Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction

Autores
Ghio, Silvina; Sabarís Di Lorenzo, Gonzalo Julián; Lia, Verónica Viviana; Talia, Paola Monica; Cataldi, Angel Adrian; Grasso, Daniel Horacio; Campos, Eleonora
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Prospection of cellulose-degrading bacteria in natural environments allows the identification of novel cellulases
and hemicellulases that could be useful in second-generation bioethanol production. In this work, cellulolytic
bacteria were isolated from decaying native forest soils by enrichment on cellulose as sole carbon source. There
was a predominance of Gram positive isolates that belonged to the phyla Proteobacteria and Firmicutes. Many
primary isolates with cellulolytic activity were not pure cultures. From these consortia, isolation of pure constituents
was attempted in order to test the hypothesis whether microbial consortia are needed for full degradation of
complex substrates. Two isolates, CB1-2-A-5 and VG-4-A-2, were obtained as the pure constituents of CB1-2 and
VG-4 consortia, respectively. Based on 16S RNA sequence, they could be classified as Variovorax paradoxus and
Paenibacillus alvei. Noteworthy, only VG-4 consortium showed measurable xylan degrading capacity and signs of
filter paper degradation. However, no xylan or filter paper degrading capacities were observed for the pure cultures
isolated from it, suggesting that other members of this consortium were necessary for these hydrolyzing activities.
Our results indicated that Paenibacillus sp. and Variovorax sp. as well as VG-4 consortium, might be a useful source
of hydrolytic enzymes. Moreover, although Variovorax sp. had been previously identified in metagenomic studies
of cellulolytic communities, this is the first report on the isolation and characterization of this microorganism as a
cellulolytic genus.
Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; Argentina
Fil: Sabarís Di Lorenzo, Gonzalo Julián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Lia, Verónica Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Talia, Paola Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Grasso, Daniel Horacio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
cellulolytic bacteria
Variovorax
Paenibacillus
soil
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/271375
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/271375
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstructionGhio, SilvinaSabarís Di Lorenzo, Gonzalo JuliánLia, Verónica VivianaTalia, Paola MonicaCataldi, Angel AdrianGrasso, Daniel HoracioCampos, Eleonoracellulolytic bacteriaVariovoraxPaenibacillussoilhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Prospection of cellulose-degrading bacteria in natural environments allows the identification of novel cellulases<br />and hemicellulases that could be useful in second-generation bioethanol production. In this work, cellulolytic<br />bacteria were isolated from decaying native forest soils by enrichment on cellulose as sole carbon source. There<br />was a predominance of Gram positive isolates that belonged to the phyla Proteobacteria and Firmicutes. Many<br />primary isolates with cellulolytic activity were not pure cultures. From these consortia, isolation of pure constituents<br />was attempted in order to test the hypothesis whether microbial consortia are needed for full degradation of<br />complex substrates. Two isolates, CB1-2-A-5 and VG-4-A-2, were obtained as the pure constituents of CB1-2 and<br />VG-4 consortia, respectively. Based on 16S RNA sequence, they could be classified as Variovorax paradoxus and<br />Paenibacillus alvei. Noteworthy, only VG-4 consortium showed measurable xylan degrading capacity and signs of<br />filter paper degradation. However, no xylan or filter paper degrading capacities were observed for the pure cultures<br />isolated from it, suggesting that other members of this consortium were necessary for these hydrolyzing activities.<br />Our results indicated that Paenibacillus sp. and Variovorax sp. as well as VG-4 consortium, might be a useful source<br />of hydrolytic enzymes. Moreover, although Variovorax sp. had been previously identified in metagenomic studies<br />of cellulolytic communities, this is the first report on the isolation and characterization of this microorganism as a<br />cellulolytic genus.Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; ArgentinaFil: Sabarís Di Lorenzo, Gonzalo Julián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Lia, Verónica Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Talia, Paola Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Grasso, Daniel Horacio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; ArgentinaFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinae-Century Publishing Corporation2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271375Ghio, Silvina; Sabarís Di Lorenzo, Gonzalo Julián; Lia, Verónica Viviana; Talia, Paola Monica; Cataldi, Angel Adrian; et al.; Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction; e-Century Publishing Corporation; International Journal of Biochemistry and Molecular Biology; 3; 4; 12-2012; 352-3642152-4114CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ijbmb.org/files/ijbmb1211003.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:16Zoai:ri.conicet.gov.ar:11336/271375instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:16.947CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction
title Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction
spellingShingle Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction
Ghio, Silvina
cellulolytic bacteria
Variovorax
Paenibacillus
soil
title_short Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction
title_full Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction
title_fullStr Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction
title_full_unstemmed Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction
title_sort Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction
dc.creator.none.fl_str_mv Ghio, Silvina
Sabarís Di Lorenzo, Gonzalo Julián
Lia, Verónica Viviana
Talia, Paola Monica
Cataldi, Angel Adrian
Grasso, Daniel Horacio
Campos, Eleonora
author Ghio, Silvina
author_facet Ghio, Silvina
Sabarís Di Lorenzo, Gonzalo Julián
Lia, Verónica Viviana
Talia, Paola Monica
Cataldi, Angel Adrian
Grasso, Daniel Horacio
Campos, Eleonora
author_role author
author2 Sabarís Di Lorenzo, Gonzalo Julián
Lia, Verónica Viviana
Talia, Paola Monica
Cataldi, Angel Adrian
Grasso, Daniel Horacio
Campos, Eleonora
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv cellulolytic bacteria
Variovorax
Paenibacillus
soil
topic cellulolytic bacteria
Variovorax
Paenibacillus
soil
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Prospection of cellulose-degrading bacteria in natural environments allows the identification of novel cellulases<br />and hemicellulases that could be useful in second-generation bioethanol production. In this work, cellulolytic<br />bacteria were isolated from decaying native forest soils by enrichment on cellulose as sole carbon source. There<br />was a predominance of Gram positive isolates that belonged to the phyla Proteobacteria and Firmicutes. Many<br />primary isolates with cellulolytic activity were not pure cultures. From these consortia, isolation of pure constituents<br />was attempted in order to test the hypothesis whether microbial consortia are needed for full degradation of<br />complex substrates. Two isolates, CB1-2-A-5 and VG-4-A-2, were obtained as the pure constituents of CB1-2 and<br />VG-4 consortia, respectively. Based on 16S RNA sequence, they could be classified as Variovorax paradoxus and<br />Paenibacillus alvei. Noteworthy, only VG-4 consortium showed measurable xylan degrading capacity and signs of<br />filter paper degradation. However, no xylan or filter paper degrading capacities were observed for the pure cultures<br />isolated from it, suggesting that other members of this consortium were necessary for these hydrolyzing activities.<br />Our results indicated that Paenibacillus sp. and Variovorax sp. as well as VG-4 consortium, might be a useful source<br />of hydrolytic enzymes. Moreover, although Variovorax sp. had been previously identified in metagenomic studies<br />of cellulolytic communities, this is the first report on the isolation and characterization of this microorganism as a<br />cellulolytic genus.
Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; Argentina
Fil: Sabarís Di Lorenzo, Gonzalo Julián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Lia, Verónica Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Talia, Paola Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Grasso, Daniel Horacio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Prospection of cellulose-degrading bacteria in natural environments allows the identification of novel cellulases<br />and hemicellulases that could be useful in second-generation bioethanol production. In this work, cellulolytic<br />bacteria were isolated from decaying native forest soils by enrichment on cellulose as sole carbon source. There<br />was a predominance of Gram positive isolates that belonged to the phyla Proteobacteria and Firmicutes. Many<br />primary isolates with cellulolytic activity were not pure cultures. From these consortia, isolation of pure constituents<br />was attempted in order to test the hypothesis whether microbial consortia are needed for full degradation of<br />complex substrates. Two isolates, CB1-2-A-5 and VG-4-A-2, were obtained as the pure constituents of CB1-2 and<br />VG-4 consortia, respectively. Based on 16S RNA sequence, they could be classified as Variovorax paradoxus and<br />Paenibacillus alvei. Noteworthy, only VG-4 consortium showed measurable xylan degrading capacity and signs of<br />filter paper degradation. However, no xylan or filter paper degrading capacities were observed for the pure cultures<br />isolated from it, suggesting that other members of this consortium were necessary for these hydrolyzing activities.<br />Our results indicated that Paenibacillus sp. and Variovorax sp. as well as VG-4 consortium, might be a useful source<br />of hydrolytic enzymes. Moreover, although Variovorax sp. had been previously identified in metagenomic studies<br />of cellulolytic communities, this is the first report on the isolation and characterization of this microorganism as a<br />cellulolytic genus.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/271375
Ghio, Silvina; Sabarís Di Lorenzo, Gonzalo Julián; Lia, Verónica Viviana; Talia, Paola Monica; Cataldi, Angel Adrian; et al.; Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction; e-Century Publishing Corporation; International Journal of Biochemistry and Molecular Biology; 3; 4; 12-2012; 352-364
2152-4114
CONICET Digital
CONICET
url http://hdl.handle.net/11336/271375
identifier_str_mv Ghio, Silvina; Sabarís Di Lorenzo, Gonzalo Julián; Lia, Verónica Viviana; Talia, Paola Monica; Cataldi, Angel Adrian; et al.; Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction; e-Century Publishing Corporation; International Journal of Biochemistry and Molecular Biology; 3; 4; 12-2012; 352-364
2152-4114
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ijbmb.org/files/ijbmb1211003.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
application/pdf
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dc.publisher.none.fl_str_mv e-Century Publishing Corporation
publisher.none.fl_str_mv e-Century Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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