Increased AT 1 receptor expression and mRNA in kidney glomeruli of AT 2 receptor gene-disrupted mice

Autores
Saavedra, Juan M.; Häuser, Walter; Ciuffo, Gladys Maria; Egidy, Giorgia; Hoe, Kwang Lae; Jöhren, Olaf; Sembonmatsu, Takaaki; Inagami, Tadashi; Armando, Inés
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The proposed feedback between angiotensin II AT2 and AT1 receptors prompted us to study AT1 receptor expression in kidneys of male AT2 receptor-gene disrupted mice (agtr2 −/y). In wild-type (agtr2 +/y) mice, AT1 receptor binding and mRNA is abundant in glomeruli, and AT1 receptor binding is also high in the inner stripe of the outer medulla. AT2 receptors are scarce, primarily associated to cortical vascular structures. In agtr2 −/y mice, AT1 receptor binding and mRNA were increased in the kidney glomeruli, and AT1 receptor binding was higher in the rest of the cortex and outer stripe of the outer medulla, but not in its inner stripe, indicating different cellular regulation. Although AT2 receptor expression is very low in male agtr 2 +/y mice, their gene disruption alters AT1 receptor expression. AT1 upregulation alone may explain the AT2 gene-disrupted mice phenotype such as increased blood pressure, higher sensitivity to angiotensin II, and altered renal function. The indirect AT1/AT2 receptor feedback could have clinical significance because AT1antagonists are widely used in medical practice.
Fil: Saavedra, Juan M.. National Institute of Mental Health; Estados Unidos
Fil: Häuser, Walter. National Institute of Mental Health; Estados Unidos
Fil: Ciuffo, Gladys Maria. National Institute of Mental Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Egidy, Giorgia. National Institute of Mental Health; Estados Unidos
Fil: Hoe, Kwang Lae. National Institute of Mental Health; Estados Unidos
Fil: Jöhren, Olaf. National Institute of Mental Health; Estados Unidos
Fil: Sembonmatsu, Takaaki. Vanderbilt University; Estados Unidos
Fil: Inagami, Tadashi. Vanderbilt University; Estados Unidos
Fil: Armando, Inés. National Institute of Mental Health; Estados Unidos
Materia
Renin-angiotensin system
Angiotensin II receptors types
Gene-disrupted models
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/136383
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/136383
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased AT 1 receptor expression and mRNA in kidney glomeruli of AT 2 receptor gene-disrupted miceSaavedra, Juan M.Häuser, WalterCiuffo, Gladys MariaEgidy, GiorgiaHoe, Kwang LaeJöhren, OlafSembonmatsu, TakaakiInagami, TadashiArmando, InésRenin-angiotensin systemAngiotensin II receptors typesGene-disrupted modelshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The proposed feedback between angiotensin II AT2 and AT1 receptors prompted us to study AT1 receptor expression in kidneys of male AT2 receptor-gene disrupted mice (agtr2 −/y). In wild-type (agtr2 +/y) mice, AT1 receptor binding and mRNA is abundant in glomeruli, and AT1 receptor binding is also high in the inner stripe of the outer medulla. AT2 receptors are scarce, primarily associated to cortical vascular structures. In agtr2 −/y mice, AT1 receptor binding and mRNA were increased in the kidney glomeruli, and AT1 receptor binding was higher in the rest of the cortex and outer stripe of the outer medulla, but not in its inner stripe, indicating different cellular regulation. Although AT2 receptor expression is very low in male agtr 2 +/y mice, their gene disruption alters AT1 receptor expression. AT1 upregulation alone may explain the AT2 gene-disrupted mice phenotype such as increased blood pressure, higher sensitivity to angiotensin II, and altered renal function. The indirect AT1/AT2 receptor feedback could have clinical significance because AT1antagonists are widely used in medical practice.Fil: Saavedra, Juan M.. National Institute of Mental Health; Estados UnidosFil: Häuser, Walter. National Institute of Mental Health; Estados UnidosFil: Ciuffo, Gladys Maria. National Institute of Mental Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Egidy, Giorgia. National Institute of Mental Health; Estados UnidosFil: Hoe, Kwang Lae. National Institute of Mental Health; Estados UnidosFil: Jöhren, Olaf. National Institute of Mental Health; Estados UnidosFil: Sembonmatsu, Takaaki. Vanderbilt University; Estados UnidosFil: Inagami, Tadashi. Vanderbilt University; Estados UnidosFil: Armando, Inés. National Institute of Mental Health; Estados UnidosAmerican Physiological Society2001-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/136383Saavedra, Juan M.; Häuser, Walter; Ciuffo, Gladys Maria; Egidy, Giorgia; Hoe, Kwang Lae; et al.; Increased AT 1 receptor expression and mRNA in kidney glomeruli of AT 2 receptor gene-disrupted mice; American Physiological Society; American Journal Of Physiology-renal Physiology; 280; 1; 1-2001; F71-F781931-857X1522-1466CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajprenal.2001.280.1.F71info:eu-repo/semantics/altIdentifier/doi/10.1152/ajprenal.2001.280.1.F71info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:49Zoai:ri.conicet.gov.ar:11336/136383instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:49.65CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased AT 1 receptor expression and mRNA in kidney glomeruli of AT 2 receptor gene-disrupted mice
title Increased AT 1 receptor expression and mRNA in kidney glomeruli of AT 2 receptor gene-disrupted mice
spellingShingle Increased AT 1 receptor expression and mRNA in kidney glomeruli of AT 2 receptor gene-disrupted mice
Saavedra, Juan M.
Renin-angiotensin system
Angiotensin II receptors types
Gene-disrupted models
title_short Increased AT 1 receptor expression and mRNA in kidney glomeruli of AT 2 receptor gene-disrupted mice
title_full Increased AT 1 receptor expression and mRNA in kidney glomeruli of AT 2 receptor gene-disrupted mice
title_fullStr Increased AT 1 receptor expression and mRNA in kidney glomeruli of AT 2 receptor gene-disrupted mice
title_full_unstemmed Increased AT 1 receptor expression and mRNA in kidney glomeruli of AT 2 receptor gene-disrupted mice
title_sort Increased AT 1 receptor expression and mRNA in kidney glomeruli of AT 2 receptor gene-disrupted mice
dc.creator.none.fl_str_mv Saavedra, Juan M.
Häuser, Walter
Ciuffo, Gladys Maria
Egidy, Giorgia
Hoe, Kwang Lae
Jöhren, Olaf
Sembonmatsu, Takaaki
Inagami, Tadashi
Armando, Inés
author Saavedra, Juan M.
author_facet Saavedra, Juan M.
Häuser, Walter
Ciuffo, Gladys Maria
Egidy, Giorgia
Hoe, Kwang Lae
Jöhren, Olaf
Sembonmatsu, Takaaki
Inagami, Tadashi
Armando, Inés
author_role author
author2 Häuser, Walter
Ciuffo, Gladys Maria
Egidy, Giorgia
Hoe, Kwang Lae
Jöhren, Olaf
Sembonmatsu, Takaaki
Inagami, Tadashi
Armando, Inés
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Renin-angiotensin system
Angiotensin II receptors types
Gene-disrupted models
topic Renin-angiotensin system
Angiotensin II receptors types
Gene-disrupted models
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The proposed feedback between angiotensin II AT2 and AT1 receptors prompted us to study AT1 receptor expression in kidneys of male AT2 receptor-gene disrupted mice (agtr2 −/y). In wild-type (agtr2 +/y) mice, AT1 receptor binding and mRNA is abundant in glomeruli, and AT1 receptor binding is also high in the inner stripe of the outer medulla. AT2 receptors are scarce, primarily associated to cortical vascular structures. In agtr2 −/y mice, AT1 receptor binding and mRNA were increased in the kidney glomeruli, and AT1 receptor binding was higher in the rest of the cortex and outer stripe of the outer medulla, but not in its inner stripe, indicating different cellular regulation. Although AT2 receptor expression is very low in male agtr 2 +/y mice, their gene disruption alters AT1 receptor expression. AT1 upregulation alone may explain the AT2 gene-disrupted mice phenotype such as increased blood pressure, higher sensitivity to angiotensin II, and altered renal function. The indirect AT1/AT2 receptor feedback could have clinical significance because AT1antagonists are widely used in medical practice.
Fil: Saavedra, Juan M.. National Institute of Mental Health; Estados Unidos
Fil: Häuser, Walter. National Institute of Mental Health; Estados Unidos
Fil: Ciuffo, Gladys Maria. National Institute of Mental Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Egidy, Giorgia. National Institute of Mental Health; Estados Unidos
Fil: Hoe, Kwang Lae. National Institute of Mental Health; Estados Unidos
Fil: Jöhren, Olaf. National Institute of Mental Health; Estados Unidos
Fil: Sembonmatsu, Takaaki. Vanderbilt University; Estados Unidos
Fil: Inagami, Tadashi. Vanderbilt University; Estados Unidos
Fil: Armando, Inés. National Institute of Mental Health; Estados Unidos
description The proposed feedback between angiotensin II AT2 and AT1 receptors prompted us to study AT1 receptor expression in kidneys of male AT2 receptor-gene disrupted mice (agtr2 −/y). In wild-type (agtr2 +/y) mice, AT1 receptor binding and mRNA is abundant in glomeruli, and AT1 receptor binding is also high in the inner stripe of the outer medulla. AT2 receptors are scarce, primarily associated to cortical vascular structures. In agtr2 −/y mice, AT1 receptor binding and mRNA were increased in the kidney glomeruli, and AT1 receptor binding was higher in the rest of the cortex and outer stripe of the outer medulla, but not in its inner stripe, indicating different cellular regulation. Although AT2 receptor expression is very low in male agtr 2 +/y mice, their gene disruption alters AT1 receptor expression. AT1 upregulation alone may explain the AT2 gene-disrupted mice phenotype such as increased blood pressure, higher sensitivity to angiotensin II, and altered renal function. The indirect AT1/AT2 receptor feedback could have clinical significance because AT1antagonists are widely used in medical practice.
publishDate 2001
dc.date.none.fl_str_mv 2001-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/136383
Saavedra, Juan M.; Häuser, Walter; Ciuffo, Gladys Maria; Egidy, Giorgia; Hoe, Kwang Lae; et al.; Increased AT 1 receptor expression and mRNA in kidney glomeruli of AT 2 receptor gene-disrupted mice; American Physiological Society; American Journal Of Physiology-renal Physiology; 280; 1; 1-2001; F71-F78
1931-857X
1522-1466
CONICET Digital
CONICET
url http://hdl.handle.net/11336/136383
identifier_str_mv Saavedra, Juan M.; Häuser, Walter; Ciuffo, Gladys Maria; Egidy, Giorgia; Hoe, Kwang Lae; et al.; Increased AT 1 receptor expression and mRNA in kidney glomeruli of AT 2 receptor gene-disrupted mice; American Physiological Society; American Journal Of Physiology-renal Physiology; 280; 1; 1-2001; F71-F78
1931-857X
1522-1466
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajprenal.2001.280.1.F71
info:eu-repo/semantics/altIdentifier/doi/10.1152/ajprenal.2001.280.1.F71
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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