Immunogenicity of HLA Class i and II double restricted influenza a-derived peptides

Autores
Pedersen, Sara Ram; Christensen, Jan Pravsgaard; Buus, Søren; Rasmussen, Michael; Korsholm, Karen Smith; Nielsen, Morten; Claesson, Mogens Helweg
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to identify influenza A-derived peptides which bind to both HLA class I and-II molecules and by immunization lead to both HLA class I and class II restricted immune responses. Eight influenza A-derived 9-11mer peptides with simultaneous binding to both HLA-A02:01 and HLA-DRB101:01 molecules were identified by bioinformatics and biochemical technology. Immunization of transgenic HLA-A02:01/HLADRB101:01 mice with four of these double binding peptides gave rise to both HLA class I and class II restricted responses by CD8 and CD4 T cells, respectively, whereas four of the double binding peptides did result in HLA-A02:01 restricted responses only. According to their cytokine profile, the CD4 T cell responses were of the Th2 type. In influenza infected mice, we were unable to detect natural processing in vivo of the double restricted peptides and in line with this, peptide vaccination did not decrease virus titres in the lungs of intranasally influenza challenged mice. Our data show that HLA class I and class II double binding peptides can be identified by bioinformatics and biochemical technology. By immunization, double binding peptides can give rise to both HLA class I and class I restricted responses, a quality which might be of potential interest for peptide-based vaccine development.
Fil: Pedersen, Sara Ram. Universidad de Copenhagen; Dinamarca
Fil: Christensen, Jan Pravsgaard. Universidad de Copenhagen; Dinamarca
Fil: Buus, Søren. Universidad de Copenhagen; Dinamarca
Fil: Rasmussen, Michael. Universidad de Copenhagen; Dinamarca
Fil: Korsholm, Karen Smith. Statens Serum Institute; Dinamarca
Fil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Claesson, Mogens Helweg. Universidad de Copenhagen; Dinamarca
Materia
Double Restricted Influenza A-Derived Peptides
Immunoinformatics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49404
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/49404
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunogenicity of HLA Class i and II double restricted influenza a-derived peptidesPedersen, Sara RamChristensen, Jan PravsgaardBuus, SørenRasmussen, MichaelKorsholm, Karen SmithNielsen, MortenClaesson, Mogens HelwegDouble Restricted Influenza A-Derived PeptidesImmunoinformaticshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The aim of the present study was to identify influenza A-derived peptides which bind to both HLA class I and-II molecules and by immunization lead to both HLA class I and class II restricted immune responses. Eight influenza A-derived 9-11mer peptides with simultaneous binding to both HLA-A02:01 and HLA-DRB101:01 molecules were identified by bioinformatics and biochemical technology. Immunization of transgenic HLA-A02:01/HLADRB101:01 mice with four of these double binding peptides gave rise to both HLA class I and class II restricted responses by CD8 and CD4 T cells, respectively, whereas four of the double binding peptides did result in HLA-A02:01 restricted responses only. According to their cytokine profile, the CD4 T cell responses were of the Th2 type. In influenza infected mice, we were unable to detect natural processing in vivo of the double restricted peptides and in line with this, peptide vaccination did not decrease virus titres in the lungs of intranasally influenza challenged mice. Our data show that HLA class I and class II double binding peptides can be identified by bioinformatics and biochemical technology. By immunization, double binding peptides can give rise to both HLA class I and class I restricted responses, a quality which might be of potential interest for peptide-based vaccine development.Fil: Pedersen, Sara Ram. Universidad de Copenhagen; DinamarcaFil: Christensen, Jan Pravsgaard. Universidad de Copenhagen; DinamarcaFil: Buus, Søren. Universidad de Copenhagen; DinamarcaFil: Rasmussen, Michael. Universidad de Copenhagen; DinamarcaFil: Korsholm, Karen Smith. Statens Serum Institute; DinamarcaFil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Claesson, Mogens Helweg. Universidad de Copenhagen; DinamarcaPublic Library of Science2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49404Pedersen, Sara Ram; Christensen, Jan Pravsgaard; Buus, Søren; Rasmussen, Michael; Korsholm, Karen Smith; et al.; Immunogenicity of HLA Class i and II double restricted influenza a-derived peptides; Public Library of Science; Plos One; 11; 1; 1-2016; 1-16; e0145621932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0145629info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0145629info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-08T12:47:48Zoai:ri.conicet.gov.ar:11336/49404instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-08 12:47:48.391CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunogenicity of HLA Class i and II double restricted influenza a-derived peptides
title Immunogenicity of HLA Class i and II double restricted influenza a-derived peptides
spellingShingle Immunogenicity of HLA Class i and II double restricted influenza a-derived peptides
Pedersen, Sara Ram
Double Restricted Influenza A-Derived Peptides
Immunoinformatics
title_short Immunogenicity of HLA Class i and II double restricted influenza a-derived peptides
title_full Immunogenicity of HLA Class i and II double restricted influenza a-derived peptides
title_fullStr Immunogenicity of HLA Class i and II double restricted influenza a-derived peptides
title_full_unstemmed Immunogenicity of HLA Class i and II double restricted influenza a-derived peptides
title_sort Immunogenicity of HLA Class i and II double restricted influenza a-derived peptides
dc.creator.none.fl_str_mv Pedersen, Sara Ram
Christensen, Jan Pravsgaard
Buus, Søren
Rasmussen, Michael
Korsholm, Karen Smith
Nielsen, Morten
Claesson, Mogens Helweg
author Pedersen, Sara Ram
author_facet Pedersen, Sara Ram
Christensen, Jan Pravsgaard
Buus, Søren
Rasmussen, Michael
Korsholm, Karen Smith
Nielsen, Morten
Claesson, Mogens Helweg
author_role author
author2 Christensen, Jan Pravsgaard
Buus, Søren
Rasmussen, Michael
Korsholm, Karen Smith
Nielsen, Morten
Claesson, Mogens Helweg
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Double Restricted Influenza A-Derived Peptides
Immunoinformatics
topic Double Restricted Influenza A-Derived Peptides
Immunoinformatics
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of the present study was to identify influenza A-derived peptides which bind to both HLA class I and-II molecules and by immunization lead to both HLA class I and class II restricted immune responses. Eight influenza A-derived 9-11mer peptides with simultaneous binding to both HLA-A02:01 and HLA-DRB101:01 molecules were identified by bioinformatics and biochemical technology. Immunization of transgenic HLA-A02:01/HLADRB101:01 mice with four of these double binding peptides gave rise to both HLA class I and class II restricted responses by CD8 and CD4 T cells, respectively, whereas four of the double binding peptides did result in HLA-A02:01 restricted responses only. According to their cytokine profile, the CD4 T cell responses were of the Th2 type. In influenza infected mice, we were unable to detect natural processing in vivo of the double restricted peptides and in line with this, peptide vaccination did not decrease virus titres in the lungs of intranasally influenza challenged mice. Our data show that HLA class I and class II double binding peptides can be identified by bioinformatics and biochemical technology. By immunization, double binding peptides can give rise to both HLA class I and class I restricted responses, a quality which might be of potential interest for peptide-based vaccine development.
Fil: Pedersen, Sara Ram. Universidad de Copenhagen; Dinamarca
Fil: Christensen, Jan Pravsgaard. Universidad de Copenhagen; Dinamarca
Fil: Buus, Søren. Universidad de Copenhagen; Dinamarca
Fil: Rasmussen, Michael. Universidad de Copenhagen; Dinamarca
Fil: Korsholm, Karen Smith. Statens Serum Institute; Dinamarca
Fil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Claesson, Mogens Helweg. Universidad de Copenhagen; Dinamarca
description The aim of the present study was to identify influenza A-derived peptides which bind to both HLA class I and-II molecules and by immunization lead to both HLA class I and class II restricted immune responses. Eight influenza A-derived 9-11mer peptides with simultaneous binding to both HLA-A02:01 and HLA-DRB101:01 molecules were identified by bioinformatics and biochemical technology. Immunization of transgenic HLA-A02:01/HLADRB101:01 mice with four of these double binding peptides gave rise to both HLA class I and class II restricted responses by CD8 and CD4 T cells, respectively, whereas four of the double binding peptides did result in HLA-A02:01 restricted responses only. According to their cytokine profile, the CD4 T cell responses were of the Th2 type. In influenza infected mice, we were unable to detect natural processing in vivo of the double restricted peptides and in line with this, peptide vaccination did not decrease virus titres in the lungs of intranasally influenza challenged mice. Our data show that HLA class I and class II double binding peptides can be identified by bioinformatics and biochemical technology. By immunization, double binding peptides can give rise to both HLA class I and class I restricted responses, a quality which might be of potential interest for peptide-based vaccine development.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49404
Pedersen, Sara Ram; Christensen, Jan Pravsgaard; Buus, Søren; Rasmussen, Michael; Korsholm, Karen Smith; et al.; Immunogenicity of HLA Class i and II double restricted influenza a-derived peptides; Public Library of Science; Plos One; 11; 1; 1-2016; 1-16; e014562
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49404
identifier_str_mv Pedersen, Sara Ram; Christensen, Jan Pravsgaard; Buus, Søren; Rasmussen, Michael; Korsholm, Karen Smith; et al.; Immunogenicity of HLA Class i and II double restricted influenza a-derived peptides; Public Library of Science; Plos One; 11; 1; 1-2016; 1-16; e014562
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0145629
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0145629
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.25844

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