Use of molecular tools for the diagnosis of rangeliosis by Rangelia vitalii in Argentina: A case report

Autores
Borras, Pablo; Salvador, F.; Rinaldi, V.; Armitano, Rita Inés; Farber, Marisa Diana; Sanchez, R.; Mori, L.; Guillemi, Eliana Carolina
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Vector-borne pathogens are responsible for serious emerging diseases and Rangelia vitalii, the etiologic agent of canine rangeliosis, is one of the most pathogenic tick-borne pathogens for dogs in South America. This protozoan is transmitted by the Amblyomma aureolatum tick bite and the clinical features associated to the disease are fever, hemolytic anemia, jaundice, hepatosplenomegaly and bleeding from natural orifices, mainly from the ear egde. The reports of canine rangeliosis in Argentina are scarce. In the present study we report the detection of Rangelia vitalii in a naturally infected dog from Gualeguay, Entre Ríos, Argentina with history of tick infestation and clinical signs compatible with rangeliosis. An initial blood sample was positive to piroplasmids by blood smear examination and the molecular amplification of a fragment of the 18SrRNA gene. Sequencing of the fragment confirmed the pathogen identity. After treatment with imidocarb dipropionate, the clinical signs remitted and the blood smear tested negative.
Instituto de Biotecnología
Fil: Borras, Pablo. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán" (ANLIS). Centro Nacional de Diagnóstico e Investigación en Endemo Epidemias (CENDIE); Argentina
Fil: Salvador, F. Laboratorio MF Salvador; Argentina
Fil: Rinaldi, V. Laboratorio MF Salvador; Argentina
Fil: Armitano, Rita Inés. Instituto Nacional de Enfermedades Infecciosas. Servicio Bacteriología Especial; Argentina
Fil: Armitano, Rita Inés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina.
Fil: Farber, Marisa Diana. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán" (ANLIS). INEI. Servicio Bacteriología Especial; Argentina
Fil: Sanchez, R. ROSLAB Diagnostico Veterinario; Argentina
Fil: Mori, L. Laboratorio MF Salvador; Argentina
Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Guillemi, Eliana Carolina. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán" (ANLIS). INEI. Servicio Bacteriología Especial; Argentina
Fuente
Veterinary Parasitology: Regional Studies and Reports 21 : 100426 (July 2020)
Materia
Enfermedades Transmitidas Vectores
Enfermedades de los Animales
Diagnóstico
Piroplásmea
Perro
Vector-borne Diseases
Animal Diseases
Diagnosis
Piroplasmea
Dogs
Argentina
Rangelia vitalii
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11120
Acceder
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oai_identifier_str oai:localhost:20.500.12123/11120
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Use of molecular tools for the diagnosis of rangeliosis by Rangelia vitalii in Argentina: A case reportBorras, PabloSalvador, F.Rinaldi, V.Armitano, Rita InésFarber, Marisa DianaSanchez, R.Mori, L.Guillemi, Eliana CarolinaEnfermedades Transmitidas VectoresEnfermedades de los AnimalesDiagnósticoPiroplásmeaPerroVector-borne DiseasesAnimal DiseasesDiagnosisPiroplasmeaDogsArgentinaRangelia vitaliiVector-borne pathogens are responsible for serious emerging diseases and Rangelia vitalii, the etiologic agent of canine rangeliosis, is one of the most pathogenic tick-borne pathogens for dogs in South America. This protozoan is transmitted by the Amblyomma aureolatum tick bite and the clinical features associated to the disease are fever, hemolytic anemia, jaundice, hepatosplenomegaly and bleeding from natural orifices, mainly from the ear egde. The reports of canine rangeliosis in Argentina are scarce. In the present study we report the detection of Rangelia vitalii in a naturally infected dog from Gualeguay, Entre Ríos, Argentina with history of tick infestation and clinical signs compatible with rangeliosis. An initial blood sample was positive to piroplasmids by blood smear examination and the molecular amplification of a fragment of the 18SrRNA gene. Sequencing of the fragment confirmed the pathogen identity. After treatment with imidocarb dipropionate, the clinical signs remitted and the blood smear tested negative.Instituto de BiotecnologíaFil: Borras, Pablo. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán" (ANLIS). Centro Nacional de Diagnóstico e Investigación en Endemo Epidemias (CENDIE); ArgentinaFil: Salvador, F. Laboratorio MF Salvador; ArgentinaFil: Rinaldi, V. Laboratorio MF Salvador; ArgentinaFil: Armitano, Rita Inés. Instituto Nacional de Enfermedades Infecciosas. Servicio Bacteriología Especial; ArgentinaFil: Armitano, Rita Inés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina.Fil: Farber, Marisa Diana. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán" (ANLIS). INEI. Servicio Bacteriología Especial; ArgentinaFil: Sanchez, R. ROSLAB Diagnostico Veterinario; ArgentinaFil: Mori, L. Laboratorio MF Salvador; ArgentinaFil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Guillemi, Eliana Carolina. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán" (ANLIS). INEI. Servicio Bacteriología Especial; ArgentinaElsevier2022-01-14T10:51:09Z2022-01-14T10:51:09Z2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11120https://www.sciencedirect.com/science/article/abs/pii/S24059390203020702405-9390https://doi.org/10.1016/j.vprsr.2020.100426Veterinary Parasitology: Regional Studies and Reports 21 : 100426 (July 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:17:50Zoai:localhost:20.500.12123/11120instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:51.322INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Use of molecular tools for the diagnosis of rangeliosis by Rangelia vitalii in Argentina: A case report
title Use of molecular tools for the diagnosis of rangeliosis by Rangelia vitalii in Argentina: A case report
spellingShingle Use of molecular tools for the diagnosis of rangeliosis by Rangelia vitalii in Argentina: A case report
Borras, Pablo
Enfermedades Transmitidas Vectores
Enfermedades de los Animales
Diagnóstico
Piroplásmea
Perro
Vector-borne Diseases
Animal Diseases
Diagnosis
Piroplasmea
Dogs
Argentina
Rangelia vitalii
title_short Use of molecular tools for the diagnosis of rangeliosis by Rangelia vitalii in Argentina: A case report
title_full Use of molecular tools for the diagnosis of rangeliosis by Rangelia vitalii in Argentina: A case report
title_fullStr Use of molecular tools for the diagnosis of rangeliosis by Rangelia vitalii in Argentina: A case report
title_full_unstemmed Use of molecular tools for the diagnosis of rangeliosis by Rangelia vitalii in Argentina: A case report
title_sort Use of molecular tools for the diagnosis of rangeliosis by Rangelia vitalii in Argentina: A case report
dc.creator.none.fl_str_mv Borras, Pablo
Salvador, F.
Rinaldi, V.
Armitano, Rita Inés
Farber, Marisa Diana
Sanchez, R.
Mori, L.
Guillemi, Eliana Carolina
author Borras, Pablo
author_facet Borras, Pablo
Salvador, F.
Rinaldi, V.
Armitano, Rita Inés
Farber, Marisa Diana
Sanchez, R.
Mori, L.
Guillemi, Eliana Carolina
author_role author
author2 Salvador, F.
Rinaldi, V.
Armitano, Rita Inés
Farber, Marisa Diana
Sanchez, R.
Mori, L.
Guillemi, Eliana Carolina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades Transmitidas Vectores
Enfermedades de los Animales
Diagnóstico
Piroplásmea
Perro
Vector-borne Diseases
Animal Diseases
Diagnosis
Piroplasmea
Dogs
Argentina
Rangelia vitalii
topic Enfermedades Transmitidas Vectores
Enfermedades de los Animales
Diagnóstico
Piroplásmea
Perro
Vector-borne Diseases
Animal Diseases
Diagnosis
Piroplasmea
Dogs
Argentina
Rangelia vitalii
dc.description.none.fl_txt_mv Vector-borne pathogens are responsible for serious emerging diseases and Rangelia vitalii, the etiologic agent of canine rangeliosis, is one of the most pathogenic tick-borne pathogens for dogs in South America. This protozoan is transmitted by the Amblyomma aureolatum tick bite and the clinical features associated to the disease are fever, hemolytic anemia, jaundice, hepatosplenomegaly and bleeding from natural orifices, mainly from the ear egde. The reports of canine rangeliosis in Argentina are scarce. In the present study we report the detection of Rangelia vitalii in a naturally infected dog from Gualeguay, Entre Ríos, Argentina with history of tick infestation and clinical signs compatible with rangeliosis. An initial blood sample was positive to piroplasmids by blood smear examination and the molecular amplification of a fragment of the 18SrRNA gene. Sequencing of the fragment confirmed the pathogen identity. After treatment with imidocarb dipropionate, the clinical signs remitted and the blood smear tested negative.
Instituto de Biotecnología
Fil: Borras, Pablo. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán" (ANLIS). Centro Nacional de Diagnóstico e Investigación en Endemo Epidemias (CENDIE); Argentina
Fil: Salvador, F. Laboratorio MF Salvador; Argentina
Fil: Rinaldi, V. Laboratorio MF Salvador; Argentina
Fil: Armitano, Rita Inés. Instituto Nacional de Enfermedades Infecciosas. Servicio Bacteriología Especial; Argentina
Fil: Armitano, Rita Inés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina.
Fil: Farber, Marisa Diana. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán" (ANLIS). INEI. Servicio Bacteriología Especial; Argentina
Fil: Sanchez, R. ROSLAB Diagnostico Veterinario; Argentina
Fil: Mori, L. Laboratorio MF Salvador; Argentina
Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Guillemi, Eliana Carolina. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán" (ANLIS). INEI. Servicio Bacteriología Especial; Argentina
description Vector-borne pathogens are responsible for serious emerging diseases and Rangelia vitalii, the etiologic agent of canine rangeliosis, is one of the most pathogenic tick-borne pathogens for dogs in South America. This protozoan is transmitted by the Amblyomma aureolatum tick bite and the clinical features associated to the disease are fever, hemolytic anemia, jaundice, hepatosplenomegaly and bleeding from natural orifices, mainly from the ear egde. The reports of canine rangeliosis in Argentina are scarce. In the present study we report the detection of Rangelia vitalii in a naturally infected dog from Gualeguay, Entre Ríos, Argentina with history of tick infestation and clinical signs compatible with rangeliosis. An initial blood sample was positive to piroplasmids by blood smear examination and the molecular amplification of a fragment of the 18SrRNA gene. Sequencing of the fragment confirmed the pathogen identity. After treatment with imidocarb dipropionate, the clinical signs remitted and the blood smear tested negative.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
2022-01-14T10:51:09Z
2022-01-14T10:51:09Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11120
https://www.sciencedirect.com/science/article/abs/pii/S2405939020302070
2405-9390
https://doi.org/10.1016/j.vprsr.2020.100426
url http://hdl.handle.net/20.500.12123/11120
https://www.sciencedirect.com/science/article/abs/pii/S2405939020302070
https://doi.org/10.1016/j.vprsr.2020.100426
identifier_str_mv 2405-9390
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Parasitology: Regional Studies and Reports 21 : 100426 (July 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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