The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection

Autores
Corral, Jordi; Rocafort, Mercedes; Ripa, Maria Belén; Vandecaveye, Agustina Irene; Pezzoni, Magdalena; Invernon, Alicia; Coll, Núria S.; Valls, Marc; Orellano, Elena Graciela
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Throughout their life cycle, pathogens are challenged by Reactive Oxygen Species (ROS) and must deploy defence mechanisms against oxidative damage. Environmental stressors such as ultraviolet radiation and desiccation induce intracellular ROS production. Moreover, during pathogen colonisation, plant hosts initiate an oxidative burst, leading to the accumulation of hydrogen peroxide (H2O2). Catalases mitigate ROS toxicity by catalysing the breakdown of H2O2 into water and oxygen. In this study, we explored the roles of the monofunctional catalase (KatE) and the bifunctional catalase-peroxidase (KatG) in the phytopathogen Ralstonia solanacearum during both environmental stress conditions and in planta colonisation stages. Our results demonstrate that both catalases are critical for surviving ROS, with KatG having a more preponderant role. Plant pathogenicity assays show that catalases are not required for virulence although they are important for bacterial fitness in the apoplast. In contrast, we prove that both catalases are vital for R. solanacearum response to oxidative stress in the external environment. KatG was indispensable for survival in soil, as well as for enduring ultraviolet radiation and desiccation exposure. Our findings reveal that the primary function of R. solanacearum catalases is to counteract ROS generated by environmental stressors rather than host-derived oxidative defences. This research identifies specific environmental stressors that these catalases combat during pathogen survival. These insights pave the way for future strategies targeting catalase activity to control bacterial wilt disease.
Fil: Corral, Jordi. Universitat Autònoma de Barcelona; España
Fil: Rocafort, Mercedes. Universitat Autònoma de Barcelona; España
Fil: Ripa, Maria Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Vandecaveye, Agustina Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Pezzoni, Magdalena. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Invernon, Alicia. Universitat Autònoma de Barcelona; España
Fil: Coll, Núria S.. Universitat Autònoma de Barcelona; España
Fil: Valls, Marc. Universitat Autònoma de Barcelona; España
Fil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
Ralstonia solanacearum
catalases
Reactive oxygen species (ROS)
Environmental stressors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/277358
Acceder
id CONICETDig_feb30ff974412ce7b02ffa352a6594e1
oai_identifier_str oai:ri.conicet.gov.ar:11336/277358
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infectionCorral, JordiRocafort, MercedesRipa, Maria BelénVandecaveye, Agustina IrenePezzoni, MagdalenaInvernon, AliciaColl, Núria S.Valls, MarcOrellano, Elena GracielaRalstonia solanacearumcatalasesReactive oxygen species (ROS)Environmental stressorshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Throughout their life cycle, pathogens are challenged by Reactive Oxygen Species (ROS) and must deploy defence mechanisms against oxidative damage. Environmental stressors such as ultraviolet radiation and desiccation induce intracellular ROS production. Moreover, during pathogen colonisation, plant hosts initiate an oxidative burst, leading to the accumulation of hydrogen peroxide (H2O2). Catalases mitigate ROS toxicity by catalysing the breakdown of H2O2 into water and oxygen. In this study, we explored the roles of the monofunctional catalase (KatE) and the bifunctional catalase-peroxidase (KatG) in the phytopathogen Ralstonia solanacearum during both environmental stress conditions and in planta colonisation stages. Our results demonstrate that both catalases are critical for surviving ROS, with KatG having a more preponderant role. Plant pathogenicity assays show that catalases are not required for virulence although they are important for bacterial fitness in the apoplast. In contrast, we prove that both catalases are vital for R. solanacearum response to oxidative stress in the external environment. KatG was indispensable for survival in soil, as well as for enduring ultraviolet radiation and desiccation exposure. Our findings reveal that the primary function of R. solanacearum catalases is to counteract ROS generated by environmental stressors rather than host-derived oxidative defences. This research identifies specific environmental stressors that these catalases combat during pathogen survival. These insights pave the way for future strategies targeting catalase activity to control bacterial wilt disease.Fil: Corral, Jordi. Universitat Autònoma de Barcelona; EspañaFil: Rocafort, Mercedes. Universitat Autònoma de Barcelona; EspañaFil: Ripa, Maria Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Vandecaveye, Agustina Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Pezzoni, Magdalena. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Invernon, Alicia. Universitat Autònoma de Barcelona; EspañaFil: Coll, Núria S.. Universitat Autònoma de Barcelona; EspañaFil: Valls, Marc. Universitat Autònoma de Barcelona; EspañaFil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaSpringer2025-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/277358Corral, Jordi; Rocafort, Mercedes; Ripa, Maria Belén; Vandecaveye, Agustina Irene; Pezzoni, Magdalena; et al.; The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection; Springer; Scientific Reports; 15; 1; 10-2025; 1-142045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-025-19174-5info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-025-19174-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:13:31Zoai:ri.conicet.gov.ar:11336/277358instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:13:31.771CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
title The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
spellingShingle The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
Corral, Jordi
Ralstonia solanacearum
catalases
Reactive oxygen species (ROS)
Environmental stressors
title_short The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
title_full The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
title_fullStr The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
title_full_unstemmed The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
title_sort The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection
dc.creator.none.fl_str_mv Corral, Jordi
Rocafort, Mercedes
Ripa, Maria Belén
Vandecaveye, Agustina Irene
Pezzoni, Magdalena
Invernon, Alicia
Coll, Núria S.
Valls, Marc
Orellano, Elena Graciela
author Corral, Jordi
author_facet Corral, Jordi
Rocafort, Mercedes
Ripa, Maria Belén
Vandecaveye, Agustina Irene
Pezzoni, Magdalena
Invernon, Alicia
Coll, Núria S.
Valls, Marc
Orellano, Elena Graciela
author_role author
author2 Rocafort, Mercedes
Ripa, Maria Belén
Vandecaveye, Agustina Irene
Pezzoni, Magdalena
Invernon, Alicia
Coll, Núria S.
Valls, Marc
Orellano, Elena Graciela
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ralstonia solanacearum
catalases
Reactive oxygen species (ROS)
Environmental stressors
topic Ralstonia solanacearum
catalases
Reactive oxygen species (ROS)
Environmental stressors
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Throughout their life cycle, pathogens are challenged by Reactive Oxygen Species (ROS) and must deploy defence mechanisms against oxidative damage. Environmental stressors such as ultraviolet radiation and desiccation induce intracellular ROS production. Moreover, during pathogen colonisation, plant hosts initiate an oxidative burst, leading to the accumulation of hydrogen peroxide (H2O2). Catalases mitigate ROS toxicity by catalysing the breakdown of H2O2 into water and oxygen. In this study, we explored the roles of the monofunctional catalase (KatE) and the bifunctional catalase-peroxidase (KatG) in the phytopathogen Ralstonia solanacearum during both environmental stress conditions and in planta colonisation stages. Our results demonstrate that both catalases are critical for surviving ROS, with KatG having a more preponderant role. Plant pathogenicity assays show that catalases are not required for virulence although they are important for bacterial fitness in the apoplast. In contrast, we prove that both catalases are vital for R. solanacearum response to oxidative stress in the external environment. KatG was indispensable for survival in soil, as well as for enduring ultraviolet radiation and desiccation exposure. Our findings reveal that the primary function of R. solanacearum catalases is to counteract ROS generated by environmental stressors rather than host-derived oxidative defences. This research identifies specific environmental stressors that these catalases combat during pathogen survival. These insights pave the way for future strategies targeting catalase activity to control bacterial wilt disease.
Fil: Corral, Jordi. Universitat Autònoma de Barcelona; España
Fil: Rocafort, Mercedes. Universitat Autònoma de Barcelona; España
Fil: Ripa, Maria Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Vandecaveye, Agustina Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Pezzoni, Magdalena. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Invernon, Alicia. Universitat Autònoma de Barcelona; España
Fil: Coll, Núria S.. Universitat Autònoma de Barcelona; España
Fil: Valls, Marc. Universitat Autònoma de Barcelona; España
Fil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description Throughout their life cycle, pathogens are challenged by Reactive Oxygen Species (ROS) and must deploy defence mechanisms against oxidative damage. Environmental stressors such as ultraviolet radiation and desiccation induce intracellular ROS production. Moreover, during pathogen colonisation, plant hosts initiate an oxidative burst, leading to the accumulation of hydrogen peroxide (H2O2). Catalases mitigate ROS toxicity by catalysing the breakdown of H2O2 into water and oxygen. In this study, we explored the roles of the monofunctional catalase (KatE) and the bifunctional catalase-peroxidase (KatG) in the phytopathogen Ralstonia solanacearum during both environmental stress conditions and in planta colonisation stages. Our results demonstrate that both catalases are critical for surviving ROS, with KatG having a more preponderant role. Plant pathogenicity assays show that catalases are not required for virulence although they are important for bacterial fitness in the apoplast. In contrast, we prove that both catalases are vital for R. solanacearum response to oxidative stress in the external environment. KatG was indispensable for survival in soil, as well as for enduring ultraviolet radiation and desiccation exposure. Our findings reveal that the primary function of R. solanacearum catalases is to counteract ROS generated by environmental stressors rather than host-derived oxidative defences. This research identifies specific environmental stressors that these catalases combat during pathogen survival. These insights pave the way for future strategies targeting catalase activity to control bacterial wilt disease.
publishDate 2025
dc.date.none.fl_str_mv 2025-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/277358
Corral, Jordi; Rocafort, Mercedes; Ripa, Maria Belén; Vandecaveye, Agustina Irene; Pezzoni, Magdalena; et al.; The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection; Springer; Scientific Reports; 15; 1; 10-2025; 1-14
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/277358
identifier_str_mv Corral, Jordi; Rocafort, Mercedes; Ripa, Maria Belén; Vandecaveye, Agustina Irene; Pezzoni, Magdalena; et al.; The Ralstonia solanacearum catalase-peroxidase KatG is crucial to survive environmental stresses and also plays a role in plant infection; Springer; Scientific Reports; 15; 1; 10-2025; 1-14
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-025-19174-5
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-025-19174-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1852334949197152256
score 12.952241

Publicaciones similares