In vitro interaction between Bacillus megaterium strains and Caco-2 cells

Autores
López, Ana Claudia; Minnaard, Jessica; Perez, Pablo Fernando; Alippi, Adriana Mónica
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To further our understanding of the virulence potential of Bacillus megaterium strains, cell association and invasion assays were conducted in vitro by infecting human enterocytes (Caco-2 cells) with 53 strains of this bacterium isolated from honey. Two series of experiments were performed: (i) necrosis and cell detachment assays with the supernatants of bacterial culture fi ltrates from 16-h cultures and (ii) adhesion/invasion assays in which cultured enterocytes incubated with bacteria from 3-h cultures were resuspended in Dulbecco’s modifi ed Eagle’s medium and chloramphenicol. The detachment of Caco-2 cells was evaluated by staining the cells with crystal violet. Necrosis was assessed by fl uorescence microscopy of cells labeled with propidium iodide. Association (adhesion plus invasion) was determined by plate counts and invasion in an aminoglycoside protection assay. The results showed that spent culture supernatants detached and necrotized Caco-2 cells in a strain-dependent manner. Seven out of 53 B. megaterium fi ltered culture supernatants caused complete cell detachment. Suspensions of these same bacterial strains adhered and invaded enterocytes in 2-h infection experiments. To our knowledge, this is the fi rst report on the interaction between B. megaterium and intestinal epithelial Caco-2 cells.
Fil: López, Ana Claudia. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro en Investigación de Fitopatología; Argentina
Fil: Minnaard, Jessica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina
Fil: Perez, Pablo Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
Fil: Alippi, Adriana Mónica. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro en Investigación de Fitopatología; Argentina
Materia
Bacillus Megaterium
Caco-2 Cells
Adhesion
Cellular Necrosis
Spent Culture Supernatant
Honey
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2172
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/2172
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro interaction between Bacillus megaterium strains and Caco-2 cellsLópez, Ana ClaudiaMinnaard, JessicaPerez, Pablo FernandoAlippi, Adriana MónicaBacillus MegateriumCaco-2 CellsAdhesionCellular NecrosisSpent Culture SupernatantHoneyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1To further our understanding of the virulence potential of Bacillus megaterium strains, cell association and invasion assays were conducted in vitro by infecting human enterocytes (Caco-2 cells) with 53 strains of this bacterium isolated from honey. Two series of experiments were performed: (i) necrosis and cell detachment assays with the supernatants of bacterial culture fi ltrates from 16-h cultures and (ii) adhesion/invasion assays in which cultured enterocytes incubated with bacteria from 3-h cultures were resuspended in Dulbecco’s modifi ed Eagle’s medium and chloramphenicol. The detachment of Caco-2 cells was evaluated by staining the cells with crystal violet. Necrosis was assessed by fl uorescence microscopy of cells labeled with propidium iodide. Association (adhesion plus invasion) was determined by plate counts and invasion in an aminoglycoside protection assay. The results showed that spent culture supernatants detached and necrotized Caco-2 cells in a strain-dependent manner. Seven out of 53 B. megaterium fi ltered culture supernatants caused complete cell detachment. Suspensions of these same bacterial strains adhered and invaded enterocytes in 2-h infection experiments. To our knowledge, this is the fi rst report on the interaction between B. megaterium and intestinal epithelial Caco-2 cells.Fil: López, Ana Claudia. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro en Investigación de Fitopatología; ArgentinaFil: Minnaard, Jessica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); ArgentinaFil: Perez, Pablo Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; ArgentinaFil: Alippi, Adriana Mónica. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro en Investigación de Fitopatología; ArgentinaSpanish Society for Microbiology2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2172López, Ana Claudia; Minnaard, Jessica; Perez, Pablo Fernando; Alippi, Adriana Mónica; In vitro interaction between Bacillus megaterium strains and Caco-2 cells; Spanish Society for Microbiology; International Microbiology; 16; 1; 3-2013; 27-331139-67091618-1905enginfo:eu-repo/semantics/altIdentifier/url/http://revistes.iec.cat/index.php/IM/article/view/67274info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:38:26Zoai:ri.conicet.gov.ar:11336/2172instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:38:26.374CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro interaction between Bacillus megaterium strains and Caco-2 cells
title In vitro interaction between Bacillus megaterium strains and Caco-2 cells
spellingShingle In vitro interaction between Bacillus megaterium strains and Caco-2 cells
López, Ana Claudia
Bacillus Megaterium
Caco-2 Cells
Adhesion
Cellular Necrosis
Spent Culture Supernatant
Honey
title_short In vitro interaction between Bacillus megaterium strains and Caco-2 cells
title_full In vitro interaction between Bacillus megaterium strains and Caco-2 cells
title_fullStr In vitro interaction between Bacillus megaterium strains and Caco-2 cells
title_full_unstemmed In vitro interaction between Bacillus megaterium strains and Caco-2 cells
title_sort In vitro interaction between Bacillus megaterium strains and Caco-2 cells
dc.creator.none.fl_str_mv López, Ana Claudia
Minnaard, Jessica
Perez, Pablo Fernando
Alippi, Adriana Mónica
author López, Ana Claudia
author_facet López, Ana Claudia
Minnaard, Jessica
Perez, Pablo Fernando
Alippi, Adriana Mónica
author_role author
author2 Minnaard, Jessica
Perez, Pablo Fernando
Alippi, Adriana Mónica
author2_role author
author
author
dc.subject.none.fl_str_mv Bacillus Megaterium
Caco-2 Cells
Adhesion
Cellular Necrosis
Spent Culture Supernatant
Honey
topic Bacillus Megaterium
Caco-2 Cells
Adhesion
Cellular Necrosis
Spent Culture Supernatant
Honey
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To further our understanding of the virulence potential of Bacillus megaterium strains, cell association and invasion assays were conducted in vitro by infecting human enterocytes (Caco-2 cells) with 53 strains of this bacterium isolated from honey. Two series of experiments were performed: (i) necrosis and cell detachment assays with the supernatants of bacterial culture fi ltrates from 16-h cultures and (ii) adhesion/invasion assays in which cultured enterocytes incubated with bacteria from 3-h cultures were resuspended in Dulbecco’s modifi ed Eagle’s medium and chloramphenicol. The detachment of Caco-2 cells was evaluated by staining the cells with crystal violet. Necrosis was assessed by fl uorescence microscopy of cells labeled with propidium iodide. Association (adhesion plus invasion) was determined by plate counts and invasion in an aminoglycoside protection assay. The results showed that spent culture supernatants detached and necrotized Caco-2 cells in a strain-dependent manner. Seven out of 53 B. megaterium fi ltered culture supernatants caused complete cell detachment. Suspensions of these same bacterial strains adhered and invaded enterocytes in 2-h infection experiments. To our knowledge, this is the fi rst report on the interaction between B. megaterium and intestinal epithelial Caco-2 cells.
Fil: López, Ana Claudia. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro en Investigación de Fitopatología; Argentina
Fil: Minnaard, Jessica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina
Fil: Perez, Pablo Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
Fil: Alippi, Adriana Mónica. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro en Investigación de Fitopatología; Argentina
description To further our understanding of the virulence potential of Bacillus megaterium strains, cell association and invasion assays were conducted in vitro by infecting human enterocytes (Caco-2 cells) with 53 strains of this bacterium isolated from honey. Two series of experiments were performed: (i) necrosis and cell detachment assays with the supernatants of bacterial culture fi ltrates from 16-h cultures and (ii) adhesion/invasion assays in which cultured enterocytes incubated with bacteria from 3-h cultures were resuspended in Dulbecco’s modifi ed Eagle’s medium and chloramphenicol. The detachment of Caco-2 cells was evaluated by staining the cells with crystal violet. Necrosis was assessed by fl uorescence microscopy of cells labeled with propidium iodide. Association (adhesion plus invasion) was determined by plate counts and invasion in an aminoglycoside protection assay. The results showed that spent culture supernatants detached and necrotized Caco-2 cells in a strain-dependent manner. Seven out of 53 B. megaterium fi ltered culture supernatants caused complete cell detachment. Suspensions of these same bacterial strains adhered and invaded enterocytes in 2-h infection experiments. To our knowledge, this is the fi rst report on the interaction between B. megaterium and intestinal epithelial Caco-2 cells.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2172
López, Ana Claudia; Minnaard, Jessica; Perez, Pablo Fernando; Alippi, Adriana Mónica; In vitro interaction between Bacillus megaterium strains and Caco-2 cells; Spanish Society for Microbiology; International Microbiology; 16; 1; 3-2013; 27-33
1139-6709
1618-1905
url http://hdl.handle.net/11336/2172
identifier_str_mv López, Ana Claudia; Minnaard, Jessica; Perez, Pablo Fernando; Alippi, Adriana Mónica; In vitro interaction between Bacillus megaterium strains and Caco-2 cells; Spanish Society for Microbiology; International Microbiology; 16; 1; 3-2013; 27-33
1139-6709
1618-1905
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://revistes.iec.cat/index.php/IM/article/view/67274
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Spanish Society for Microbiology
publisher.none.fl_str_mv Spanish Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782041104842752
score 12.982451

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