Rat liver antioxidant response to iron and copper overloads
- Autores
- Musacco Sebio, Rosario Natalia; Saporito Magriñá, Christian Martín; Semprine, Jimena Vanina; Torti, Horacio Emilio; Ferrarotti, Nidia Fatima; Castro-Parodi, Mauricio Omar; Damiano, Alicia Ermelinda; Boveris, Alberto Antonio; Repetto, Marisa Gabriela
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The rat liver antioxidant response to Fe and Cu overloads (0–60 mg/kg) was studied. Dose- and time-responses were determined and summarized by t1/2 and C50, the time and the liver metal content for half maximal oxidative responses. Liver GSH (reduced glutathione) and GSSG (glutathione disulfide) were determined. The GSH content and the GSH/GSSG ratio markedly decreased after Fe (58–66%) and Cu (79–81%) loads, with t1/2 of 4.0 and 2.0 h. The C50 were in a similar range for all the indicators (110–124 μg Fe/g and 40–50 μg Cu/g) and suggest a unique free-radical mediated process. Hydrophilic antioxidants markedly decreased after Fe and Cu (60–75%; t1/2: 4.5 and 4.0 h). Lipophilic antioxidants were also decreased (30–92%; t1/2: 7.0 and 5.5 h) after Fe and Cu. Superoxide dismutase (SOD) activities (Cu,Zn-SOD and Mn-SOD) and protein expression were adaptively increased after metal overloads (Cu,Zn-SOD: t1/2: 8–8.5 h and Mn-SOD: t1/2: 8.5–8.0 h). Catalase activity was increased after Fe (65%; t1/2: 8.5 h) and decreased after Cu (26%; t1/2: 8.0 h), whereas catalase expression was increased after Fe and decreased after Cu overloads. Glutathione peroxidase activity decreased after metal loads by 22–39% with a t1/2 of 4.5 h and with unchanged protein expression. GSH is the main and fastest responder antioxidant in Fe and Cu overloads. The results indicate that thiol (SH) content and antioxidant enzyme activities are central to the antioxidant defense in the oxidative stress and damage after Fe and Cu overloads.
Fil: Musacco Sebio, Rosario Natalia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Saporito Magriñá, Christian Martín. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Semprine, Jimena Vanina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Torti, Horacio Emilio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Ferrarotti, Nidia Fatima. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Castro-Parodi, Mauricio Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Damiano, Alicia Ermelinda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Boveris, Alberto Antonio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Repetto, Marisa Gabriela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina - Materia
-
Antioxidants
Liver Chemiluminescence
Copper
Glutathione
Iron
Oxidative Damage - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/30521
Ver los metadatos del registro completo
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Rat liver antioxidant response to iron and copper overloadsMusacco Sebio, Rosario NataliaSaporito Magriñá, Christian MartínSemprine, Jimena VaninaTorti, Horacio EmilioFerrarotti, Nidia FatimaCastro-Parodi, Mauricio OmarDamiano, Alicia ErmelindaBoveris, Alberto AntonioRepetto, Marisa GabrielaAntioxidantsLiver ChemiluminescenceCopperGlutathioneIronOxidative Damagehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The rat liver antioxidant response to Fe and Cu overloads (0–60 mg/kg) was studied. Dose- and time-responses were determined and summarized by t1/2 and C50, the time and the liver metal content for half maximal oxidative responses. Liver GSH (reduced glutathione) and GSSG (glutathione disulfide) were determined. The GSH content and the GSH/GSSG ratio markedly decreased after Fe (58–66%) and Cu (79–81%) loads, with t1/2 of 4.0 and 2.0 h. The C50 were in a similar range for all the indicators (110–124 μg Fe/g and 40–50 μg Cu/g) and suggest a unique free-radical mediated process. Hydrophilic antioxidants markedly decreased after Fe and Cu (60–75%; t1/2: 4.5 and 4.0 h). Lipophilic antioxidants were also decreased (30–92%; t1/2: 7.0 and 5.5 h) after Fe and Cu. Superoxide dismutase (SOD) activities (Cu,Zn-SOD and Mn-SOD) and protein expression were adaptively increased after metal overloads (Cu,Zn-SOD: t1/2: 8–8.5 h and Mn-SOD: t1/2: 8.5–8.0 h). Catalase activity was increased after Fe (65%; t1/2: 8.5 h) and decreased after Cu (26%; t1/2: 8.0 h), whereas catalase expression was increased after Fe and decreased after Cu overloads. Glutathione peroxidase activity decreased after metal loads by 22–39% with a t1/2 of 4.5 h and with unchanged protein expression. GSH is the main and fastest responder antioxidant in Fe and Cu overloads. The results indicate that thiol (SH) content and antioxidant enzyme activities are central to the antioxidant defense in the oxidative stress and damage after Fe and Cu overloads.Fil: Musacco Sebio, Rosario Natalia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Saporito Magriñá, Christian Martín. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Semprine, Jimena Vanina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Torti, Horacio Emilio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Ferrarotti, Nidia Fatima. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Castro-Parodi, Mauricio Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Damiano, Alicia Ermelinda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Boveris, Alberto Antonio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Repetto, Marisa Gabriela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaElsevier2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30521Musacco Sebio, Rosario Natalia; Saporito Magriñá, Christian Martín; Semprine, Jimena Vanina; Torti, Horacio Emilio; Ferrarotti, Nidia Fatima; et al.; Rat liver antioxidant response to iron and copper overloads; Elsevier; Journal of Inorganic Biochemistry; 137; 4-2014; 94-1000162-0134CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi//10.1016/j.jinorgbio.2014.04.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:45Zoai:ri.conicet.gov.ar:11336/30521instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:45.299CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Rat liver antioxidant response to iron and copper overloads |
title |
Rat liver antioxidant response to iron and copper overloads |
spellingShingle |
Rat liver antioxidant response to iron and copper overloads Musacco Sebio, Rosario Natalia Antioxidants Liver Chemiluminescence Copper Glutathione Iron Oxidative Damage |
title_short |
Rat liver antioxidant response to iron and copper overloads |
title_full |
Rat liver antioxidant response to iron and copper overloads |
title_fullStr |
Rat liver antioxidant response to iron and copper overloads |
title_full_unstemmed |
Rat liver antioxidant response to iron and copper overloads |
title_sort |
Rat liver antioxidant response to iron and copper overloads |
dc.creator.none.fl_str_mv |
Musacco Sebio, Rosario Natalia Saporito Magriñá, Christian Martín Semprine, Jimena Vanina Torti, Horacio Emilio Ferrarotti, Nidia Fatima Castro-Parodi, Mauricio Omar Damiano, Alicia Ermelinda Boveris, Alberto Antonio Repetto, Marisa Gabriela |
author |
Musacco Sebio, Rosario Natalia |
author_facet |
Musacco Sebio, Rosario Natalia Saporito Magriñá, Christian Martín Semprine, Jimena Vanina Torti, Horacio Emilio Ferrarotti, Nidia Fatima Castro-Parodi, Mauricio Omar Damiano, Alicia Ermelinda Boveris, Alberto Antonio Repetto, Marisa Gabriela |
author_role |
author |
author2 |
Saporito Magriñá, Christian Martín Semprine, Jimena Vanina Torti, Horacio Emilio Ferrarotti, Nidia Fatima Castro-Parodi, Mauricio Omar Damiano, Alicia Ermelinda Boveris, Alberto Antonio Repetto, Marisa Gabriela |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Antioxidants Liver Chemiluminescence Copper Glutathione Iron Oxidative Damage |
topic |
Antioxidants Liver Chemiluminescence Copper Glutathione Iron Oxidative Damage |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The rat liver antioxidant response to Fe and Cu overloads (0–60 mg/kg) was studied. Dose- and time-responses were determined and summarized by t1/2 and C50, the time and the liver metal content for half maximal oxidative responses. Liver GSH (reduced glutathione) and GSSG (glutathione disulfide) were determined. The GSH content and the GSH/GSSG ratio markedly decreased after Fe (58–66%) and Cu (79–81%) loads, with t1/2 of 4.0 and 2.0 h. The C50 were in a similar range for all the indicators (110–124 μg Fe/g and 40–50 μg Cu/g) and suggest a unique free-radical mediated process. Hydrophilic antioxidants markedly decreased after Fe and Cu (60–75%; t1/2: 4.5 and 4.0 h). Lipophilic antioxidants were also decreased (30–92%; t1/2: 7.0 and 5.5 h) after Fe and Cu. Superoxide dismutase (SOD) activities (Cu,Zn-SOD and Mn-SOD) and protein expression were adaptively increased after metal overloads (Cu,Zn-SOD: t1/2: 8–8.5 h and Mn-SOD: t1/2: 8.5–8.0 h). Catalase activity was increased after Fe (65%; t1/2: 8.5 h) and decreased after Cu (26%; t1/2: 8.0 h), whereas catalase expression was increased after Fe and decreased after Cu overloads. Glutathione peroxidase activity decreased after metal loads by 22–39% with a t1/2 of 4.5 h and with unchanged protein expression. GSH is the main and fastest responder antioxidant in Fe and Cu overloads. The results indicate that thiol (SH) content and antioxidant enzyme activities are central to the antioxidant defense in the oxidative stress and damage after Fe and Cu overloads. Fil: Musacco Sebio, Rosario Natalia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Saporito Magriñá, Christian Martín. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Semprine, Jimena Vanina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Torti, Horacio Emilio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Ferrarotti, Nidia Fatima. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Castro-Parodi, Mauricio Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Damiano, Alicia Ermelinda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Boveris, Alberto Antonio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química General e Inorgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Repetto, Marisa Gabriela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina |
description |
The rat liver antioxidant response to Fe and Cu overloads (0–60 mg/kg) was studied. Dose- and time-responses were determined and summarized by t1/2 and C50, the time and the liver metal content for half maximal oxidative responses. Liver GSH (reduced glutathione) and GSSG (glutathione disulfide) were determined. The GSH content and the GSH/GSSG ratio markedly decreased after Fe (58–66%) and Cu (79–81%) loads, with t1/2 of 4.0 and 2.0 h. The C50 were in a similar range for all the indicators (110–124 μg Fe/g and 40–50 μg Cu/g) and suggest a unique free-radical mediated process. Hydrophilic antioxidants markedly decreased after Fe and Cu (60–75%; t1/2: 4.5 and 4.0 h). Lipophilic antioxidants were also decreased (30–92%; t1/2: 7.0 and 5.5 h) after Fe and Cu. Superoxide dismutase (SOD) activities (Cu,Zn-SOD and Mn-SOD) and protein expression were adaptively increased after metal overloads (Cu,Zn-SOD: t1/2: 8–8.5 h and Mn-SOD: t1/2: 8.5–8.0 h). Catalase activity was increased after Fe (65%; t1/2: 8.5 h) and decreased after Cu (26%; t1/2: 8.0 h), whereas catalase expression was increased after Fe and decreased after Cu overloads. Glutathione peroxidase activity decreased after metal loads by 22–39% with a t1/2 of 4.5 h and with unchanged protein expression. GSH is the main and fastest responder antioxidant in Fe and Cu overloads. The results indicate that thiol (SH) content and antioxidant enzyme activities are central to the antioxidant defense in the oxidative stress and damage after Fe and Cu overloads. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/30521 Musacco Sebio, Rosario Natalia; Saporito Magriñá, Christian Martín; Semprine, Jimena Vanina; Torti, Horacio Emilio; Ferrarotti, Nidia Fatima; et al.; Rat liver antioxidant response to iron and copper overloads; Elsevier; Journal of Inorganic Biochemistry; 137; 4-2014; 94-100 0162-0134 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/30521 |
identifier_str_mv |
Musacco Sebio, Rosario Natalia; Saporito Magriñá, Christian Martín; Semprine, Jimena Vanina; Torti, Horacio Emilio; Ferrarotti, Nidia Fatima; et al.; Rat liver antioxidant response to iron and copper overloads; Elsevier; Journal of Inorganic Biochemistry; 137; 4-2014; 94-100 0162-0134 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/ info:eu-repo/semantics/altIdentifier/doi//10.1016/j.jinorgbio.2014.04.014 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |