Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis
- Autores
- Olmos Carreño, Cindy Lorena; Figueres Oñate, María; Scicolone, Gabriel Edgardo; López Mascaraque, Laura
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Clonal cell analysis outlines the ontogenic potential of single progenitor cells, allowing the elucidation of the neural heterogeneity among different cell types and their lineages. In this work, we analyze the potency of retinal stem/progenitor cells through development using the chick embryo as a model. We implemented in ovo the clonal genetic tracing strategy UbC-StarTrack for tracking retinal cell lineages derived from individual progenitors of the ciliary margin at E3.5 (HH21-22). The clonal assignment of the derived-cell progeny was performed in the neural retina at E11.5-12 (HH38) through the identification of sibling cells as cells expressing the same combination of fluorophores. Moreover, cell types were assessed based on their cellular morphology and laminar location. Ciliary margin derived-cell progenies are organized in columnar associations distributed along the peripheral retina with a limited tangential dispersion. The analysis revealed that, at the early stages of development, this region harbors multipotent and committed progenitor cells.
Fil: Olmos Carreño, Cindy Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Figueres Oñate, María. INSTITUTO CAJAL ; CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS;
Fil: Scicolone, Gabriel Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: López Mascaraque, Laura. INSTITUTO CAJAL ; CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS; - Materia
-
CHICK EMBRYO
CILIARY MARGIN
CLONAL ANALYSIS
STEM/PROGENITOR RETINAL CELLS
UBC-STARTRACK - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/214291
Ver los metadatos del registro completo
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Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysisOlmos Carreño, Cindy LorenaFigueres Oñate, MaríaScicolone, Gabriel EdgardoLópez Mascaraque, LauraCHICK EMBRYOCILIARY MARGINCLONAL ANALYSISSTEM/PROGENITOR RETINAL CELLSUBC-STARTRACKhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Clonal cell analysis outlines the ontogenic potential of single progenitor cells, allowing the elucidation of the neural heterogeneity among different cell types and their lineages. In this work, we analyze the potency of retinal stem/progenitor cells through development using the chick embryo as a model. We implemented in ovo the clonal genetic tracing strategy UbC-StarTrack for tracking retinal cell lineages derived from individual progenitors of the ciliary margin at E3.5 (HH21-22). The clonal assignment of the derived-cell progeny was performed in the neural retina at E11.5-12 (HH38) through the identification of sibling cells as cells expressing the same combination of fluorophores. Moreover, cell types were assessed based on their cellular morphology and laminar location. Ciliary margin derived-cell progenies are organized in columnar associations distributed along the peripheral retina with a limited tangential dispersion. The analysis revealed that, at the early stages of development, this region harbors multipotent and committed progenitor cells.Fil: Olmos Carreño, Cindy Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Figueres Oñate, María. INSTITUTO CAJAL ; CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS;Fil: Scicolone, Gabriel Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: López Mascaraque, Laura. INSTITUTO CAJAL ; CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS;Molecular Diversity Preservation International2022-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214291Olmos Carreño, Cindy Lorena; Figueres Oñate, María; Scicolone, Gabriel Edgardo; López Mascaraque, Laura; Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 23; 20; 10-2022; 1-161422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/ 10.3390/ijms232012388info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:15Zoai:ri.conicet.gov.ar:11336/214291instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:15.662CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis |
title |
Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis |
spellingShingle |
Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis Olmos Carreño, Cindy Lorena CHICK EMBRYO CILIARY MARGIN CLONAL ANALYSIS STEM/PROGENITOR RETINAL CELLS UBC-STARTRACK |
title_short |
Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis |
title_full |
Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis |
title_fullStr |
Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis |
title_full_unstemmed |
Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis |
title_sort |
Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis |
dc.creator.none.fl_str_mv |
Olmos Carreño, Cindy Lorena Figueres Oñate, María Scicolone, Gabriel Edgardo López Mascaraque, Laura |
author |
Olmos Carreño, Cindy Lorena |
author_facet |
Olmos Carreño, Cindy Lorena Figueres Oñate, María Scicolone, Gabriel Edgardo López Mascaraque, Laura |
author_role |
author |
author2 |
Figueres Oñate, María Scicolone, Gabriel Edgardo López Mascaraque, Laura |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
CHICK EMBRYO CILIARY MARGIN CLONAL ANALYSIS STEM/PROGENITOR RETINAL CELLS UBC-STARTRACK |
topic |
CHICK EMBRYO CILIARY MARGIN CLONAL ANALYSIS STEM/PROGENITOR RETINAL CELLS UBC-STARTRACK |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Clonal cell analysis outlines the ontogenic potential of single progenitor cells, allowing the elucidation of the neural heterogeneity among different cell types and their lineages. In this work, we analyze the potency of retinal stem/progenitor cells through development using the chick embryo as a model. We implemented in ovo the clonal genetic tracing strategy UbC-StarTrack for tracking retinal cell lineages derived from individual progenitors of the ciliary margin at E3.5 (HH21-22). The clonal assignment of the derived-cell progeny was performed in the neural retina at E11.5-12 (HH38) through the identification of sibling cells as cells expressing the same combination of fluorophores. Moreover, cell types were assessed based on their cellular morphology and laminar location. Ciliary margin derived-cell progenies are organized in columnar associations distributed along the peripheral retina with a limited tangential dispersion. The analysis revealed that, at the early stages of development, this region harbors multipotent and committed progenitor cells. Fil: Olmos Carreño, Cindy Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina Fil: Figueres Oñate, María. INSTITUTO CAJAL ; CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS; Fil: Scicolone, Gabriel Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina Fil: López Mascaraque, Laura. INSTITUTO CAJAL ; CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS; |
description |
Clonal cell analysis outlines the ontogenic potential of single progenitor cells, allowing the elucidation of the neural heterogeneity among different cell types and their lineages. In this work, we analyze the potency of retinal stem/progenitor cells through development using the chick embryo as a model. We implemented in ovo the clonal genetic tracing strategy UbC-StarTrack for tracking retinal cell lineages derived from individual progenitors of the ciliary margin at E3.5 (HH21-22). The clonal assignment of the derived-cell progeny was performed in the neural retina at E11.5-12 (HH38) through the identification of sibling cells as cells expressing the same combination of fluorophores. Moreover, cell types were assessed based on their cellular morphology and laminar location. Ciliary margin derived-cell progenies are organized in columnar associations distributed along the peripheral retina with a limited tangential dispersion. The analysis revealed that, at the early stages of development, this region harbors multipotent and committed progenitor cells. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/214291 Olmos Carreño, Cindy Lorena; Figueres Oñate, María; Scicolone, Gabriel Edgardo; López Mascaraque, Laura; Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 23; 20; 10-2022; 1-16 1422-0067 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/214291 |
identifier_str_mv |
Olmos Carreño, Cindy Lorena; Figueres Oñate, María; Scicolone, Gabriel Edgardo; López Mascaraque, Laura; Cell fate of retinal progenitor cells: In ovo UbC-SarTrack analysis; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 23; 20; 10-2022; 1-16 1422-0067 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/ 10.3390/ijms232012388 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614068052164608 |
score |
13.070432 |