Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage

Autores
Santo Orihuela, Pablo Luis; Carvajal, Guillermo; Picollo, María Inés; Vassena, Claudia Viviana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present work was to study the deltamethrin susceptibility of eggs from populations of Triatoma infestans and contribution of pyrethroid esterases to its degradation. Insects were from sylvatic areas Veinte de Octubre and Kirus-Mayu (Bolivia) and from domiciliary areas El Palmar (Bolivia) and La Pista (Argentina). Susceptibility was determined by dose-response bioassays. Serial dilutions of deltamethrin (0.0005 to 1 mg/ml) were topically applied on 12 days old eggs. El Palmar showed the highest Lethal Doses Ratio (LDR) value (44.90) compared with NFS reference strain whereas Veinte de Octubre showed the lowest value (0.50). Pyrethroid esterases were evaluated using 7-coumaryl permethrate (7-CP) on individually homogenized eggs of each population and NFS. El Palmar and La Pista showed values of 40.11 and 36.64 pmol/min/mg protein, statistically similar to NFS (34.92 pmol/min/mg protein) and different from Kirus-Mayu and Veinte de Octubre (27.49 and 22.69 pmol/min/mg protein). The toxicological data indicate that the domestic populations are resistant to deltamethrin but no statistical contribution of 7-CP esterases was observed. Sylvatic populations showed values of LDR similar to NFS but lower activities of 7-CP esterases. This is the first study of pyrethroid esterases on eggs of Triatoma infestans using a specific substrate (7-CP).
Fil: Santo Orihuela, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina;
Fil: Carvajal, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina;
Fil: Picollo, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina;
Fil: Vassena, Claudia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina;
Fuente
www.scielo.br
Materia
TRIATOMA INFESTANS
EGG
PYRETHROID INSECTICIDES
PYRETHROID ESTERASES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1856
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/1856
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stageSanto Orihuela, Pablo LuisCarvajal, GuillermoPicollo, María InésVassena, Claudia VivianaTRIATOMA INFESTANSEGGPYRETHROID INSECTICIDESPYRETHROID ESTERASEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of the present work was to study the deltamethrin susceptibility of eggs from populations of Triatoma infestans and contribution of pyrethroid esterases to its degradation. Insects were from sylvatic areas Veinte de Octubre and Kirus-Mayu (Bolivia) and from domiciliary areas El Palmar (Bolivia) and La Pista (Argentina). Susceptibility was determined by dose-response bioassays. Serial dilutions of deltamethrin (0.0005 to 1 mg/ml) were topically applied on 12 days old eggs. El Palmar showed the highest Lethal Doses Ratio (LDR) value (44.90) compared with NFS reference strain whereas Veinte de Octubre showed the lowest value (0.50). Pyrethroid esterases were evaluated using 7-coumaryl permethrate (7-CP) on individually homogenized eggs of each population and NFS. El Palmar and La Pista showed values of 40.11 and 36.64 pmol/min/mg protein, statistically similar to NFS (34.92 pmol/min/mg protein) and different from Kirus-Mayu and Veinte de Octubre (27.49 and 22.69 pmol/min/mg protein). The toxicological data indicate that the domestic populations are resistant to deltamethrin but no statistical contribution of 7-CP esterases was observed. Sylvatic populations showed values of LDR similar to NFS but lower activities of 7-CP esterases. This is the first study of pyrethroid esterases on eggs of Triatoma infestans using a specific substrate (7-CP).Fil: Santo Orihuela, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina;Fil: Carvajal, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina;Fil: Picollo, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina;Fil: Vassena, Claudia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina;Fundacao Oswaldo Cruz2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1856Santo Orihuela, Pablo Luis; Carvajal, Guillermo; Picollo, María Inés; Vassena, Claudia Viviana; Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage; Fundacao Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz.; 108; 8; 12-2013; 1031-10360074-02761678-8060www.scielo.brreponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicasenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/0074-0276130184info:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/pdf/mioc/v108n8/0074-0276-mioc-108-8-1031.pdfinfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4005558/pdf/0074-0276-mioc-108-08-01031.pdfinfo:eu-repo/semantics/altIdentifier/url/http://memorias.ioc.fiocruz.br/issues/current-issue/item/1599-0184_analysing-deltamethrin-susceptibility-and-pyrethroid-esterase-activity-variations-in-sylvatic-and-domestic-triatoma-infestans-at-the-embryonic-stageinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/2025-09-03T10:03:43Zoai:ri.conicet.gov.ar:11336/1856instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:44.067CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage
title Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage
spellingShingle Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage
Santo Orihuela, Pablo Luis
TRIATOMA INFESTANS
EGG
PYRETHROID INSECTICIDES
PYRETHROID ESTERASES
title_short Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage
title_full Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage
title_fullStr Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage
title_full_unstemmed Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage
title_sort Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage
dc.creator.none.fl_str_mv Santo Orihuela, Pablo Luis
Carvajal, Guillermo
Picollo, María Inés
Vassena, Claudia Viviana
author Santo Orihuela, Pablo Luis
author_facet Santo Orihuela, Pablo Luis
Carvajal, Guillermo
Picollo, María Inés
Vassena, Claudia Viviana
author_role author
author2 Carvajal, Guillermo
Picollo, María Inés
Vassena, Claudia Viviana
author2_role author
author
author
dc.subject.none.fl_str_mv TRIATOMA INFESTANS
EGG
PYRETHROID INSECTICIDES
PYRETHROID ESTERASES
topic TRIATOMA INFESTANS
EGG
PYRETHROID INSECTICIDES
PYRETHROID ESTERASES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of the present work was to study the deltamethrin susceptibility of eggs from populations of Triatoma infestans and contribution of pyrethroid esterases to its degradation. Insects were from sylvatic areas Veinte de Octubre and Kirus-Mayu (Bolivia) and from domiciliary areas El Palmar (Bolivia) and La Pista (Argentina). Susceptibility was determined by dose-response bioassays. Serial dilutions of deltamethrin (0.0005 to 1 mg/ml) were topically applied on 12 days old eggs. El Palmar showed the highest Lethal Doses Ratio (LDR) value (44.90) compared with NFS reference strain whereas Veinte de Octubre showed the lowest value (0.50). Pyrethroid esterases were evaluated using 7-coumaryl permethrate (7-CP) on individually homogenized eggs of each population and NFS. El Palmar and La Pista showed values of 40.11 and 36.64 pmol/min/mg protein, statistically similar to NFS (34.92 pmol/min/mg protein) and different from Kirus-Mayu and Veinte de Octubre (27.49 and 22.69 pmol/min/mg protein). The toxicological data indicate that the domestic populations are resistant to deltamethrin but no statistical contribution of 7-CP esterases was observed. Sylvatic populations showed values of LDR similar to NFS but lower activities of 7-CP esterases. This is the first study of pyrethroid esterases on eggs of Triatoma infestans using a specific substrate (7-CP).
Fil: Santo Orihuela, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina;
Fil: Carvajal, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina;
Fil: Picollo, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina;
Fil: Vassena, Claudia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina;
description The aim of the present work was to study the deltamethrin susceptibility of eggs from populations of Triatoma infestans and contribution of pyrethroid esterases to its degradation. Insects were from sylvatic areas Veinte de Octubre and Kirus-Mayu (Bolivia) and from domiciliary areas El Palmar (Bolivia) and La Pista (Argentina). Susceptibility was determined by dose-response bioassays. Serial dilutions of deltamethrin (0.0005 to 1 mg/ml) were topically applied on 12 days old eggs. El Palmar showed the highest Lethal Doses Ratio (LDR) value (44.90) compared with NFS reference strain whereas Veinte de Octubre showed the lowest value (0.50). Pyrethroid esterases were evaluated using 7-coumaryl permethrate (7-CP) on individually homogenized eggs of each population and NFS. El Palmar and La Pista showed values of 40.11 and 36.64 pmol/min/mg protein, statistically similar to NFS (34.92 pmol/min/mg protein) and different from Kirus-Mayu and Veinte de Octubre (27.49 and 22.69 pmol/min/mg protein). The toxicological data indicate that the domestic populations are resistant to deltamethrin but no statistical contribution of 7-CP esterases was observed. Sylvatic populations showed values of LDR similar to NFS but lower activities of 7-CP esterases. This is the first study of pyrethroid esterases on eggs of Triatoma infestans using a specific substrate (7-CP).
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1856
Santo Orihuela, Pablo Luis; Carvajal, Guillermo; Picollo, María Inés; Vassena, Claudia Viviana; Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage; Fundacao Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz.; 108; 8; 12-2013; 1031-1036
0074-0276
1678-8060
url http://hdl.handle.net/11336/1856
identifier_str_mv Santo Orihuela, Pablo Luis; Carvajal, Guillermo; Picollo, María Inés; Vassena, Claudia Viviana; Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage; Fundacao Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz.; 108; 8; 12-2013; 1031-1036
0074-0276
1678-8060
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/0074-0276130184
info:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/pdf/mioc/v108n8/0074-0276-mioc-108-8-1031.pdf
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4005558/pdf/0074-0276-mioc-108-08-01031.pdf
info:eu-repo/semantics/altIdentifier/url/http://memorias.ioc.fiocruz.br/issues/current-issue/item/1599-0184_analysing-deltamethrin-susceptibility-and-pyrethroid-esterase-activity-variations-in-sylvatic-and-domestic-triatoma-infestans-at-the-embryonic-stage
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Fundacao Oswaldo Cruz
publisher.none.fl_str_mv Fundacao Oswaldo Cruz
dc.source.none.fl_str_mv www.scielo.br
reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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