Flourishing follicles: Overview of ovarioles

Autores
Kelleher, Andrew M.; Khalaj, Kasra; Martin, Jacinta H.; Scaia, María Florencia; Wilson, Rebecca L.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ovarian follicle development is essential for the propagation of species, and unifies both vertebrates and invertebrates. In Drosophila melanogaster, follicle development occurs through a spectacularly coordinated sequence within the highly polarized ovariole (pictured). A single ovary contains more than a dozen ovarioles that cluster together to form bud-like structures. Gentle mechanical disruption of the ovary allows for the separation and visualization of individual ovarioles and, thus, the progression of follicle development. Here, D. melanogaster ovarioles were isolated and stained with 4´,6-diamidino-2-phenylindole (DAPI) to identify the characteristic stages of follicular maturation. Follicle development occurs in a distinct anterior-to-posterior direction, beginning in the germarium (red) and progressing through a set of egg chambers of increasing numerical stage (green, blue, yellow, and magenta), before terminating an a mature oocyte (cyan). Germ line stem cells, residing within the germarium, are a self-renewing population that divide to form a daughter stem cell, which remains in the germarium, and a cystoblast. The cystoblast traverses through the ovariole, where it undergoes sequential rounds of division to form an egg chamber housing 15 nurse cells that work in tandem to nourish a single developing oocyte. The egg chamber is additionally surrounded by numerous follicular cells. Ultimately, the developmentally competent egg will exit the assembly line seven days later and enter the uterus where it may be fertilized. A fertilized egg activates during its deposition on an exterior surface, allowing and embryonic development to commence externally
Fil: Kelleher, Andrew M.. University of Missouri; Estados Unidos
Fil: Khalaj, Kasra. Queens University; Canadá
Fil: Martin, Jacinta H.. University of Newcastle; Reino Unido
Fil: Scaia, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Wilson, Rebecca L.. University of Adelaide; Australia
Materia
Ovarioles
Drosophila Melanogaster
Reproduction
Follicles
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/63712
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Flourishing follicles: Overview of ovariolesKelleher, Andrew M.Khalaj, KasraMartin, Jacinta H.Scaia, María FlorenciaWilson, Rebecca L.OvariolesDrosophila MelanogasterReproductionFollicleshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Ovarian follicle development is essential for the propagation of species, and unifies both vertebrates and invertebrates. In Drosophila melanogaster, follicle development occurs through a spectacularly coordinated sequence within the highly polarized ovariole (pictured). A single ovary contains more than a dozen ovarioles that cluster together to form bud-like structures. Gentle mechanical disruption of the ovary allows for the separation and visualization of individual ovarioles and, thus, the progression of follicle development. Here, D. melanogaster ovarioles were isolated and stained with 4´,6-diamidino-2-phenylindole (DAPI) to identify the characteristic stages of follicular maturation. Follicle development occurs in a distinct anterior-to-posterior direction, beginning in the germarium (red) and progressing through a set of egg chambers of increasing numerical stage (green, blue, yellow, and magenta), before terminating an a mature oocyte (cyan). Germ line stem cells, residing within the germarium, are a self-renewing population that divide to form a daughter stem cell, which remains in the germarium, and a cystoblast. The cystoblast traverses through the ovariole, where it undergoes sequential rounds of division to form an egg chamber housing 15 nurse cells that work in tandem to nourish a single developing oocyte. The egg chamber is additionally surrounded by numerous follicular cells. Ultimately, the developmentally competent egg will exit the assembly line seven days later and enter the uterus where it may be fertilized. A fertilized egg activates during its deposition on an exterior surface, allowing and embryonic development to commence externallyFil: Kelleher, Andrew M.. University of Missouri; Estados UnidosFil: Khalaj, Kasra. Queens University; CanadáFil: Martin, Jacinta H.. University of Newcastle; Reino UnidoFil: Scaia, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Wilson, Rebecca L.. University of Adelaide; AustraliaWiley-liss, Div John Wiley & Sons Inc2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63712Kelleher, Andrew M.; Khalaj, Kasra; Martin, Jacinta H.; Scaia, María Florencia; Wilson, Rebecca L.; Flourishing follicles: Overview of ovarioles; Wiley-liss, Div John Wiley & Sons Inc; Molecular Reproduction and Development; 84; 12; 12-2017; 1237-12371040-452XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/mrd.22858info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/mrd.22858info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:07Zoai:ri.conicet.gov.ar:11336/63712instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:08.055CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Flourishing follicles: Overview of ovarioles
title Flourishing follicles: Overview of ovarioles
spellingShingle Flourishing follicles: Overview of ovarioles
Kelleher, Andrew M.
Ovarioles
Drosophila Melanogaster
Reproduction
Follicles
title_short Flourishing follicles: Overview of ovarioles
title_full Flourishing follicles: Overview of ovarioles
title_fullStr Flourishing follicles: Overview of ovarioles
title_full_unstemmed Flourishing follicles: Overview of ovarioles
title_sort Flourishing follicles: Overview of ovarioles
dc.creator.none.fl_str_mv Kelleher, Andrew M.
Khalaj, Kasra
Martin, Jacinta H.
Scaia, María Florencia
Wilson, Rebecca L.
author Kelleher, Andrew M.
author_facet Kelleher, Andrew M.
Khalaj, Kasra
Martin, Jacinta H.
Scaia, María Florencia
Wilson, Rebecca L.
author_role author
author2 Khalaj, Kasra
Martin, Jacinta H.
Scaia, María Florencia
Wilson, Rebecca L.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ovarioles
Drosophila Melanogaster
Reproduction
Follicles
topic Ovarioles
Drosophila Melanogaster
Reproduction
Follicles
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Ovarian follicle development is essential for the propagation of species, and unifies both vertebrates and invertebrates. In Drosophila melanogaster, follicle development occurs through a spectacularly coordinated sequence within the highly polarized ovariole (pictured). A single ovary contains more than a dozen ovarioles that cluster together to form bud-like structures. Gentle mechanical disruption of the ovary allows for the separation and visualization of individual ovarioles and, thus, the progression of follicle development. Here, D. melanogaster ovarioles were isolated and stained with 4´,6-diamidino-2-phenylindole (DAPI) to identify the characteristic stages of follicular maturation. Follicle development occurs in a distinct anterior-to-posterior direction, beginning in the germarium (red) and progressing through a set of egg chambers of increasing numerical stage (green, blue, yellow, and magenta), before terminating an a mature oocyte (cyan). Germ line stem cells, residing within the germarium, are a self-renewing population that divide to form a daughter stem cell, which remains in the germarium, and a cystoblast. The cystoblast traverses through the ovariole, where it undergoes sequential rounds of division to form an egg chamber housing 15 nurse cells that work in tandem to nourish a single developing oocyte. The egg chamber is additionally surrounded by numerous follicular cells. Ultimately, the developmentally competent egg will exit the assembly line seven days later and enter the uterus where it may be fertilized. A fertilized egg activates during its deposition on an exterior surface, allowing and embryonic development to commence externally
Fil: Kelleher, Andrew M.. University of Missouri; Estados Unidos
Fil: Khalaj, Kasra. Queens University; Canadá
Fil: Martin, Jacinta H.. University of Newcastle; Reino Unido
Fil: Scaia, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Wilson, Rebecca L.. University of Adelaide; Australia
description Ovarian follicle development is essential for the propagation of species, and unifies both vertebrates and invertebrates. In Drosophila melanogaster, follicle development occurs through a spectacularly coordinated sequence within the highly polarized ovariole (pictured). A single ovary contains more than a dozen ovarioles that cluster together to form bud-like structures. Gentle mechanical disruption of the ovary allows for the separation and visualization of individual ovarioles and, thus, the progression of follicle development. Here, D. melanogaster ovarioles were isolated and stained with 4´,6-diamidino-2-phenylindole (DAPI) to identify the characteristic stages of follicular maturation. Follicle development occurs in a distinct anterior-to-posterior direction, beginning in the germarium (red) and progressing through a set of egg chambers of increasing numerical stage (green, blue, yellow, and magenta), before terminating an a mature oocyte (cyan). Germ line stem cells, residing within the germarium, are a self-renewing population that divide to form a daughter stem cell, which remains in the germarium, and a cystoblast. The cystoblast traverses through the ovariole, where it undergoes sequential rounds of division to form an egg chamber housing 15 nurse cells that work in tandem to nourish a single developing oocyte. The egg chamber is additionally surrounded by numerous follicular cells. Ultimately, the developmentally competent egg will exit the assembly line seven days later and enter the uterus where it may be fertilized. A fertilized egg activates during its deposition on an exterior surface, allowing and embryonic development to commence externally
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/63712
Kelleher, Andrew M.; Khalaj, Kasra; Martin, Jacinta H.; Scaia, María Florencia; Wilson, Rebecca L.; Flourishing follicles: Overview of ovarioles; Wiley-liss, Div John Wiley & Sons Inc; Molecular Reproduction and Development; 84; 12; 12-2017; 1237-1237
1040-452X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/63712
identifier_str_mv Kelleher, Andrew M.; Khalaj, Kasra; Martin, Jacinta H.; Scaia, María Florencia; Wilson, Rebecca L.; Flourishing follicles: Overview of ovarioles; Wiley-liss, Div John Wiley & Sons Inc; Molecular Reproduction and Development; 84; 12; 12-2017; 1237-1237
1040-452X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/mrd.22858
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/mrd.22858
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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