Development and validation of a Capillary Zone Electrophoresis method for the determination of calcium in composite biomaterials

Autores
Cattalini, Juan Pablo; García, Javier; Mouriño, Viviana Silvia Lourdes; Lucangioli, Silvia Edith
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work presents the development and validation of a capillary zone electrophoresis method for calcium ions release quantitation from composite biomaterials with potential application in bone tissue engineering. Calcium ions quantitation was made using a fused silica capillary (40 cm, 75 µm ID) and background electrolyte containing 5 mM imidazole, 6 mM á-hydroxyisobutyric acid, 1 mM 1,4,7,10,13,16-hexaoxacyclo-octadecane and 20 % w/v methanol, at pH 4.5. Indirect UV-detection mode at 214 nm was performed. The separations were achieved using normal polarity at 6 kV, a cartridge temperature of 25 µC and 0.5 psi for 5 s for sample introduction. Parameters of validation such as specificity, linearity, limit of detection and quantitation, accuracy, precision and robustness were evaluated according to the International Conference on Harmonization guidelines. The new developed method resulted to be suitable for calcium determination from composite biomaterials for potential application in bone tissue engineering.
Fil: Cattalini, Juan Pablo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
Fil: García, Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina;
Fil: Mouriño, Viviana Silvia Lourdes. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
Materia
CALCIUM QUANTITATION
CAPILLARY ZONE ELECTROPHORESIS
COMPOSITE MATERIALS
INDIRECT UV
IONS RELEASE
VALIDATION
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1924
Acceder
id CONICETDig_fc816ef35ee2b9a78454885fc4611991
oai_identifier_str oai:ri.conicet.gov.ar:11336/1924
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development and validation of a Capillary Zone Electrophoresis method for the determination of calcium in composite biomaterialsCattalini, Juan PabloGarcía, JavierMouriño, Viviana Silvia LourdesLucangioli, Silvia EdithCALCIUM QUANTITATIONCAPILLARY ZONE ELECTROPHORESISCOMPOSITE MATERIALSINDIRECT UVIONS RELEASEVALIDATIONhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3This work presents the development and validation of a capillary zone electrophoresis method for calcium ions release quantitation from composite biomaterials with potential application in bone tissue engineering. Calcium ions quantitation was made using a fused silica capillary (40 cm, 75 µm ID) and background electrolyte containing 5 mM imidazole, 6 mM á-hydroxyisobutyric acid, 1 mM 1,4,7,10,13,16-hexaoxacyclo-octadecane and 20 % w/v methanol, at pH 4.5. Indirect UV-detection mode at 214 nm was performed. The separations were achieved using normal polarity at 6 kV, a cartridge temperature of 25 µC and 0.5 psi for 5 s for sample introduction. Parameters of validation such as specificity, linearity, limit of detection and quantitation, accuracy, precision and robustness were evaluated according to the International Conference on Harmonization guidelines. The new developed method resulted to be suitable for calcium determination from composite biomaterials for potential application in bone tissue engineering.Fil: Cattalini, Juan Pablo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;Fil: García, Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina;Fil: Mouriño, Viviana Silvia Lourdes. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;Bentham Science Publishers2014-10info:eu-repo/date/embargoEnd/2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1924Cattalini, Juan Pablo; García, Javier; Mouriño, Viviana Silvia Lourdes; Lucangioli, Silvia Edith; Development and validation of a Capillary Zone Electrophoresis method for the determination of calcium in composite biomaterials; Bentham Science Publishers; Current Analytical Chemistry; 10; 4; 10-2014; 465-4721573-4110http://www.ingentaconnect.com/content/ben/cac/2014/00000010/00000004/art00004?crawler=true#expand/collapseenginfo:eu-repo/semantics/altIdentifier/doi/ 10.2174/15734110113099990038info:eu-repo/semantics/altIdentifier/url/http://benthamscience.com/journals/current-analytical-chemistry/volume/10/issue/4/page/465/info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:05:34Zoai:ri.conicet.gov.ar:11336/1924instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:05:34.532CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development and validation of a Capillary Zone Electrophoresis method for the determination of calcium in composite biomaterials
title Development and validation of a Capillary Zone Electrophoresis method for the determination of calcium in composite biomaterials
spellingShingle Development and validation of a Capillary Zone Electrophoresis method for the determination of calcium in composite biomaterials
Cattalini, Juan Pablo
CALCIUM QUANTITATION
CAPILLARY ZONE ELECTROPHORESIS
COMPOSITE MATERIALS
INDIRECT UV
IONS RELEASE
VALIDATION
title_short Development and validation of a Capillary Zone Electrophoresis method for the determination of calcium in composite biomaterials
title_full Development and validation of a Capillary Zone Electrophoresis method for the determination of calcium in composite biomaterials
title_fullStr Development and validation of a Capillary Zone Electrophoresis method for the determination of calcium in composite biomaterials
title_full_unstemmed Development and validation of a Capillary Zone Electrophoresis method for the determination of calcium in composite biomaterials
title_sort Development and validation of a Capillary Zone Electrophoresis method for the determination of calcium in composite biomaterials
dc.creator.none.fl_str_mv Cattalini, Juan Pablo
García, Javier
Mouriño, Viviana Silvia Lourdes
Lucangioli, Silvia Edith
author Cattalini, Juan Pablo
author_facet Cattalini, Juan Pablo
García, Javier
Mouriño, Viviana Silvia Lourdes
Lucangioli, Silvia Edith
author_role author
author2 García, Javier
Mouriño, Viviana Silvia Lourdes
Lucangioli, Silvia Edith
author2_role author
author
author
dc.subject.none.fl_str_mv CALCIUM QUANTITATION
CAPILLARY ZONE ELECTROPHORESIS
COMPOSITE MATERIALS
INDIRECT UV
IONS RELEASE
VALIDATION
topic CALCIUM QUANTITATION
CAPILLARY ZONE ELECTROPHORESIS
COMPOSITE MATERIALS
INDIRECT UV
IONS RELEASE
VALIDATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv This work presents the development and validation of a capillary zone electrophoresis method for calcium ions release quantitation from composite biomaterials with potential application in bone tissue engineering. Calcium ions quantitation was made using a fused silica capillary (40 cm, 75 µm ID) and background electrolyte containing 5 mM imidazole, 6 mM á-hydroxyisobutyric acid, 1 mM 1,4,7,10,13,16-hexaoxacyclo-octadecane and 20 % w/v methanol, at pH 4.5. Indirect UV-detection mode at 214 nm was performed. The separations were achieved using normal polarity at 6 kV, a cartridge temperature of 25 µC and 0.5 psi for 5 s for sample introduction. Parameters of validation such as specificity, linearity, limit of detection and quantitation, accuracy, precision and robustness were evaluated according to the International Conference on Harmonization guidelines. The new developed method resulted to be suitable for calcium determination from composite biomaterials for potential application in bone tissue engineering.
Fil: Cattalini, Juan Pablo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
Fil: García, Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina;
Fil: Mouriño, Viviana Silvia Lourdes. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
description This work presents the development and validation of a capillary zone electrophoresis method for calcium ions release quantitation from composite biomaterials with potential application in bone tissue engineering. Calcium ions quantitation was made using a fused silica capillary (40 cm, 75 µm ID) and background electrolyte containing 5 mM imidazole, 6 mM á-hydroxyisobutyric acid, 1 mM 1,4,7,10,13,16-hexaoxacyclo-octadecane and 20 % w/v methanol, at pH 4.5. Indirect UV-detection mode at 214 nm was performed. The separations were achieved using normal polarity at 6 kV, a cartridge temperature of 25 µC and 0.5 psi for 5 s for sample introduction. Parameters of validation such as specificity, linearity, limit of detection and quantitation, accuracy, precision and robustness were evaluated according to the International Conference on Harmonization guidelines. The new developed method resulted to be suitable for calcium determination from composite biomaterials for potential application in bone tissue engineering.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
info:eu-repo/date/embargoEnd/2015-12-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1924
Cattalini, Juan Pablo; García, Javier; Mouriño, Viviana Silvia Lourdes; Lucangioli, Silvia Edith; Development and validation of a Capillary Zone Electrophoresis method for the determination of calcium in composite biomaterials; Bentham Science Publishers; Current Analytical Chemistry; 10; 4; 10-2014; 465-472
1573-4110
http://www.ingentaconnect.com/content/ben/cac/2014/00000010/00000004/art00004?crawler=true#expand/collapse
url http://hdl.handle.net/11336/1924
http://www.ingentaconnect.com/content/ben/cac/2014/00000010/00000004/art00004?crawler=true#expand/collapse
identifier_str_mv Cattalini, Juan Pablo; García, Javier; Mouriño, Viviana Silvia Lourdes; Lucangioli, Silvia Edith; Development and validation of a Capillary Zone Electrophoresis method for the determination of calcium in composite biomaterials; Bentham Science Publishers; Current Analytical Chemistry; 10; 4; 10-2014; 465-472
1573-4110
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/ 10.2174/15734110113099990038
info:eu-repo/semantics/altIdentifier/url/http://benthamscience.com/journals/current-analytical-chemistry/volume/10/issue/4/page/465/
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv embargoedAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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