Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern

Autores
Gambini, Andrés; Duque Rodríguez, Matteo; Rodríguez, María Belén; Briski, Olinda; Flores Bragulat, Ana P.; Demergassi, Natalia; Losinno, Luis; Salamone, Daniel Felipe
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Gambini, Andrés. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Producción Equina. Buenos Aires, Argentina. - CONICET. Buenos Aires, Argentina.
Fil: Duque Rodríguez, Matteo. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina.
Fil: Rodríguez, María Belén. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina.
Fil: Briski, Olinda. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina.
Fil: Flores Bragulat, Ana P. CONICET. Buenos Aires, Argentina. - Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Producción Equina. Río IV, Córdoba, Argentina.
Fil: Demergassi, Natalia. Fundación Temaikèn. Belén de Escobar, Buenos Aires, Argentina.
Fil: Losinno, Luis. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Producción Equina. Río IV, Córdoba, Argentina.
Fil: Salamone, Daniel Felipe. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina.
Several equids have gone extinct and many extant equids are currently considered vulnerable to critically endangered. This work aimed to evaluate whether domestic horse oocytes support preimplantation development of zebra embryos obtained by intracytoplasmic sperm injection (ICSI, zebroid) and cloning, and to study the Hippo signaling path way during the lineage specification of trophectoderm cells and inner cell mass cells. We first showed that zebra and horse sperm cells induce porcine oocyte activation and recruit maternal SMARCA4 during pronuclear formation. SMARCA4 recruitment showed to be independent of the genetic background of the injected sperm. No differences were found in blastocyst rate of ICSI hybrid (zebra spermatozoon into horse egg) embryos relative to the homospecific horse control group. Interestingly, zebra cloned blastocyst rate was significantly higher at day 8. Moreover, most ICSI and cloned horse and zebra blastocysts showed a similar expression pattern of SOX2 and nuclear YAP1 with the majority of the nuclei positive for YAP1, and most SOX2+ nuclei negative for YAP1. Here we demonstrated that horse oocytes support zebra preimplantation development of both, ICSI and cloned embryos, without compromising development to blastocyst, blastocyst cell number neither the expression of SOX2 and YAP1. Our results support the use of domestic horse oocytes as a model to study in vitro zebra embryos on behalf of preservation of valuable genetic.
grafs., fot.
Fuente
Plos One
Vol.15, no.9
e0238948
http://www.plos.org/
Materia
HORSE
GENETICS
OOCYTES
IMPLANTATION
ZEBRA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
acceso abierto
Repositorio
FAUBA Digital (UBA-FAUBA)
Institución
Universidad de Buenos Aires. Facultad de Agronomía
OAI Identificador
snrd:2020gambini
Acceder
id FAUBA_20d83d7989ec61e70e2e6b1a669d1325
oai_identifier_str snrd:2020gambini
network_acronym_str FAUBA
repository_id_str 2729
network_name_str FAUBA Digital (UBA-FAUBA)
spelling Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression patternGambini, AndrésDuque Rodríguez, MatteoRodríguez, María BelénBriski, OlindaFlores Bragulat, Ana P.Demergassi, NataliaLosinno, LuisSalamone, Daniel FelipeHORSEGENETICSOOCYTESIMPLANTATIONZEBRAFil: Gambini, Andrés. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Producción Equina. Buenos Aires, Argentina. - CONICET. Buenos Aires, Argentina.Fil: Duque Rodríguez, Matteo. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina.Fil: Rodríguez, María Belén. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina.Fil: Briski, Olinda. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina.Fil: Flores Bragulat, Ana P. CONICET. Buenos Aires, Argentina. - Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Producción Equina. Río IV, Córdoba, Argentina.Fil: Demergassi, Natalia. Fundación Temaikèn. Belén de Escobar, Buenos Aires, Argentina.Fil: Losinno, Luis. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Producción Equina. Río IV, Córdoba, Argentina.Fil: Salamone, Daniel Felipe. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina.Several equids have gone extinct and many extant equids are currently considered vulnerable to critically endangered. This work aimed to evaluate whether domestic horse oocytes support preimplantation development of zebra embryos obtained by intracytoplasmic sperm injection (ICSI, zebroid) and cloning, and to study the Hippo signaling path way during the lineage specification of trophectoderm cells and inner cell mass cells. We first showed that zebra and horse sperm cells induce porcine oocyte activation and recruit maternal SMARCA4 during pronuclear formation. SMARCA4 recruitment showed to be independent of the genetic background of the injected sperm. No differences were found in blastocyst rate of ICSI hybrid (zebra spermatozoon into horse egg) embryos relative to the homospecific horse control group. Interestingly, zebra cloned blastocyst rate was significantly higher at day 8. Moreover, most ICSI and cloned horse and zebra blastocysts showed a similar expression pattern of SOX2 and nuclear YAP1 with the majority of the nuclei positive for YAP1, and most SOX2+ nuclei negative for YAP1. Here we demonstrated that horse oocytes support zebra preimplantation development of both, ICSI and cloned embryos, without compromising development to blastocyst, blastocyst cell number neither the expression of SOX2 and YAP1. Our results support the use of domestic horse oocytes as a model to study in vitro zebra embryos on behalf of preservation of valuable genetic.grafs., fot.2020articleinfo:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfdoi:10.1371/journal.pone.0238948issn:1932-6203http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2020gambiniPlos OneVol.15, no.9e0238948http://www.plos.org/reponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaenginfo:eu-repo/semantics/openAccessopenAccess2025-10-16T09:28:12Zsnrd:2020gambiniinstacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292025-10-16 09:28:13.941FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse
dc.title.none.fl_str_mv Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
title Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
spellingShingle Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
Gambini, Andrés
HORSE
GENETICS
OOCYTES
IMPLANTATION
ZEBRA
title_short Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
title_full Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
title_fullStr Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
title_full_unstemmed Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
title_sort Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
dc.creator.none.fl_str_mv Gambini, Andrés
Duque Rodríguez, Matteo
Rodríguez, María Belén
Briski, Olinda
Flores Bragulat, Ana P.
Demergassi, Natalia
Losinno, Luis
Salamone, Daniel Felipe
author Gambini, Andrés
author_facet Gambini, Andrés
Duque Rodríguez, Matteo
Rodríguez, María Belén
Briski, Olinda
Flores Bragulat, Ana P.
Demergassi, Natalia
Losinno, Luis
Salamone, Daniel Felipe
author_role author
author2 Duque Rodríguez, Matteo
Rodríguez, María Belén
Briski, Olinda
Flores Bragulat, Ana P.
Demergassi, Natalia
Losinno, Luis
Salamone, Daniel Felipe
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HORSE
GENETICS
OOCYTES
IMPLANTATION
ZEBRA
topic HORSE
GENETICS
OOCYTES
IMPLANTATION
ZEBRA
dc.description.none.fl_txt_mv Fil: Gambini, Andrés. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Producción Equina. Buenos Aires, Argentina. - CONICET. Buenos Aires, Argentina.
Fil: Duque Rodríguez, Matteo. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina.
Fil: Rodríguez, María Belén. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina.
Fil: Briski, Olinda. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina.
Fil: Flores Bragulat, Ana P. CONICET. Buenos Aires, Argentina. - Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Producción Equina. Río IV, Córdoba, Argentina.
Fil: Demergassi, Natalia. Fundación Temaikèn. Belén de Escobar, Buenos Aires, Argentina.
Fil: Losinno, Luis. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Producción Equina. Río IV, Córdoba, Argentina.
Fil: Salamone, Daniel Felipe. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina.
Several equids have gone extinct and many extant equids are currently considered vulnerable to critically endangered. This work aimed to evaluate whether domestic horse oocytes support preimplantation development of zebra embryos obtained by intracytoplasmic sperm injection (ICSI, zebroid) and cloning, and to study the Hippo signaling path way during the lineage specification of trophectoderm cells and inner cell mass cells. We first showed that zebra and horse sperm cells induce porcine oocyte activation and recruit maternal SMARCA4 during pronuclear formation. SMARCA4 recruitment showed to be independent of the genetic background of the injected sperm. No differences were found in blastocyst rate of ICSI hybrid (zebra spermatozoon into horse egg) embryos relative to the homospecific horse control group. Interestingly, zebra cloned blastocyst rate was significantly higher at day 8. Moreover, most ICSI and cloned horse and zebra blastocysts showed a similar expression pattern of SOX2 and nuclear YAP1 with the majority of the nuclei positive for YAP1, and most SOX2+ nuclei negative for YAP1. Here we demonstrated that horse oocytes support zebra preimplantation development of both, ICSI and cloned embryos, without compromising development to blastocyst, blastocyst cell number neither the expression of SOX2 and YAP1. Our results support the use of domestic horse oocytes as a model to study in vitro zebra embryos on behalf of preservation of valuable genetic.
grafs., fot.
description Fil: Gambini, Andrés. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Producción Equina. Buenos Aires, Argentina. - CONICET. Buenos Aires, Argentina.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv article
info:eu-repo/semantics/article
publishedVersion
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv doi:10.1371/journal.pone.0238948
issn:1932-6203
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2020gambini
identifier_str_mv doi:10.1371/journal.pone.0238948
issn:1932-6203
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2020gambini
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
openAccess
eu_rights_str_mv openAccess
rights_invalid_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Plos One
Vol.15, no.9
e0238948
http://www.plos.org/
reponame:FAUBA Digital (UBA-FAUBA)
instname:Universidad de Buenos Aires. Facultad de Agronomía
reponame_str FAUBA Digital (UBA-FAUBA)
collection FAUBA Digital (UBA-FAUBA)
instname_str Universidad de Buenos Aires. Facultad de Agronomía
repository.name.fl_str_mv FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía
repository.mail.fl_str_mv martino@agro.uba.ar;berasa@agro.uba.ar
_version_ 1846142981597298688
score 12.711113

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