IFN-γ plays a detrimental role in murine defense against nasal colonization of Staphylococcus aureus

Autores
Satorres, Sara Elena; Alcaráz, Lucía Esther; Cargnelutti, Ethelina; Di Genaro, Maria Silvia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The anterior nares are the major reservoir in humans of Staphylococcus aureus with the risk of developing endogenous infections or transmitting infections to susceptible persons. The mechanisms that mediate attachment of staphylococci to the nasal mucosa are little known. The purpose of the present work was to study some factors that could influence the nasal colonization in an animal model of mice. We investigated the possible role of IFN-γ. We used S. aureus ATCC 35556 (SA113) slime-producing and ATCC 25923 non-slime-producing strains. Male 6-week-old BALB/c, C57BL/6 (wild-type, WT), and gene-deficient IL-12p40 (IL-12p40-/-) or IL-4 (IL-4-/-) mice on C57BL/6 background were infected with a dose of S. aureus of 106 CFU in 10 μl of saline. The total number of S. aureus CFU per nose and lung, specific IgA response and IFN-γ levels were evaluated. Significant higher CFU were recovery from the narines of C57BL/6 compared with BALB/c mice either after ATCC 35556 (p < 0.0001) and ATCC 25923 (p < 0.02) strain infection. Low IgA response correlated with high bacterial counting in the C57BL/6 nasal region. Moreover, C57BL/6 mice showed major colonization of slime-producing S. aureus ATCC 35556 than non-slime-producing ATCC 25923 S. aureus strain (p < 0.02). IL-12p40-/-mice clarified the bacteria from their nose more efficiently that WT mice after slime-producing S. aureus (p < 0.0001). Accordingly, significant lower level of IFN-γ were detected in IL-12p40-/- compared with WT mice after infection with this strain (p < 0.03). The results suggested the influence of the slime production in nasal colonization of S. aureus, and indicate at first time that IFN-γ may play a detrimental role in this mucosal infection. These results could contribute to elucidate mucosal immune mechanisms involved in S. aureus colonization and then control infections in susceptible persons.
Fil: Satorres, Sara Elena. Universidad Nacional de San Luis; Argentina
Fil: Alcaráz, Lucía Esther. Universidad Nacional de San Luis; Argentina
Fil: Cargnelutti, Ethelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Materia
IFN-Γ
NASAL COLONIZATION
SLIME PRODUCTION
STAPHYLOCOCCUS AUREUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/130346
Acceder
id CONICETDig_58ec3aaf870ad989840b187a2b2ecc15
oai_identifier_str oai:ri.conicet.gov.ar:11336/130346
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling IFN-γ plays a detrimental role in murine defense against nasal colonization of Staphylococcus aureusSatorres, Sara ElenaAlcaráz, Lucía EstherCargnelutti, EthelinaDi Genaro, Maria SilviaIFN-ΓNASAL COLONIZATIONSLIME PRODUCTIONSTAPHYLOCOCCUS AUREUShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The anterior nares are the major reservoir in humans of Staphylococcus aureus with the risk of developing endogenous infections or transmitting infections to susceptible persons. The mechanisms that mediate attachment of staphylococci to the nasal mucosa are little known. The purpose of the present work was to study some factors that could influence the nasal colonization in an animal model of mice. We investigated the possible role of IFN-γ. We used S. aureus ATCC 35556 (SA113) slime-producing and ATCC 25923 non-slime-producing strains. Male 6-week-old BALB/c, C57BL/6 (wild-type, WT), and gene-deficient IL-12p40 (IL-12p40-/-) or IL-4 (IL-4-/-) mice on C57BL/6 background were infected with a dose of S. aureus of 106 CFU in 10 μl of saline. The total number of S. aureus CFU per nose and lung, specific IgA response and IFN-γ levels were evaluated. Significant higher CFU were recovery from the narines of C57BL/6 compared with BALB/c mice either after ATCC 35556 (p < 0.0001) and ATCC 25923 (p < 0.02) strain infection. Low IgA response correlated with high bacterial counting in the C57BL/6 nasal region. Moreover, C57BL/6 mice showed major colonization of slime-producing S. aureus ATCC 35556 than non-slime-producing ATCC 25923 S. aureus strain (p < 0.02). IL-12p40-/-mice clarified the bacteria from their nose more efficiently that WT mice after slime-producing S. aureus (p < 0.0001). Accordingly, significant lower level of IFN-γ were detected in IL-12p40-/- compared with WT mice after infection with this strain (p < 0.03). The results suggested the influence of the slime production in nasal colonization of S. aureus, and indicate at first time that IFN-γ may play a detrimental role in this mucosal infection. These results could contribute to elucidate mucosal immune mechanisms involved in S. aureus colonization and then control infections in susceptible persons.Fil: Satorres, Sara Elena. Universidad Nacional de San Luis; ArgentinaFil: Alcaráz, Lucía Esther. Universidad Nacional de San Luis; ArgentinaFil: Cargnelutti, Ethelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaElsevier Science2009-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/130346Satorres, Sara Elena; Alcaráz, Lucía Esther; Cargnelutti, Ethelina; Di Genaro, Maria Silvia; IFN-γ plays a detrimental role in murine defense against nasal colonization of Staphylococcus aureus; Elsevier Science; Immunology Letters; 123; 2; 3-2009; 185-1880165-2478CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165247809000637info:eu-repo/semantics/altIdentifier/doi/10.1016/j.imlet.2009.03.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:28Zoai:ri.conicet.gov.ar:11336/130346instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:29.082CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv IFN-γ plays a detrimental role in murine defense against nasal colonization of Staphylococcus aureus
title IFN-γ plays a detrimental role in murine defense against nasal colonization of Staphylococcus aureus
spellingShingle IFN-γ plays a detrimental role in murine defense against nasal colonization of Staphylococcus aureus
Satorres, Sara Elena
IFN-Γ
NASAL COLONIZATION
SLIME PRODUCTION
STAPHYLOCOCCUS AUREUS
title_short IFN-γ plays a detrimental role in murine defense against nasal colonization of Staphylococcus aureus
title_full IFN-γ plays a detrimental role in murine defense against nasal colonization of Staphylococcus aureus
title_fullStr IFN-γ plays a detrimental role in murine defense against nasal colonization of Staphylococcus aureus
title_full_unstemmed IFN-γ plays a detrimental role in murine defense against nasal colonization of Staphylococcus aureus
title_sort IFN-γ plays a detrimental role in murine defense against nasal colonization of Staphylococcus aureus
dc.creator.none.fl_str_mv Satorres, Sara Elena
Alcaráz, Lucía Esther
Cargnelutti, Ethelina
Di Genaro, Maria Silvia
author Satorres, Sara Elena
author_facet Satorres, Sara Elena
Alcaráz, Lucía Esther
Cargnelutti, Ethelina
Di Genaro, Maria Silvia
author_role author
author2 Alcaráz, Lucía Esther
Cargnelutti, Ethelina
Di Genaro, Maria Silvia
author2_role author
author
author
dc.subject.none.fl_str_mv IFN-Γ
NASAL COLONIZATION
SLIME PRODUCTION
STAPHYLOCOCCUS AUREUS
topic IFN-Γ
NASAL COLONIZATION
SLIME PRODUCTION
STAPHYLOCOCCUS AUREUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The anterior nares are the major reservoir in humans of Staphylococcus aureus with the risk of developing endogenous infections or transmitting infections to susceptible persons. The mechanisms that mediate attachment of staphylococci to the nasal mucosa are little known. The purpose of the present work was to study some factors that could influence the nasal colonization in an animal model of mice. We investigated the possible role of IFN-γ. We used S. aureus ATCC 35556 (SA113) slime-producing and ATCC 25923 non-slime-producing strains. Male 6-week-old BALB/c, C57BL/6 (wild-type, WT), and gene-deficient IL-12p40 (IL-12p40-/-) or IL-4 (IL-4-/-) mice on C57BL/6 background were infected with a dose of S. aureus of 106 CFU in 10 μl of saline. The total number of S. aureus CFU per nose and lung, specific IgA response and IFN-γ levels were evaluated. Significant higher CFU were recovery from the narines of C57BL/6 compared with BALB/c mice either after ATCC 35556 (p < 0.0001) and ATCC 25923 (p < 0.02) strain infection. Low IgA response correlated with high bacterial counting in the C57BL/6 nasal region. Moreover, C57BL/6 mice showed major colonization of slime-producing S. aureus ATCC 35556 than non-slime-producing ATCC 25923 S. aureus strain (p < 0.02). IL-12p40-/-mice clarified the bacteria from their nose more efficiently that WT mice after slime-producing S. aureus (p < 0.0001). Accordingly, significant lower level of IFN-γ were detected in IL-12p40-/- compared with WT mice after infection with this strain (p < 0.03). The results suggested the influence of the slime production in nasal colonization of S. aureus, and indicate at first time that IFN-γ may play a detrimental role in this mucosal infection. These results could contribute to elucidate mucosal immune mechanisms involved in S. aureus colonization and then control infections in susceptible persons.
Fil: Satorres, Sara Elena. Universidad Nacional de San Luis; Argentina
Fil: Alcaráz, Lucía Esther. Universidad Nacional de San Luis; Argentina
Fil: Cargnelutti, Ethelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
description The anterior nares are the major reservoir in humans of Staphylococcus aureus with the risk of developing endogenous infections or transmitting infections to susceptible persons. The mechanisms that mediate attachment of staphylococci to the nasal mucosa are little known. The purpose of the present work was to study some factors that could influence the nasal colonization in an animal model of mice. We investigated the possible role of IFN-γ. We used S. aureus ATCC 35556 (SA113) slime-producing and ATCC 25923 non-slime-producing strains. Male 6-week-old BALB/c, C57BL/6 (wild-type, WT), and gene-deficient IL-12p40 (IL-12p40-/-) or IL-4 (IL-4-/-) mice on C57BL/6 background were infected with a dose of S. aureus of 106 CFU in 10 μl of saline. The total number of S. aureus CFU per nose and lung, specific IgA response and IFN-γ levels were evaluated. Significant higher CFU were recovery from the narines of C57BL/6 compared with BALB/c mice either after ATCC 35556 (p < 0.0001) and ATCC 25923 (p < 0.02) strain infection. Low IgA response correlated with high bacterial counting in the C57BL/6 nasal region. Moreover, C57BL/6 mice showed major colonization of slime-producing S. aureus ATCC 35556 than non-slime-producing ATCC 25923 S. aureus strain (p < 0.02). IL-12p40-/-mice clarified the bacteria from their nose more efficiently that WT mice after slime-producing S. aureus (p < 0.0001). Accordingly, significant lower level of IFN-γ were detected in IL-12p40-/- compared with WT mice after infection with this strain (p < 0.03). The results suggested the influence of the slime production in nasal colonization of S. aureus, and indicate at first time that IFN-γ may play a detrimental role in this mucosal infection. These results could contribute to elucidate mucosal immune mechanisms involved in S. aureus colonization and then control infections in susceptible persons.
publishDate 2009
dc.date.none.fl_str_mv 2009-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/130346
Satorres, Sara Elena; Alcaráz, Lucía Esther; Cargnelutti, Ethelina; Di Genaro, Maria Silvia; IFN-γ plays a detrimental role in murine defense against nasal colonization of Staphylococcus aureus; Elsevier Science; Immunology Letters; 123; 2; 3-2009; 185-188
0165-2478
CONICET Digital
CONICET
url http://hdl.handle.net/11336/130346
identifier_str_mv Satorres, Sara Elena; Alcaráz, Lucía Esther; Cargnelutti, Ethelina; Di Genaro, Maria Silvia; IFN-γ plays a detrimental role in murine defense against nasal colonization of Staphylococcus aureus; Elsevier Science; Immunology Letters; 123; 2; 3-2009; 185-188
0165-2478
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0165247809000637
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.imlet.2009.03.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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