Genistein effects on Ca 2+ handling in human umbilical artery: Inhibition of sarcoplasmic reticulum Ca 2+ release and of voltage-operated Ca 2+ channels

Autores
Speroni Aguirre, Francisco José; Rebolledo, Alejandro; Salemme, Silvia; Roldán Palomo, R.; Rimorini, Laura; Añon, Maria Cristina; Spinillo, Arsenio; Tanzi, Franco; Milesi, Verónica
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Isoflavones are a group of natural phytoestrogens including the compound genistein. Health beneficial effects have been attributed to the consumption of this compound, but the fact that it has estrogen-like activity has raised doubts regarding its potential risk in infants, newborns, or in the fetus and placenta during pregnancy. This work is aimed at studying genistein effects on Ca 2+ handling by smooth muscle cells of the human umbilical artery (HUA). Using fluorometric techniques, we found that in these cells genistein reduces the intracellular Ca 2+ peak produced by serotonin. The same result could be demonstrated in absence of extracellular Ca 2+, suggesting that the isoflavone reduces Ca 2+ release from the sarcoplasmic reticulum. Force measurement experiments strengthen these results, since genistein reduced the peak force attained by intact HUA rings stimulated by serotonin in a Ca 2+-free solution. Moreover, genistein induced the relaxation of HUA rings precontracted either with serotonin or a depolarizing high-extracellular K + solution, hinting at a reduction of extracellular Ca 2+ entry to the cell. This was confirmed by whole-cell patch-clamp experiments where it was shown that the isoflavone inhibits ionic currents through voltage-operated Ca 2+ channels. In summary, we show that genistein inhibits two mechanisms that could increase intracellular Ca 2+ in human umbilical smooth muscle cells, behaving in this way as a potential vasorelaxing substance of fetal vessels. Taking into account that genistein is able to cross the placental barrier, these data show that isoflavones may have important implications in the regulation of feto-maternal blood flow in pregnant women who consume soy-derived products as part of their meals.
Fil: Speroni Aguirre, Francisco José. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Rebolledo, Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Salemme, Silvia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Roldán Palomo, R.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Rimorini, Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Añon, Maria Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Spinillo, Arsenio. No especifíca;
Fil: Tanzi, Franco. No especifíca;
Fil: Milesi, Verónica. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
CALCIUM CHANNELS
GENISTEIN
HUMAN VASCULAR SMOOTH MUSCLE
UMBILICAL ARTERY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/137962
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Genistein effects on Ca 2+ handling in human umbilical artery: Inhibition of sarcoplasmic reticulum Ca 2+ release and of voltage-operated Ca 2+ channelsSperoni Aguirre, Francisco JoséRebolledo, AlejandroSalemme, SilviaRoldán Palomo, R.Rimorini, LauraAñon, Maria CristinaSpinillo, ArsenioTanzi, FrancoMilesi, VerónicaCALCIUM CHANNELSGENISTEINHUMAN VASCULAR SMOOTH MUSCLEUMBILICAL ARTERYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Isoflavones are a group of natural phytoestrogens including the compound genistein. Health beneficial effects have been attributed to the consumption of this compound, but the fact that it has estrogen-like activity has raised doubts regarding its potential risk in infants, newborns, or in the fetus and placenta during pregnancy. This work is aimed at studying genistein effects on Ca 2+ handling by smooth muscle cells of the human umbilical artery (HUA). Using fluorometric techniques, we found that in these cells genistein reduces the intracellular Ca 2+ peak produced by serotonin. The same result could be demonstrated in absence of extracellular Ca 2+, suggesting that the isoflavone reduces Ca 2+ release from the sarcoplasmic reticulum. Force measurement experiments strengthen these results, since genistein reduced the peak force attained by intact HUA rings stimulated by serotonin in a Ca 2+-free solution. Moreover, genistein induced the relaxation of HUA rings precontracted either with serotonin or a depolarizing high-extracellular K + solution, hinting at a reduction of extracellular Ca 2+ entry to the cell. This was confirmed by whole-cell patch-clamp experiments where it was shown that the isoflavone inhibits ionic currents through voltage-operated Ca 2+ channels. In summary, we show that genistein inhibits two mechanisms that could increase intracellular Ca 2+ in human umbilical smooth muscle cells, behaving in this way as a potential vasorelaxing substance of fetal vessels. Taking into account that genistein is able to cross the placental barrier, these data show that isoflavones may have important implications in the regulation of feto-maternal blood flow in pregnant women who consume soy-derived products as part of their meals.Fil: Speroni Aguirre, Francisco José. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Rebolledo, Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Salemme, Silvia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Roldán Palomo, R.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Rimorini, Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Añon, Maria Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Spinillo, Arsenio. No especifíca;Fil: Tanzi, Franco. No especifíca;Fil: Milesi, Verónica. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaServicio Publicaciones Universidad Navarra2009-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/137962Speroni Aguirre, Francisco José; Rebolledo, Alejandro; Salemme, Silvia; Roldán Palomo, R.; Rimorini, Laura; et al.; Genistein effects on Ca 2+ handling in human umbilical artery: Inhibition of sarcoplasmic reticulum Ca 2+ release and of voltage-operated Ca 2+ channels; Servicio Publicaciones Universidad Navarra; Journal of Physiology and Biochemistry; 65; 2; 6-2009; 113-1241138-75481877-8755CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/BF03179062info:eu-repo/semantics/altIdentifier/url/https://bit.ly/2VmcrNvinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:42Zoai:ri.conicet.gov.ar:11336/137962instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:43.159CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genistein effects on Ca 2+ handling in human umbilical artery: Inhibition of sarcoplasmic reticulum Ca 2+ release and of voltage-operated Ca 2+ channels
title Genistein effects on Ca 2+ handling in human umbilical artery: Inhibition of sarcoplasmic reticulum Ca 2+ release and of voltage-operated Ca 2+ channels
spellingShingle Genistein effects on Ca 2+ handling in human umbilical artery: Inhibition of sarcoplasmic reticulum Ca 2+ release and of voltage-operated Ca 2+ channels
Speroni Aguirre, Francisco José
CALCIUM CHANNELS
GENISTEIN
HUMAN VASCULAR SMOOTH MUSCLE
UMBILICAL ARTERY
title_short Genistein effects on Ca 2+ handling in human umbilical artery: Inhibition of sarcoplasmic reticulum Ca 2+ release and of voltage-operated Ca 2+ channels
title_full Genistein effects on Ca 2+ handling in human umbilical artery: Inhibition of sarcoplasmic reticulum Ca 2+ release and of voltage-operated Ca 2+ channels
title_fullStr Genistein effects on Ca 2+ handling in human umbilical artery: Inhibition of sarcoplasmic reticulum Ca 2+ release and of voltage-operated Ca 2+ channels
title_full_unstemmed Genistein effects on Ca 2+ handling in human umbilical artery: Inhibition of sarcoplasmic reticulum Ca 2+ release and of voltage-operated Ca 2+ channels
title_sort Genistein effects on Ca 2+ handling in human umbilical artery: Inhibition of sarcoplasmic reticulum Ca 2+ release and of voltage-operated Ca 2+ channels
dc.creator.none.fl_str_mv Speroni Aguirre, Francisco José
Rebolledo, Alejandro
Salemme, Silvia
Roldán Palomo, R.
Rimorini, Laura
Añon, Maria Cristina
Spinillo, Arsenio
Tanzi, Franco
Milesi, Verónica
author Speroni Aguirre, Francisco José
author_facet Speroni Aguirre, Francisco José
Rebolledo, Alejandro
Salemme, Silvia
Roldán Palomo, R.
Rimorini, Laura
Añon, Maria Cristina
Spinillo, Arsenio
Tanzi, Franco
Milesi, Verónica
author_role author
author2 Rebolledo, Alejandro
Salemme, Silvia
Roldán Palomo, R.
Rimorini, Laura
Añon, Maria Cristina
Spinillo, Arsenio
Tanzi, Franco
Milesi, Verónica
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CALCIUM CHANNELS
GENISTEIN
HUMAN VASCULAR SMOOTH MUSCLE
UMBILICAL ARTERY
topic CALCIUM CHANNELS
GENISTEIN
HUMAN VASCULAR SMOOTH MUSCLE
UMBILICAL ARTERY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Isoflavones are a group of natural phytoestrogens including the compound genistein. Health beneficial effects have been attributed to the consumption of this compound, but the fact that it has estrogen-like activity has raised doubts regarding its potential risk in infants, newborns, or in the fetus and placenta during pregnancy. This work is aimed at studying genistein effects on Ca 2+ handling by smooth muscle cells of the human umbilical artery (HUA). Using fluorometric techniques, we found that in these cells genistein reduces the intracellular Ca 2+ peak produced by serotonin. The same result could be demonstrated in absence of extracellular Ca 2+, suggesting that the isoflavone reduces Ca 2+ release from the sarcoplasmic reticulum. Force measurement experiments strengthen these results, since genistein reduced the peak force attained by intact HUA rings stimulated by serotonin in a Ca 2+-free solution. Moreover, genistein induced the relaxation of HUA rings precontracted either with serotonin or a depolarizing high-extracellular K + solution, hinting at a reduction of extracellular Ca 2+ entry to the cell. This was confirmed by whole-cell patch-clamp experiments where it was shown that the isoflavone inhibits ionic currents through voltage-operated Ca 2+ channels. In summary, we show that genistein inhibits two mechanisms that could increase intracellular Ca 2+ in human umbilical smooth muscle cells, behaving in this way as a potential vasorelaxing substance of fetal vessels. Taking into account that genistein is able to cross the placental barrier, these data show that isoflavones may have important implications in the regulation of feto-maternal blood flow in pregnant women who consume soy-derived products as part of their meals.
Fil: Speroni Aguirre, Francisco José. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Rebolledo, Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Salemme, Silvia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Roldán Palomo, R.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Rimorini, Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Añon, Maria Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Spinillo, Arsenio. No especifíca;
Fil: Tanzi, Franco. No especifíca;
Fil: Milesi, Verónica. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Isoflavones are a group of natural phytoestrogens including the compound genistein. Health beneficial effects have been attributed to the consumption of this compound, but the fact that it has estrogen-like activity has raised doubts regarding its potential risk in infants, newborns, or in the fetus and placenta during pregnancy. This work is aimed at studying genistein effects on Ca 2+ handling by smooth muscle cells of the human umbilical artery (HUA). Using fluorometric techniques, we found that in these cells genistein reduces the intracellular Ca 2+ peak produced by serotonin. The same result could be demonstrated in absence of extracellular Ca 2+, suggesting that the isoflavone reduces Ca 2+ release from the sarcoplasmic reticulum. Force measurement experiments strengthen these results, since genistein reduced the peak force attained by intact HUA rings stimulated by serotonin in a Ca 2+-free solution. Moreover, genistein induced the relaxation of HUA rings precontracted either with serotonin or a depolarizing high-extracellular K + solution, hinting at a reduction of extracellular Ca 2+ entry to the cell. This was confirmed by whole-cell patch-clamp experiments where it was shown that the isoflavone inhibits ionic currents through voltage-operated Ca 2+ channels. In summary, we show that genistein inhibits two mechanisms that could increase intracellular Ca 2+ in human umbilical smooth muscle cells, behaving in this way as a potential vasorelaxing substance of fetal vessels. Taking into account that genistein is able to cross the placental barrier, these data show that isoflavones may have important implications in the regulation of feto-maternal blood flow in pregnant women who consume soy-derived products as part of their meals.
publishDate 2009
dc.date.none.fl_str_mv 2009-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/137962
Speroni Aguirre, Francisco José; Rebolledo, Alejandro; Salemme, Silvia; Roldán Palomo, R.; Rimorini, Laura; et al.; Genistein effects on Ca 2+ handling in human umbilical artery: Inhibition of sarcoplasmic reticulum Ca 2+ release and of voltage-operated Ca 2+ channels; Servicio Publicaciones Universidad Navarra; Journal of Physiology and Biochemistry; 65; 2; 6-2009; 113-124
1138-7548
1877-8755
CONICET Digital
CONICET
url http://hdl.handle.net/11336/137962
identifier_str_mv Speroni Aguirre, Francisco José; Rebolledo, Alejandro; Salemme, Silvia; Roldán Palomo, R.; Rimorini, Laura; et al.; Genistein effects on Ca 2+ handling in human umbilical artery: Inhibition of sarcoplasmic reticulum Ca 2+ release and of voltage-operated Ca 2+ channels; Servicio Publicaciones Universidad Navarra; Journal of Physiology and Biochemistry; 65; 2; 6-2009; 113-124
1138-7548
1877-8755
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/BF03179062
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
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dc.publisher.none.fl_str_mv Servicio Publicaciones Universidad Navarra
publisher.none.fl_str_mv Servicio Publicaciones Universidad Navarra
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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