Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis

Autores
Olmos Carreño, Cindy Lorena; Figueres Oñate, María; Scicolone, Gabriel Edgardo; López Mascaraque, Laura
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Clonal cell analysis outlines the ontogenic potential of single progenitor cells, allowing the elucidation of the neural heterogeneity among different cell types and their lineages. In this work, we analyze the potency of retinal stem/progenitor cells through development using the chick embryo as a model. We implemented in ovo the clonal genetic tracing strategy UbC-StarTrack for tracking retinal cell lineages derived from individual progenitors of the ciliary margin at E3.5 (HH21-22). The clonal assignment of the derived-cell progeny was performed in the neural retina at E11.5-12 (HH38) through the identification of sibling cells as cells expressing the same combination of fluorophores. Moreover, cell types were assessed based on their cellular morphology and laminar location. Ciliary margin derived-cell progenies are organized in columnar associations distributed along the peripheral retina with a limited tangential dispersion. The analysis revealed that, at the early stages of development, this region harbors multipotent and committed progenitor cells.
Fil: Olmos Carreño, Cindy Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Figueres Oñate, María. INSTITUTO CAJAL ; CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS;
Fil: Scicolone, Gabriel Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: López Mascaraque, Laura. INSTITUTO CAJAL ; CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS;
Materia
CHICK EMBRYO
CILIARY MARGIN
CLONAL ANALYSIS
STEM/PROGENITOR RETINAL CELLS
UBC-STARTRACK
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/214291
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/214291
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysisOlmos Carreño, Cindy LorenaFigueres Oñate, MaríaScicolone, Gabriel EdgardoLópez Mascaraque, LauraCHICK EMBRYOCILIARY MARGINCLONAL ANALYSISSTEM/PROGENITOR RETINAL CELLSUBC-STARTRACKhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Clonal cell analysis outlines the ontogenic potential of single progenitor cells, allowing the elucidation of the neural heterogeneity among different cell types and their lineages. In this work, we analyze the potency of retinal stem/progenitor cells through development using the chick embryo as a model. We implemented in ovo the clonal genetic tracing strategy UbC-StarTrack for tracking retinal cell lineages derived from individual progenitors of the ciliary margin at E3.5 (HH21-22). The clonal assignment of the derived-cell progeny was performed in the neural retina at E11.5-12 (HH38) through the identification of sibling cells as cells expressing the same combination of fluorophores. Moreover, cell types were assessed based on their cellular morphology and laminar location. Ciliary margin derived-cell progenies are organized in columnar associations distributed along the peripheral retina with a limited tangential dispersion. The analysis revealed that, at the early stages of development, this region harbors multipotent and committed progenitor cells.Fil: Olmos Carreño, Cindy Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Figueres Oñate, María. INSTITUTO CAJAL ; CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS;Fil: Scicolone, Gabriel Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: López Mascaraque, Laura. INSTITUTO CAJAL ; CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS;Molecular Diversity Preservation International2022-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214291Olmos Carreño, Cindy Lorena; Figueres Oñate, María; Scicolone, Gabriel Edgardo; López Mascaraque, Laura; Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 23; 20; 10-2022; 1-161422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/ 10.3390/ijms232012388info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:44:17Zoai:ri.conicet.gov.ar:11336/214291instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:44:17.481CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis
title Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis
spellingShingle Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis
Olmos Carreño, Cindy Lorena
CHICK EMBRYO
CILIARY MARGIN
CLONAL ANALYSIS
STEM/PROGENITOR RETINAL CELLS
UBC-STARTRACK
title_short Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis
title_full Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis
title_fullStr Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis
title_full_unstemmed Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis
title_sort Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis
dc.creator.none.fl_str_mv Olmos Carreño, Cindy Lorena
Figueres Oñate, María
Scicolone, Gabriel Edgardo
López Mascaraque, Laura
author Olmos Carreño, Cindy Lorena
author_facet Olmos Carreño, Cindy Lorena
Figueres Oñate, María
Scicolone, Gabriel Edgardo
López Mascaraque, Laura
author_role author
author2 Figueres Oñate, María
Scicolone, Gabriel Edgardo
López Mascaraque, Laura
author2_role author
author
author
dc.subject.none.fl_str_mv CHICK EMBRYO
CILIARY MARGIN
CLONAL ANALYSIS
STEM/PROGENITOR RETINAL CELLS
UBC-STARTRACK
topic CHICK EMBRYO
CILIARY MARGIN
CLONAL ANALYSIS
STEM/PROGENITOR RETINAL CELLS
UBC-STARTRACK
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Clonal cell analysis outlines the ontogenic potential of single progenitor cells, allowing the elucidation of the neural heterogeneity among different cell types and their lineages. In this work, we analyze the potency of retinal stem/progenitor cells through development using the chick embryo as a model. We implemented in ovo the clonal genetic tracing strategy UbC-StarTrack for tracking retinal cell lineages derived from individual progenitors of the ciliary margin at E3.5 (HH21-22). The clonal assignment of the derived-cell progeny was performed in the neural retina at E11.5-12 (HH38) through the identification of sibling cells as cells expressing the same combination of fluorophores. Moreover, cell types were assessed based on their cellular morphology and laminar location. Ciliary margin derived-cell progenies are organized in columnar associations distributed along the peripheral retina with a limited tangential dispersion. The analysis revealed that, at the early stages of development, this region harbors multipotent and committed progenitor cells.
Fil: Olmos Carreño, Cindy Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Figueres Oñate, María. INSTITUTO CAJAL ; CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS;
Fil: Scicolone, Gabriel Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: López Mascaraque, Laura. INSTITUTO CAJAL ; CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS;
description Clonal cell analysis outlines the ontogenic potential of single progenitor cells, allowing the elucidation of the neural heterogeneity among different cell types and their lineages. In this work, we analyze the potency of retinal stem/progenitor cells through development using the chick embryo as a model. We implemented in ovo the clonal genetic tracing strategy UbC-StarTrack for tracking retinal cell lineages derived from individual progenitors of the ciliary margin at E3.5 (HH21-22). The clonal assignment of the derived-cell progeny was performed in the neural retina at E11.5-12 (HH38) through the identification of sibling cells as cells expressing the same combination of fluorophores. Moreover, cell types were assessed based on their cellular morphology and laminar location. Ciliary margin derived-cell progenies are organized in columnar associations distributed along the peripheral retina with a limited tangential dispersion. The analysis revealed that, at the early stages of development, this region harbors multipotent and committed progenitor cells.
publishDate 2022
dc.date.none.fl_str_mv 2022-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/214291
Olmos Carreño, Cindy Lorena; Figueres Oñate, María; Scicolone, Gabriel Edgardo; López Mascaraque, Laura; Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 23; 20; 10-2022; 1-16
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/214291
identifier_str_mv Olmos Carreño, Cindy Lorena; Figueres Oñate, María; Scicolone, Gabriel Edgardo; López Mascaraque, Laura; Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 23; 20; 10-2022; 1-16
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/ 10.3390/ijms232012388
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782137297010688
score 12.982451

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