CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy

Autores
Yokobori, Noemí; Schierloh, Luis Pablo; Geffner, Laura Judith; Balboa, Luciana; Romero, María Mercedes; Musella, Rosa María; Castagnino, J.; De Stéfano, G.; Alemán, Mercedes; de la Barrera, Silvia Susana; Abbate, E.; Sasiain, María del Carmen
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Summary Tuberculous pleurisy is a naturally occurring site of Mycobacterium tuberculosis (Mtb) infection. Herein, we describe the expression of activation, natural killer (NK) and cell migration markers, as well as effector functions from γδT cells in peripheral blood (PB) and pleural effusion (PE) from tuberculosis patients (TB). We observed a decreased percentage of circulating γδT from TB patients and differential expression of NK as well as of chemokine receptors on PB and PE. Two subsets of γδT cells were differentiated by the CD3/γδT cell receptor (γδTCR) complex. The γδTCRlow subset had a higher CD3 to TCR ratio and was enriched in Vδ2+ cells, whereas most Vδ1+ cells belonged to the γδTCR high subset. In PB from TB, most γδTCRhigh were CD45RA+CCR7- and γδTCRlow were CD45RA+/-CCR7+CXCR3+. In the pleural space the proportion of CD45RA-CCR7+CXCR3+ cells was higher. Neither spontaneous nor Mtb-induced interferon (IFN)-γ production was observed in PB-γδT cells from TB; however, PE-γδT cells showed a strong response. Both PB- and PE-γδ T cells expressed surface CD107a upon stimulation with Mtb. Notably, PE-γδTCR low cells were the most potent effector cells. Thus, γδT cells from PB would acquire a further activated phenotype within the site of Mtb infection and exert full effector functions. As γδT cells produce IFN-γ within the pleural space, they would be expected to play a beneficial role in tuberculous pleurisy by helping to maintain a T helper type 1 profile. © 2009 British Society for Immunology.
Fil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Schierloh, Luis Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Geffner, Laura Judith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Balboa, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Romero, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Musella, Rosa María. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Castagnino, J.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: De Stéfano, G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Alemán, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: de la Barrera, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Abbate, E.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Materia
ΓΔ T CELL
CD107A
IFN-Γ
MEMORY CELL
TUBERCULOUS PLEURISY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54878
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54878
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisyYokobori, NoemíSchierloh, Luis PabloGeffner, Laura JudithBalboa, LucianaRomero, María MercedesMusella, Rosa MaríaCastagnino, J.De Stéfano, G.Alemán, Mercedesde la Barrera, Silvia SusanaAbbate, E.Sasiain, María del CarmenΓΔ T CELLCD107AIFN-ΓMEMORY CELLTUBERCULOUS PLEURISYhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Summary Tuberculous pleurisy is a naturally occurring site of Mycobacterium tuberculosis (Mtb) infection. Herein, we describe the expression of activation, natural killer (NK) and cell migration markers, as well as effector functions from γδT cells in peripheral blood (PB) and pleural effusion (PE) from tuberculosis patients (TB). We observed a decreased percentage of circulating γδT from TB patients and differential expression of NK as well as of chemokine receptors on PB and PE. Two subsets of γδT cells were differentiated by the CD3/γδT cell receptor (γδTCR) complex. The γδTCRlow subset had a higher CD3 to TCR ratio and was enriched in Vδ2+ cells, whereas most Vδ1+ cells belonged to the γδTCR high subset. In PB from TB, most γδTCRhigh were CD45RA+CCR7- and γδTCRlow were CD45RA+/-CCR7+CXCR3+. In the pleural space the proportion of CD45RA-CCR7+CXCR3+ cells was higher. Neither spontaneous nor Mtb-induced interferon (IFN)-γ production was observed in PB-γδT cells from TB; however, PE-γδT cells showed a strong response. Both PB- and PE-γδ T cells expressed surface CD107a upon stimulation with Mtb. Notably, PE-γδTCR low cells were the most potent effector cells. Thus, γδT cells from PB would acquire a further activated phenotype within the site of Mtb infection and exert full effector functions. As γδT cells produce IFN-γ within the pleural space, they would be expected to play a beneficial role in tuberculous pleurisy by helping to maintain a T helper type 1 profile. © 2009 British Society for Immunology.Fil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; ArgentinaFil: Schierloh, Luis Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; ArgentinaFil: Geffner, Laura Judith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; ArgentinaFil: Balboa, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; ArgentinaFil: Romero, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; ArgentinaFil: Musella, Rosa María. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Castagnino, J.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: De Stéfano, G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Alemán, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; ArgentinaFil: de la Barrera, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; ArgentinaFil: Abbate, E.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; ArgentinaWiley Blackwell Publishing, Inc2009-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54878Yokobori, Noemí; Schierloh, Luis Pablo; Geffner, Laura Judith; Balboa, Luciana; Romero, María Mercedes; et al.; CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy; Wiley Blackwell Publishing, Inc; Clinical and Experimental Immunology; 157; 3; 9-2009; 385-3940009-9104CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2249.2009.03974.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2249.2009.03974.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:46Zoai:ri.conicet.gov.ar:11336/54878instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:46.372CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy
title CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy
spellingShingle CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy
Yokobori, Noemí
ΓΔ T CELL
CD107A
IFN-Γ
MEMORY CELL
TUBERCULOUS PLEURISY
title_short CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy
title_full CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy
title_fullStr CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy
title_full_unstemmed CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy
title_sort CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy
dc.creator.none.fl_str_mv Yokobori, Noemí
Schierloh, Luis Pablo
Geffner, Laura Judith
Balboa, Luciana
Romero, María Mercedes
Musella, Rosa María
Castagnino, J.
De Stéfano, G.
Alemán, Mercedes
de la Barrera, Silvia Susana
Abbate, E.
Sasiain, María del Carmen
author Yokobori, Noemí
author_facet Yokobori, Noemí
Schierloh, Luis Pablo
Geffner, Laura Judith
Balboa, Luciana
Romero, María Mercedes
Musella, Rosa María
Castagnino, J.
De Stéfano, G.
Alemán, Mercedes
de la Barrera, Silvia Susana
Abbate, E.
Sasiain, María del Carmen
author_role author
author2 Schierloh, Luis Pablo
Geffner, Laura Judith
Balboa, Luciana
Romero, María Mercedes
Musella, Rosa María
Castagnino, J.
De Stéfano, G.
Alemán, Mercedes
de la Barrera, Silvia Susana
Abbate, E.
Sasiain, María del Carmen
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ΓΔ T CELL
CD107A
IFN-Γ
MEMORY CELL
TUBERCULOUS PLEURISY
topic ΓΔ T CELL
CD107A
IFN-Γ
MEMORY CELL
TUBERCULOUS PLEURISY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Summary Tuberculous pleurisy is a naturally occurring site of Mycobacterium tuberculosis (Mtb) infection. Herein, we describe the expression of activation, natural killer (NK) and cell migration markers, as well as effector functions from γδT cells in peripheral blood (PB) and pleural effusion (PE) from tuberculosis patients (TB). We observed a decreased percentage of circulating γδT from TB patients and differential expression of NK as well as of chemokine receptors on PB and PE. Two subsets of γδT cells were differentiated by the CD3/γδT cell receptor (γδTCR) complex. The γδTCRlow subset had a higher CD3 to TCR ratio and was enriched in Vδ2+ cells, whereas most Vδ1+ cells belonged to the γδTCR high subset. In PB from TB, most γδTCRhigh were CD45RA+CCR7- and γδTCRlow were CD45RA+/-CCR7+CXCR3+. In the pleural space the proportion of CD45RA-CCR7+CXCR3+ cells was higher. Neither spontaneous nor Mtb-induced interferon (IFN)-γ production was observed in PB-γδT cells from TB; however, PE-γδT cells showed a strong response. Both PB- and PE-γδ T cells expressed surface CD107a upon stimulation with Mtb. Notably, PE-γδTCR low cells were the most potent effector cells. Thus, γδT cells from PB would acquire a further activated phenotype within the site of Mtb infection and exert full effector functions. As γδT cells produce IFN-γ within the pleural space, they would be expected to play a beneficial role in tuberculous pleurisy by helping to maintain a T helper type 1 profile. © 2009 British Society for Immunology.
Fil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Schierloh, Luis Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Geffner, Laura Judith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Balboa, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Romero, María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Musella, Rosa María. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Castagnino, J.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: De Stéfano, G.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Alemán, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: de la Barrera, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Abbate, E.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
description Summary Tuberculous pleurisy is a naturally occurring site of Mycobacterium tuberculosis (Mtb) infection. Herein, we describe the expression of activation, natural killer (NK) and cell migration markers, as well as effector functions from γδT cells in peripheral blood (PB) and pleural effusion (PE) from tuberculosis patients (TB). We observed a decreased percentage of circulating γδT from TB patients and differential expression of NK as well as of chemokine receptors on PB and PE. Two subsets of γδT cells were differentiated by the CD3/γδT cell receptor (γδTCR) complex. The γδTCRlow subset had a higher CD3 to TCR ratio and was enriched in Vδ2+ cells, whereas most Vδ1+ cells belonged to the γδTCR high subset. In PB from TB, most γδTCRhigh were CD45RA+CCR7- and γδTCRlow were CD45RA+/-CCR7+CXCR3+. In the pleural space the proportion of CD45RA-CCR7+CXCR3+ cells was higher. Neither spontaneous nor Mtb-induced interferon (IFN)-γ production was observed in PB-γδT cells from TB; however, PE-γδT cells showed a strong response. Both PB- and PE-γδ T cells expressed surface CD107a upon stimulation with Mtb. Notably, PE-γδTCR low cells were the most potent effector cells. Thus, γδT cells from PB would acquire a further activated phenotype within the site of Mtb infection and exert full effector functions. As γδT cells produce IFN-γ within the pleural space, they would be expected to play a beneficial role in tuberculous pleurisy by helping to maintain a T helper type 1 profile. © 2009 British Society for Immunology.
publishDate 2009
dc.date.none.fl_str_mv 2009-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54878
Yokobori, Noemí; Schierloh, Luis Pablo; Geffner, Laura Judith; Balboa, Luciana; Romero, María Mercedes; et al.; CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy; Wiley Blackwell Publishing, Inc; Clinical and Experimental Immunology; 157; 3; 9-2009; 385-394
0009-9104
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54878
identifier_str_mv Yokobori, Noemí; Schierloh, Luis Pablo; Geffner, Laura Judith; Balboa, Luciana; Romero, María Mercedes; et al.; CD3 expression distinguishes two γδT cell receptor subsets with different phenotype and effector function in tuberculous pleurisy; Wiley Blackwell Publishing, Inc; Clinical and Experimental Immunology; 157; 3; 9-2009; 385-394
0009-9104
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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