First report of Rangelia vitalii infection (canine rangeliosis) in Argentina

Autores
Eiras, Diego Fernando; Craviotto, Maria Belen; Baneth, Gad; Moré, Gastón Andrés
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A 12-year old mixed breed neutered bitch from Misiones, Argentina, was presented with a history of fever and epistaxis. Blood, bone marrow, and lymph node samples were collected for hematology and cytology. Mild regenerative anemia was recorded and large, round, poorly stained piroplasms (> 2.5 μm) were found within erythrocytes in blood and lymph node smears. Nested PCR-RFLP on blood and bone marrow samples was positive for piroplasm DNA. The 18S rRNA gene of piroplasms was targeted. A restriction pattern of a previously unreported piroplasm was observed. The PCR product was sequenced, and the sequence obtained had 99% identity with the Rangelia vitalii sequences from Brazil when compared by BLAST analysis. Further characterization of the detected piroplasm consisted of nearly full-length sequencing (1668 bp) of the 18S rRNA gene of this organism. Those sequences were deposited in GenBank. A phylogenetic analysis indicated that they clustered together with R. vitalii from Brazil but separately from large Babesia species of dogs such as Babesia canis, and from species of Theileria of dogs as well. This is the first report of R. vitalii infection in Argentina, and the first case of canine rangeliosis diagnosed outside Brazil.
Fil: Eiras, Diego Fernando. Laboratorio Diagnóstico en Animales Pequeños. Banfield; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Craviotto, Maria Belen. Laboratorio Diagnóstico en Animales Pequeños. Banfield; Argentina
Fil: Baneth, Gad. The Hebrew University Of Jerusalem; Israel
Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Materia
Canine
Piroplasm
Rangelia Vitalii
Rangeliosis
Nambiuvú
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32573
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/32573
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First report of Rangelia vitalii infection (canine rangeliosis) in ArgentinaEiras, Diego FernandoCraviotto, Maria BelenBaneth, GadMoré, Gastón AndrésCaninePiroplasmRangelia VitaliiRangeliosisNambiuvúArgentinaA 12-year old mixed breed neutered bitch from Misiones, Argentina, was presented with a history of fever and epistaxis. Blood, bone marrow, and lymph node samples were collected for hematology and cytology. Mild regenerative anemia was recorded and large, round, poorly stained piroplasms (> 2.5 μm) were found within erythrocytes in blood and lymph node smears. Nested PCR-RFLP on blood and bone marrow samples was positive for piroplasm DNA. The 18S rRNA gene of piroplasms was targeted. A restriction pattern of a previously unreported piroplasm was observed. The PCR product was sequenced, and the sequence obtained had 99% identity with the Rangelia vitalii sequences from Brazil when compared by BLAST analysis. Further characterization of the detected piroplasm consisted of nearly full-length sequencing (1668 bp) of the 18S rRNA gene of this organism. Those sequences were deposited in GenBank. A phylogenetic analysis indicated that they clustered together with R. vitalii from Brazil but separately from large Babesia species of dogs such as Babesia canis, and from species of Theileria of dogs as well. This is the first report of R. vitalii infection in Argentina, and the first case of canine rangeliosis diagnosed outside Brazil.Fil: Eiras, Diego Fernando. Laboratorio Diagnóstico en Animales Pequeños. Banfield; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Craviotto, Maria Belen. Laboratorio Diagnóstico en Animales Pequeños. Banfield; ArgentinaFil: Baneth, Gad. The Hebrew University Of Jerusalem; IsraelFil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaElsevier Ireland2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32573Baneth, Gad; Moré, Gastón Andrés; Craviotto, Maria Belen; Eiras, Diego Fernando; First report of Rangelia vitalii infection (canine rangeliosis) in Argentina; Elsevier Ireland; Parasitology International; 63; 5; 6-2014; 729-7341383-5769CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.parint.2014.06.003info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1383576914000774info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:57Zoai:ri.conicet.gov.ar:11336/32573instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:58.263CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First report of Rangelia vitalii infection (canine rangeliosis) in Argentina
title First report of Rangelia vitalii infection (canine rangeliosis) in Argentina
spellingShingle First report of Rangelia vitalii infection (canine rangeliosis) in Argentina
Eiras, Diego Fernando
Canine
Piroplasm
Rangelia Vitalii
Rangeliosis
Nambiuvú
Argentina
title_short First report of Rangelia vitalii infection (canine rangeliosis) in Argentina
title_full First report of Rangelia vitalii infection (canine rangeliosis) in Argentina
title_fullStr First report of Rangelia vitalii infection (canine rangeliosis) in Argentina
title_full_unstemmed First report of Rangelia vitalii infection (canine rangeliosis) in Argentina
title_sort First report of Rangelia vitalii infection (canine rangeliosis) in Argentina
dc.creator.none.fl_str_mv Eiras, Diego Fernando
Craviotto, Maria Belen
Baneth, Gad
Moré, Gastón Andrés
author Eiras, Diego Fernando
author_facet Eiras, Diego Fernando
Craviotto, Maria Belen
Baneth, Gad
Moré, Gastón Andrés
author_role author
author2 Craviotto, Maria Belen
Baneth, Gad
Moré, Gastón Andrés
author2_role author
author
author
dc.subject.none.fl_str_mv Canine
Piroplasm
Rangelia Vitalii
Rangeliosis
Nambiuvú
Argentina
topic Canine
Piroplasm
Rangelia Vitalii
Rangeliosis
Nambiuvú
Argentina
dc.description.none.fl_txt_mv A 12-year old mixed breed neutered bitch from Misiones, Argentina, was presented with a history of fever and epistaxis. Blood, bone marrow, and lymph node samples were collected for hematology and cytology. Mild regenerative anemia was recorded and large, round, poorly stained piroplasms (> 2.5 μm) were found within erythrocytes in blood and lymph node smears. Nested PCR-RFLP on blood and bone marrow samples was positive for piroplasm DNA. The 18S rRNA gene of piroplasms was targeted. A restriction pattern of a previously unreported piroplasm was observed. The PCR product was sequenced, and the sequence obtained had 99% identity with the Rangelia vitalii sequences from Brazil when compared by BLAST analysis. Further characterization of the detected piroplasm consisted of nearly full-length sequencing (1668 bp) of the 18S rRNA gene of this organism. Those sequences were deposited in GenBank. A phylogenetic analysis indicated that they clustered together with R. vitalii from Brazil but separately from large Babesia species of dogs such as Babesia canis, and from species of Theileria of dogs as well. This is the first report of R. vitalii infection in Argentina, and the first case of canine rangeliosis diagnosed outside Brazil.
Fil: Eiras, Diego Fernando. Laboratorio Diagnóstico en Animales Pequeños. Banfield; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
Fil: Craviotto, Maria Belen. Laboratorio Diagnóstico en Animales Pequeños. Banfield; Argentina
Fil: Baneth, Gad. The Hebrew University Of Jerusalem; Israel
Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina
description A 12-year old mixed breed neutered bitch from Misiones, Argentina, was presented with a history of fever and epistaxis. Blood, bone marrow, and lymph node samples were collected for hematology and cytology. Mild regenerative anemia was recorded and large, round, poorly stained piroplasms (> 2.5 μm) were found within erythrocytes in blood and lymph node smears. Nested PCR-RFLP on blood and bone marrow samples was positive for piroplasm DNA. The 18S rRNA gene of piroplasms was targeted. A restriction pattern of a previously unreported piroplasm was observed. The PCR product was sequenced, and the sequence obtained had 99% identity with the Rangelia vitalii sequences from Brazil when compared by BLAST analysis. Further characterization of the detected piroplasm consisted of nearly full-length sequencing (1668 bp) of the 18S rRNA gene of this organism. Those sequences were deposited in GenBank. A phylogenetic analysis indicated that they clustered together with R. vitalii from Brazil but separately from large Babesia species of dogs such as Babesia canis, and from species of Theileria of dogs as well. This is the first report of R. vitalii infection in Argentina, and the first case of canine rangeliosis diagnosed outside Brazil.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32573
Baneth, Gad; Moré, Gastón Andrés; Craviotto, Maria Belen; Eiras, Diego Fernando; First report of Rangelia vitalii infection (canine rangeliosis) in Argentina; Elsevier Ireland; Parasitology International; 63; 5; 6-2014; 729-734
1383-5769
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32573
identifier_str_mv Baneth, Gad; Moré, Gastón Andrés; Craviotto, Maria Belen; Eiras, Diego Fernando; First report of Rangelia vitalii infection (canine rangeliosis) in Argentina; Elsevier Ireland; Parasitology International; 63; 5; 6-2014; 729-734
1383-5769
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.parint.2014.06.003
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1383576914000774
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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