Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryos

Autores
Vargas, Gabriela Elizabet; Vera Mesones, Rosa; Bretcanu, Oana; Porto Lopez, Jose Manuel; Boccaccini, Aldo R.; Gorustovich Alonso, Alejandro Adrian
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to evaluate the biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds using a chick embryo shell-less (ex ovo) culture system. Chick embryos were divided into two groups: control (C) and experimental (E). Scaffolds were placed on the chorioallantoic membrane (CAM) in embryos of group E at 10 days of total incubation. The 45S5 Bioglass®-derived glass-ceramic scaffolds proved to be biocompatible in terms of the absence of inflammatory response at the implant site (CAM). Moreover, no alterations in the other end-points assessed, i.e. survival, stage of embryonic development and body weight, were detected. However, body length was greater in group E embryos than in group C embryos (p ≤ 0.05). A marked reduction (93%) in Ca content in the scaffolds was evidenced by energy-dispersive X-ray analysis at 5 days post-implantation. Calcium release from the scaffold implanted on the CAM might have been responsible for the restoration of the bone-like phenotype in chick embryonic skeleton of group E as detected by Alcian blue-Alizarin red double staining, as well as by histological and microchemical analyses. Conversely, the control embryos exhibited a chondrogenic phenotype. © 2008 Acta Materialia Inc.
Fil: Vargas, Gabriela Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Vera Mesones, Rosa. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Instituto Geonorte; Argentina
Fil: Bretcanu, Oana. Imperial College London; Reino Unido
Fil: Porto Lopez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Boccaccini, Aldo R.. Imperial College London; Reino Unido
Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Materia
Biocompatibility
Bioglass&Reg;
Chick Embryo
Scaffolds
Tissue Engineering
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66049
Acceder
id CONICETDig_fd0b9a0bceb6fda15f33915eb7b8393d
oai_identifier_str oai:ri.conicet.gov.ar:11336/66049
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryosVargas, Gabriela ElizabetVera Mesones, RosaBretcanu, OanaPorto Lopez, Jose ManuelBoccaccini, Aldo R.Gorustovich Alonso, Alejandro AdrianBiocompatibilityBioglass&Reg;Chick EmbryoScaffoldsTissue Engineeringhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The aim of the present study was to evaluate the biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds using a chick embryo shell-less (ex ovo) culture system. Chick embryos were divided into two groups: control (C) and experimental (E). Scaffolds were placed on the chorioallantoic membrane (CAM) in embryos of group E at 10 days of total incubation. The 45S5 Bioglass®-derived glass-ceramic scaffolds proved to be biocompatible in terms of the absence of inflammatory response at the implant site (CAM). Moreover, no alterations in the other end-points assessed, i.e. survival, stage of embryonic development and body weight, were detected. However, body length was greater in group E embryos than in group C embryos (p ≤ 0.05). A marked reduction (93%) in Ca content in the scaffolds was evidenced by energy-dispersive X-ray analysis at 5 days post-implantation. Calcium release from the scaffold implanted on the CAM might have been responsible for the restoration of the bone-like phenotype in chick embryonic skeleton of group E as detected by Alcian blue-Alizarin red double staining, as well as by histological and microchemical analyses. Conversely, the control embryos exhibited a chondrogenic phenotype. © 2008 Acta Materialia Inc.Fil: Vargas, Gabriela Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Vera Mesones, Rosa. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Instituto Geonorte; ArgentinaFil: Bretcanu, Oana. Imperial College London; Reino UnidoFil: Porto Lopez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Boccaccini, Aldo R.. Imperial College London; Reino UnidoFil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaElsevier2009-01-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66049Vargas, Gabriela Elizabet; Vera Mesones, Rosa; Bretcanu, Oana; Porto Lopez, Jose Manuel; Boccaccini, Aldo R.; et al.; Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryos; Elsevier; Acta Biomaterialia; 5; 1; 31-1-2009; 374-3801742-7061CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1742706108002122info:eu-repo/semantics/altIdentifier/doi/10.1016/j.actbio.2008.07.016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:44Zoai:ri.conicet.gov.ar:11336/66049instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:44.603CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryos
title Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryos
spellingShingle Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryos
Vargas, Gabriela Elizabet
Biocompatibility
Bioglass&Reg;
Chick Embryo
Scaffolds
Tissue Engineering
title_short Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryos
title_full Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryos
title_fullStr Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryos
title_full_unstemmed Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryos
title_sort Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryos
dc.creator.none.fl_str_mv Vargas, Gabriela Elizabet
Vera Mesones, Rosa
Bretcanu, Oana
Porto Lopez, Jose Manuel
Boccaccini, Aldo R.
Gorustovich Alonso, Alejandro Adrian
author Vargas, Gabriela Elizabet
author_facet Vargas, Gabriela Elizabet
Vera Mesones, Rosa
Bretcanu, Oana
Porto Lopez, Jose Manuel
Boccaccini, Aldo R.
Gorustovich Alonso, Alejandro Adrian
author_role author
author2 Vera Mesones, Rosa
Bretcanu, Oana
Porto Lopez, Jose Manuel
Boccaccini, Aldo R.
Gorustovich Alonso, Alejandro Adrian
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biocompatibility
Bioglass&Reg;
Chick Embryo
Scaffolds
Tissue Engineering
topic Biocompatibility
Bioglass&Reg;
Chick Embryo
Scaffolds
Tissue Engineering
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of the present study was to evaluate the biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds using a chick embryo shell-less (ex ovo) culture system. Chick embryos were divided into two groups: control (C) and experimental (E). Scaffolds were placed on the chorioallantoic membrane (CAM) in embryos of group E at 10 days of total incubation. The 45S5 Bioglass®-derived glass-ceramic scaffolds proved to be biocompatible in terms of the absence of inflammatory response at the implant site (CAM). Moreover, no alterations in the other end-points assessed, i.e. survival, stage of embryonic development and body weight, were detected. However, body length was greater in group E embryos than in group C embryos (p ≤ 0.05). A marked reduction (93%) in Ca content in the scaffolds was evidenced by energy-dispersive X-ray analysis at 5 days post-implantation. Calcium release from the scaffold implanted on the CAM might have been responsible for the restoration of the bone-like phenotype in chick embryonic skeleton of group E as detected by Alcian blue-Alizarin red double staining, as well as by histological and microchemical analyses. Conversely, the control embryos exhibited a chondrogenic phenotype. © 2008 Acta Materialia Inc.
Fil: Vargas, Gabriela Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Vera Mesones, Rosa. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Instituto Geonorte; Argentina
Fil: Bretcanu, Oana. Imperial College London; Reino Unido
Fil: Porto Lopez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Boccaccini, Aldo R.. Imperial College London; Reino Unido
Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
description The aim of the present study was to evaluate the biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds using a chick embryo shell-less (ex ovo) culture system. Chick embryos were divided into two groups: control (C) and experimental (E). Scaffolds were placed on the chorioallantoic membrane (CAM) in embryos of group E at 10 days of total incubation. The 45S5 Bioglass®-derived glass-ceramic scaffolds proved to be biocompatible in terms of the absence of inflammatory response at the implant site (CAM). Moreover, no alterations in the other end-points assessed, i.e. survival, stage of embryonic development and body weight, were detected. However, body length was greater in group E embryos than in group C embryos (p ≤ 0.05). A marked reduction (93%) in Ca content in the scaffolds was evidenced by energy-dispersive X-ray analysis at 5 days post-implantation. Calcium release from the scaffold implanted on the CAM might have been responsible for the restoration of the bone-like phenotype in chick embryonic skeleton of group E as detected by Alcian blue-Alizarin red double staining, as well as by histological and microchemical analyses. Conversely, the control embryos exhibited a chondrogenic phenotype. © 2008 Acta Materialia Inc.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66049
Vargas, Gabriela Elizabet; Vera Mesones, Rosa; Bretcanu, Oana; Porto Lopez, Jose Manuel; Boccaccini, Aldo R.; et al.; Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryos; Elsevier; Acta Biomaterialia; 5; 1; 31-1-2009; 374-380
1742-7061
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66049
identifier_str_mv Vargas, Gabriela Elizabet; Vera Mesones, Rosa; Bretcanu, Oana; Porto Lopez, Jose Manuel; Boccaccini, Aldo R.; et al.; Biocompatibility and bone mineralization potential of 45S5 Bioglass®-derived glass-ceramic scaffolds in chick embryos; Elsevier; Acta Biomaterialia; 5; 1; 31-1-2009; 374-380
1742-7061
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1742706108002122
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.actbio.2008.07.016
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613486259208192
score 13.070432

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