:16.718INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution
title Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution
spellingShingle Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution
Dallard, Bibiana Elisabet
Panax pseudoginseng
Extractos
Glándulas Mamarias
Ganado Bovino
Ordeño
Factor de Crecimiento Igf
Extracts
Mammary Glands
Cattle
Milking
Insulin-like Growth Factor
Panax ginseng
title_short Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution
title_full Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution
title_fullStr Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution
title_full_unstemmed Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution
title_sort Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution
dc.creator.none.fl_str_mv Dallard, Bibiana Elisabet
Pujato, Silvina A.
Baravalle, Celina
Pereyra, Elizabet Amanda Lorena
Rey, Florencia
Renna, Maria Sol
Calvinho, Luis Fernando
author Dallard, Bibiana Elisabet
author_facet Dallard, Bibiana Elisabet
Pujato, Silvina A.
Baravalle, Celina
Pereyra, Elizabet Amanda Lorena
Rey, Florencia
Renna, Maria Sol
Calvinho, Luis Fernando
author_role author
author2 Pujato, Silvina A.
Baravalle, Celina
Pereyra, Elizabet Amanda Lorena
Rey, Florencia
Renna, Maria Sol
Calvinho, Luis Fernando
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Panax pseudoginseng
Extractos
Glándulas Mamarias
Ganado Bovino
Ordeño
Factor de Crecimiento Igf
Extracts
Mammary Glands
Cattle
Milking
Insulin-like Growth Factor
Panax ginseng
topic Panax pseudoginseng
Extractos
Glándulas Mamarias
Ganado Bovino
Ordeño
Factor de Crecimiento Igf
Extracts
Mammary Glands
Cattle
Milking
Insulin-like Growth Factor
Panax ginseng
dc.description.none.fl_txt_mv The objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng extract (GS) on insulin-like growth factors (IGF) in bovine mammary gland during early involution. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of ginseng extract solution (3 mg/mL), six quarters were treated with 10 mL of placebo (vehicle alone) and six quarters were maintained as uninoculated controls. Milking was interrupted after infusion. Concentrations of IGF1 in mammary secretions were higher in GS-treated quarters than in placebo and uninoculated control quarters at 24, 48 and 72 h post-treatment (p < 0.05). Treatment with GS did not affect mammary secretion of IGF2 (p = 0.942). At 7 d of post-lactational involution, a decrease of immunostained area and mRNA expression for IGF1 was observed in mammary tissue of GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p < 0.05). The IGF2 immunostained area and mRNA expression for this growth factor were not affected by GS treatment (p = 0.216 and p = 0.785, respectively). An increase in protein levels and mRNA expression in mammary tissue of IGFBP3, IGFBP4 and IGFBP5 was observed in GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p < 0.05). These results provide evidence that intramammary inoculation of GS extract at cessation of milking may promote early mammary involution through the inhibition of IGF1 local production and bioavailability.
EEA Rafaela
Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pujato, Silvina A.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Baravalle, Celina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pereyra, Elizabet Amanda Lorena. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rey, Florencia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Renna, Maria Sol. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
description The objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng extract (GS) on insulin-like growth factors (IGF) in bovine mammary gland during early involution. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of ginseng extract solution (3 mg/mL), six quarters were treated with 10 mL of placebo (vehicle alone) and six quarters were maintained as uninoculated controls. Milking was interrupted after infusion. Concentrations of IGF1 in mammary secretions were higher in GS-treated quarters than in placebo and uninoculated control quarters at 24, 48 and 72 h post-treatment (p < 0.05). Treatment with GS did not affect mammary secretion of IGF2 (p = 0.942). At 7 d of post-lactational involution, a decrease of immunostained area and mRNA expression for IGF1 was observed in mammary tissue of GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p < 0.05). The IGF2 immunostained area and mRNA expression for this growth factor were not affected by GS treatment (p = 0.216 and p = 0.785, respectively). An increase in protein levels and mRNA expression in mammary tissue of IGFBP3, IGFBP4 and IGFBP5 was observed in GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p < 0.05). These results provide evidence that intramammary inoculation of GS extract at cessation of milking may promote early mammary involution through the inhibition of IGF1 local production and bioavailability.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
2018-08-15T12:10:58Z
2018-08-15T12:10:58Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0034528813000118
http://hdl.handle.net/20.500.12123/3074
0034-5288
https://doi.org/10.1016/j.rvsc.2013.01.009
url https://www.sciencedirect.com/science/article/pii/S0034528813000118
http://hdl.handle.net/20.500.12123/3074
https://doi.org/10.1016/j.rvsc.2013.01.009
identifier_str_mv 0034-5288
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Research in Veterinary Science 94 (3) : 462-470 (June 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.001348

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