GEF-H1 drives tumor formation, motility, invasion and metastasis in breast cancer

Autores
Fernández Chávez, Lucía; Peros, Iván Gabriel; Alonso, Exequiel Gonzalo; Facchinetti, Maria Marta; Curino, Alejandro Carlos; Colo, Georgina Pamela
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
RhoGTPases family are involved in several biological process including gene transcription, cell polarity, migration and invasion. RhoGTPases switch between on and off states and they are regulated by several GEFs (activators) and GAPs/RhoGDIs (inactivators). The aim of this work is to study the role of a particular RhoA-GEF, GEF-H1, in breast cancer (BC) progression. We observed by immunostaining a significant increase of GEF-H1 protein expression in BC human biopsies compared with non-tumoral tissue. In addition, we observed that GEF-H1 expression correlates with the invasive potential of human and murine BC cell lines. To further study the role of GEF-H1 in tumor development, we generated GEF-H1-knock out (KO) BC cells using CRISPR/Cas9 technology. We observed a decreased in proliferation, migration, invasion and anchorageindependent colony formation in GEF-H1-KO cells versus wild type (WT) cells. These results correlate with a reduced focal adhesion formation and signalling. Furthermore, BALB/c mice were subcutaneously inoculated with GEF-H1 KO cells, showing a significant delay in tumor formation and lung metastasis development compared with WT cells. These results showed that GEF-H1-RhoA activation may mediate the signalling involved in controlling cell proliferation, migration and invasion of BC cells. In vivo assays and human biopsy analyses suggest that GEF-H1 expression in BC cell might indeed contribute to tumor progression.
Fil: Fernández Chávez, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Peros, Iván Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Alonso, Exequiel Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Breast Cancer Symposium
Buenos Aires
Argentina
Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”
Universidad de Buenos Aires
Universidad Nacional de San Martín
Materia
BREAST CANCER
RHOGTPASES
GEF-H1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199379
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/199379
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling GEF-H1 drives tumor formation, motility, invasion and metastasis in breast cancerFernández Chávez, LucíaPeros, Iván GabrielAlonso, Exequiel GonzaloFacchinetti, Maria MartaCurino, Alejandro CarlosColo, Georgina PamelaBREAST CANCERRHOGTPASESGEF-H1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3RhoGTPases family are involved in several biological process including gene transcription, cell polarity, migration and invasion. RhoGTPases switch between on and off states and they are regulated by several GEFs (activators) and GAPs/RhoGDIs (inactivators). The aim of this work is to study the role of a particular RhoA-GEF, GEF-H1, in breast cancer (BC) progression. We observed by immunostaining a significant increase of GEF-H1 protein expression in BC human biopsies compared with non-tumoral tissue. In addition, we observed that GEF-H1 expression correlates with the invasive potential of human and murine BC cell lines. To further study the role of GEF-H1 in tumor development, we generated GEF-H1-knock out (KO) BC cells using CRISPR/Cas9 technology. We observed a decreased in proliferation, migration, invasion and anchorageindependent colony formation in GEF-H1-KO cells versus wild type (WT) cells. These results correlate with a reduced focal adhesion formation and signalling. Furthermore, BALB/c mice were subcutaneously inoculated with GEF-H1 KO cells, showing a significant delay in tumor formation and lung metastasis development compared with WT cells. These results showed that GEF-H1-RhoA activation may mediate the signalling involved in controlling cell proliferation, migration and invasion of BC cells. In vivo assays and human biopsy analyses suggest that GEF-H1 expression in BC cell might indeed contribute to tumor progression.Fil: Fernández Chávez, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Peros, Iván Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Alonso, Exequiel Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaBreast Cancer SymposiumBuenos AiresArgentinaCentro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”Universidad de Buenos AiresUniversidad Nacional de San MartínFundación Revista MedicinaKordon, Edith ClaudiaLanari, Claudia Lee MalvinaSimian, Marina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199379GEF-H1 drives tumor formation, motility, invasion and metastasis in breast cancer; Breast Cancer Symposium; Buenos Aires; Argentina; 2021; 16-170025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol81-21/s1/Mv81s1.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:48:58Zoai:ri.conicet.gov.ar:11336/199379instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:48:59.142CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv GEF-H1 drives tumor formation, motility, invasion and metastasis in breast cancer
title GEF-H1 drives tumor formation, motility, invasion and metastasis in breast cancer
spellingShingle GEF-H1 drives tumor formation, motility, invasion and metastasis in breast cancer
Fernández Chávez, Lucía
BREAST CANCER
RHOGTPASES
GEF-H1
title_short GEF-H1 drives tumor formation, motility, invasion and metastasis in breast cancer
title_full GEF-H1 drives tumor formation, motility, invasion and metastasis in breast cancer
title_fullStr GEF-H1 drives tumor formation, motility, invasion and metastasis in breast cancer
title_full_unstemmed GEF-H1 drives tumor formation, motility, invasion and metastasis in breast cancer
title_sort GEF-H1 drives tumor formation, motility, invasion and metastasis in breast cancer
dc.creator.none.fl_str_mv Fernández Chávez, Lucía
Peros, Iván Gabriel
Alonso, Exequiel Gonzalo
Facchinetti, Maria Marta
Curino, Alejandro Carlos
Colo, Georgina Pamela
author Fernández Chávez, Lucía
author_facet Fernández Chávez, Lucía
Peros, Iván Gabriel
Alonso, Exequiel Gonzalo
Facchinetti, Maria Marta
Curino, Alejandro Carlos
Colo, Georgina Pamela
author_role author
author2 Peros, Iván Gabriel
Alonso, Exequiel Gonzalo
Facchinetti, Maria Marta
Curino, Alejandro Carlos
Colo, Georgina Pamela
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Kordon, Edith Claudia
Lanari, Claudia Lee Malvina
Simian, Marina
dc.subject.none.fl_str_mv BREAST CANCER
RHOGTPASES
GEF-H1
topic BREAST CANCER
RHOGTPASES
GEF-H1
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv RhoGTPases family are involved in several biological process including gene transcription, cell polarity, migration and invasion. RhoGTPases switch between on and off states and they are regulated by several GEFs (activators) and GAPs/RhoGDIs (inactivators). The aim of this work is to study the role of a particular RhoA-GEF, GEF-H1, in breast cancer (BC) progression. We observed by immunostaining a significant increase of GEF-H1 protein expression in BC human biopsies compared with non-tumoral tissue. In addition, we observed that GEF-H1 expression correlates with the invasive potential of human and murine BC cell lines. To further study the role of GEF-H1 in tumor development, we generated GEF-H1-knock out (KO) BC cells using CRISPR/Cas9 technology. We observed a decreased in proliferation, migration, invasion and anchorageindependent colony formation in GEF-H1-KO cells versus wild type (WT) cells. These results correlate with a reduced focal adhesion formation and signalling. Furthermore, BALB/c mice were subcutaneously inoculated with GEF-H1 KO cells, showing a significant delay in tumor formation and lung metastasis development compared with WT cells. These results showed that GEF-H1-RhoA activation may mediate the signalling involved in controlling cell proliferation, migration and invasion of BC cells. In vivo assays and human biopsy analyses suggest that GEF-H1 expression in BC cell might indeed contribute to tumor progression.
Fil: Fernández Chávez, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Peros, Iván Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Alonso, Exequiel Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Colo, Georgina Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Breast Cancer Symposium
Buenos Aires
Argentina
Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”
Universidad de Buenos Aires
Universidad Nacional de San Martín
description RhoGTPases family are involved in several biological process including gene transcription, cell polarity, migration and invasion. RhoGTPases switch between on and off states and they are regulated by several GEFs (activators) and GAPs/RhoGDIs (inactivators). The aim of this work is to study the role of a particular RhoA-GEF, GEF-H1, in breast cancer (BC) progression. We observed by immunostaining a significant increase of GEF-H1 protein expression in BC human biopsies compared with non-tumoral tissue. In addition, we observed that GEF-H1 expression correlates with the invasive potential of human and murine BC cell lines. To further study the role of GEF-H1 in tumor development, we generated GEF-H1-knock out (KO) BC cells using CRISPR/Cas9 technology. We observed a decreased in proliferation, migration, invasion and anchorageindependent colony formation in GEF-H1-KO cells versus wild type (WT) cells. These results correlate with a reduced focal adhesion formation and signalling. Furthermore, BALB/c mice were subcutaneously inoculated with GEF-H1 KO cells, showing a significant delay in tumor formation and lung metastasis development compared with WT cells. These results showed that GEF-H1-RhoA activation may mediate the signalling involved in controlling cell proliferation, migration and invasion of BC cells. In vivo assays and human biopsy analyses suggest that GEF-H1 expression in BC cell might indeed contribute to tumor progression.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Simposio
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199379
GEF-H1 drives tumor formation, motility, invasion and metastasis in breast cancer; Breast Cancer Symposium; Buenos Aires; Argentina; 2021; 16-17
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199379
identifier_str_mv GEF-H1 drives tumor formation, motility, invasion and metastasis in breast cancer; Breast Cancer Symposium; Buenos Aires; Argentina; 2021; 16-17
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol81-21/s1/Mv81s1.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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