Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monol...

Autores
Acquaviva, Agustín; Romero, Lilian; Castells, Cecilia Beatriz Marta; Ramis Ramos, Guillermo; Herrero Martinez, José Manuel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We describe the development of a high-performance liquid chromatography (HPLC) method for the determination of citrulline and other amino acids relevant to intestinal diseases. The amino acids were derivatized with 9-fluorenylmethylchloroformate (FMOC-Cl) and their derivatives were separated on two different columns, a core-shell column (Halo C18) and a silica-based monolith (Chromolith Performance RP-18). The derivatization reaction was optimized with respect to pH, buffer concentration and reproducibility. The optimal derivatization conditions were achieved with 0.4 M borate buffer at pH 9.20, a constant ratio of FMOC-Cl/total amino acids (10 : 1) and 75 mM tyramine after 1 min (quenching reaction). The separation conditions with both chromatographic supports were also optimized. The chromatographic performance (peak capacity and global resolution) of these two columns was compared. This proposed HPLC-UV method was satisfactorily applied to the analysis of a real plasma sample. © 2014 The Royal Society of Chemistry.
Fil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Lilian. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos ; Argentina
Fil: Castells, Cecilia Beatriz Marta. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos ; Argentina
Fil: Ramis Ramos, Guillermo. Universidad de Valencia; España
Fil: Herrero Martinez, José Manuel. Universidad de Valencia; España
Materia
Amino Acids
Citrulline
Core-Shell Columns
Monolithic Columns
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11322
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11322
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monolithic and core–shell columnsAcquaviva, AgustínRomero, LilianCastells, Cecilia Beatriz MartaRamis Ramos, GuillermoHerrero Martinez, José ManuelAmino AcidsCitrullineCore-Shell ColumnsMonolithic Columnshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We describe the development of a high-performance liquid chromatography (HPLC) method for the determination of citrulline and other amino acids relevant to intestinal diseases. The amino acids were derivatized with 9-fluorenylmethylchloroformate (FMOC-Cl) and their derivatives were separated on two different columns, a core-shell column (Halo C18) and a silica-based monolith (Chromolith Performance RP-18). The derivatization reaction was optimized with respect to pH, buffer concentration and reproducibility. The optimal derivatization conditions were achieved with 0.4 M borate buffer at pH 9.20, a constant ratio of FMOC-Cl/total amino acids (10 : 1) and 75 mM tyramine after 1 min (quenching reaction). The separation conditions with both chromatographic supports were also optimized. The chromatographic performance (peak capacity and global resolution) of these two columns was compared. This proposed HPLC-UV method was satisfactorily applied to the analysis of a real plasma sample. © 2014 The Royal Society of Chemistry.Fil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romero, Lilian. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos ; ArgentinaFil: Castells, Cecilia Beatriz Marta. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos ; ArgentinaFil: Ramis Ramos, Guillermo. Universidad de Valencia; EspañaFil: Herrero Martinez, José Manuel. Universidad de Valencia; EspañaRoyal Society of Chemistry2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11322Acquaviva, Agustín; Romero, Lilian; Castells, Cecilia Beatriz Marta; Ramis Ramos, Guillermo; Herrero Martinez, José Manuel; Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monolithic and core–shell columns; Royal Society of Chemistry; Analytical Methods; 6; 15; 8-2014; 5830-58371759-96791759-9660enginfo:eu-repo/semantics/altIdentifier/doi/10.1039/c4ay00496einfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2014/AY/C4AY00496E#!divAbstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:44:25Zoai:ri.conicet.gov.ar:11336/11322instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:44:25.68CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monolithic and core–shell columns
title Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monolithic and core–shell columns
spellingShingle Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monolithic and core–shell columns
Acquaviva, Agustín
Amino Acids
Citrulline
Core-Shell Columns
Monolithic Columns
title_short Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monolithic and core–shell columns
title_full Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monolithic and core–shell columns
title_fullStr Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monolithic and core–shell columns
title_full_unstemmed Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monolithic and core–shell columns
title_sort Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monolithic and core–shell columns
dc.creator.none.fl_str_mv Acquaviva, Agustín
Romero, Lilian
Castells, Cecilia Beatriz Marta
Ramis Ramos, Guillermo
Herrero Martinez, José Manuel
author Acquaviva, Agustín
author_facet Acquaviva, Agustín
Romero, Lilian
Castells, Cecilia Beatriz Marta
Ramis Ramos, Guillermo
Herrero Martinez, José Manuel
author_role author
author2 Romero, Lilian
Castells, Cecilia Beatriz Marta
Ramis Ramos, Guillermo
Herrero Martinez, José Manuel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Amino Acids
Citrulline
Core-Shell Columns
Monolithic Columns
topic Amino Acids
Citrulline
Core-Shell Columns
Monolithic Columns
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We describe the development of a high-performance liquid chromatography (HPLC) method for the determination of citrulline and other amino acids relevant to intestinal diseases. The amino acids were derivatized with 9-fluorenylmethylchloroformate (FMOC-Cl) and their derivatives were separated on two different columns, a core-shell column (Halo C18) and a silica-based monolith (Chromolith Performance RP-18). The derivatization reaction was optimized with respect to pH, buffer concentration and reproducibility. The optimal derivatization conditions were achieved with 0.4 M borate buffer at pH 9.20, a constant ratio of FMOC-Cl/total amino acids (10 : 1) and 75 mM tyramine after 1 min (quenching reaction). The separation conditions with both chromatographic supports were also optimized. The chromatographic performance (peak capacity and global resolution) of these two columns was compared. This proposed HPLC-UV method was satisfactorily applied to the analysis of a real plasma sample. © 2014 The Royal Society of Chemistry.
Fil: Acquaviva, Agustín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Lilian. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos ; Argentina
Fil: Castells, Cecilia Beatriz Marta. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos ; Argentina
Fil: Ramis Ramos, Guillermo. Universidad de Valencia; España
Fil: Herrero Martinez, José Manuel. Universidad de Valencia; España
description We describe the development of a high-performance liquid chromatography (HPLC) method for the determination of citrulline and other amino acids relevant to intestinal diseases. The amino acids were derivatized with 9-fluorenylmethylchloroformate (FMOC-Cl) and their derivatives were separated on two different columns, a core-shell column (Halo C18) and a silica-based monolith (Chromolith Performance RP-18). The derivatization reaction was optimized with respect to pH, buffer concentration and reproducibility. The optimal derivatization conditions were achieved with 0.4 M borate buffer at pH 9.20, a constant ratio of FMOC-Cl/total amino acids (10 : 1) and 75 mM tyramine after 1 min (quenching reaction). The separation conditions with both chromatographic supports were also optimized. The chromatographic performance (peak capacity and global resolution) of these two columns was compared. This proposed HPLC-UV method was satisfactorily applied to the analysis of a real plasma sample. © 2014 The Royal Society of Chemistry.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11322
Acquaviva, Agustín; Romero, Lilian; Castells, Cecilia Beatriz Marta; Ramis Ramos, Guillermo; Herrero Martinez, José Manuel; Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monolithic and core–shell columns; Royal Society of Chemistry; Analytical Methods; 6; 15; 8-2014; 5830-5837
1759-9679
1759-9660
url http://hdl.handle.net/11336/11322
identifier_str_mv Acquaviva, Agustín; Romero, Lilian; Castells, Cecilia Beatriz Marta; Ramis Ramos, Guillermo; Herrero Martinez, José Manuel; Reliable and simple analytical methods for determination of citrulline and metabolically related amino acids by liquid chromatography after derivatization: comparison between monolithic and core–shell columns; Royal Society of Chemistry; Analytical Methods; 6; 15; 8-2014; 5830-5837
1759-9679
1759-9660
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/c4ay00496e
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2014/AY/C4AY00496E#!divAbstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082954794631168
score 13.22299

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