Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress

Autores
Forrellad, Marina A.; Vázquez, Cristina L.; Blanco, Federico C.; Klepp, Laura Inés; García, Elizabeth A.; Rocha, Rosana V.; Villafañe, Luciana; Bigi, María Mercedes
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Forrellad, Marina A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Vázquez, Cristina L. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Blanco, Federico C. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Klepp, Laura Inés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: García, Elizabeth A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Rocha, Rosana V. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Villafañe, Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.
In this study, we characterized the role of Rv2617c in the virulence of Mycobacterium tuberculosis. Rv2617c is a protein of unknown function unique to M. tuberculosis complex (MTC) and Mycobacterium leprae. In vitro, this protein interacts with the virulence factor P36 (also named Erp) and KdpF, a protein linked to nitrosative stress. Here, we showed that knockout of the Rv2617c gene in M. tuberculosis CDC1551 reduced the replication of the pathogen in a mouse model of infection and favored the trafficking of mycobacteria to phagolysosomes. We also demonstrated that Rv2617c and P36 are required for resistance to in vitro hydrogen peroxide treatment in M. tuberculosis and Mycobacterium bovis, respectively. These findings indicate Rv2617c and P36 act in concert to prevent bacterial damage upon oxidative stress.
grafs., fot.
Fuente
Virulence
Vol.10, no.1
1026–1033
https://taylorandfrancis.com
Materia
MYCOBACTERIUM TUBERCULOSIS
OXIDATIVE STRESS
VIRULENCE FACTOR
MYCOBACTERIUM TUBERCULOSIS
RV2617C
ERP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
acceso abierto
Repositorio
FAUBA Digital (UBA-FAUBA)
Institución
Universidad de Buenos Aires. Facultad de Agronomía
OAI Identificador
snrd:2019forrellad
Acceder
id FAUBA_88efb7cf3b28443ebab861e534a713c0
oai_identifier_str snrd:2019forrellad
network_acronym_str FAUBA
repository_id_str 2729
network_name_str FAUBA Digital (UBA-FAUBA)
spelling Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stressForrellad, Marina A.Vázquez, Cristina L.Blanco, Federico C.Klepp, Laura InésGarcía, Elizabeth A.Rocha, Rosana V.Villafañe, LucianaBigi, María MercedesMYCOBACTERIUM TUBERCULOSISOXIDATIVE STRESSVIRULENCE FACTORMYCOBACTERIUM TUBERCULOSISRV2617CERPFil: Forrellad, Marina A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.Fil: Vázquez, Cristina L. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.Fil: Blanco, Federico C. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.Fil: Klepp, Laura Inés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.Fil: García, Elizabeth A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.Fil: Rocha, Rosana V. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.Fil: Villafañe, Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.In this study, we characterized the role of Rv2617c in the virulence of Mycobacterium tuberculosis. Rv2617c is a protein of unknown function unique to M. tuberculosis complex (MTC) and Mycobacterium leprae. In vitro, this protein interacts with the virulence factor P36 (also named Erp) and KdpF, a protein linked to nitrosative stress. Here, we showed that knockout of the Rv2617c gene in M. tuberculosis CDC1551 reduced the replication of the pathogen in a mouse model of infection and favored the trafficking of mycobacteria to phagolysosomes. We also demonstrated that Rv2617c and P36 are required for resistance to in vitro hydrogen peroxide treatment in M. tuberculosis and Mycobacterium bovis, respectively. These findings indicate Rv2617c and P36 act in concert to prevent bacterial damage upon oxidative stress.grafs., fot.2019articleinfo:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfdoi:10.1080/21505594.2019.1693714issn:2150-5608http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2019forrelladVirulenceVol.10, no.11026–1033https://taylorandfrancis.comreponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaenginfo:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42025-09-29T13:41:21Zsnrd:2019forrelladinstacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292025-09-29 13:41:22.334FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse
dc.title.none.fl_str_mv Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress
title Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress
spellingShingle Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress
Forrellad, Marina A.
MYCOBACTERIUM TUBERCULOSIS
OXIDATIVE STRESS
VIRULENCE FACTOR
MYCOBACTERIUM TUBERCULOSIS
RV2617C
ERP
title_short Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress
title_full Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress
title_fullStr Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress
title_full_unstemmed Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress
title_sort Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress
dc.creator.none.fl_str_mv Forrellad, Marina A.
Vázquez, Cristina L.
Blanco, Federico C.
Klepp, Laura Inés
García, Elizabeth A.
Rocha, Rosana V.
Villafañe, Luciana
Bigi, María Mercedes
author Forrellad, Marina A.
author_facet Forrellad, Marina A.
Vázquez, Cristina L.
Blanco, Federico C.
Klepp, Laura Inés
García, Elizabeth A.
Rocha, Rosana V.
Villafañe, Luciana
Bigi, María Mercedes
author_role author
author2 Vázquez, Cristina L.
Blanco, Federico C.
Klepp, Laura Inés
García, Elizabeth A.
Rocha, Rosana V.
Villafañe, Luciana
Bigi, María Mercedes
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MYCOBACTERIUM TUBERCULOSIS
OXIDATIVE STRESS
VIRULENCE FACTOR
MYCOBACTERIUM TUBERCULOSIS
RV2617C
ERP
topic MYCOBACTERIUM TUBERCULOSIS
OXIDATIVE STRESS
VIRULENCE FACTOR
MYCOBACTERIUM TUBERCULOSIS
RV2617C
ERP
dc.description.none.fl_txt_mv Fil: Forrellad, Marina A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Vázquez, Cristina L. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Blanco, Federico C. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Klepp, Laura Inés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: García, Elizabeth A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Rocha, Rosana V. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Villafañe, Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.
In this study, we characterized the role of Rv2617c in the virulence of Mycobacterium tuberculosis. Rv2617c is a protein of unknown function unique to M. tuberculosis complex (MTC) and Mycobacterium leprae. In vitro, this protein interacts with the virulence factor P36 (also named Erp) and KdpF, a protein linked to nitrosative stress. Here, we showed that knockout of the Rv2617c gene in M. tuberculosis CDC1551 reduced the replication of the pathogen in a mouse model of infection and favored the trafficking of mycobacteria to phagolysosomes. We also demonstrated that Rv2617c and P36 are required for resistance to in vitro hydrogen peroxide treatment in M. tuberculosis and Mycobacterium bovis, respectively. These findings indicate Rv2617c and P36 act in concert to prevent bacterial damage upon oxidative stress.
grafs., fot.
description Fil: Forrellad, Marina A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv article
info:eu-repo/semantics/article
publishedVersion
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv doi:10.1080/21505594.2019.1693714
issn:2150-5608
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2019forrellad
identifier_str_mv doi:10.1080/21505594.2019.1693714
issn:2150-5608
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2019forrellad
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
eu_rights_str_mv openAccess
rights_invalid_str_mv openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Virulence
Vol.10, no.1
1026–1033
https://taylorandfrancis.com
reponame:FAUBA Digital (UBA-FAUBA)
instname:Universidad de Buenos Aires. Facultad de Agronomía
reponame_str FAUBA Digital (UBA-FAUBA)
collection FAUBA Digital (UBA-FAUBA)
instname_str Universidad de Buenos Aires. Facultad de Agronomía
repository.name.fl_str_mv FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía
repository.mail.fl_str_mv martino@agro.uba.ar;berasa@agro.uba.ar
_version_ 1844618856192016384
score 13.070432

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