Long-term treatment with methanandamide attenuates LPS-induced periodontitis in rats

Autores
Ossola, César Ángel; Surkin, Pablo Nicolas; Pugnaloni, Antonela; Mohn, Claudia Ester; Elverdin, Juan Carlos; Fernández Solari, Jose Javier
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
OBJECTIVE: Evidence exists of the anti-inflammatory and immunological properties of endocannabinoids in various tissues; the aim of the present study was therefore to assess the effect of long-term treatment with the synthetic cannabinoid methanandamide (Meth-AEA) on the progression of periodontitis in rats. MATERIALS AND METHODS: Periodontitis was induced by injecting LPS (1 mg/ml) into the gingiva around the neck of the first upper and lower molars, and into the inter-dental space between the first and second molars. This protocol was repeated for 6 weeks on days 1, 3, and 5 of each week. RESULTS: Long-term treatment with topical Meth-AEA (500 ng/ml), applied daily to gingival tissue of rats induced with periodontitis, significantly diminished the alveolar bone loss, measured as the distance between the cemento-enamel junction and the alveolar crest, in both maxillary and mandibular first molars, compared to rats without treatment (P < 0.05). The treatment also reduced the production of some biological mediators of periodontal disease augmented by LPS, such as tumor necrosis factor alpha (from 119.4 ± 9.9 pg/mg protein to 75.1 ± 10.8, P < 0.05) and nitric oxide produced by inducible nitric oxide synthase (from 507.7 ± 107.1 pmol/min/mg protein to 163.1 ± 53.9, P < 0.01). CONCLUSION: These results demonstrate the beneficial effects of treatment with Meth-AEA on gingival tissue of rats with periodontitis.
Fil: Ossola, César Ángel. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Surkin, Pablo Nicolas. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pugnaloni, Antonela. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Mohn, Claudia Ester. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Elverdin, Juan Carlos. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Fernández Solari, Jose Javier. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Oxygen Radicals
Lipopolysaccharide
Bone Injury
Tumor Necrosis Factor
Prostaglandins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16385
Acceder
id CONICETDig_ffb49e1e0394a13885d0e1b6bf409483
oai_identifier_str oai:ri.conicet.gov.ar:11336/16385
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Long-term treatment with methanandamide attenuates LPS-induced periodontitis in ratsOssola, César ÁngelSurkin, Pablo NicolasPugnaloni, AntonelaMohn, Claudia EsterElverdin, Juan CarlosFernández Solari, Jose JavierOxygen RadicalsLipopolysaccharideBone InjuryTumor Necrosis FactorProstaglandinshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3OBJECTIVE: Evidence exists of the anti-inflammatory and immunological properties of endocannabinoids in various tissues; the aim of the present study was therefore to assess the effect of long-term treatment with the synthetic cannabinoid methanandamide (Meth-AEA) on the progression of periodontitis in rats. MATERIALS AND METHODS: Periodontitis was induced by injecting LPS (1 mg/ml) into the gingiva around the neck of the first upper and lower molars, and into the inter-dental space between the first and second molars. This protocol was repeated for 6 weeks on days 1, 3, and 5 of each week. RESULTS: Long-term treatment with topical Meth-AEA (500 ng/ml), applied daily to gingival tissue of rats induced with periodontitis, significantly diminished the alveolar bone loss, measured as the distance between the cemento-enamel junction and the alveolar crest, in both maxillary and mandibular first molars, compared to rats without treatment (P < 0.05). The treatment also reduced the production of some biological mediators of periodontal disease augmented by LPS, such as tumor necrosis factor alpha (from 119.4 ± 9.9 pg/mg protein to 75.1 ± 10.8, P < 0.05) and nitric oxide produced by inducible nitric oxide synthase (from 507.7 ± 107.1 pmol/min/mg protein to 163.1 ± 53.9, P < 0.01). CONCLUSION: These results demonstrate the beneficial effects of treatment with Meth-AEA on gingival tissue of rats with periodontitis.Fil: Ossola, César Ángel. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Surkin, Pablo Nicolas. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pugnaloni, Antonela. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Mohn, Claudia Ester. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Elverdin, Juan Carlos. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Fernández Solari, Jose Javier. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBirkhauser Verlag Ag2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16385Ossola, César Ángel; Surkin, Pablo Nicolas; Pugnaloni, Antonela; Mohn, Claudia Ester; Elverdin, Juan Carlos; et al.; Long-term treatment with methanandamide attenuates LPS-induced periodontitis in rats; Birkhauser Verlag Ag; Inflammation Research; 61; 9; 9-2012; 941-9481023-38301420-908Xenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00011-012-0485-zinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00011-012-0485-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:24Zoai:ri.conicet.gov.ar:11336/16385instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:24.903CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Long-term treatment with methanandamide attenuates LPS-induced periodontitis in rats
title Long-term treatment with methanandamide attenuates LPS-induced periodontitis in rats
spellingShingle Long-term treatment with methanandamide attenuates LPS-induced periodontitis in rats
Ossola, César Ángel
Oxygen Radicals
Lipopolysaccharide
Bone Injury
Tumor Necrosis Factor
Prostaglandins
title_short Long-term treatment with methanandamide attenuates LPS-induced periodontitis in rats
title_full Long-term treatment with methanandamide attenuates LPS-induced periodontitis in rats
title_fullStr Long-term treatment with methanandamide attenuates LPS-induced periodontitis in rats
title_full_unstemmed Long-term treatment with methanandamide attenuates LPS-induced periodontitis in rats
title_sort Long-term treatment with methanandamide attenuates LPS-induced periodontitis in rats
dc.creator.none.fl_str_mv Ossola, César Ángel
Surkin, Pablo Nicolas
Pugnaloni, Antonela
Mohn, Claudia Ester
Elverdin, Juan Carlos
Fernández Solari, Jose Javier
author Ossola, César Ángel
author_facet Ossola, César Ángel
Surkin, Pablo Nicolas
Pugnaloni, Antonela
Mohn, Claudia Ester
Elverdin, Juan Carlos
Fernández Solari, Jose Javier
author_role author
author2 Surkin, Pablo Nicolas
Pugnaloni, Antonela
Mohn, Claudia Ester
Elverdin, Juan Carlos
Fernández Solari, Jose Javier
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Oxygen Radicals
Lipopolysaccharide
Bone Injury
Tumor Necrosis Factor
Prostaglandins
topic Oxygen Radicals
Lipopolysaccharide
Bone Injury
Tumor Necrosis Factor
Prostaglandins
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv OBJECTIVE: Evidence exists of the anti-inflammatory and immunological properties of endocannabinoids in various tissues; the aim of the present study was therefore to assess the effect of long-term treatment with the synthetic cannabinoid methanandamide (Meth-AEA) on the progression of periodontitis in rats. MATERIALS AND METHODS: Periodontitis was induced by injecting LPS (1 mg/ml) into the gingiva around the neck of the first upper and lower molars, and into the inter-dental space between the first and second molars. This protocol was repeated for 6 weeks on days 1, 3, and 5 of each week. RESULTS: Long-term treatment with topical Meth-AEA (500 ng/ml), applied daily to gingival tissue of rats induced with periodontitis, significantly diminished the alveolar bone loss, measured as the distance between the cemento-enamel junction and the alveolar crest, in both maxillary and mandibular first molars, compared to rats without treatment (P < 0.05). The treatment also reduced the production of some biological mediators of periodontal disease augmented by LPS, such as tumor necrosis factor alpha (from 119.4 ± 9.9 pg/mg protein to 75.1 ± 10.8, P < 0.05) and nitric oxide produced by inducible nitric oxide synthase (from 507.7 ± 107.1 pmol/min/mg protein to 163.1 ± 53.9, P < 0.01). CONCLUSION: These results demonstrate the beneficial effects of treatment with Meth-AEA on gingival tissue of rats with periodontitis.
Fil: Ossola, César Ángel. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Surkin, Pablo Nicolas. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pugnaloni, Antonela. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Mohn, Claudia Ester. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Elverdin, Juan Carlos. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Fernández Solari, Jose Javier. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description OBJECTIVE: Evidence exists of the anti-inflammatory and immunological properties of endocannabinoids in various tissues; the aim of the present study was therefore to assess the effect of long-term treatment with the synthetic cannabinoid methanandamide (Meth-AEA) on the progression of periodontitis in rats. MATERIALS AND METHODS: Periodontitis was induced by injecting LPS (1 mg/ml) into the gingiva around the neck of the first upper and lower molars, and into the inter-dental space between the first and second molars. This protocol was repeated for 6 weeks on days 1, 3, and 5 of each week. RESULTS: Long-term treatment with topical Meth-AEA (500 ng/ml), applied daily to gingival tissue of rats induced with periodontitis, significantly diminished the alveolar bone loss, measured as the distance between the cemento-enamel junction and the alveolar crest, in both maxillary and mandibular first molars, compared to rats without treatment (P < 0.05). The treatment also reduced the production of some biological mediators of periodontal disease augmented by LPS, such as tumor necrosis factor alpha (from 119.4 ± 9.9 pg/mg protein to 75.1 ± 10.8, P < 0.05) and nitric oxide produced by inducible nitric oxide synthase (from 507.7 ± 107.1 pmol/min/mg protein to 163.1 ± 53.9, P < 0.01). CONCLUSION: These results demonstrate the beneficial effects of treatment with Meth-AEA on gingival tissue of rats with periodontitis.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16385
Ossola, César Ángel; Surkin, Pablo Nicolas; Pugnaloni, Antonela; Mohn, Claudia Ester; Elverdin, Juan Carlos; et al.; Long-term treatment with methanandamide attenuates LPS-induced periodontitis in rats; Birkhauser Verlag Ag; Inflammation Research; 61; 9; 9-2012; 941-948
1023-3830
1420-908X
url http://hdl.handle.net/11336/16385
identifier_str_mv Ossola, César Ángel; Surkin, Pablo Nicolas; Pugnaloni, Antonela; Mohn, Claudia Ester; Elverdin, Juan Carlos; et al.; Long-term treatment with methanandamide attenuates LPS-induced periodontitis in rats; Birkhauser Verlag Ag; Inflammation Research; 61; 9; 9-2012; 941-948
1023-3830
1420-908X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00011-012-0485-z
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00011-012-0485-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Birkhauser Verlag Ag
publisher.none.fl_str_mv Birkhauser Verlag Ag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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